(Circular) (Six-base) MAPSORT of: pFN21AE0325.seq Check: 8575 from: 1 to: 6217 pFN21AE0325 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2358 2358 Size: 6217 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3096 4027 278 Size: 53 83 186 936 1560 931 2468 Fragments arranged by size: 2468 1560 936 931 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2746 2802 4280 5664 5932 1632 Size: 1114 56 1478 1384 268 1917 Fragments arranged by size: 1917 1478 1384 1114 268 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 3190 3960 5532 3190 Size: 770 1572 3875 Fragments arranged by size: 3875 1572 770 ApaI G_GGCC'C Cuts at: 2117 2117 Size: 6217 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2906 3542 1870 Size: 73 963 636 4545 Fragments arranged by size: 4545 963 636 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2041 3311 3538 4993 Size: 325 217 627 224 30 1270 227 1455 Cuts at: 4993 618 Size: 1842 Fragments arranged by size: 1842 1455 1270 627 325 227 224 217 30 BanII G_rGCy'C Cuts at: 1391 2117 2934 3536 1391 Size: 726 817 602 4072 Fragments arranged by size: 4072 817 726 602 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 6217 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2204 928 Size: 971 305 4941 Fragments arranged by size: 4941 971 305 BbvCI CC'TCA_GC Cuts at: 2228 2228 Size: 6217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2099 2133 4408 4442 1903 Size: 34 162 34 2275 34 3678 Fragments arranged by size: 3678 2275 162 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4828 136 Size: 107 122 71 631 605 337 2819 1525 Fragments arranged by size: 2819 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 2046 2117 2721 3150 4278 5434 1790 Size: 256 71 604 429 1128 1156 2573 Fragments arranged by size: 2573 1156 1128 604 429 256 71 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3490 3948 4906 Size: 416 53 83 399 24 2073 458 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 2073 1753 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2317 2441 2558 2635 4877 1239 Size: 1078 124 117 77 2242 2579 Fragments arranged by size: 2579 2242 1078 124 117 77 Bpu10I CC'TnA_GC Cuts at: 2228 2228 Size: 6217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 2918 3481 4342 5207 5505 2342 Size: 576 563 861 865 298 3054 Fragments arranged by size: 3054 865 861 576 563 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2716 2770 4008 493 Size: 711 1512 54 1238 2702 Fragments arranged by size: 2702 1512 1238 711 54 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3093 4024 4406 Size: 53 83 186 936 479 1081 931 382 Cuts at: 4406 5041 275 Size: 635 1451 Fragments arranged by size: 1451 1081 936 931 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2555 1186 Size: 1369 4848 BseYI C'CCAG_C Cuts at: 2162 2398 2834 5420 5916 2162 Size: 236 436 2586 496 2463 Fragments arranged by size: 2586 2463 496 436 236 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2103 2812 2813 3047 1163 Size: 573 367 709 1 234 4333 Fragments arranged by size: 4333 709 573 367 234 1 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2934 4278 4363 5434 2934 Size: 1344 85 1071 3717 Fragments arranged by size: 3717 1344 1071 85 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2117 2721 2934 3150 3536 4278 Size: 399 256 71 604 213 216 386 742 Cuts at: 4278 4363 5434 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 386 256 216 213 85 71 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2791 3270 3572 844 Size: 1514 433 479 302 3489 Fragments arranged by size: 3489 1514 479 433 302 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2239 4712 4886 65 Size: 1183 991 2473 174 1396 Fragments arranged by size: 2473 1396 1183 991 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3498 4861 5048 1700 Size: 110 1688 1363 187 2869 Fragments arranged by size: 2869 1688 1363 187 110 BsrGI T'GTAC_A Cuts at: 96 2487 2991 96 Size: 2391 504 3322 Fragments arranged by size: 3322 2391 504 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 3289 3412 3289 Size: 123 6094 BstYI r'GATC_y Cuts at: 1972 3395 3547 3914 4299 4316 5768 5854 5866 Size: 1423 152 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1423 385 367 346 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2365 2811 3487 2365 Size: 446 676 5095 Fragments arranged by size: 5095 676 446 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2274 3375 513 Size: 552 939 84 186 1101 3355 Fragments arranged by size: 3355 1101 939 552 186 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2965 487 Size: 683 56 1739 3739 Fragments arranged by size: 3739 1739 683 56 BtsI