(Linear) (Six-base) MAPSORT of: KIEE2667.seq Check: 2438 from: 1 to: 3315 KIEE2667.orf With 182 enzymes: SgfI * January 20, 2017 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2508 2826 3315 Size: 2508 318 489 Fragments arranged by size: 2508 489 318 AccI GT'mk_AC Cuts at: 0 74 3315 Size: 74 3241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 895 1724 2089 3315 Size: 301 594 829 365 1226 Fragments arranged by size: 1226 829 594 365 301 AhdI GACnn_n'nnGTC Cuts at: 0 1812 2159 3315 Size: 1812 347 1156 Fragments arranged by size: 1812 1156 347 AleI CACnn'nnGTG Cuts at: 0 31 3315 Size: 31 3284 AlwNI CAG_nnn'CTG Cuts at: 0 1621 2523 3255 3315 Size: 1621 902 732 60 Fragments arranged by size: 1621 902 732 60 ApaI G_GGCC'C Cuts at: 0 1167 1407 1528 1719 2583 3315 Size: 1167 240 121 191 864 732 Fragments arranged by size: 1167 864 732 240 191 121 ApoI r'AATT_y Cuts at: 0 1288 3315 Size: 1288 2027 AvaI C'yCGr_G Cuts at: 0 373 677 2165 2776 3315 Size: 373 304 1488 611 539 Fragments arranged by size: 1488 611 539 373 304 BanI G'GyrC_C Cuts at: 0 472 858 1479 2252 3285 3315 Size: 472 386 621 773 1033 30 Fragments arranged by size: 1033 773 621 472 386 30 BanII G_rGCy'C Cuts at: 0 494 1132 1167 1253 1407 1528 1640 1719 Size: 494 638 35 86 154 121 112 79 Cuts at: 1719 2291 2299 2583 3315 Size: 572 8 284 732 Fragments arranged by size: 732 638 572 494 284 154 121 112 86 79 35 8 BbeI G_GCGC'C Cuts at: 0 862 2256 3315 Size: 862 1394 1059 Fragments arranged by size: 1394 1059 862 BbsI GAAGACnn'nnnn_ Cuts at: 0 342 1730 3315 Size: 342 1388 1585 Fragments arranged by size: 1585 1388 342 BbvCI CC'TCA_GC Cuts at: 0 1157 1974 3315 Size: 1157 817 1341 Fragments arranged by size: 1341 1157 817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3004 3038 3315 Size: 3004 34 277 Fragments arranged by size: 3004 277 34 BglI GCCn_nnn'nGGC Cuts at: 0 1686 2341 2945 2995 3019 3315 Size: 1686 655 604 50 24 296 Fragments arranged by size: 1686 655 604 296 50 24 BlpI GC'TnA_GC Cuts at: 0 553 1627 3315 Size: 553 1074 1688 Fragments arranged by size: 1688 1074 553 Bme1580I G_kGCm'C Cuts at: 0 309 1167 1407 1484 1528 1719 2583 2654 Size: 309 858 240 77 44 191 864 71 Cuts at: 2654 3315 Size: 661 Fragments arranged by size: 864 858 661 309 240 191 77 71 44 BmgBI CAC'GTC Cuts at: 0 39 465 3315 Size: 39 426 2850 Fragments arranged by size: 2850 426 39 BmrI ACTGGGnnnn_n' Cuts at: 0 919 2342 2847 3315 Size: 919 1423 505 468 Fragments arranged by size: 1423 919 505 468 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 544 679 731 1098 1118 2545 2590 2713 Size: 544 135 52 367 20 1427 45 123 Cuts at: 2713 2789 3238 3315 Size: 76 449 77 Fragments arranged by size: 1427 544 449 367 135 123 77 76 52 45 20 Bpu10I CC'TnA_GC Cuts at: 0 1157 1444 1622 1974 3315 Size: 1157 287 178 352 1341 Fragments arranged by size: 1341 1157 352 287 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1552 1912 2275 3315 Size: 1552 360 363 1040 Fragments arranged by size: 1552 1040 363 360 BsaI GGTCTCn'nnnn_ Cuts at: 0 1759 2256 3315 Size: 1759 497 1059 Fragments arranged by size: 1759 1059 497 BsaAI yAC'GTr Cuts at: 0 72 