(Circular) (Six-base) MAPSORT of: pFN21AE0346.seq Check: 4672 from: 1 to: 6610 pFN21AE0346 6610 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2476 2476 Size: 6610 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2099 2763 4420 278 Size: 53 83 186 936 563 664 1657 2468 Fragments arranged by size: 2468 1657 936 664 563 186 83 53 AccI GT'mk_AC Cuts at: 1937 3953 1937 Size: 2016 4594 Acc65I G'GTAC_C Cuts at: 3931 3931 Size: 6610 AclI AA'CG_TT Cuts at: 1563 4737 5110 1563 Size: 3174 373 3063 Fragments arranged by size: 3174 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 3398 4673 6057 6325 1632 Size: 455 1311 1275 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1275 455 268 AfeI AGC'GCT Cuts at: 2674 2916 2674 Size: 242 6368 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6413 AflIII A'CryG_T Cuts at: 1203 2287 5509 6486 1203 Size: 1084 3222 977 1327 Fragments arranged by size: 3222 1327 1084 977 AgeI A'CCGG_T Cuts at: 5441 5441 Size: 6610 AhdI GACnn_n'nnGTC Cuts at: 3166 5339 3166 Size: 2173 4437 AleI CACnn'nnGTG Cuts at: 1166 3215 1166 Size: 2049 4561 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2062 2281 1184 Size: 878 219 5513 Fragments arranged by size: 5513 878 219 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2420 2452 2420 Size: 32 6578 AlwNI CAG_nnn'CTG Cuts at: 2056 3184 4353 5925 2056 Size: 1128 1169 1572 2741 Fragments arranged by size: 2741 1572 1169 1128 ApaLI G'TGCA_C Cuts at: 3083 4667 5823 3083 Size: 1584 1156 3870 Fragments arranged by size: 3870 1584 1156 ApoI r'AATT_y Cuts at: 1493 2276 3919 4145 4297 6406 1493 Size: 783 1643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 783 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2161 3935 1870 Size: 73 67 151 1774 4545 Fragments arranged by size: 4545 1774 151 73 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2310 2343 2310 Size: 33 6577 BamHI G'GATC_C Cuts at: 3940 4307 3940 Size: 367 6243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2393 2488 2846 2884 Size: 325 217 627 396 210 95 358 38 Cuts at: 2884 3100 3113 3880 3931 5386 618 Size: 216 13 767 51 1455 1842 Fragments arranged by size: 1842 1455 767 627 396 358 325 217 216 210 95 51 38 13 BanII G_rGCy'C Cuts at: 1391 2162 2306 2569 3060 3132 3314 3595 3929 Size: 771 144 263 491 72 182 281 334 Cuts at: 3929 1391 Size: 4072 Fragments arranged by size: 4072 771 491 334 281 263 182 144 72 BbeI G_GCGC'C Cuts at: 2397 2397 Size: 6610 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3246 3621 928 Size: 971 1347 375 3917 Fragments arranged by size: 3917 1347 971 375 BbvCI CC'TCA_GC Cuts at: 3466 3466 Size: 6610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2747 2781 3590 3624 4801 4835 1903 Size: 34 810 34 809 34 1177 34 3678 Fragments arranged by size: 3678 1177 810 809 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2338 4502 5712 2338 Size: 2164 1210 3236 Fragments arranged by size: 3236 2164 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2243 2402 3146 Size: 107 122 71 631 605 571 159 744 Cuts at: 3146 5221 136 Size: 2075 1525 Fragments arranged by size: 2075 1525 744 631 605 571 159 122 107 71 BglII A'GATC_T Cuts at: 6605 6605 Size: 6610 BlpI GC'TnA_GC Cuts at: 3005 3572 4027 3005 Size: 567 455 5588 Fragments arranged by size: 5588 567 455 Bme1580I G_kGCm'C Cuts at: 1790 2287 2493 2889 3087 3116 3344 4671 5827 Size: 497 206 396 198 29 228 1327 1156 Cuts at: 5827 1790 Size: 2573 Fragments arranged by size: 2573 1327 1156 497 396 228 206 198 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6610 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3535 4341 5299 Size: 416 53 83 399 24 2118 806 958 Cuts at: 5299 442 Size: 1753 Fragments arranged by size: 2118 1753 958 806 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2034 1056 Size: 978 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2434 2689 2758 3185 3447 3863 5270 Size: 857 338 255 69 427 262 416 1407 Cuts at: 5270 1239 Size: 2579 Fragments arranged by size: 2579 1407 857 427 416 338 262 255 69 Bpu10I CC'TnA_GC Cuts at: 3363 3466 3656 3363 Size: 103 190 6317 Fragments arranged by size: 6317 190 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4735 5600 5898 4735 Size: 865 298 5447 Fragments arranged by size: 5447 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 2459 5273 882 Size: 617 672 288 2814 2219 Fragments arranged by size: 2814 2219 672 617 288 BsaAI yAC'GTr Cuts at: 493 1204 2173 2288 2326 2473 3820 4401 493 Size: 711 969 115 38 147 1347 581 2702 Fragments arranged by size: 2702 1347 969 711 581 147 115 38 BsaBI GATnn'nnATC Cuts at: 6604 6604 Size: 6610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2096 2394 2712 2760 Size: 53 83 186 936 563 298 318 48 Cuts at: 2760 4417 4799 5434 275 Size: 1657 382 635 1451 Fragments arranged by size: 1657 1451 936 635 563 382 318 298 186 83 53 48 BsaWI w'CCGG_w Cuts at: 5043 5441 5715 5862 6273 5043 Size: 398 274 147 411 5380 Fragments arranged by size: 5380 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2556 2586 3420 3450 1178 Size: 30 165 30 1153 30 834 30 4338 Fragments arranged by size: 4338 1153 834 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2795 3002 3122 3860 1186 Size: 1177 432 207 120 738 3936 Fragments arranged by size: 3936 1177 738 432 207 120 BseYI C'CCAG_C Cuts at: 2655 3263 3277 3872 5813 6309 2655 Size: 608 14 595 1941 496 2956 Fragments arranged by size: 2956 1941 608 595 496 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3497 3875 1163 Size: 573 1761 378 3898 Fragments arranged by size: 3898 1761 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4060 4821 5849 1540 Size: 275 186 2059 761 1028 2301 Fragments arranged by size: 2301 2059 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2178 2220 3087 3314 4671 4756 5827 2178 Size: 42 867 227 1357 85 1071 2961 Fragments arranged by size: 2961 1357 1071 867 227 85 42 BsmI GAATG_Cn' Cuts at: 4121 4214 4121 Size: 93 6517 BsmBI CGTCTCn'nnnn_ Cuts at: 2087 2087 Size: 6610 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2178 2220 2287 2306 2493 2569 Size: 399 372 16 42 67 19 187 76 Cuts at: 2569 2889 3060 3087 3116 3132 3314 3344 3595 Size: 320 171 27 29 16 182 30 251 Cuts at: 3595 3929 4671 4756 5827 1391 Size: 334 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 334 320 251 187 182 171 85 76 67 42 30 29 27 19 16 16 BspEI T'CCGG_A Cuts at: 6273 6273 Size: 6610 BspHI T'CATG_A Cuts at: 4499 6229 4499 Size: 1730 4880 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2476 3965 844 Size: 1632 1489 3489 Fragments arranged by size: 3489 1632 1489 BsrBI CCG'CTC Cuts at: 1394 1823 4497 1394 Size: 429 2674 3507 Fragments arranged by size: 3507 2674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2025 2763 2835 5105 5279 65 Size: 1183 777 738 72 2270 174 1396 Fragments arranged by size: 2270 1396 1183 777 738 174 72 BsrFI r'CCGG_y Cuts at: 1700 1810 2720 5254 5441 1700 Size: 110 910 2534 187 2869 Fragments arranged by size: 2869 2534 910 187 110 BsrGI T'GTAC_A Cuts at: 96 3346 96 Size: 3250 3360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6610 BssSI C'ACGA_G Cuts at: 4670 5682 4670 Size: 1012 5598 BstAPI GCAn_nnn'nTGC Cuts at: 794 2846 3862 794 Size: 2052 1016 3542 Fragments arranged by size: 3542 2052 1016 BstBI TT'CG_AA Cuts at: 5414 5414 Size: 6610 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6610 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2200 Size: 6610 BstYI r'GATC_y Cuts at: 1972 3627 3940 4307 4692 4709 6161 6247 6259 Size: 1655 313 367 385 17 1452 86 12 Cuts at: 6259 6605 1972 Size: 346 1977 Fragments arranged by size: 1977 1655 1452 385 367 346 313 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3389 3389 Size: 6610 BtgI C'CryG_G Cuts at: 513 1065 2004 2187 2331 2400 2795 3042 3633 Size: 552 939 183 144 69 395 247 591 Cuts at: 3633 513 Size: 3490 Fragments arranged by size: 3490 939 591 552 395 247 183 144 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2803 487 Size: 683 56 1577 4294 Fragments arranged by size: 4294 1577 683 56 BtsI GCAGTG_nn' Cuts at: 2099 2724 3149 3438 3750 4126 4920 4940 2099 Size: 625 425 289 312 376 794 20 3769 Fragments arranged by size: 3769 794 625 425 376 312 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6575 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DraIII CAC_nnn'GTG Cuts at: 3820 3820 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5617 809 Size: 507 4301 1802 Fragments arranged by size: 4301 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2235 2601 Size: 54 1425 50 275 18 360 45 366 Cuts at: 2601 2718 2814 3045 3219 3898 4057 4946 6575 Size: 117 96 231 174 679 159 889 1629 Cuts at: 6575 8 Size: 43 Fragments arranged by size: 1629 1425 889 679 366 360 275 231 174 159 117 96 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4057 1537 Size: 275 2245 4090 Fragments arranged by size: 4090 2245 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2630 3020 3065 4540 1353 Size: 786 491 390 45 1475 3423 Fragments arranged by size: 3423 1475 786 491 390 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5197 5571 5717 387 Size: 1064 138 3608 374 146 1280 Fragments arranged by size: 3608 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3312 3312 Size: 6610 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2096 2434 2689 2758 3185 3398 Size: 393 455 9 338 255 69 427 213 Cuts at: 3398 3447 3863 4673 5270 6057 6325 1239 Size: 49 416 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 455 427 416 393 338 268 255 213 69 49 9 EcoNI CCTnn'n_nnAGG Cuts at: 3366 3366 Size: 6610 EcoO109I rG'GnC_Cy Cuts at: 3393 3892 3393 Size: 499 6111 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FspI TGC'GCA Cuts at: 5116 5116 Size: 6610 HaeII r_GCGC'y Cuts at: 1678 2110 2397 2676 2918 3449 5757 1678 Size: 432 287 279 242 531 2308 2531 Fragments arranged by size: 2531 2308 531 432 287 279 242 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2894 2926 3152 3184 5251 Size: 32 135 32 1322 32 226 32 2067 Cuts at: 5251 5283 5325 5357 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2067 1322 226 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3954 4200 669 Size: 988 281 2016 246 3079 Fragments arranged by size: 3079 2016 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6610 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KasI G'GCGC_C Cuts at: 2393 2393 Size: 6610 KpnI G_GTAC'C Cuts at: 3935 3935 Size: 6610 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5724 5908 1305 Size: 4419 184 2007 Fragments arranged by size: 4419 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 2816 3047 3221 3900 Size: 54 1425 343 360 624 231 174 679 Cuts at: 3900 10 Size: 2720 Fragments arranged by size: 2720 1425 679 624 360 343 231 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2044 3215 4568 4927 5086 518 Size: 648 878 1171 1353 359 159 2042 Fragments arranged by size: 2042 1353 1171 878 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2240 2843 2938 2972 3334 3787 4026 Size: 468 166 603 95 34 362 453 239 Cuts at: 4026 4703 5851 6096 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 603 468 453 362 245 239 166 95 34 NaeI GCC'GGC Cuts at: 2722 2722 Size: 6610 NarI GG'CG_CC Cuts at: 2394 2394 Size: 6610 NcoI