(Circular) (Six-base) MAPSORT of: pFN21AE0450.seq Check: 4832 from: 1 to: 6049 pFN21AE0450 6049 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2440 2708 2440 Size: 268 5781 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2760 3859 278 Size: 53 83 186 936 1224 1099 2468 Fragments arranged by size: 2468 1224 1099 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3392 1937 Size: 1455 4594 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2719 2974 3158 4112 5496 5764 1632 Size: 953 134 255 184 954 1384 268 1917 Fragments arranged by size: 1917 1384 954 953 268 255 184 134 AflII C'TTAA_G Cuts at: 820 1017 2080 820 Size: 197 1063 4789 Fragments arranged by size: 4789 1063 197 AflIII A'CryG_T Cuts at: 1203 3131 4948 5925 1203 Size: 1928 1817 977 1327 Fragments arranged by size: 1928 1817 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 2342 1166 Size: 1176 4873 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AlwNI CAG_nnn'CTG Cuts at: 2620 2968 3792 5364 2620 Size: 348 824 1572 3305 Fragments arranged by size: 3305 1572 824 348 ApaI G_GGCC'C Cuts at: 2131 2131 Size: 6049 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 3358 3584 3736 5845 1493 Size: 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2555 3374 1870 Size: 73 612 819 4545 Fragments arranged by size: 4545 819 612 73 BamHI G'GATC_C Cuts at: 3379 3746 3379 Size: 367 5682 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 3091 3284 3370 4825 Size: 325 217 627 515 789 193 86 1455 Cuts at: 4825 618 Size: 1842 Fragments arranged by size: 1842 1455 789 627 515 325 217 193 86 BanII G_rGCy'C Cuts at: 1391 2131 2574 2585 3368 1391 Size: 740 443 11 783 4072 Fragments arranged by size: 4072 783 740 443 11 BbeI G_GCGC'C Cuts at: 2306 2306 Size: 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2650 2814 3034 928 Size: 971 751 164 220 3943 Fragments arranged by size: 3943 971 751 220 164 BbvCI CC'TCA_GC Cuts at: 2153 2436 2456 2153 Size: 283 20 5746 Fragments arranged by size: 5746 283 20 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4240 4274 1903 Size: 34 2303 34 3678 Fragments arranged by size: 3678 2303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 2934 1548 Size: 1386 4663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 2468 2610 Size: 107 122 71 631 605 706 90 142 Cuts at: 2610 3257 4660 136 Size: 647 1403 1525 Fragments arranged by size: 1525 1403 706 647 631 605 142 122 107 90 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 2846 3278 3466 2846 Size: 432 188 5429 Fragments arranged by size: 5429 432 188 Bme1580I G_kGCm'C Cuts at: 1790 2131 2874 3096 4110 5266 1790 Size: 341 743 222 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 743 341 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2196 3335 3780 Size: 416 53 83 399 24 779 1139 445 Cuts at: 3780 4738 442 Size: 958 1753 Fragments arranged by size: 1753 1139 958 779 445 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2254 4709 1239 Size: 1015 2455 2579 Fragments arranged by size: 2579 2455 1015 Bpu10I CC'TnA_GC Cuts at: 2153 2318 2436 2456 2153 Size: 165 118 20 5746 Fragments arranged by size: 5746 165 118 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2221 4174 5039 5337 2221 Size: 1953 865 298 2933 Fragments arranged by size: 2933 1953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2431 4712 882 Size: 617 932 2281 2219 Fragments arranged by size: 2281 2219 932 617 BsaAI yAC'GTr Cuts at: 493 1204 2869 3132 3840 493 Size: 711 1665 263 708 2702 Fragments arranged by size: 2702 1665 711 708 263 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2303 2370 2757 3856 Size: 53 83 186 936 770 67 387 1099 Cuts at: 3856 4238 4873 275 Size: 382 635 1451 Fragments arranged by size: 1451 1099 936 770 635 387 382 186 83 67 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 3236 3266 1178 Size: 30 165 30 816 30 987 30 3961 Fragments arranged by size: 3961 987 816 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2993 1186 Size: 1228 579 4242 Fragments arranged by size: 4242 1228 579 BseYI C'CCAG_C Cuts at: 2162 2586 2599 2961 3153 5252 5748 2162 Size: 424 13 362 192 2099 496 2463 Fragments arranged by size: 2463 2099 496 424 362 192 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3206 1163 Size: 573 1470 4006 Fragments arranged by size: 4006 1470 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3003 4110 4195 5266 3003 Size: 1107 85 1071 3786 Fragments arranged by size: 3786 1107 1071 85 BsmI GAATG_Cn' Cuts at: 3560 3653 3560 Size: 93 5956 BsmBI CGTCTCn'nnnn_ Cuts at: 2852 2852 Size: 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2574 2585 2874 3003 3096 3368 Size: 399 341 443 11 289 129 93 272 Cuts at: 3368 4110 4195 5266 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 341 289 272 129 93 85 11 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 2508 2708 2965 3404 844 Size: 1596 68 200 257 439 3489 Fragments arranged by size: 3489 1596 439 257 200 68 BsrBI CCG'CTC Cuts at: 1394 1823 2404 3282 3936 1394 Size: 429 581 878 654 3507 Fragments arranged by size: 3507 878 654 581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2904 4544 4718 65 Size: 1183 1656 1640 174 1396 Fragments arranged by size: 1656 1640 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 2363 4109 5121 2363 Size: 1746 1012 3291 Fragments arranged by size: 3291 1746 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6049 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 2323 1184 Size: 1139 4910 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2677 2887 2923 3076 2599 Size: 78 210 36 153 5572 Fragments arranged by size: 5572 210 153 78 36 BstYI r'GATC_y Cuts at: 1972 2883 3379 3746 4131 4148 5600 5686 5698 Size: 911 496 367 385 17 1452 86 12 Cuts at: 5698 6044 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 911 496 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2241 2241 Size: 6049 BtgI C'CryG_G Cuts at: 513 1065 2004 2120 2922 513 Size: 552 939 116 802 3640 Fragments arranged by size: 3640 939 802 552 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 2045 2157 3565 4359 4379 2045 Size: 112 1408 794 20 3715 Fragments arranged by size: 3715 1408 794 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2377 2412 529 Size: 35 1813 35 4166 Fragments arranged by size: 4166 1813 35 35 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2649 2766 2826 Size: 54 1425 50 275 18 819 117 60 Cuts at: 2826 3207 3313 3496 4385 6014 8 Size: 381 106 183 889 1629 43 Fragments arranged by size: 1629 1425 889 819 381 275 183 117 106 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 3023 3979 1353 Size: 1385 285 956 3423 Fragments arranged by size: 3423 1385 956 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3175 3218 4636 5010 5156 387 Size: 1064 138 1586 43 1418 374 146 1280 Fragments arranged by size: 1586 1418 1280 1064 374 146 138 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 2585 2719 2974 3158 4112 4709 Size: 393 622 331 134 255 184 954 597 Cuts at: 4709 5496 5764 1239 Size: 787 268 1524 Fragments arranged by size: 1524 954 787 622 597 393 331 268 255 184 134 EcoO109I rG'GnC_Cy Cuts at: 2075 2127 2380 2674 2892 3259 2075 Size: 52 253 294 218 367 4865 Fragments arranged by size: 4865 367 294 253 218 52 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2306 2463 3278 5196 1678 Size: 628 157 815 1918 2531 Fragments arranged by size: 2531 1918 815 628 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3236 3268 4690 4722 4764 Size: 32 135 32 1664 32 1422 32 42 Cuts at: 4764 4796 1373 Size: 32 2626 Fragments arranged by size: 2626 1664 1422 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2524 3393 3639 669 Size: 988 281 586 869 246 3079 Fragments arranged by size: 3079 988 869 586 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KasI G'GCGC_C Cuts at: 2302 2302 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5163 5347 1305 Size: 3858 184 2007 Fragments arranged by size: 3858 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2651 2768 3209 3315 10 Size: 54 1425 343 819 117 441 106 2744 Fragments arranged by size: 2744 1425 819 441 343 117 106 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 2342 2774 2921 4007 4366 4525 Size: 648 1095 81 432 147 1086 359 159 Cuts at: 4525 518 Size: 2042 Fragments arranged by size: 2042 1095 1086 648 432 359 159 147 81 MspA1I CmG'CkG Cuts at: 1606 2965 3157 3465 4142 5290 5535 1606 Size: 1359 192 308 677 1148 245 2120 Fragments arranged by size: 2120 1359 1148 677 308 245 192 NarI GG'CG_CC Cuts at: 2303 2303 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 2004 2120 513 Size: 552 939 116 4442 Fragments arranged by size: 4442 939 552 116 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2089 3407 4952 1132 Size: 97 860 1318 1545 2229 Fragments arranged by size: 2229 1545 1318 860 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2186 2620 1108 Size: 1078 434 4537 Fragments arranged by size: 4537 1078 434 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PmlI CAC'GTG Cuts at: 2869 3132 2869 Size: 263 5786 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2075 2892 2075 Size: 817 5232 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspOMI G'GGCC_C Cuts at: 2127 2127 Size: 6049 PspXI vC'TCGA_Gb Cuts at: 2555 2555 Size: 6049 PstI C_TGCA'G Cuts at: 830 2976 3219 3401 830 Size: 2146 243 182 3478 Fragments arranged by size: 3478 2146 243 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2965 2965 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SanDI GG'GwC_CC Cuts at: 2892 2892 Size: 6049 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SfcI C'TryA_G Cuts at: 826 1045 2833 2972 3215 3325 3397 4532 5213 Size: 219 1788 139 243 110 72 1135 681 Cuts at: 5213 5404 826 Size: 191 1471 Fragments arranged by size: 1788 1471 1135 681 243 219 191 139 110 72 SfiI GGCCn_nnn'nGGCC Cuts at: 2610 2610 Size: 6049 SfoI GGC'GCC Cuts at: 2304 2304 Size: 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 2200 2555 4153 5054 5316 Size: 197 926 137 120 355 1598 901 262 Cuts at: 5316 820 Size: 1553 Fragments arranged by size: 1598 1553 926 901 355 262 197 137 120 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 2089 3407 1132 Size: 957 1318 3774 Fragments arranged by size: 3774 1318 957 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 2004 2120 2131 2652 2670 3204 3303 Size: 552 939 116 11 521 18 534 99 Cuts at: 3303 3488 513 Size: 185 3074 Fragments arranged by size: 3074 939 552 534 521 185 116 99 18 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2529 4076 4093 4246 4431 4876 325 Size: 1255 949 1547 17 153 185 445 1498 Fragments arranged by size: 1547 1498 1255 949 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4295 6007 96 Size: 274 80 33 51 1443 2318 1712 138 Fragments arranged by size: 2318 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6009 6041 6009 Size: 32 6017 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3076 Size: 6049 XhoI C'TCGA_G Cuts at: 1943 2555 1943 Size: 612 5437 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2758 3857 276 Size: 53 83 186 936 1224 1099 2468 Fragments arranged by size: 2468 1224 1099 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacI SacII SapI SexAI SrfI SwaI