(Circular) (Six-base) MAPSORT of: pFN21AB7823.seq Check: 796 from: 1 to: 6313 pFN21AB7823 6313 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3263 3263 Size: 6313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3454 3656 1937 Size: 1517 202 4594 Fragments arranged by size: 4594 1517 202 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2085 2301 3128 4376 5760 6028 1632 Size: 453 216 827 1248 1384 268 1917 Fragments arranged by size: 1917 1384 1248 827 453 268 216 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 3375 5212 6189 1203 Size: 2172 1837 977 1327 Fragments arranged by size: 2172 1837 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6313 AlwNI CAG_nnn'CTG Cuts at: 3125 4056 5628 3125 Size: 931 1572 3810 Fragments arranged by size: 3810 1572 931 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 2655 2851 3288 3622 3848 4000 6109 1493 Size: 1162 196 437 334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1162 437 334 226 196 152 AseI AT'TA_AT Cuts at: 160 1032 2646 4867 160 Size: 872 1614 2221 1606 Fragments arranged by size: 2221 1614 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 2661 3638 1870 Size: 73 718 977 4545 Fragments arranged by size: 4545 977 718 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2147 2180 2147 Size: 33 6280 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3634 5089 618 Size: 325 217 627 1847 1455 1842 Fragments arranged by size: 1847 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3632 1391 Size: 2241 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2132 2148 2229 4205 5415 2132 Size: 16 81 1976 1210 3030 Fragments arranged by size: 3030 1976 1210 81 16 BclI T'GATC_A Cuts at: 1548 3304 1548 Size: 1756 4557 BfrBI ATG'CAT Cuts at: 2695 2695 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2864 4924 136 Size: 107 122 71 631 605 1192 2060 1525 Fragments arranged by size: 2060 1525 1192 631 605 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 2923 4374 5530 1790 Size: 1133 1451 1156 2573 Fragments arranged by size: 2573 1451 1156 1133 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2209 3348 4044 Size: 416 53 83 399 24 792 1139 696 Cuts at: 4044 5002 442 Size: 958 1753 Fragments arranged by size: 1753 1139 958 792 696 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2595 3334 4973 1239 Size: 1356 739 1639 2579 Fragments arranged by size: 2579 1639 1356 739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2078 4438 5303 5601 2078 Size: 2360 865 298 2790 Fragments arranged by size: 2790 2360 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2113 4976 882 Size: 617 614 2863 2219 Fragments arranged by size: 2863 2219 617 614 BsaAI yAC'GTr Cuts at: 493 1204 3053 4104 493 Size: 711 1849 1051 2702 Fragments arranged by size: 2702 1849 1051 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4120 4502 5137 275 Size: 53 83 186 936 2587 382 635 1451 Fragments arranged by size: 2587 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 3102 4746 5144 5418 5565 5976 2142 Size: 960 1644 398 274 147 411 2479 Fragments arranged by size: 2479 1644 960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3307 3337 1178 Size: 30 165 30 1904 30 4154 Fragments arranged by size: 4154 1904 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2667 3616 1186 Size: 915 566 949 3883 Fragments arranged by size: 3883 949 915 566 BseYI C'CCAG_C Cuts at: 3011 5516 6012 3011 Size: 2505 496 3312 Fragments arranged by size: 3312 2505 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2013 1163 Size: 573 277 5463 Fragments arranged by size: 5463 573 277 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4374 4459 5530 4374 Size: 85 1071 5157 Fragments arranged by size: 5157 1071 85 BsiWI C'GTAC_G Cuts at: 2133 2133 Size: 6313 BsmI GAATG_Cn' Cuts at: 2260 2386 2564 2695 3824 3917 2260 Size: 126 178 131 1129 93 4656 Fragments arranged by size: 4656 1129 178 131 126 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2923 3632 4374 4459 5530 1391 Size: 399 1133 709 742 85 1071 2174 Fragments arranged by size: 2174 1133 1071 742 709 399 85 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2035 2406 3263 3668 844 Size: 1191 371 857 405 3489 Fragments arranged by size: 3489 1191 857 405 371 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2880 4808 4982 65 Size: 1183 1632 1928 174 1396 Fragments arranged by size: 1928 1632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 2898 3581 4373 5385 2898 Size: 683 792 1012 3826 Fragments arranged by size: 3826 1012 792 683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 2272 3256 2272 Size: 984 5329 BstYI r'GATC_y Cuts at: 1972 2078 2202 2617 3493 3643 4010 4395 4412 Size: 106 124 415 876 150 367 385 17 Cuts at: 4412 5864 5950 5962 6308 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 876 415 385 367 346 150 124 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 3829 4623 4643 3829 Size: 794 20 5499 Fragments arranged by size: 5499 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2717 2752 529 Size: 35 2153 35 4090 Fragments arranged by size: 4090 2153 35 35 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 3760 4649 Size: 54 1425 50 275 18 432 1498 889 Cuts at: 4649 6278 8 Size: 1629 43 Fragments arranged by size: 1629 1498 1425 889 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2113 2815 2961 3001 3242 3593 4243 1353 Size: 760 702 146 40 241 351 650 3423 Fragments arranged by size: 3423 760 702 650 351 241 146 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2301 2595 3128 3334 4376 4973 Size: 393 453 216 294 533 206 1042 597 Cuts at: 4973 5760 6028 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1042 787 597 533 453 393 294 268 216 206 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 EcoRV GAT'ATC Cuts at: 3166 3166 Size: 6313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2990 2990 Size: 6313 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 2012 5460 1678 Size: 334 3448 2531 Fragments arranged by size: 3448 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3000 3007 3032 3039 3119 Size: 32 135 32 1428 7 25 7 80 Cuts at: 3119 3151 4954 4986 5028 5060 1373 Size: 32 1803 32 42 32 2626 Fragments arranged by size: 2626 1803 1428 135 80 42 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6313 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 2304 3912 2304 Size: 1608 4705 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 5611 1305 Size: 4122 184 2007 Fragments arranged by size: 4122 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 10 Size: 54 1425 343 432 4059 Fragments arranged by size: 4059 1425 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2844 3585 4271 4630 4789 518 Size: 648 1104 574 741 686 359 159 2042 Fragments arranged by size: 2042 1104 741 686 648 574 359 159 MspA1I CmG'CkG Cuts at: 1606 2507 3729 4406 5554 5799 1606 Size: 901 1222 677 1148 245 2120 Fragments arranged by size: 2120 1222 1148 901 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4822 Fragments arranged by size: 4822 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NsiI A_TGCA'T Cuts at: 2697 2697 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 3379 3671 5216 1132 Size: 97 2150 292 1545 2229 Fragments arranged by size: 2229 2150 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 3375 5212 3375 Size: 1837 4476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PmlI CAC'GTG Cuts at: 3053 3053 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2802 1613 Size: 1189 5124 PsiI TTA'TAA Cuts at: 3294 3883 3294 Size: 589 5724 PstI C_TGCA'G Cuts at: 830 3665 830 Size: 2835 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 2507 2507 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SapI GCTCTTCn'nnn_ Cuts at: 3001 3242 3001 Size: 241 6072 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SexAI A'CCwGG_T Cuts at: 2358 2358 Size: 6313 SfcI C'TryA_G Cuts at: 826 1045 2828 3661 4796 5477 5668 826 Size: 219 1783 833 1135 681 191 1471 Fragments arranged by size: 1783 1471 1135 833 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 2663 3640 1872 Size: 791 977 4545 Fragments arranged by size: 4545 977 791 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 4417 5318 5580 820 Size: 197 926 114 2360 901 262 1553 Fragments arranged by size: 2360 1553 926 901 262 197 114 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6313 StyI C'CwwG_G Cuts at: 513 1065 2004 2633 3249 3573 3752 513 Size: 552 939 629 616 324 179 3074 Fragments arranged by size: 3074 939 629 616 552 324 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4340 4357 4510 4695 5140 325 Size: 1255 2760 17 153 185 445 1498 Fragments arranged by size: 2760 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2836 4559 6271 Size: 274 80 33 51 1443 859 1723 1712 Cuts at: 6271 96 Size: 138 Fragments arranged by size: 1723 1712 1443 859 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2884 2916 6273 6305 2884 Size: 32 3357 32 2892 Fragments arranged by size: 3357 2892 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2333 1316 Size: 1017 5296 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3361 3361 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 2661 3638 1870 Size: 791 977 4545 Fragments arranged by size: 4545 977 791 XmnI GAAnn'nnTTC Cuts at: 4442 4442 Size: 6313 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI