(Circular) (Six-base) MAPSORT of: pFN21AE0364.seq Check: 6361 from: 1 to: 6775 pFN21AE0364 6775 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3712 3712 Size: 6775 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3297 4118 1937 Size: 1360 821 4594 Fragments arranged by size: 4594 1360 821 Acc65I G'GTAC_C Cuts at: 4096 4096 Size: 6775 AclI AA'CG_TT Cuts at: 1563 4902 5275 1563 Size: 3339 373 3063 Fragments arranged by size: 3339 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 2501 4838 6222 6490 1632 Size: 698 171 2337 1384 268 1917 Fragments arranged by size: 2337 1917 1384 698 268 171 AfeI AGC'GCT Cuts at: 3269 3269 Size: 6775 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6578 AflIII A'CryG_T Cuts at: 1203 2126 3687 3747 5674 6651 1203 Size: 923 1561 60 1927 977 1327 Fragments arranged by size: 1927 1561 1327 977 923 60 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6775 AlwNI CAG_nnn'CTG Cuts at: 2087 2834 4518 6090 2087 Size: 747 1684 1572 2772 Fragments arranged by size: 2772 1684 1572 747 ApaI G_GGCC'C Cuts at: 2479 2671 3355 4012 2479 Size: 192 684 657 5242 Fragments arranged by size: 5242 684 657 192 ApaLI G'TGCA_C Cuts at: 4832 5988 4832 Size: 1156 5619 ApoI r'AATT_y Cuts at: 1493 4084 4310 4462 6571 1493 Size: 2591 226 152 2109 1697 Fragments arranged by size: 2591 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5329 160 Size: 872 4297 1606 Fragments arranged by size: 4297 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 2454 2670 2890 3537 3656 4100 1870 Size: 73 511 216 220 647 119 444 4545 Fragments arranged by size: 4545 647 511 444 220 216 119 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3157 3190 3157 Size: 33 6742 BamHI G'GATC_C Cuts at: 2119 3039 4105 4472 2119 Size: 920 1066 367 4422 Fragments arranged by size: 4422 1066 920 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 4096 5551 618 Size: 325 217 627 395 1914 1455 1842 Fragments arranged by size: 1914 1842 1455 627 395 325 217 BanII G_rGCy'C Cuts at: 1391 2479 2663 2671 2794 3159 3355 4012 4094 Size: 1088 184 8 123 365 196 657 82 Cuts at: 4094 1391 Size: 4072 Fragments arranged by size: 4072 1088 657 365 196 184 123 82 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3944 928 Size: 971 2045 3759 Fragments arranged by size: 3759 2045 971 BbvCI CC'TCA_GC Cuts at: 2759 2759 Size: 6775 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4966 5000 1903 Size: 34 3029 34 3678 Fragments arranged by size: 3678 3029 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4667 5877 4667 Size: 1210 5565 BclI T'GATC_A Cuts at: 1548 1548 Size: 6775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 2191 2239 Size: 107 122 71 631 605 405 114 48 Cuts at: 2239 2465 2483 5386 136 Size: 226 18 2903 1525 Fragments arranged by size: 2903 1525 631 605 405 226 122 114 107 71 48 18 BglII A'GATC_T Cuts at: 3055 6770 3055 Size: 3715 3060 BlpI GC'TnA_GC Cuts at: 2195 2487 2657 4192 2195 Size: 292 170 1535 4778 Fragments arranged by size: 4778 1535 292 170 Bme1580I G_kGCm'C Cuts at: 1790 2187 2398 2479 2671 3355 4012 4836 5992 Size: 397 211 81 192 684 657 824 1156 Cuts at: 5992 1790 Size: 2573 Fragments arranged by size: 2573 1156 824 684 657 397 211 192 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2822 4006 4506 Size: 416 53 83 399 24 1405 1184 500 Cuts at: 4506 5464 442 Size: 958 1753 Fragments arranged by size: 1753 1405 1184 958 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3809 1056 Size: 