GCAGTG_nn' Cuts at: 2726 3733 4527 4547 2726 Size: 1007 794 20 4396 Fragments arranged by size: 4396 1007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2445 3117 Size: 54 1425 50 275 18 447 168 672 Cuts at: 3117 3664 4553 6182 8 Size: 547 889 1629 43 Fragments arranged by size: 1629 1425 889 672 547 447 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3023 4147 1353 Size: 1670 1124 3423 Fragments arranged by size: 3423 1670 1124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2932 2932 Size: 6217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2441 2558 2635 2746 2802 4280 Size: 393 685 124 117 77 111 56 1478 Cuts at: 4280 4877 5664 5932 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1478 787 685 597 393 268 124 117 111 77 56 EcoO109I rG'GnC_Cy Cuts at: 2113 2114 2418 2938 2113 Size: 1 304 520 5392 Fragments arranged by size: 5392 520 304 1 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2015 5364 1678 Size: 337 3349 2531 Fragments arranged by size: 3349 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3470 3502 4858 4890 4932 Size: 32 135 32 1898 32 1356 32 42 Cuts at: 4932 4964 1373 Size: 32 2626 Fragments arranged by size: 2626 1898 1356 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2313 3561 3807 669 Size: 988 281 375 1248 246 3079 Fragments arranged by size: 3079 1248 988 375 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2011 2011 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2994 3033 5331 5515 1305 Size: 1689 39 2298 184 2007 Fragments arranged by size: 2298 2007 1689 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 2447 10 Size: 54 1425 343 447 168 3780 Fragments arranged by size: 3780 1425 447 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 4175 4534 4693 518 Size: 648 921 2088 359 159 2042 Fragments arranged by size: 2088 2042 921 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2250 2318 2398 2655 2797 3213 3633 4310 Size: 644 68 80 257 142 416 420 677 Cuts at: 4310 5458 5703 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 644 420 416 257 245 142 80 68 NaeI GCC'GGC Cuts at: 3500 3500 Size: 6217 NarI GG'CG_CC Cuts at: 2012 2012 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2004 2088 2274 513 Size: 552 939 84 186 4456 Fragments arranged by size: 4456 939 552 186 84 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 3498 3498 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3227 3575 5120 1132 Size: 97 1998 348 1545 2229 Fragments arranged by size: 2229 1998 1545 348 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PmlI CAC'GTG Cuts at: 2716 2770 2716 Size: 54 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2418 2938 2418 Size: 520 5697 PshAI GACnn'nnGTC Cuts at: 1613 2318 3131 1613 Size: 705 813 4699 Fragments arranged by size: 4699 813 705 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2113 2113 Size: 6217 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2558 2590 2558 Size: 32 6185 PstI C_TGCA'G Cuts at: 830 2022 2802 3569 830 Size: 1192 780 767 3478 Fragments arranged by size: 3478 1192 780 767 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2318 2398 2797 3213 2318 Size: 80 399 416 5322 Fragments arranged by size: 5322 416 399 80 SacI G_AGCT'C Cuts at: 2934 2934 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SanDI GG'GwC_CC Cuts at: 2418 2418 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SexAI A'CCwGG_T Cuts at: 2577 2577 Size: 6217 SfcI C'TryA_G Cuts at: 826 1045 2018 2150 2798 3070 3565 4700 5381 Size: 219 973 132 648 272 495 1135 681 Cuts at: 5381 5572 826 Size: 191 1471 Fragments arranged by size: 1471 1135 973 681 648 495 272 219 191 132 SfoI GGC'GCC Cuts at: 2013 2013 Size: 6217 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2357 2933 3460 4321 5222 5484 Size: 197 926 414 576 527 861 901 262 Cuts at: 5484 820 Size: 1553 Fragments arranged by size: 1553 926 901 861 576 527 414 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2274 3656 513 Size: 552 939 84 186 1382 3074 Fragments arranged by size: 3074 1382 939 552 186 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 4261 4414 4599 5044 325 Size: 1255 2664 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 2991 3000 Size: 274 80 33 51 1443 510 504 9 Cuts at: 3000 4463 6175 96 Size: 1463 1712 138 Fragments arranged by size: 1712 1463 1443 510 504 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2878 2878 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3094 4025 276 Size: 53 83 186 936 1560 931 2468 Fragments arranged by size: 2468 1560 936 931 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI RsrII SacII SapI SfiI SrfI SwaI