Restriction map for
pFN21AB8939
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfaNI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 340 AAATTC [GA]^AATT[CT]
AfaI 426 GTAC GT^AC
AlwI 204 GATCC GGATC(4/5)
ApaI 750 GGGCCC GGGCC^C
ApoI 340 AAATTC [GA]^AATT[CT]
BbeI 310 GGCGCC GGCGC^C
BbsI 645 GAAGAC GAAGAC(2/6)
BbvCI 696 CCTCAGC CCTCAGC(-5/-2)
BpmI 208 CTGGAG CTGGAG(16/14)
BpuAI 645 GAAGAC GAAGAC(2/6)
BsaI 376 GAGACC GGTCTC(1/5)
BsgI 727 CTGCAC GTGCAG(16/14)
BsmAI 376 GAGAC GTCTC(1/5)
BssSI 856 CTCGTG CACGAG(-5/-1)
BstYI 203 AGATCC [GA]^GATC[CT]
DdeI 697 CTCAG C^T[ACGT]AG
DpnII 204 GATC ^GATC
DraII 750 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 750 GGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 310 GGCGCC [GA]GCGC^[CT]
HgaI 660 GACGC GACGC(5/10)
KasI 310 GGCGCC G^GCGCC
KspI 177 CCGCGG CCGC^GG
MboI 204 GATC ^GATC
MboII 645 GAAGA GAAGA(8/7)
MflI 203 AGATCC [GA]^GATC[CT]
NarI 310 GGCGCC GG^CGCC
NcoI 813 CCATGG C^CATGG
NdeII 204 GATC ^GATC
PvuII 253 CAGCTG CAG^CTG
RsaI 426 GTAC GT^AC
SacI 193 GAGCTC GAGCT^C
SacII 177 CCGCGG CCGC^GG
Sau3AI 204 GATC ^GATC
SbfI 819 CCTGCAGG CCTGCA^GG
SfiI 159 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 310 GGCGCC GGC^GCC
Sse8387I 819 CCTGCAGG CCTGCA^GG
XcmI 155 CCAAGGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 203 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 310 GGCGCC G[GA]^CG[CT]C
660 GACGCC
AvaII 795 GGACC G^G[AT]CC
861 GGACC
BanI 310 GGCGCC G^G[CT][GA]CC
774 GGTGCC
BcnI 537 CCGGG CC^[GC]GG
748 CCGGG
BglI 160 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
708 GCCCCCTGGGC
BsaHI 310 GGCGCC G[GA]^CG[CT]C
660 GACGCC
BseRI 694 CTCCTC GAGGAG(10/8)
373 GAGGAG
BsrBI 82 GAGCGG CCGCTC(-3/-3)
791 GAGCGG
BsrFI 521 ACCGGC [GA]^CCGG[CT]
771 GCCGGT
BstXI 155 CCAAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
851 CCAACCTCGTGG
Cfr10I 521 ACCGGC [GA]^CCGG[CT]
771 GCCGGT
EaeI 297 CGGCCG [CT]^GGCC[GA]
542 CGGCCG
EagI 297 CGGCCG C^GGCCG
542 CGGCCG
EclXI 297 CGGCCG C^GGCCG
542 CGGCCG
Eco52I 297 CGGCCG C^GGCCG
542 CGGCCG
Hin1I 310 GGCGCC G[GA]^CG[CT]C
660 GACGCC
HphI 448 TCACC GGTGA(8/7)
488 TCACC
MslI 130 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
808 CACCACCATG
NciI 537 CCGGG CC^[GC]GG
748 CCGGG
SmlI 148 CTCAAG C^T[CT][GA]AG
262 CTCAAG
TaqI 80 TCGA T^CGA
278 TCGA
Tsp509I 341 AATT ^AATT
580 AATT
TthHB8I 80 TCGA T^CGA
278 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
BanII BbvI BmyI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NlaIII NlaIV PleI
PstI Sau96I ScrFI SfcI StyI
TseI TspRI