Restriction map for
pFN21AE0325
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1087 GACGTC GACGT^C
AlwNI 1180 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 108 GGGCCC GGGCC^C
AspHI 925 GAGCTC G[AT]GC[AT]^C
AvaI 901 CCCGAG C^[CT]CG[GA]G
BbeI 6 GGCGCC GGCGC^C
BbsI 192 GAAGAC GAAGAC(2/6)
BbvCI 222 CCTCAGC CCTCAGC(-5/-2)
BcgI 105 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 192 GAAGAC GAAGAC(2/6)
BseRI 535 GAGGAG GAGGAG(10/8)
BsiHKAI 925 GAGCTC G[AT]GC[AT]^C
BsmAI 586 GTCTC GTCTC(1/5)
BsoBI 901 CCCGAG C^[CT]CG[GA]G
BsrDI 227 GCAATG GCAATG(2/0)
BsrFI 1493 GCCGGC [GA]^CCGG[CT]
BstYI 1390 AGATCC [GA]^GATC[CT]
Cfr10I 1493 GCCGGC [GA]^CCGG[CT]
EarI 1012 CTCTTC CTCTTC(1/4)
HaeII 6 GGCGCC [GA]GCGC^[CT]
HincII 306 GTTGAC GT[CT]^[GA]AC
HindII 306 GTTGAC GT[CT]^[GA]AC
KasI 6 GGCGCC G^GCGCC
Ksp632I 1012 CTCTTC CTCTTC(1/4)
MflI 1390 AGATCC [GA]^GATC[CT]
MslI 78 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1493 GCCGGC GCC^GGC
NarI 6 GGCGCC GG^CGCC
NgoMIV 1493 GCCGGC G^CCGGC
NspI 1218 GCATGT [GA]CATG^[CT]
SacI 925 GAGCTC GAGCT^C
SexAI 572 ACCTGGT A^CC[AT]GGT
SfoI 6 GGCGCC GGC^GCC
TfiI 1361 GAATC G^A[AT]TC
Tsp509I 684 AATT ^AATT
XcmI 866 CCATCCCCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1390 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 1108 CGCG CG^CG
1168 CGCG
AcyI 6 GGCGCC G[GA]^CG[CT]C
1087 GACGTC
AlwI 1095 GATCC GGATC(4/5)
1391 GATCC
BalI 272 TGGCCA TGG^CCA
440 TGGCCA
BanII 108 GGGCCC G[GA]GC[CT]^C
925 GAGCTC
BbrPI 709 CACGTG CAC^GTG
763 CACGTG
BcnI 69 CCCGG CC^[GC]GG
1158 CCCGG
BsaAI 709 CACGTG [CT]AC^GT[GA]
763 CACGTG
BsaHI 6 GGCGCC G[GA]^CG[CT]C
1087 GACGTC
Bsp1407I 482 TGTACA T^GTACA
986 TGTACA
BsrGI 482 TGTACA T^GTACA
986 TGTACA
BsrI 551 ACTGG ACTGG(1/-1)
1475 ACTGG
BstUI 1108 CGCG CG^CG
1168 CGCG
BstXI 1277 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1400 CCAAAATCCTGG
EcoT14I 83 CCATGG C^C[AT][AT]GG
269 CCATGG
Hin1I 6 GGCGCC G[GA]^CG[CT]C
1087 GACGTC
MaeIII 592 GTCAC ^GT[ACGT]AC
1129 GTCAC
MluNI 272 TGGCCA TGG^CCA
440 TGGCCA
MscI 272 TGGCCA TGG^CCA
440 TGGCCA
MvnI 1108 CGCG CG^CG
1168 CGCG
NciI 69 CCCGG CC^[GC]GG
1158 CCCGG
NcoI 83 CCATGG C^CATGG
269 CCATGG
PleI 257 GACTC GAGTC(4/5)
1045 GAGTC
PmaCI 709 CACGTG CAC^GTG
763 CACGTG
PmlI 709 CACGTG CAC^GTG
763 CACGTG
PpuMI 412 GGGACCC [GA]G^G[AT]CC[CT]
932 AGGACCC
PshAI 309 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1122 GACAGCAGTC
PstI 13 CTGCAG CTGCA^G
793 CTGCAG
SspBI 482 TGTACA T^GTACA
986 TGTACA
StyI 83 CCATGG C^C[AT][AT]GG
269 CCATGG
Tsp45I 592 GTCAC ^GT[GC]AC
1129 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AocI AvaII
BanI BbvI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TseI TspRI TthHB8I