513 1377 3315 Size: 72 441 864 1938 Fragments arranged by size: 1938 864 441 72 BsaBI GATnn'nnATC Cuts at: 0 2236 3315 Size: 2236 1079 BsaHI Gr'CG_yC Cuts at: 0 479 785 859 979 2253 2948 3315 Size: 479 306 74 120 1274 695 367 Fragments arranged by size: 1274 695 479 367 306 120 74 BsaWI w'CCGG_w Cuts at: 0 122 353 803 3315 Size: 122 231 450 2512 Fragments arranged by size: 2512 450 231 122 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 91 121 1182 1212 1570 1600 1828 1858 Size: 91 30 1061 30 358 30 228 30 Cuts at: 1858 2527 2557 3187 3217 3315 Size: 669 30 630 30 98 Fragments arranged by size: 1061 669 630 358 228 98 91 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 643 733 1534 1591 1648 1777 1818 1848 Size: 643 90 801 57 57 129 41 30 Cuts at: 1848 1962 2010 2013 2022 2025 2028 2952 3190 Size: 114 48 3 9 3 3 924 238 Cuts at: 3190 3315 Size: 125 Fragments arranged by size: 924 801 643 238 129 125 114 90 57 57 48 41 30 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 440 638 854 1400 1498 1676 2146 2391 Size: 440 198 216 546 98 178 470 245 Cuts at: 2391 2576 3059 3281 3315 Size: 185 483 222 34 Fragments arranged by size: 546 483 470 440 245 222 216 198 185 178 98 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 757 1399 2536 3315 Size: 37 720 642 1137 779 Fragments arranged by size: 1137 779 720 642 37 BsiHKAI G_wGCw'C Cuts at: 0 622 3315 Size: 622 2693 BsmI GAATG_Cn' Cuts at: 0 1600 1947 3315 Size: 1600 347 1368 Fragments arranged by size: 1600 1368 347 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3153 3315 Size: 3153 162 Bsp1286I G_dGCh'C Cuts at: 0 309 494 622 1132 1167 1253 1407 1484 Size: 309 185 128 510 35 86 154 77 Cuts at: 1484 1528 1640 1719 2291 2299 2583 2654 3315 Size: 44 112 79 572 8 284 71 661 Fragments arranged by size: 661 572 510 309 284 185 154 128 112 86 79 77 71 44 35 8 BspEI T'CCGG_A Cuts at: 0 803 3315 Size: 803 2512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 155 902 971 2508 2826 3315 Size: 155 747 69 1537 318 489 Fragments arranged by size: 1537 747 489 318 155 69 BsrBI CCG'CTC Cuts at: 0 602 2078 2540 3315 Size: 602 1476 462 775 Fragments arranged by size: 1476 775 602 462 BsrDI GCAATG_nn' Cuts at: 0 1031 1074 1280 3315 Size: 1031 43 206 2035 Fragments arranged by size: 2035 1031 206 43 BsrFI r'CCGG_y Cuts at: 0 919 1685 1695 3315 Size: 919 766 10 1620 Fragments arranged by size: 1620 919 766 10 BstXI CCAn_nnnn'nTGG Cuts at: 0 33 501 657 2281 3315 Size: 33 468 156 1624 1034 Fragments arranged by size: 1624 1034 468 156 33 BstYI r'GATC_y Cuts at: 0 233 3251 3315 Size: 233 3018 64 Fragments arranged by size: 3018 233 64 BstZ17I GTA'TAC Cuts at: 0 75 3315 Size: 75 3240 Bsu36I CC'TnA_GG Cuts at: 0 1656 2796 3315 Size: 1656 1140 519 Fragments arranged by size: 1656 1140 519 BtgI C'CryG_G Cuts at: 0 29 1088 1558 1640 2100 2280 2501 3206 Size: 29 1059 470 82 460 180 221 705 Cuts at: 3206 3315 Size: 109 Fragments arranged by size: 1059 705 470 460 221 180 109 82 29 BtsI GCAGTG_nn' Cuts at: 0 1089 2972 3315 Size: 1089 1883 343 Fragments arranged by size: 1883 1089 343 ClaI AT'CG_AT Cuts at: 0 290 1421 3315 Size: 290 1131 1894 Fragments arranged by size: 1894 1131 290 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2040 2075 3315 Size: 2040 35 1240 Fragments arranged by size: 2040 1240 35 DraIII CAC_nnn'GTG Cuts at: 0 340 3315 Size: 340 2975 DrdI GACnn_nn'nnGTC Cuts at: 0 1108 3315 Size: 1108 2207 EaeI y'GGCC_r Cuts at: 0 186 917 1142 1792 2927 3011 3315 Size: 186 731 225 650 1135 84 304 Fragments arranged by size: 1135 731 650 304 225 186 84 EarI CTCTTCn'nnn_ Cuts at: 0 244 268 710 1707 1754 2031 2046 3315 Size: 244 24 442 997 47 277 15 1269 Fragments arranged by size: 1269 997 442 277 244 47 24 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 544 679 731 895 1098 1118 1724 Size: 301 243 135 52 164 203 20 606 Cuts at: 1724 2089 2545 2590 2713 2789 3238 3315 Size: 365 456 45 123 76 449 77 Fragments arranged by size: 606 456 449 365 301 243 203 164 135 123 77 76 52 45 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 877 1759 3315 Size: 877 882 1556 Fragments arranged by size: 1556 882 877 EcoO109I rG'GnC_Cy Cuts at: 0 195 642 1026 1404 1524 1525 2580 2849 Size: 195 447 384 378 120 1 1055 269 Cuts at: 2849 3315 Size: 466 Fragments arranged by size: 1055 466 447 384 378 269 195 120 1 EcoRI G'AATT_C Cuts at: 0 1288 3315 Size: 1288 2027 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 255 3315 Size: 255 3060 FseI GG_CCGG'CC Cuts at: 0 923 3315 Size: 923 2392 HaeII r_GCGC'y Cuts at: 0 862 2256 2465 3315 Size: 862 1394 209 850 Fragments arranged by size: 1394 862 850 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 91 123 1140 1172 1182 1214 1342 1374 Size: 91 32 1017 32 10 32 128 32 Cuts at: 1374 1639 1671 1798 1828 1830 1860 2145 2177 Size: 265 32 127 30 2 30 285 32 Cuts at: 2177 3187 3219 3315 Size: 1010 32 96 Fragments arranged by size: 1017 1010 285 265 128 127 96 91 32 32 32 32 32 32 32 30 30 10 2 HincII GTy'rAC Cuts at: 0 384 2733 3315 Size: 384 2349 582 Fragments arranged by size: 2349 582 384 KasI G'GCGC_C Cuts at: 0 858 2252 3315 Size: 858 1394 1063 Fragments arranged by size: 1394 1063 858 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 548 713 1254 2399 2976 3315 Size: 548 165 541 1145 577 339 Fragments arranged by size: 1145 577 548 541 339 165 MscI TGG'CCA Cuts at: 0 188 1144 3315 Size: 188 956 2171 Fragments arranged by size: 2171 956 188 MslI CAynn'nnrTG Cuts at: 0 31 3076 3315 Size: 31 3045 239 Fragments arranged by size: 3045 239 31 MspA1I CmG'CkG Cuts at: 0 440 1138 1161 1560 1713 2102 2259 2503 Size: 440 698 23 399 153 389 157 244 Cuts at: 2503 2809 2986 3315 Size: 306 177 329 Fragments arranged by size: 698 440 399 389 329 306 244 177 157 153 23 NaeI GCC'GGC Cuts at: 0 921 1697 3315 Size: 921 776 1618 Fragments arranged by size: 1618 921 776 NarI GG'CG_CC Cuts at: 0 859 2253 3315 Size: 859 1394 1062 Fragments arranged by size: 1394 1062 859 NcoI C'CATG_G Cuts at: 0 3206 3315 Size: 3206 109 NdeI CA'TA_TG Cuts at: 0 1797 3315 Size: 1797 1518 NgoMIV G'CCGG_C Cuts at: 0 919 1695 3315 Size: 919 776 1620 Fragments arranged by size: 1620 919 776 NspI r_CATG'y Cuts at: 0 2459 3315 Size: 2459 856 PasI CC'CwG_GG Cuts at: 0 1125 3315 Size: 1125 2190 PflMI CCAn_nnn'nTGG Cuts at: 0 2595 3255 3315 Size: 2595 660 60 Fragments arranged by size: 2595 660 60 PfoI T'CCnGG_A Cuts at: 0 257 500 2477 3191 3254 3315 Size: 257 243 1977 714 63 61 Fragments arranged by size: 1977 714 257 243 63 61 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1957 1989 3315 Size: 1957 32 1326 Fragments arranged by size: 1957 1326 32 PpuMI rG'GwC_Cy Cuts at: 0 195 642 3315 Size: 195 447 2673 Fragments arranged by size: 2673 447 195 PspOMI G'GGCC_C Cuts at: 0 1163 1403 1524 1715 2579 3315 Size: 1163 240 121 191 864 736 Fragments arranged by size: 1163 864 736 240 191 121 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 121 153 3315 Size: 121 32 3162 Fragments arranged by size: 3162 121 32 PstI C_TGCA'G Cuts at: 0 152 1712 3315 Size: 152 1560 1603 Fragments arranged by size: 1603 1560 152 PvuII CAG'CTG Cuts at: 0 440 1138 2259 3315 Size: 440 698 1121 1056 Fragments arranged by size: 1121 1056 698 440 RsrII CG'GwC_CG Cuts at: 0 2181 3315 Size: 2181 1134 SacII CC_GC'GG Cuts at: 0 1561 2103 2504 3315 Size: 1561 542 401 811 Fragments arranged by size: 1561 811 542 401 SanDI GG'GwC_CC Cuts at: 0 642 3315 Size: 642 2673 ScaI AGT'ACT Cuts at: 0 1273 3315 Size: 1273 2042 SexAI A'CCwGG_T Cuts at: 0 91 3315 Size: 91 3224 SfcI C'TryA_G Cuts at: 0 148 1708 3315 Size: 148 1560 1607 Fragments arranged by size: 1607 1560 148 SfoI GGC'GCC Cuts at: 0 860 2254 3315 Size: 860 1394 1061 Fragments arranged by size: 1394 1061 860 SmaI CCC'GGG Cuts at: 0 2167 2778 3315 Size: 2167 611 537 Fragments arranged by size: 2167 611 537 SmlI C'TyrA_G Cuts at: 0 1567 1927 2290 3301 3315 Size: 1567 360 363 1011 14 Fragments arranged by size: 1567 1011 363 360 14 SnaBI TAC'GTA Cuts at: 0 72 3315 Size: 72 3243 StyI C'CwwG_G Cuts at: 0 884 1475 1508 2111 2657 2879 2999 3206 Size: 884 591 33 603 546 222 120 207 Cuts at: 3206 3315 Size: 109 Fragments arranged by size: 884 603 591 546 222 207 120 109 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1720 2169 2892 3273 3315 Size: 1720 449 723 381 42 Fragments arranged by size: 1720 723 449 381 42 TatI w'GTAC_w Cuts at: 0 1271 3315 Size: 1271 2044 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 808 840 2527 2559 3315 Size: 808 32 1687 32 756 Fragments arranged by size: 1687 808 756 32 32 Tth111I GACn'n_nGTC Cuts at: 0 93 346 3315 Size: 93 253 2969 Fragments arranged by size: 2969 253 93 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3006 3213 3256 3315 Size: 3006 207 43 59 Fragments arranged by size: 3006 207 59 43 XmaI C'CCGG_G Cuts at: 0 2165 2776 3315 Size: 2165 611 539 Fragments arranged by size: 2165 611 539 XmnI GAAnn'nnTTC Cuts at: 0 3271 3315 Size: 3271 44 Enzymes that do cut: AarI AccI AcuI AhdI AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII RsrII SacII SanDI ScaI SexAI SfcI SfoI SmaI SmlI SnaBI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmtI BplI BsiEI BsiWI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI DraI EagI EciI EcoICRI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PvuI SacI SalI SapI SbfI SfiI SgfI SgrAI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI ZraI