2753 4022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 4087 5435 1239 Size: 1490 1358 1348 2579 Fragments arranged by size: 2579 1490 1358 1348 Bpu10I CC'TnA_GC Cuts at: 2759 2759 Size: 6775 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2347 4900 5765 6063 2347 Size: 2553 865 298 3059 Fragments arranged by size: 3059 2553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3248 5438 882 Size: 617 1749 2190 2219 Fragments arranged by size: 2219 2190 1749 617 BsaAI yAC'GTr Cuts at: 493 1204 3019 4566 493 Size: 711 1815 1547 2702 Fragments arranged by size: 2702 1815 1547 711 BsaBI GATnn'nnATC Cuts at: 6769 6769 Size: 6775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3903 4582 4964 5599 Size: 53 83 186 936 2370 679 382 635 Cuts at: 5599 275 Size: 1451 Fragments arranged by size: 2370 1451 936 679 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3198 5208 5606 5880 6027 6438 3198 Size: 2010 398 274 147 411 3535 Fragments arranged by size: 3535 2010 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2211 2241 3698 3728 3824 Size: 30 165 30 808 30 1457 30 96 Cuts at: 3824 3854 1178 Size: 30 4099 Fragments arranged by size: 4099 1457 808 165 96 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2726 3365 1186 Size: 1540 639 4596 Fragments arranged by size: 4596 1540 639 BseYI C'CCAG_C Cuts at: 2491 2578 2593 2849 3183 5978 6474 2491 Size: 87 15 256 334 2795 496 2792 Fragments arranged by size: 2795 2792 496 334 256 87 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3425 3821 1163 Size: 573 1689 396 4117 Fragments arranged by size: 4117 1689 573 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3652 4225 4986 6014 1540 Size: 275 186 1651 573 761 1028 2301 Fragments arranged by size: 2301 1651 1028 761 573 275 186 BsiHKAI G_wGCw'C Cuts at: 2377 2663 3159 4836 4921 5992 2377 Size: 286 496 1677 85 1071 3160 Fragments arranged by size: 3160 1677 1071 496 286 85 BsmI GAATG_Cn' Cuts at: 3387 4286 4379 3387 Size: 899 93 5783 Fragments arranged by size: 5783 899 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2377 2398 2479 2663 2671 2794 Size: 399 397 190 21 81 184 8 123 Cuts at: 2794 3159 3355 4012 4094 4836 4921 5992 1391 Size: 365 196 657 82 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 399 397 365 196 190 184 123 85 82 81 21 8 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2850 3712 4130 844 Size: 2006 862 418 3489 Fragments arranged by size: 3489 2006 862 418 BsrBI CCG'CTC Cuts at: 1394 1823 2368 2935 4662 1394 Size: 429 545 567 1727 3507 Fragments arranged by size: 3507 1727 567 545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3228 3235 5270 5444 65 Size: 1183 1980 7 2035 174 1396 Fragments arranged by size: 2035 1980 1396 1183 174 7 BsrFI r'CCGG_y Cuts at: 1700 1810 2266 2331 2423 5419 5606 1700 Size: 110 456 65 92 2996 187 2869 Fragments arranged by size: 2996 2869 456 187 110 92 65 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6775 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 2376 3698 4835 5847 2376 Size: 1322 1137 1012 3304 Fragments arranged by size: 3304 1322 1137 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2653 794 Size: 1859 4916 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 2495 3913 1184 Size: 1311 1418 4046 Fragments arranged by size: 4046 1418 1311 BstXI CCAn_nnnn'nTGG Cuts at: 2610 2610 Size: 6775 BstYI r'GATC_y Cuts at: 1972 2119 2292 2715 3039 3055 3285 4105 4472 Size: 147 173 423 324 16 230 820 367 Cuts at: 4472 4857 4874 6326 6412 6424 6770 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 820 423 385 367 346 324 230 173 147 86 17 16 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 3529 3618 3906 513 Size: 552 939 184 1341 89 288 3382 Fragments arranged by size: 3382 1341 939 552 288 184 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6036 Fragments arranged by size: 6036 683 56 BtsI GCAGTG_nn' Cuts at: 4291 5085 5105 4291 Size: 794 20 5961 Fragments arranged by size: 5961 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6775 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6740 DraI TTT'AAA Cuts at: 4079 4079 Size: 6775 DraIII CAC_nnn'GTG Cuts at: 2653 2830 3703 2653 Size: 177 873 5725 Fragments arranged by size: 5725 873 177 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5782 809 Size: 507 4466 1802 Fragments arranged by size: 4466 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2148 2286 Size: 54 1425 50 275 18 248 70 138 Cuts at: 2286 2586 2626 3615 3814 4222 5111 6740 8 Size: 300 40 989 199 408 889 1629 43 Fragments arranged by size: 1629 1425 989 889 408 300 275 248 199 138 70 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2882 4705 1353 Size: 1529 1823 3423 Fragments arranged by size: 3423 1823 1529 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3203 3440 5362 5736 5882 387 Size: 1064 138 1614 237 1922 374 146 1280 Fragments arranged by size: 1922 1614 1280 1064 374 237 146 138 EcoICRI GAG'CTC Cuts at: 2661 3157 2661 Size: 496 6279 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2501 2729 4087 4838 5435 6222 Size: 393 698 171 228 1358 751 597 787 Cuts at: 6222 6490 1239 Size: 268 1524 Fragments arranged by size: 1524 1358 787 751 698 597 393 268 228 171 EcoNI CCTnn'n_nnAGG Cuts at: 2778 2778 Size: 6775 EcoO109I rG'GnC_Cy Cuts at: 2059 2414 2476 2667 3351 3352 2059 Size: 355 62 191 684 1 5482 Fragments arranged by size: 5482 684 355 191 62 1 EcoRI G'AATT_C Cuts at: 4084 4462 4084 Size: 378 6397 FspI TGC'GCA Cuts at: 3566 5281 3566 Size: 1715 5060 HaeII r_GCGC'y Cuts at: 1678 2819 3271 3346 5922 1678 Size: 1141 452 75 2576 2531 Fragments arranged by size: 2576 2531 1141 452 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3427 3459 3674 3706 5416 Size: 32 135 32 1855 32 215 32 1710 Cuts at: 5416 5448 5490 5522 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1855 1710 215 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3331 3745 3982 4119 4365 669 Size: 988 281 1393 414 237 137 246 3079 Fragments arranged by size: 3079 1393 988 414 281 246 237 137 HindIII A'AGCT_T Cuts at: 748 748 Size: 6775 HpaI GTT'AAC Cuts at: 3745 4365 3745 Size: 620 6155 KpnI G_GTAC'C Cuts at: 4100 4100 Size: 6775 MfeI C'AATT_G Cuts at: 4374 4374 Size: 6775 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3528 5889 6073 1305 Size: 2223 2361 184 2007 Fragments arranged by size: 2361 2223 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2080 2150 2288 2588 3617 Size: 54 1425 343 248 70 138 300 1029 Cuts at: 3617 10 Size: 3168 Fragments arranged by size: 3168 1425 1029 343 300 248 138 70 54 MslI CAynn'nnrTG Cuts at: 518 1166 4733 5092 5251 518 Size: 648 3567 359 159 2042 Fragments arranged by size: 3567 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2207 2748 2981 3531 4191 4868 6016 6261 Size: 601 541 233 550 660 677 1148 245 Cuts at: 6261 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 660 601 550 541 245 233 NaeI GCC'GGC Cuts at: 2268 2333 2425 2268 Size: 65 92 6618 Fragments arranged by size: 6618 92 65 NcoI C'CATG_G Cuts at: 513 1065 2004 2188 3618 3906 513 Size: 552 939 184 1430 288 3382 Fragments arranged by size: 3382 1430 939 552 288 184 NdeI CA'TA_TG Cuts at: 387 387 Size: 6775 NgoMIV G'CCGG_C Cuts at: 2266 2331 2423 2266 Size: 65 92 6618 Fragments arranged by size: 6618 92 65 NheI G'CTAG_C Cuts at: 1052 3805 1052 Size: 2753 4022 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 2130 4133 5678 1132 Size: 97 901 2003 1545 2229 Fragments arranged by size: 2229 2003 1545 901 97 PasI CC'CwG_GG Cuts at: 1348 2178 3355 1348 Size: 830 1177 4768 Fragments arranged by size: 4768 1177 830 PciI A'CATG_T Cuts at: 2126 5674 2126 Size: 3548 3227 PflMI CCAn_nnn'nTGG Cuts at: 1108 3022 1108 Size: 1914 4861 PfoI T'CCnGG_A Cuts at: 2697 3897 2697 Size: 1200 5575 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PmlI CAC'GTG Cuts at: 3019 3019 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 4345 4345 Size: 6775 PspOMI G'GGCC_C Cuts at: 2475 2667 3351 4008 2475 Size: 192 684 657 5242 Fragments arranged by size: 5242 684 657 192 PstI C_TGCA'G Cuts at: 830 2733 2946 4127 830 Size: 1903 213 1181 3478 Fragments arranged by size: 3478 1903 1181 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 PvuII CAG'CTG Cuts at: 2207 2981 2207 Size: 774 6001 SacI G_AGCT'C Cuts at: 2663 3159 2663 Size: 496 6279 SacII CC_GC'GG Cuts at: 3532 3532 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 5023 6735 5023 Size: 1712 5063 SfcI C'TryA_G Cuts at: 826 1045 2729 2942 3462 4123 5258 5939 6130 Size: 219 1684 213 520 661 1135 681 191 Cuts at: 6130 826 Size: 1471 Fragments arranged by size: 1684 1471 1135 681 661 520 219 213 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 2456 2672 4102 1872 Size: 584 216 1430 4545 Fragments arranged by size: 4545 1430 584 216 SmlI C'TyrA_G Cuts at: 820 1017 1943 2326 4879 5780 6042 820 Size: 197 926 383 2553 901 262 1553 Fragments arranged by size: 2553 1553 926 901 383 262 197 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 4133 1132 Size: 3001 3774 SrfI GCCC'GGGC Cuts at: 2456 2456 Size: 6775 SspI AAT'ATT Cuts at: 5 52 4699 5 Size: 47 4647 2081 Fragments arranged by size: 4647 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 2004 2188 3495 3618 3906 4214 513 Size: 552 939 184 1307 123 288 308 3074 Fragments arranged by size: 3074 1307 939 552 308 288 184 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2170 2483 2841 3263 4802 4819 Size: 1255 417 173 313 358 422 1539 17 Cuts at: 4819 4972 5157 5602 325 Size: 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 445 422 417 358 313 185 173 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3994 5021 6733 Size: 274 80 33 51 1443 2017 1027 1712 Cuts at: 6733 96 Size: 138 Fragments arranged by size: 2017 1712 1443 1027 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 3195 3227 6735 6767 2211 Size: 32 952 32 3508 32 2219 Fragments arranged by size: 3508 2219 952 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6775 XbaI T'CTAG_A Cuts at: 4111 4111 Size: 6775 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2256 3073 3700 2256 Size: 817 627 5331 Fragments arranged by size: 5331 817 627 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6775 XmaI C'CCGG_G Cuts at: 1870 2454 2670 4100 1870 Size: 584 216 1430 4545 Fragments arranged by size: 4545 1430 584 216 XmnI GAAnn'nnTTC Cuts at: 4904 4904 Size: 6775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SwaI