C'CATG_G Cuts at: 513 1065 2004 2331 3042 3633 513 Size: 552 939 327 711 591 3490 Fragments arranged by size: 3490 939 711 591 552 327 NdeI CA'TA_TG Cuts at: 387 3903 387 Size: 3516 3094 NgoMIV G'CCGG_C Cuts at: 2720 2720 Size: 6610 NheI G'CTAG_C Cuts at: 1052 2030 1052 Size: 978 5632 NotI GC'GGCC_GC Cuts at: 4057 4057 Size: 6610 NspI r_CATG'y Cuts at: 1132 1229 3968 5513 1132 Size: 97 2739 1545 2229 Fragments arranged by size: 2739 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3831 1348 Size: 2483 4127 PciI A'CATG_T Cuts at: 5509 5509 Size: 6610 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6610 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PmlI CAC'GTG Cuts at: 2288 2473 2288 Size: 185 6425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4679 4711 6192 6224 4679 Size: 32 1481 32 5065 Fragments arranged by size: 5065 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3892 3892 Size: 6610 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6610 PsiI TTA'TAA Cuts at: 4180 4180 Size: 6610 PstI C_TGCA'G Cuts at: 830 3962 830 Size: 3132 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 2843 2972 3334 2843 Size: 129 362 6119 Fragments arranged by size: 6119 362 129 SacI G_AGCT'C Cuts at: 3314 3314 Size: 6610 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SapI GCTCTTCn'nnn_ Cuts at: 3065 3065 Size: 6610 SbfI CC_TGCA'GG Cuts at: 3962 3962 Size: 6610 ScaI AGT'ACT Cuts at: 4858 6570 4858 Size: 1712 4898 SfcI C'TryA_G Cuts at: 826 1045 2944 3329 3958 5093 5774 5965 826 Size: 219 1899 385 629 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 629 385 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2243 2243 Size: 6610 SfoI GGC'GCC Cuts at: 2395 2395 Size: 6610 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6610 SmaI CCC'GGG Cuts at: 1872 2012 3937 1872 Size: 140 1925 4545 Fragments arranged by size: 4545 1925 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 4714 5615 5877 820 Size: 197 926 2771 901 262 1553 Fragments arranged by size: 2771 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2326 4401 493 Size: 1833 2075 2702 Fragments arranged by size: 2702 2075 1833 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 3968 1132 Size: 2836 3774 SspI AAT'ATT Cuts at: 5 52 4534 5 Size: 47 4482 2081 Fragments arranged by size: 4482 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6610 StyI C'CwwG_G Cuts at: 513 1065 2004 2193 2331 3042 3190 3633 4049 Size: 552 939 189 138 711 148 443 416 Cuts at: 4049 513 Size: 3074 Fragments arranged by size: 3074 939 711 552 443 416 189 148 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 3697 4637 4654 4807 4992 5437 Size: 1255 555 1562 940 17 153 185 445 Cuts at: 5437 325 Size: 1498 Fragments arranged by size: 1562 1498 1255 940 555 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3346 4856 6568 Size: 274 80 33 51 1443 1369 1510 1712 Cuts at: 6568 96 Size: 138 Fragments arranged by size: 1712 1510 1443 1369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2556 2588 3420 3452 3729 3761 6570 6602 2556 Size: 32 832 32 277 32 2809 32 2564 Fragments arranged by size: 2809 2564 832 277 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6610 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2032 3817 3838 2032 Size: 1785 21 4804 Fragments arranged by size: 4804 1785 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6610 XmaI C'CCGG_G Cuts at: 1870 2010 3935 1870 Size: 140 1925 4545 Fragments arranged by size: 4545 1925 140 XmnI GAAnn'nnTTC Cuts at: 4739 4739 Size: 6610 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2097 2761 4418 276 Size: 53 83 186 936 563 664 1657 2468 Fragments arranged by size: 2468 1657 936 664 563 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI