(Circular) (Six-base) MAPSORT of: pFN21AB8939.seq Check: 4058 from: 1 to: 5578 pFN21AB8939 5578 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3641 5025 5293 1632 Size: 2009 1384 268 1917 Fragments arranged by size: 2009 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 1184 Size: 1251 4327 AlwNI CAG_nnn'CTG Cuts at: 2260 2391 2514 3321 4893 2260 Size: 131 123 807 1572 2945 Fragments arranged by size: 2945 1572 807 131 123 ApaI G_GGCC'C Cuts at: 2759 2759 Size: 5578 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2345 2887 3113 3265 5374 1493 Size: 852 542 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 542 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2903 1870 Size: 73 960 4545 Fragments arranged by size: 4545 960 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 2779 2899 4354 618 Size: 325 217 627 528 464 120 1455 1842 Fragments arranged by size: 1842 1455 627 528 464 325 217 120 BanII G_rGCy'C Cuts at: 1391 2202 2759 2876 2897 1391 Size: 811 557 117 21 4072 Fragments arranged by size: 4072 811 557 117 21 BbeI G_GCGC'C Cuts at: 2319 2319 Size: 5578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2657 928 Size: 971 758 3849 Fragments arranged by size: 3849 971 758 BbvCI CC'TCA_GC Cuts at: 2702 2702 Size: 5578 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2171 2719 4189 Size: 107 122 71 631 605 499 548 1470 Cuts at: 4189 136 Size: 1525 Fragments arranged by size: 1525 1470 631 605 548 499 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 2759 3639 4795 1790 Size: 969 880 1156 2573 Fragments arranged by size: 2573 1156 969 880 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5578 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4267 442 Size: 416 53 83 399 24 1892 958 1753 Fragments arranged by size: 1892 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 4238 1239 Size: 995 2004 2579 Fragments arranged by size: 2579 2004 995 Bpu10I CC'TnA_GC Cuts at: 2702 2702 Size: 5578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 2252 3703 4568 4866 2138 Size: 114 1451 865 298 2850 Fragments arranged by size: 2850 1451 865 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 4241 882 Size: 617 876 1866 2219 Fragments arranged by size: 2219 1866 876 617 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2666 3385 3767 Size: 53 83 186 936 783 350 719 382 Cuts at: 3767 4402 275 Size: 635 1451 Fragments arranged by size: 1451 936 783 719 635 382 350 186 83 53 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2564 2594 2858 2888 1178 Size: 30 165 30 1161 30 264 30 3868 Fragments arranged by size: 3868 1161 264 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2690 1186 Size: 1207 297 4074 Fragments arranged by size: 4074 1207 297 BseYI C'CCAG_C Cuts at: 2092 2759 4781 5277 2092 Size: 667 2022 496 2393 Fragments arranged by size: 2393 2022 667 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2717 1163 Size: 573 981 4024 Fragments arranged by size: 4024 981 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 2542 2550 3028 3789 4817 Size: 275 186 304 237 8 478 761 1028 Cuts at: 4817 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 478 304 275 237 186 8 BsiHKAI G_wGCw'C Cuts at: 2130 2154 2202 3639 3724 4795 2130 Size: 24 48 1437 85 1071 2913 Fragments arranged by size: 2913 1437 1071 85 48 24 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2154 2202 2759 2876 2897 3639 Size: 399 340 24 48 557 117 21 742 Cuts at: 3639 3724 4795 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 399 340 117 85 48 24 21 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 844 Size: 2089 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2089 2798 3465 1394 Size: 429 266 709 667 3507 Fragments arranged by size: 3507 709 667 429 266 BsrDI GCAATG_nn' Cuts at: 65 1248 4073 4247 65 Size: 1183 2825 174 1396 Fragments arranged by size: 2825 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2526 2776 4222 4409 1700 Size: 110 716 250 1446 187 2869 Fragments arranged by size: 2869 1446 716 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 2861 3638 4650 2861 Size: 777 1012 3789 Fragments arranged by size: 3789 1012 777 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5578 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstXI CCAn_nnnn'nTGG Cuts at: 2167 2863 2167 Size: 696 4882 BstYI r'GATC_y Cuts at: 1972 2208 2908 3275 3660 3677 5129 5215 5227 Size: 236 700 367 385 17 1452 86 12 Cuts at: 5227 5573 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 700 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2166 2182 2319 2484 2818 513 Size: 552 1101 16 137 165 334 3273 Fragments arranged by size: 3273 1101 552 334 165 137 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2693 2772 2804 3094 3888 3908 2693 Size: 79 32 290 794 20 4363 Fragments arranged by size: 4363 794 290 79 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2547 3025 Size: 54 1425 50 275 18 472 245 478 Cuts at: 3025 3914 5543 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 478 472 275 245 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2302 2547 3025 1537 Size: 275 490 245 478 4090 Fragments arranged by size: 4090 490 478 275 245 EarI CTCTTCn'nnn_ Cuts at: 1353 3508 1353 Size: 2155 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2303 2492 2802 4165 4539 4685 Size: 1064 138 714 189 310 1363 374 146 Cuts at: 4685 387 Size: 1280 Fragments arranged by size: 1363 1280 1064 714 374 310 189 146 138 EcoICRI GAG'CTC Cuts at: 2200 2200 Size: 5578 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 3641 4238 5025 5293 1239 Size: 393 602 1407 597 787 268 1524 Fragments arranged by size: 1524 1407 787 602 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2756 2756 Size: 5578 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2319 4725 1678 Size: 641 2406 2531 Fragments arranged by size: 2531 2406 641 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 2858 2890 4219 Size: 32 135 32 1091 32 163 32 1329 Cuts at: 4219 4251 4293 4325 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 1091 163 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KasI G'GCGC_C Cuts at: 2315 2315 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4692 4876 1305 Size: 3387 184 2007 Fragments arranged by size: 3387 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2139 2817 3536 3895 4054 518 Size: 648 843 130 678 719 359 159 2042 Fragments arranged by size: 2042 843 719 678 648 359 159 130 MspA1I CmG'CkG Cuts at: 1606 2184 2260 2353 2605 2616 2994 3671 4819 Size: 578 76 93 252 11 378 677 1148 Cuts at: 4819 5064 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 578 378 252 245 93 76 11 NarI GG'CG_CC Cuts at: 2316 2316 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 2818 513 Size: 552 1753 3273 Fragments arranged by size: 3273 1753 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 2311 2717 1348 Size: 963 406 4209 Fragments arranged by size: 4209 963 406 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PfoI T'CCnGG_A Cuts at: 2211 2211 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PspOMI G'GGCC_C Cuts at: 2755 2755 Size: 5578 PstI C_TGCA'G Cuts at: 830 2070 2256 2829 2930 830 Size: 1240 186 573 101 3478 Fragments arranged by size: 3478 1240 573 186 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2260 2260 Size: 5578 SacI G_AGCT'C Cuts at: 2202 2202 Size: 5578 SacII CC_GC'GG Cuts at: 2185 2185 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SbfI CC_TGCA'GG Cuts at: 2829 2930 2829 Size: 101 5477 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SfcI C'TryA_G Cuts at: 826 1045 2066 2252 2825 2926 4061 4742 4933 Size: 219 1021 186 573 101 1135 681 191 Cuts at: 4933 826 Size: 1471 Fragments arranged by size: 1471 1135 1021 681 573 219 191 186 101 SfiI GGCCn_nnn'nGGCC Cuts at: 2171 2171 Size: 5578 SfoI GGC'GCC Cuts at: 2317 2317 Size: 5578 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 2267 3682 4583 4845 820 Size: 197 926 210 114 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 262 210 197 114 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2160 2556 2818 3017 513 Size: 552 1095 396 262 199 3074 Fragments arranged by size: 3074 1095 552 396 262 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3824 5536 96 Size: 274 80 33 51 1443 1847 1712 138 Fragments arranged by size: 1847 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2666 2698 5538 5570 2666 Size: 32 2840 32 2674 Fragments arranged by size: 2840 2674 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2167 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI