(Circular) (Six-base) MAPSORT of: pFN21AE0038.seq Check: 2305 from: 1 to: 6811 pFN21AE0038 6811 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4621 278 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3876 4154 1937 Size: 1939 278 4594 Fragments arranged by size: 4594 1939 278 Acc65I G'GTAC_C Cuts at: 3659 4132 3659 Size: 473 6338 AclI AA'CG_TT Cuts at: 1563 4110 4938 5311 1563 Size: 2547 828 373 3063 Fragments arranged by size: 3063 2547 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 4874 6258 6526 1632 Size: 402 2840 1384 268 1917 Fragments arranged by size: 2840 1917 1384 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6614 AflIII A'CryG_T Cuts at: 1203 2511 3797 4073 5710 6687 1203 Size: 1308 1286 276 1637 977 1327 Fragments arranged by size: 1637 1327 1308 1286 977 276 AgeI A'CCGG_T Cuts at: 5642 5642 Size: 6811 AhdI GACnn_n'nnGTC Cuts at: 5540 5540 Size: 6811 AleI CACnn'nnGTG Cuts at: 1166 2512 3567 1166 Size: 1346 1055 4410 Fragments arranged by size: 4410 1346 1055 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4113 1184 Size: 2929 3882 AlwNI CAG_nnn'CTG Cuts at: 4554 6126 4554 Size: 1572 5239 ApaLI G'TGCA_C Cuts at: 4868 6024 4868 Size: 1156 5655 ApoI r'AATT_y Cuts at: 1493 2151 2233 2404 2877 3200 3230 3602 3756 Size: 658 82 171 473 323 30 372 154 Cuts at: 3756 3953 4019 4120 4346 4498 6607 1493 Size: 197 66 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 658 473 372 323 226 197 171 154 152 101 82 66 30 AseI AT'TA_AT Cuts at: 160 1032 2391 2436 5365 160 Size: 872 1359 45 2929 1606 Fragments arranged by size: 2929 1606 1359 872 45 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 AvaI C'yCGr_G Cuts at: 1870 1943 4136 1870 Size: 73 2193 4545 Fragments arranged by size: 4545 2193 73 BamHI G'GATC_C Cuts at: 4141 4508 4141 Size: 367 6444 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3659 4132 5587 618 Size: 325 217 627 1872 473 1455 1842 Fragments arranged by size: 1872 1842 1455 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 3784 4130 1391 Size: 2393 346 4072 Fragments arranged by size: 4072 2393 346 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2518 928 Size: 971 619 5221 Fragments arranged by size: 5221 971 619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5002 5036 1903 Size: 34 3065 34 3678 Fragments arranged by size: 3678 3065 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4703 5913 4703 Size: 1210 5601 BclI T'GATC_A Cuts at: 1548 1548 Size: 6811 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5422 136 Size: 107 122 71 631 605 3750 1525 Fragments arranged by size: 3750 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2725 6806 2725 Size: 4081 2730 BlpI GC'TnA_GC Cuts at: 4228 4228 Size: 6811 Bme1580I G_kGCm'C Cuts at: 1790 4872 6028 1790 Size: 3082 1156 2573 Fragments arranged by size: 3082 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6811 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2690 4542 5500 Size: 416 53 83 399 24 1273 1852 958 Cuts at: 5500 442 Size: 1753 Fragments arranged by size: 1852 1753 1273 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6811 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3524 5471 1239 Size: 2285 1947 2579 Fragments arranged by size: 2579 2285 1947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4936 5801 6099 3463 Size: 1473 865 298 4175 Fragments arranged by size: 4175 1473 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3542 5474 882 Size: 617 2043 1932 2219 Fragments arranged by size: 2219 2043 1932 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 4602 493 Size: 711 1503 1895 2702 Fragments arranged by size: 2702 1895 1503 711 BsaBI GATnn'nnATC Cuts at: 2919 3472 6805 2919 Size: 553 3333 2925 Fragments arranged by size: 3333 2925 553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4618 5000 5635 275 Size: 53 83 186 936 3085 382 635 1451 Fragments arranged by size: 3085 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5244 5642 5916 6063 6474 5244 Size: 398 274 147 411 5581 Fragments arranged by size: 5581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3086 3116 1178 Size: 30 165 30 1683 30 4873 Fragments arranged by size: 4873 1683 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6811 BseYI C'CCAG_C Cuts at: 3907 6014 6510 3907 Size: 2107 496 4208 Fragments arranged by size: 4208 2107 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4261 5022 6050 1540 Size: 275 186 2260 761 1028 2301 Fragments arranged by size: 2301 2260 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2735 3832 4872 4957 6028 2735 Size: 1097 1040 85 1071 3518 Fragments arranged by size: 3518 1097 1071 1040 85 BsmI GAATG_Cn' Cuts at: 2647 3578 4322 4415 2647 Size: 931 744 93 5043 Fragments arranged by size: 5043 931 744 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2735 3784 3832 4130 4872 4957 6028 Size: 399 945 1049 48 298 742 85 1071 Cuts at: 6028 1391 Size: 2174 Fragments arranged by size: 2174 1071 1049 945 742 399 298 85 48 BspEI T'CCGG_A Cuts at: 6474 6474 Size: 6811 BspHI T'CATG_A Cuts at: 4700 6430 4700 Size: 1730 5081 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4166 844 Size: 3322 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4698 1394 Size: 429 2875 3507 Fragments arranged by size: 3507 2875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 2888 3723 5306 5480 65 Size: 1183 835 805 835 1583 174 1396 Fragments arranged by size: 1583 1396 1183 835 835 805 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3311 5455 5642 1700 Size: 110 1501 2144 187 2869 Fragments arranged by size: 2869 2144 1501 187 110 BsrGI T'GTAC_A Cuts at: 96 3593 96 Size: 3497 3314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6811 BssSI C'ACGA_G Cuts at: 4871 5883 4871 Size: 1012 5799 BstAPI GCAn_nnn'nTGC Cuts at: 794 3122 794 Size: 2328 4483 BstBI TT'CG_AA Cuts at: 5615 5615 Size: 6811 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6811 BstYI r'GATC_y Cuts at: 1972 2267 2517 2684 2725 4141 4508 4893 4910 Size: 295 250 167 41 1416 367 385 17 Cuts at: 4910 6362 6448 6460 6806 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1416 385 367 346 295 250 167 86 41 17 12 Bsu36I CC'TnA_GG Cuts at: 2751 2751 Size: 6811 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5320 Fragments arranged by size: 5320 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6072 Fragments arranged by size: 6072 683 56 BtsI GCAGTG_nn' Cuts at: 4327 5121 5141 4327 Size: 794 20 5997 Fragments arranged by size: 5997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6811 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3805 3840 529 Size: 35 3241 35 3500 Fragments arranged by size: 3500 3241 35 35 DraI TTT'AAA Cuts at: 3085 4115 3085 Size: 1030 5781 DraIII CAC_nnn'GTG Cuts at: 2135 2135 Size: 6811 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5818 809 Size: 507 4502 1802 Fragments arranged by size: 4502 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4258 5147 6776 Size: 54 1425 50 275 18 2428 889 1629 Cuts at: 6776 8 Size: 43 Fragments arranged by size: 2428 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4258 1537 Size: 275 2446 4090 Fragments arranged by size: 4090 2446 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2197 4741 1353 Size: 844 2544 3423 Fragments arranged by size: 3423 2544 844 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5398 5772 5918 387 Size: 1064 138 3809 374 146 1280 Fragments arranged by size: 3809 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 3524 4874 5471 6258 6526 1239 Size: 393 402 1490 1350 597 787 268 1524 Fragments arranged by size: 1524 1490 1350 787 597 402 393 268 EcoRI G'AATT_C Cuts at: 3756 4019 4120 4498 3756 Size: 263 101 378 6069 Fragments arranged by size: 6069 378 263 101 EcoRV GAT'ATC Cuts at: 3255 3255 Size: 6811 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2110 2110 Size: 6811 FspI TGC'GCA Cuts at: 5317 5317 Size: 6811 HaeII r_GCGC'y Cuts at: 1678 5958 1678 Size: 4280 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2174 2206 3174 Size: 32 135 32 458 32 112 32 968 Cuts at: 3174 3206 5452 5484 5526 5558 1373 Size: 32 2246 32 42 32 2626 Fragments arranged by size: 2626 2246 968 458 135 112 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3678 4155 4401 669 Size: 988 281 1740 477 246 3079 Fragments arranged by size: 3079 1740 988 477 281 246 HindIII A'AGCT_T Cuts at: 748 2218 748 Size: 1470 5341 HpaI GTT'AAC Cuts at: 4401 4401 Size: 6811 KpnI G_GTAC'C Cuts at: 3663 4136 3663 Size: 473 6338 MfeI C'AATT_G Cuts at: 3394 4410 3394 Size: 1016 5795 MluI A'CGCG_T Cuts at: 6687 6687 Size: 6811 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2749 3199 5925 6109 1305 Size: 1444 450 2726 184 2007 Fragments arranged by size: 2726 2007 1444 450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4989 Fragments arranged by size: 4989 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2512 3360 3567 4769 5128 5287 518 Size: 648 1346 848 207 1202 359 159 2042 Fragments arranged by size: 2042 1346 1202 848 648 359 207 159 MspA1I CmG'CkG Cuts at: 1606 3822 4227 4904 6052 6297 1606 Size: 2216 405 677 1148 245 2120 Fragments arranged by size: 2216 2120 1148 677 405 245 NaeI GCC'GGC Cuts at: 3313 3313 Size: 6811 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5320 Fragments arranged by size: 5320 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6811 NgoMIV G'CCGG_C Cuts at: 3311 3311 Size: 6811 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6811 NotI GC'GGCC_GC Cuts at: 4258 4258 Size: 6811 NspI r_CATG'y Cuts at: 1132 1229 2515 2897 3801 4077 4090 4169 5714 Size: 97 1286 382 904 276 13 79 1545 Cuts at: 5714 1132 Size: 2229 Fragments arranged by size: 2229 1545 1286 904 382 276 97 79 13 PacI TTA_AT'TAA Cuts at: 2436 2436 Size: 6811 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6811 PciI A'CATG_T Cuts at: 2511 3797 4073 5710 2511 Size: 1286 276 1637 3612 Fragments arranged by size: 3612 1637 1286 276 PflMI CCAn_nnn'nTGG Cuts at: 1108 2669 3425 3733 1108 Size: 1561 756 308 4186 Fragments arranged by size: 4186 1561 756 308 PmeI GTTT'AAAC Cuts at: 4115 4115 Size: 6811 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4880 4912 6393 6425 4880 Size: 32 1481 32 5266 Fragments arranged by size: 5266 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6811 PsiI TTA'TAA Cuts at: 3510 3740 4381 3510 Size: 230 641 5940 Fragments arranged by size: 5940 641 230 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3536 3568 4029 4061 3536 Size: 32 461 32 6286 Fragments arranged by size: 6286 461 32 32 PstI C_TGCA'G Cuts at: 830 4163 830 Size: 3333 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6811 PvuII CAG'CTG Cuts at: 3822 3822 Size: 6811 SalI G'TCGA_C Cuts at: 1936 4153 1936 Size: 2217 4594 SbfI CC_TGCA'GG Cuts at: 4163 4163 Size: 6811 ScaI AGT'ACT Cuts at: 5059 6771 5059 Size: 1712 5099 SfcI C'TryA_G Cuts at: 826 1045 3514 3891 4159 5294 5975 6166 826 Size: 219 2469 377 268 1135 681 191 1471 Fragments arranged by size: 2469 1471 1135 681 377 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6811 SmaI CCC'GGG Cuts at: 1872 4138 1872 Size: 2266 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4915 5816 6078 820 Size: 197 926 1535 1437 901 262 1553 Fragments arranged by size: 1553 1535 1437 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4602 493 Size: 4109 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6811 SphI G_CATG'C Cuts at: 1132 4169 1132 Size: 3037 3774 SspI AAT'ATT Cuts at: 5 52 4735 5 Size: 47 4683 2081 Fragments arranged by size: 4683 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6811 StyI C'CwwG_G Cuts at: 513 1065 2004 2944 3972 4250 513 Size: 552 939 940 1028 278 3074 Fragments arranged by size: 3074 1028 940 939 552 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2299 3557 4838 4855 5008 5193 5638 Size: 1255 719 1258 1281 17 153 185 445 Cuts at: 5638 325 Size: 1498 Fragments arranged by size: 1498 1281 1258 1255 719 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3593 5057 6769 Size: 274 80 33 51 1443 1616 1464 1712 Cuts at: 6769 96 Size: 138 Fragments arranged by size: 1712 1616 1464 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6771 6803 6771 Size: 32 6779 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6811 XbaI T'CTAG_A Cuts at: 4147 4147 Size: 6811 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2572 Size: 6811 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6811 XmaI C'CCGG_G Cuts at: 1870 4136 1870 Size: 2266 4545 XmnI GAAnn'nnTTC Cuts at: 2878 3340 3605 4940 2878 Size: 462 265 1335 4749 Fragments arranged by size: 4749 1335 462 265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4619 276 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI