(Circular) (Six-base) MAPSORT of: pFN21AE2667.seq Check: 9706 from: 1 to: 8020 pFN21AE2667 8020 bp With 182 enzymes: SgfI * January 20, 2017 10:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4513 4831 4513 Size: 318 7702 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5830 278 Size: 53 83 186 936 4294 2468 Fragments arranged by size: 4294 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 5363 1937 Size: 142 3284 4594 Fragments arranged by size: 4594 3284 142 Acc65I G'GTAC_C Cuts at: 5341 5341 Size: 8020 AclI AA'CG_TT Cuts at: 1563 5319 6147 6520 1563 Size: 3756 828 373 3063 Fragments arranged by size: 3756 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 2900 3729 4094 6083 7467 7735 1632 Size: 674 594 829 365 1989 1384 268 1917 Fragments arranged by size: 1989 1917 1384 829 674 594 365 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7823 AflIII A'CryG_T Cuts at: 1203 6919 7896 1203 Size: 5716 977 1327 Fragments arranged by size: 5716 1327 977 AgeI A'CCGG_T Cuts at: 6851 6851 Size: 8020 AhdI GACnn_n'nnGTC Cuts at: 3817 4164 6749 3817 Size: 347 2585 5088 Fragments arranged by size: 5088 2585 347 AleI CACnn'nnGTG Cuts at: 1166 2036 1166 Size: 870 7150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8020 AlwNI CAG_nnn'CTG Cuts at: 3626 4528 5260 5763 7335 3626 Size: 902 732 503 1572 4311 Fragments arranged by size: 4311 1572 902 732 503 ApaI G_GGCC'C Cuts at: 3172 3412 3533 3724 4588 3172 Size: 240 121 191 864 6604 Fragments arranged by size: 6604 864 240 191 121 ApaLI G'TGCA_C Cuts at: 6077 7233 6077 Size: 1156 6864 ApoI r'AATT_y Cuts at: 1493 3293 5329 5555 5707 7816 1493 Size: 1800 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1800 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6574 160 Size: 872 5542 1606 Fragments arranged by size: 5542 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8020 AvaI C'yCGr_G Cuts at: 1870 1943 2378 2682 4170 4781 5345 1870 Size: 73 435 304 1488 611 564 4545 Fragments arranged by size: 4545 1488 611 564 435 304 73 BamHI G'GATC_C Cuts at: 5350 5717 5350 Size: 367 7653 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2477 2863 3484 4257 5290 Size: 325 217 627 690 386 621 773 1033 Cuts at: 5290 5341 6796 618 Size: 51 1455 1842 Fragments arranged by size: 1842 1455 1033 773 690 627 621 386 325 217 51 BanII G_rGCy'C Cuts at: 1391 2499 3137 3172 3258 3412 3533 3645 3724 Size: 1108 638 35 86 154 121 112 79 Cuts at: 3724 4296 4304 4588 5339 1391 Size: 572 8 284 751 4072 Fragments arranged by size: 4072 1108 751 638 572 284 154 121 112 86 79 35 8 BbeI G_GCGC'C Cuts at: 2867 4261 2867 Size: 1394 6626 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2347 3735 928 Size: 971 448 1388 5213 Fragments arranged by size: 5213 1388 971 448 BbvCI CC'TCA_GC Cuts at: 3162 3979 3162 Size: 817 7203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5009 5043 6211 6245 1903 Size: 34 50 34 2988 34 1168 34 3678 Fragments arranged by size: 3678 2988 1168 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5912 7122 5912 Size: 1210 6810 BclI T'GATC_A Cuts at: 1548 1548 Size: 8020 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3691 4346 4950 Size: 107 122 71 631 605 2019 655 604 Cuts at: 4950 5000 5024 6631 136 Size: 50 24 1607 1525 Fragments arranged by size: 2019 1607 1525 655 631 605 604 122 107 71 50 24 BglII A'GATC_T Cuts at: 8015 8015 Size: 8020 BlpI GC'TnA_GC Cuts at: 2558 3632 5437 2558 Size: 1074 1805 5141 Fragments arranged by size: 5141 1805 1074 Bme1580I G_kGCm'C Cuts at: 1790 2314 3172 3412 3489 3533 3724 4588 4659 Size: 524 858 240 77 44 191 864 71 Cuts at: 4659 6081 7237 1790 Size: 1422 1156 2573 Fragments arranged by size: 2573 1422 1156 864 858 524 240 191 77 71 44 BmgBI CAC'GTC Cuts at: 1318 2044 2470 1318 Size: 726 426 6868 Fragments arranged by size: 6868 726 426 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2924 4347 4852 Size: 416 53 83 399 24 1507 1423 505 Cuts at: 4852 5751 6709 442 Size: 899 958 1753 Fragments arranged by size: 1753 1507 1423 958 899 505 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8020 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2549 2684 2736 3103 3123 4550 4595 4718 Size: 1310 135 52 367 20 1427 45 123 Cuts at: 4718 4794 5243 6680 1239 Size: 76 449 1437 2579 Fragments arranged by size: 2579 1437 1427 1310 449 367 135 123 76 52 45 20 Bpu10I CC'TnA_GC Cuts at: 3162 3449 3627 3979 3162 Size: 287 178 352 7203 Fragments arranged by size: 7203 352 287 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3557 3917 4280 5327 6145 7010 7308 3557 Size: 360 363 1047 818 865 298 4269 Fragments arranged by size: 4269 1047 865 818 363 360 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3764 4261 6683 882 Size: 617 2265 497 2422 2219 Fragments arranged by size: 2422 2265 2219 617 497 BsaAI yAC'GTr Cuts at: 493 1204 2077 2518 3382 5811 493 Size: 711 873 441 864 2429 2702 Fragments arranged by size: 2702 2429 873 864 711 441 BsaBI GATnn'nnATC Cuts at: 4241 8014 4241 Size: 3773 4247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2484 2790 2864 2984 Size: 53 83 186 936 951 306 74 120 Cuts at: 2984 4258 4953 5827 6209 6844 275 Size: 1274 695 874 382 635 1451 Fragments arranged by size: 1451 1274 951 936 874 695 635 382 306 186 120 83 74 53 BsaWI w'CCGG_w Cuts at: 2127 2358 2808 6453 6851 7125 7272 7683 2127 Size: 231 450 3645 398 274 147 411 2464 Fragments arranged by size: 3645 2464 450 411 398 274 231 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 3187 3217 3575 Size: 30 165 30 693 30 1061 30 358 Cuts at: 3575 3605 3833 3863 4532 4562 5192 5222 1178 Size: 30 228 30 669 30 630 30 3976 Fragments arranged by size: 3976 1061 693 669 630 358 228 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 2738 3539 3596 3653 3782 3823 3853 Size: 1462 90 801 57 57 129 41 30 Cuts at: 3853 3967 4015 4018 4027 4030 4033 4957 5195 Size: 114 48 3 9 3 3 924 238 Cuts at: 5195 1186 Size: 4011 Fragments arranged by size: 4011 1462 924 801 238 129 114 90 57 57 48 41 30 9 3 3 3 BseYI C'CCAG_C Cuts at: 2445 2643 2859 3405 3503 3681 4151 4396 4581 Size: 198 216 546 98 178 470 245 185 Cuts at: 4581 5064 5286 7223 7719 2445 Size: 483 222 1937 496 2746 Fragments arranged by size: 2746 1937 546 496 483 470 245 222 216 198 185 178 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2762 3404 4541 1163 Size: 573 306 720 642 1137 4642 Fragments arranged by size: 4642 1137 720 642 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5470 6231 7259 1540 Size: 275 186 3469 761 1028 2301 Fragments arranged by size: 3469 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2627 6081 6166 7237 2627 Size: 3454 85 1071 3410 Fragments arranged by size: 3454 3410 1071 85 BsmI GAATG_Cn' Cuts at: 3605 3952 5531 5624 3605 Size: 347 1579 93 6001 Fragments arranged by size: 6001 1579 347 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5158 5158 Size: 8020 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2314 2499 2627 3137 3172 3258 3412 Size: 399 524 185 128 510 35 86 154 Cuts at: 3412 3489 3533 3645 3724 4296 4304 4588 4659 Size: 77 44 112 79 572 8 284 71 Cuts at: 4659 5339 6081 6166 7237 1391 Size: 680 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 680 572 524 510 399 284 185 154 128 112 86 85 79 77 71 44 35 8 BspEI T'CCGG_A Cuts at: 2808 7683 2808 Size: 4875 3145 BspHI T'CATG_A Cuts at: 5909 7639 5909 Size: 1730 6290 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2907 2976 4513 4831 5375 844 Size: 1316 747 69 1537 318 544 3489 Fragments arranged by size: 3489 1537 1316 747 544 318 69 BsrBI CCG'CTC Cuts at: 1394 1823 2607 4083 4545 5907 1394 Size: 429 784 1476 462 1362 3507 Fragments arranged by size: 3507 1476 1362 784 462 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3036 3079 3285 6515 6689 65 Size: 1183 1788 43 206 3230 174 1396 Fragments arranged by size: 3230 1788 1396 1183 206 174 43 BsrFI r'CCGG_y Cuts at: 1700 1810 2924 3690 3700 6664 6851 1700 Size: 110 1114 766 10 2964 187 2869 Fragments arranged by size: 2964 2869 1114 766 187 110 10 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8020 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8020 BssSI C'ACGA_G Cuts at: 6080 7092 6080 Size: 1012 7008 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8020 BstBI TT'CG_AA Cuts at: 6824 6824 Size: 8020 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8020 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2506 2662 4286 2038 Size: 468 156 1624 5772 Fragments arranged by size: 5772 1624 468 156 BstYI r'GATC_y Cuts at: 1972 2238 5256 5350 5717 6102 6119 7571 7657 Size: 266 3018 94 367 385 17 1452 86 Cuts at: 7657 7669 8015 1972 Size: 12 346 1977 Fragments arranged by size: 3018 1977 1452 385 367 346 266 94 86 17 12 BstZ17I GTA'TAC Cuts at: 2080 2080 Size: 8020 Bsu36I CC'TnA_GG Cuts at: 3661 4801 3661 Size: 1140 6880 BtgI C'CryG_G Cuts at: 513 1065 2034 3093 3563 3645 4105 4285 4506 Size: 552 969 1059 470 82 460 180 221 Cuts at: 4506 5211 513 Size: 705 3322 Fragments arranged by size: 3322 1059 969 705 552 470 460 221 180 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7281 Fragments arranged by size: 7281 683 56 BtsI GCAGTG_nn' Cuts at: 3094 4977 5536 6330 6350 3094 Size: 1883 559 794 20 4764 Fragments arranged by size: 4764 1883 794 559 20 ClaI AT'CG_AT Cuts at: 1554 2295 3426 1554 Size: 741 1131 6148 Fragments arranged by size: 6148 1131 741 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4045 4080 529 Size: 35 3481 35 4469 Fragments arranged by size: 4469 3481 35 35 DraI TTT'AAA Cuts at: 5324 5324 Size: 8020 DraIII CAC_nnn'GTG Cuts at: 2345 2345 Size: 8020 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3113 7027 809 Size: 507 1797 3914 1802 Fragments arranged by size: 3914 1802 1797 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 2922 3147 Size: 54 1425 50 275 18 361 731 225 Cuts at: 3147 3797 4932 5016 5467 6356 7985 8 Size: 650 1135 84 451 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 731 650 451 361 275 225 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5467 1537 Size: 275 3655 4090 Fragments arranged by size: 4090 3655 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2273 2715 3712 3759 4036 4051 5950 Size: 896 24 442 997 47 277 15 1899 Cuts at: 5950 1353 Size: 3423 Fragments arranged by size: 3423 1899 997 896 442 277 47 24 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6607 6981 7127 387 Size: 1064 138 5018 374 146 1280 Fragments arranged by size: 5018 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2549 2684 2736 2900 3103 3123 Size: 393 674 243 135 52 164 203 20 Cuts at: 3123 3729 4094 4550 4595 4718 4794 5243 6083 Size: 606 365 456 45 123 76 449 840 Cuts at: 6083 6680 7467 7735 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 840 787 674 606 597 456 449 393 365 268 243 203 164 135 123 76 52 45 20 EcoNI CCTnn'n_nnAGG Cuts at: 2882 3764 2882 Size: 882 7138 EcoO109I rG'GnC_Cy Cuts at: 2200 2647 3031 3409 3529 3530 4585 4854 2200 Size: 447 384 378 120 1 1055 269 5366 Fragments arranged by size: 5366 1055 447 384 378 269 120 1 EcoRI G'AATT_C Cuts at: 3293 5329 5707 3293 Size: 2036 378 5606 Fragments arranged by size: 5606 2036 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 8020 FseI GG_CCGG'CC Cuts at: 2928 2928 Size: 8020 FspI TGC'GCA Cuts at: 6526 6526 Size: 8020 HaeII r_GCGC'y Cuts at: 1678 2867 4261 4470 7167 1678 Size: 1189 1394 209 2697 2531 Fragments arranged by size: 2697 2531 1394 1189 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 3145 3177 3187 Size: 32 135 32 524 32 1017 32 10 Cuts at: 3187 3219 3347 3379 3644 3676 3803 3833 3835 Size: 32 128 32 265 32 127 30 2 Cuts at: 3835 3865 4150 4182 5192 5224 6661 6693 6735 Size: 30 285 32 1010 32 1437 32 42 Cuts at: 6735 6767 1373 Size: 32 2626 Fragments arranged by size: 2626 1437 1017 1010 524 285 265 135 128 127 42 32 32 32 32 32 32 32 32 32 32 32 30 30 10 2 HincII GTy'rAC Cuts at: 669 1657 1938 2389 4738 5364 5610 669 Size: 988 281 451 2349 626 246 3079 Fragments arranged by size: 3079 2349 988 626 451 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8020 HpaI GTT'AAC Cuts at: 5610 5610 Size: 8020 KasI G'GCGC_C Cuts at: 2863 4257 2863 Size: 1394 6626 KpnI G_GTAC'C Cuts at: 5345 5345 Size: 8020 MfeI C'AATT_G Cuts at: 5619 5619 Size: 8020 MluI A'CGCG_T Cuts at: 7896 7896 Size: 8020 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 2718 3259 4404 4981 5322 7134 7318 Size: 1248 165 541 1145 577 341 1812 184 Cuts at: 7318 1305 Size: 2007 Fragments arranged by size: 2007 1812 1248 1145 577 541 341 184 165 MscI TGG'CCA Cuts at: 10 64 1489 1832 2193 3149 10 Size: 54 1425 343 361 956 4881 Fragments arranged by size: 4881 1425 956 361 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 5081 5978 6337 6496 518 Size: 648 870 3045 897 359 159 2042 Fragments arranged by size: 3045 2042 897 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2445 3143 3166 3565 3718 4107 4264 4508 Size: 839 698 23 399 153 389 157 244 Cuts at: 4508 4814 4991 5436 6113 7261 7506 1606 Size: 306 177 445 677 1148 245 2120 Fragments arranged by size: 2120 1148 839 698 677 445 399 389 306 245 244 177 157 153 23 NaeI GCC'GGC Cuts at: 2926 3702 2926 Size: 776 7244 NarI GG'CG_CC Cuts at: 2864 4258 2864 Size: 1394 6626 NcoI C'CATG_G Cuts at: 513 1065 5211 513 Size: 552 4146 3322 Fragments arranged by size: 4146 3322 552 NdeI CA'TA_TG Cuts at: 387 3802 387 Size: 3415 4605 NgoMIV G'CCGG_C Cuts at: 2924 3700 2924 Size: 776 7244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8020 NotI GC'GGCC_GC Cuts at: 5467 5467 Size: 8020 NspI r_CATG'y Cuts at: 1132 1229 4464 5378 6923 1132 Size: 97 3235 914 1545 2229 Fragments arranged by size: 3235 2229 1545 914 97 PasI CC'CwG_GG Cuts at: 1348 3130 1348 Size: 1782 6238 PciI A'CATG_T Cuts at: 6919 6919 Size: 8020 PflMI CCAn_nnn'nTGG Cuts at: 1108 4600 5260 1108 Size: 3492 660 3868 Fragments arranged by size: 3868 3492 660 PfoI T'CCnGG_A Cuts at: 2262 2505 4482 5196 5259 2262 Size: 243 1977 714 63 5023 Fragments arranged by size: 5023 1977 714 243 63 PmeI GTTT'AAAC Cuts at: 5324 5324 Size: 8020 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 6089 6121 7602 7634 3962 Size: 32 2095 32 1481 32 4348 Fragments arranged by size: 4348 2095 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2647 2200 Size: 447 7573 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8020 PsiI TTA'TAA Cuts at: 5590 5590 Size: 8020 PspOMI G'GGCC_C Cuts at: 3168 3408 3529 3720 4584 3168 Size: 240 121 191 864 6604 Fragments arranged by size: 6604 864 240 191 121 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2126 2158 2126 Size: 32 7988 PstI C_TGCA'G Cuts at: 830 2157 3717 5372 830 Size: 1327 1560 1655 3478 Fragments arranged by size: 3478 1655 1560 1327 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8020 PvuII CAG'CTG Cuts at: 2445 3143 4264 2445 Size: 698 1121 6201 Fragments arranged by size: 6201 1121 698 RsrII CG'GwC_CG Cuts at: 4186 4186 Size: 8020 SacII CC_GC'GG Cuts at: 3566 4108 4509 3566 Size: 542 401 7077 Fragments arranged by size: 7077 542 401 SalI G'TCGA_C Cuts at: 1936 5362 1936 Size: 3426 4594 SanDI GG'GwC_CC Cuts at: 2647 2647 Size: 8020 SbfI CC_TGCA'GG Cuts at: 5372 5372 Size: 8020 ScaI AGT'ACT Cuts at: 3278 6268 7980 3278 Size: 2990 1712 3318 Fragments arranged by size: 3318 2990 1712 SexAI A'CCwGG_T Cuts at: 2096 2096 Size: 8020 SfcI C'TryA_G Cuts at: 826 1045 2153 3713 5368 6503 7184 7375 826 Size: 219 1108 1560 1655 1135 681 191 1471 Fragments arranged by size: 1655 1560 1471 1135 1108 681 219 191 SfoI GGC'GCC Cuts at: 2865 4259 2865 Size: 1394 6626 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8020 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8020 SmaI CCC'GGG Cuts at: 1872 4172 4783 5347 1872 Size: 2300 611 564 4545 Fragments arranged by size: 4545 2300 611 564 SmlI C'TyrA_G Cuts at: 820 1017 1943 3572 3932 4295 5306 6124 7025 Size: 197 926 1629 360 363 1011 818 901 Cuts at: 7025 7287 820 Size: 262 1553 Fragments arranged by size: 1629 1553 1011 926 901 818 363 360 262 197 SnaBI TAC'GTA Cuts at: 493 2077 5811 493 Size: 1584 3734 2702 Fragments arranged by size: 3734 2702 1584 SpeI A'CTAG_T Cuts at: 152 152 Size: 8020 SphI G_CATG'C Cuts at: 1132 5378 1132 Size: 4246 3774 SspI AAT'ATT Cuts at: 5 52 5944 5 Size: 47 5892 2081 Fragments arranged by size: 5892 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8020 StyI C'CwwG_G Cuts at: 513 1065 2889 3480 3513 4116 4662 4884 5004 Size: 552 1824 591 33 603 546 222 120 Cuts at: 5004 5211 5459 513 Size: 207 248 3074 Fragments arranged by size: 3074 1824 603 591 552 546 248 222 207 120 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 4174 4897 5278 6047 6064 6217 Size: 1255 2145 449 723 381 769 17 153 Cuts at: 6217 6402 6847 325 Size: 185 445 1498 Fragments arranged by size: 2145 1498 1255 769 723 449 445 381 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3276 6266 7978 Size: 274 80 33 51 1443 1299 2990 1712 Cuts at: 7978 96 Size: 138 Fragments arranged by size: 2990 1712 1443 1299 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2813 2845 4532 4564 7980 8012 2813 Size: 32 1687 32 3416 32 2821 Fragments arranged by size: 3416 2821 1687 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2098 2351 1316 Size: 782 253 6985 Fragments arranged by size: 6985 782 253 XbaI T'CTAG_A Cuts at: 5356 5356 Size: 8020 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5011 5218 5261 5011 Size: 207 43 7770 Fragments arranged by size: 7770 207 43 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8020 XmaI C'CCGG_G Cuts at: 1870 4170 4781 5345 1870 Size: 2300 611 564 4545 Fragments arranged by size: 4545 2300 611 564 XmnI GAAnn'nnTTC Cuts at: 5276 6149 5276 Size: 873 7147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5828 276 Size: 53 83 186 936 4294 2468 Fragments arranged by size: 4294 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI EcoICRI EcoRV FspAI NruI NsiI PacI PmlI PspXI SacI SapI SfiI SrfI SwaI