Restriction map for
pFN21AE0346
(Linear) (Six-base) MAPSORT of: KIEE0346.seq Check: 7980 from: 1 to: 1905
KIEE0346.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 471 1905
Size: 471 1434
AatII G_ACGT'C
Cuts at: 0 94 758 1905
Size: 94 664 1147
Fragments arranged by size:
1147 664 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1393 1905
Size: 82 1311 512
Fragments arranged by size:
1311 512 82
AfeI AGC'GCT
Cuts at: 0 669 911 1905
Size: 669 242 994
Fragments arranged by size:
994 669 242
AflIII A'CryG_T
Cuts at: 0 282 1905
Size: 282 1623
AhdI GACnn_n'nnGTC
Cuts at: 0 1161 1905
Size: 1161 744
AleI CACnn'nnGTG
Cuts at: 0 1210 1905
Size: 1210 695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 57 276 1905
Size: 57 219 1629
Fragments arranged by size:
1629 219 57
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 1905
Size: 415 32 1458
Fragments arranged by size:
1458 415 32
AlwNI CAG_nnn'CTG
Cuts at: 0 51 1179 1905
Size: 51 1128 726
Fragments arranged by size:
1128 726 51
ApaLI G'TGCA_C
Cuts at: 0 1078 1905
Size: 1078 827
ApoI r'AATT_y
Cuts at: 0 271 1905
Size: 271 1634
AvaI C'yCGr_G
Cuts at: 0 5 156 1905
Size: 5 151 1749
Fragments arranged by size:
1749 151 5
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 305 338 1905
Size: 305 33 1567
Fragments arranged by size:
1567 305 33
BanI G'GyrC_C
Cuts at: 0 178 388 483 841 879 1095 1108 1875
Size: 178 210 95 358 38 216 13 767
Cuts at: 1875 1905
Size: 30
Fragments arranged by size:
767 358 216 210 178 95 38 30
13
BanII G_rGCy'C
Cuts at: 0 157 301 564 1055 1127 1309 1590 1905
Size: 157 144 263 491 72 182 281 315
Fragments arranged by size:
491 315 281 263 182 157 144 72
BbeI G_GCGC'C
Cuts at: 0 392 1905
Size: 392 1513
BbsI GAAGACnn'nnnn_
Cuts at: 0 1241 1616 1905
Size: 1241 375 289
Fragments arranged by size:
1241 375 289
BbvCI CC'TCA_GC
Cuts at: 0 1461 1905
Size: 1461 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 742 776 1585 1619 1905
Size: 742 34 809 34 286
Fragments arranged by size:
809 742 286 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 333 1905
Size: 333 1572
BglI GCCn_nnn'nGGC
Cuts at: 0 238 397 1141 1905
Size: 238 159 744 764
Fragments arranged by size:
764 744 238 159
BlpI GC'TnA_GC
Cuts at: 0 1000 1567 1905
Size: 1000 567 338
Fragments arranged by size:
1000 567 338
Bme1580I G_kGCm'C
Cuts at: 0 282 488 884 1082 1111 1339 1905
Size: 282 206 396 198 29 228 566
Fragments arranged by size:
566 396 282 228 206 198 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1530 1905
Size: 1530 375
BmtI G_CTAG'C
Cuts at: 0 29 1905
Size: 29 1876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 429 684 753 1180 1442 1858 1905
Size: 91 338 255 69 427 262 416 47
Fragments arranged by size:
427 416 338 262 255 91 69 47
Bpu10I CC'TnA_GC
Cuts at: 0 1358 1461 1651 1905
Size: 1358 103 190 254
Fragments arranged by size:
1358 254 190 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 454 1905
Size: 166 288 1451
Fragments arranged by size:
1451 288 166
BsaAI yAC'GTr
Cuts at: 0 168 283 321 468 1815 1905
Size: 168 115 38 147 1347 90
Fragments arranged by size:
1347 168 147 115 90 38
BsaHI Gr'CG_yC
Cuts at: 0 91 389 707 755 1905
Size: 91 298 318 48 1150
Fragments arranged by size:
1150 318 298 91 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 551 581 1415 1445 1905
Size: 551 30 834 30 460
Fragments arranged by size:
834 551 460 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 790 997 1117 1855 1905
Size: 358 432 207 120 738 50
Fragments arranged by size:
738 432 358 207 120 50
BseYI C'CCAG_C
Cuts at: 0 650 1258 1272 1867 1905
Size: 650 608 14 595 38
Fragments arranged by size:
650 608 595 38 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1492 1870 1905
Size: 1492 378 35
Fragments arranged by size:
1492 378 35
BsiHKAI G_wGCw'C
Cuts at: 0 173 215 1082 1309 1905
Size: 173 42 867 227 596
Fragments arranged by size:
867 596 227 173 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 82 1905
Size: 82 1823
Bsp1286I G_dGCh'C
Cuts at: 0 157 173 215 282 301 488 564 884
Size: 157 16 42 67 19 187 76 320
Cuts at: 884 1055 1082 1111 1127 1309 1339 1590 1905
Size: 171 27 29 16 182 30 251 315
Fragments arranged by size:
320 315 251 187 182 171 157 76
67 42 30 29 27 19 16 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 471 1905
Size: 471 1434
BsrDI GCAATG_nn'
Cuts at: 0 20 758 830 1905
Size: 20 738 72 1075
Fragments arranged by size:
1075 738 72 20
BsrFI r'CCGG_y
Cuts at: 0 715 1905
Size: 715 1190
BsrGI T'GTAC_A
Cuts at: 0 1341 1905
Size: 1341 564
BstAPI GCAn_nnn'nTGC
Cuts at: 0 841 1857 1905
Size: 841 1016 48
Fragments arranged by size:
1016 841 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1905
Size: 195 1710
BstYI r'GATC_y
Cuts at: 0 1622 1905
Size: 1622 283
Bsu36I CC'TnA_GG
Cuts at: 0 1384 1905
Size: 1384 521
BtgI C'CryG_G
Cuts at: 0 182 326 395 790 1037 1628 1905
Size: 182 144 69 395 247 591 277
Fragments arranged by size:
591 395 277 247 182 144 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 798 1905
Size: 798 1107
BtsI GCAGTG_nn'
Cuts at: 0 94 719 1144 1433 1745 1905
Size: 94 625 425 289 312 160
Fragments arranged by size:
625 425 312 289 160 94
DraIII CAC_nnn'GTG
Cuts at: 0 1815 1905
Size: 1815 90
EaeI y'GGCC_r
Cuts at: 0 185 230 596 713 809 1040 1214 1893
Size: 185 45 366 117 96 231 174 679
Cuts at: 1893 1905
Size: 12
Fragments arranged by size:
679 366 231 185 174 117 96 45
12
EarI CTCTTCn'nnn_
Cuts at: 0 134 625 1015 1060 1905
Size: 134 491 390 45 845
Fragments arranged by size:
845 491 390 134 45
EcoICRI GAG'CTC
Cuts at: 0 1307 1905
Size: 1307 598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 91 429 684 753 1180 1393 1442
Size: 82 9 338 255 69 427 213 49
Cuts at: 1442 1858 1905
Size: 416 47
Fragments arranged by size:
427 416 338 255 213 82 69 49
47 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1361 1905
Size: 1361 544
EcoO109I rG'GnC_Cy
Cuts at: 0 1388 1887 1905
Size: 1388 499 18
Fragments arranged by size:
1388 499 18
HaeII r_GCGC'y
Cuts at: 0 105 392 671 913 1444 1905
Size: 105 287 279 242 531 461
Fragments arranged by size:
531 461 287 279 242 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 889 921 1147 1179 1905
Size: 889 32 226 32 726
Fragments arranged by size:
889 726 226 32 32
KasI G'GCGC_C
Cuts at: 0 388 1905
Size: 388 1517
MscI TGG'CCA
Cuts at: 0 187 811 1042 1216 1895 1905
Size: 187 624 231 174 679 10
Fragments arranged by size:
679 624 231 187 174 10
MslI CAynn'nnrTG
Cuts at: 0 39 1210 1905
Size: 39 1171 695
Fragments arranged by size:
1171 695 39
MspA1I CmG'CkG
Cuts at: 0 69 235 838 933 967 1329 1782 1905
Size: 69 166 603 95 34 362 453 123
Fragments arranged by size:
603 453 362 166 123 95 69 34
NaeI GCC'GGC
Cuts at: 0 717 1905
Size: 717 1188
NarI GG'CG_CC
Cuts at: 0 389 1905
Size: 389 1516
NcoI C'CATG_G
Cuts at: 0 326 1037 1628 1905
Size: 326 711 591 277
Fragments arranged by size:
711 591 326 277
NdeI CA'TA_TG
Cuts at: 0 1898 1905
Size: 1898 7
NgoMIV G'CCGG_C
Cuts at: 0 715 1905
Size: 715 1190
NheI G'CTAG_C
Cuts at: 0 25 1905
Size: 25 1880
PasI CC'CwG_GG
Cuts at: 0 1826 1905
Size: 1826 79
PmlI CAC'GTG
Cuts at: 0 283 468 1905
Size: 283 185 1437
Fragments arranged by size:
1437 283 185
PpuMI rG'GwC_Cy
Cuts at: 0 1887 1905
Size: 1887 18
PvuII CAG'CTG
Cuts at: 0 838 967 1329 1905
Size: 838 129 362 576
Fragments arranged by size:
838 576 362 129
SacI G_AGCT'C
Cuts at: 0 1309 1905
Size: 1309 596
SapI GCTCTTCn'nnn_
Cuts at: 0 1060 1905
Size: 1060 845
SfcI C'TryA_G
Cuts at: 0 939 1324 1905
Size: 939 385 581
Fragments arranged by size:
939 581 385
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 238 1905
Size: 238 1667
SfoI GGC'GCC
Cuts at: 0 390 1905
Size: 390 1515
SmaI CCC'GGG
Cuts at: 0 7 1905
Size: 7 1898
SnaBI TAC'GTA
Cuts at: 0 321 1905
Size: 321 1584
StyI C'CwwG_G
Cuts at: 0 188 326 1037 1185 1628 1905
Size: 188 138 711 148 443 277
Fragments arranged by size:
711 443 277 188 148 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 1692 1905
Size: 130 1562 213
Fragments arranged by size:
1562 213 130
TatI w'GTAC_w
Cuts at: 0 1341 1905
Size: 1341 564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1415 1447 1724 1756 1905
Size: 551 32 832 32 277 32 149
Fragments arranged by size:
832 551 277 149 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 27 1812 1833 1905
Size: 27 1785 21 72
Fragments arranged by size:
1785 72 27 21
XmaI C'CCGG_G
Cuts at: 0 5 1905
Size: 5 1900
ZraI GAC'GTC
Cuts at: 0 92 756 1905
Size: 92 664 1149
Fragments arranged by size:
1149 664 92
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI PasI PmlI PpuMI PvuII
SacI SapI SfcI SfiI SfoI SmaI SnaBI StyI
TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI ApaI AscI AseI
AsiSI AvrII BamHI BclI BfrBI BglII BmgBI BplI
BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SgfI SgrAI SmlI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmnI