Restriction map for
pFN21AB6793
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstNI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT22I FauI FbaI FseI
HaeII Hin1I HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MroI MseI MunI MvaI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 264 CGCG CG^CG
AfaI 145 GTAC GT^AC
AluI 87 AGCT AG^CT
Asp700I 15 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 284 TGCGCA TGC^GCA
BalI 230 TGGCCA TGG^CCA
BcnI 275 CCGGG CC^[GC]GG
BsmAI 377 GAGAC GTCTC(1/5)
Bsp1407I 144 TGTACA T^GTACA
BsrGI 144 TGTACA T^GTACA
BssKI 275 CCGGG ^CC[ACGT]GG
BstEII 267 GGTCACC G^GT[ACGT]ACC
BstPI 267 GGTCACC G^GT[ACGT]ACC
BstUI 264 CGCG CG^CG
BstXI 236 CCAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 183 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 285 GCGC GCG^C
Cfr13I 363 GGCCC G^G[ACGT]CC
DsaI 242 CCGTGG C^C[GA][CT]GG
EcoO65I 267 GGTCACC G^GT[ACGT]ACC
FspI 284 TGCGCA TGC^GCA
HapII 275 CCGG C^CGG
HgaI 218 GCGTC GACGC(5/10)
HhaI 285 GCGC GCG^C
HinP1I 285 GCGC G^CGC
HincII 220 GTCAAC GT[CT]^[GA]AC
HindII 220 GTCAAC GT[CT]^[GA]AC
HpaII 275 CCGG C^CGG
MluNI 230 TGGCCA TGG^CCA
MscI 230 TGGCCA TGG^CCA
MslI 111 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 86 CAGCTG C[AC]G^C[GT]G
MspI 275 CCGG C^CGG
MvnI 264 CGCG CG^CG
MwoI 355 GCAGAGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 275 CCGGG CC^[GC]GG
NlaIII 111 CATG CATG^
NlaIV 121 GGAGCC GG[ACGT]^[ACGT]CC
PvuII 86 CAGCTG CAG^CTG
RsaI 145 GTAC GT^AC
Sau96I 363 GGCCC G^G[ACGT]CC
ScrFI 275 CCGGG CC^[ACGT]GG
SfaNI 331 GCATC GCATC(5/9)
SmlI 297 CTTGAG C^T[CT][GA]AG
SspBI 144 TGTACA T^GTACA
XmnI 15 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BbvI 85 GCAGC GCAGC(8/12)
215 GCAGC
BseRI 247 GAGGAG GAGGAG(10/8)
358 GAGGAG
BstF5I 332 CATCC GGATG(2/0)
3 GGATG
DdeI 89 CTGAG C^T[ACGT]AG
159 CTCAG
EaeI 164 TGGCCG [CT]^GGCC[GA]
230 TGGCCA
Fnu4HI 85 GCAGC GC^[ACGT]GC
215 GCAGC
FokI 332 CATCC GGATG(9/13)
3 GGATG
ItaI 85 GCAGC GC^[ACGT]GC
215 GCAGC
TseI 85 GCAGC G^C[AT]GC
215 GCAGC
TspRI 161 CAGTG CA[GC]TG[ACGT][ACGT]^
225 CACTG
XcmI 32 CCAAGGAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
233 CCACCAAGACCGTGG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BbsI BpuAI BsaJI BsiYI
BslI EcoT14I HaeIII HphI MaeIII
MboII MnlI StyI Tsp45I