################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BbrPI BciVI BclI BfrI BglII BlnI BsiEI BsiWI Bsp1407I BspHI BspLU11I BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI MfeI MluI MseI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SapI SbfI SfiI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 73 GTATAC GT^[AC][GT]AC AccIII 803 TCCGGA T^CCGGA AcsI 1288 GAATTC [GA]^AATT[CT] ApoI 1288 GAATTC [GA]^AATT[CT] Asp700I 3267 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 618 GAGCAC G[AT]GC[AT]^C BcgI 3015 CGAACGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 2360 CTAG C^TAG BsaBI 2232 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 803 TCCGGA T^CCGGA BsiHKAI 618 GAGCAC G[AT]GC[AT]^C BsmBI 3147 CGTCTC CGTCTC(1/5) BspEI 803 TCCGGA T^CCGGA Bst1107I 73 GTATAC GTA^TAC BstZ17I 73 GTATAC GTA^TAC CpoI 2180 CGGTCCG CG^G[AT]CCG DraIII 335 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1102 GACAGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1288 GAATTC G^AATTC FseI 918 GGCCGGCC GGCCGG^CC MaeI 2360 CTAG C^TAG MamI 2232 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 803 TCCGGA T^CCGGA NcoI 3206 CCATGG C^CATGG NdeI 1796 CATATG CA^TATG NspI 2455 ACATGC [GA]CATG^[CT] RsrII 2180 CGGTCCG CG^G[AT]CCG ScaI 1271 AGTACT AGT^ACT SexAI 91 ACCTGGT A^CC[AT]GGT SnaBI 70 TACGTA TAC^GTA Tsp509I 1289 AATT ^AATT XmnI 3267 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AhdI 1807 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2154 GACCTCCTGTC AocI 1655 CCTCAGG CC^T[ACGT]AGG 2795 CCTCAGG AspEI 1807 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2154 GACCTCCTGTC AspI 90 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 343 GACGAAGTC BalI 186 TGGCCA TGG^CCA 1142 TGGCCA BbeI 858 GGCGCC GGCGC^C 2252 GGCGCC BbsI 349 GTCTTC GAAGAC(2/6) 1737 GTCTTC BbvCI 1156 CCTCAGC CCTCAGC(-5/-2) 1973 CCTCAGC BlpI 552 GCTGAGC GC^T[ACGT]AGC 1626 GCTGAGC Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC 1626 GCTGAGC BpuAI 349 GTCTTC GAAGAC(2/6) 1737 GTCTTC BsaI 1765 GAGACC GGTCTC(1/5) 2262 GAGACC BsmI 1941 GAATGC GAATGC(1/-1) 1600 GCATTC BspDI 289 ATCGAT AT^CGAT 1420 ATCGAT BstYI 233 AGATCC [GA]^GATC[CT] 3251 AGATCC Bsu36I 1655 CCTCAGG CC^T[ACGT]AGG 2795 CCTCAGG CelII 552 GCTGAGC GC^T[ACGT]AGC 1626 GCTGAGC ClaI 289 ATCGAT AT^CGAT 1420 ATCGAT Eam1105I 1807 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2154 GACCTCCTGTC Eco81I 1655 CCTCAGG CC^T[ACGT]AGG 2795 CCTCAGG EcoNI 873 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1755 CCTGGAAGAGG HincII 382 GTCAAC GT[CT]^[GA]AC 2731 GTCAAC HindII 382 GTCAAC GT[CT]^[GA]AC 2731 GTCAAC KasI 858 GGCGCC G^GCGCC 2252 GGCGCC MflI 233 AGATCC [GA]^GATC[CT] 3251 AGATCC MluNI 186 TGGCCA TGG^CCA 1142 TGGCCA MscI 186 TGGCCA TGG^CCA 1142 TGGCCA MslI 27 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3072 CATACCCGTG NaeI 919 GCCGGC GCC^GGC 1695 GCCGGC NarI 858 GGCGCC GG^CGCC 2252 GGCGCC NgoMIV 919 GCCGGC G^CCGGC 1695 GCCGGC PflFI 90 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 343 GACGAAGTC PflMI 2589 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3249 CCAGATCCTGG PpuMI 194 AGGACCC [GA]G^G[AT]CC[CT] 641 GGGTCCC PstI 148 CTGCAG CTGCA^G 1708 CTGCAG SfcI 148 CTGCAG C^T[GA][CT]AG 1708 CTGCAG SfoI 858 GGCGCC GGC^GCC 2252 GGCGCC SmaI 2165 CCCGGG CCC^GGG 2776 CCCGGG Tth111I 90 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 343 GACGAAGTC Van91I 2589 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3249 CCAGATCCTGG XhoII 233 AGATCC [GA]^GATC[CT] 3251 AGATCC XmaCI 2165 CCCGGG C^CCGGG 2776 CCCGGG XmaI 2165 CCCGGG C^CCGGG 2776 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI