(Circular) (Six-base) MAPSORT of: pFN21AB7331.seq Check: 1591 from: 1 to: 7687 pFN21AB7331 7687 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2308 3381 4448 4756 2308 Size: 1073 1067 308 5239 Fragments arranged by size: 5239 1073 1067 308 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5497 278 Size: 53 83 186 936 3961 2468 Fragments arranged by size: 3961 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2835 4836 5030 1937 Size: 898 2001 194 4594 Fragments arranged by size: 4594 2001 898 194 Acc65I G'GTAC_C Cuts at: 2389 3659 5008 2389 Size: 1270 1349 5068 Fragments arranged by size: 5068 1349 1270 AclI AA'CG_TT Cuts at: 1563 5814 6187 1563 Size: 4251 373 3063 Fragments arranged by size: 4251 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2640 3191 3613 4640 5750 7134 7402 1632 Size: 1008 551 422 1027 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 1027 1008 551 422 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7490 AflIII A'CryG_T Cuts at: 1203 4130 6586 7563 1203 Size: 2927 2456 977 1327 Fragments arranged by size: 2927 2456 1327 977 AgeI A'CCGG_T Cuts at: 6518 6518 Size: 7687 AhdI GACnn_n'nnGTC Cuts at: 6416 6416 Size: 7687 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7687 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2507 3584 1184 Size: 1323 1077 5287 Fragments arranged by size: 5287 1323 1077 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2151 2183 2151 Size: 32 7655 AlwNI CAG_nnn'CTG Cuts at: 2544 2900 5430 7002 2544 Size: 356 2530 1572 3229 Fragments arranged by size: 3229 2530 1572 356 ApaI G_GGCC'C Cuts at: 2481 3999 4486 2481 Size: 1518 487 5682 Fragments arranged by size: 5682 1518 487 ApaLI G'TGCA_C Cuts at: 4190 5744 6900 4190 Size: 1554 1156 4977 Fragments arranged by size: 4977 1554 1156 ApoI r'AATT_y Cuts at: 1493 2228 2743 4996 5222 5374 7483 1493 Size: 735 515 2253 226 152 2109 1697 Fragments arranged by size: 2253 2109 1697 735 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 6241 160 Size: 872 5209 1606 Fragments arranged by size: 5209 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7687 AvaI C'yCGr_G Cuts at: 1870 1943 3304 3972 4061 5012 1870 Size: 73 1361 668 89 951 4545 Fragments arranged by size: 4545 1361 951 668 89 73 AvrII C'CTAG_G Cuts at: 2500 2500 Size: 7687 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3651 3684 3651 Size: 33 7654 BamHI G'GATC_C Cuts at: 2625 4241 5017 5384 2625 Size: 1616 776 367 4928 Fragments arranged by size: 4928 1616 776 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2389 3035 3176 3659 4114 Size: 325 217 627 602 646 141 483 455 Cuts at: 4114 4196 4544 4653 4744 5008 6463 618 Size: 82 348 109 91 264 1455 1842 Fragments arranged by size: 1842 1455 646 627 602 483 455 348 325 264 217 141 109 91 82 BanII G_rGCy'C Cuts at: 1391 2481 2765 3234 3531 3609 3710 3840 3999 Size: 1090 284 469 297 78 101 130 159 Cuts at: 3999 4172 4486 5006 1391 Size: 173 314 520 4072 Fragments arranged by size: 4072 1090 520 469 314 297 284 173 159 130 101 78 BbeI G_GCGC'C Cuts at: 3180 3180 Size: 7687 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6716 BbvCI CC'TCA_GC Cuts at: 2267 2786 2267 Size: 519 7168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3762 3796 5878 5912 1903 Size: 34 50 34 1741 34 2082 34 3678 Fragments arranged by size: 3678 2082 1741 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5579 6789 5579 Size: 1210 6477 BclI T'GATC_A Cuts at: 1548 1548 Size: 7687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2760 4458 6298 Size: 107 122 71 631 605 1088 1698 1840 Cuts at: 6298 136 Size: 1525 Fragments arranged by size: 1840 1698 1525 1088 631 605 122 107 71 BglII A'GATC_T Cuts at: 7682 7682 Size: 7687 BlpI GC'TnA_GC Cuts at: 3500 5104 3500 Size: 1604 6083 Bme1580I G_kGCm'C Cuts at: 1790 2481 2496 3142 3254 3999 4119 4194 4486 Size: 691 15 646 112 745 120 75 292 Cuts at: 4486 4534 4549 4606 4656 5748 6904 1790 Size: 48 15 57 50 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 745 691 646 292 120 112 75 57 50 48 15 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7687 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2398 3739 5418 Size: 416 53 83 399 24 981 1341 1679 Cuts at: 5418 6376 442 Size: 958 1753 Fragments arranged by size: 1753 1679 1341 981 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7687 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3724 3724 Size: 7687 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2358 2443 2457 2589 3279 3719 4347 4741 Size: 1119 85 14 132 690 440 628 394 Cuts at: 4741 4834 4841 6347 1239 Size: 93 7 1506 2579 Fragments arranged by size: 2579 1506 1119 690 628 440 394 132 93 85 14 7 Bpu10I CC'TnA_GC Cuts at: 2148 2267 2786 3068 4182 2148 Size: 119 519 282 1114 5653 Fragments arranged by size: 5653 1114 519 282 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 3365 4493 5812 6677 6975 2243 Size: 1122 1128 1319 865 298 2955 Fragments arranged by size: 2955 1319 1128 1122 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 4529 6350 882 Size: 617 1167 1863 1821 2219 Fragments arranged by size: 2219 1863 1821 1167 617 BsaAI yAC'GTr Cuts at: 493 1204 5478 493 Size: 711 4274 2702 Fragments arranged by size: 4274 2702 711 BsaBI GATnn'nnATC Cuts at: 2624 7681 2624 Size: 5057 2630 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 3177 4348 5494 Size: 53 83 186 936 633 1011 1171 1146 Cuts at: 5494 5876 6511 275 Size: 382 635 1451 Fragments arranged by size: 1451 1171 1146 1011 936 635 633 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2428 4135 6120 6518 6792 6939 7350 2428 Size: 1707 1985 398 274 147 411 2765 Fragments arranged by size: 2765 1985 1707 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 2774 2804 1178 Size: 30 165 30 1020 30 321 30 6061 Fragments arranged by size: 6061 1020 321 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2276 2635 2684 1186 Size: 940 150 359 49 6189 Fragments arranged by size: 6189 940 359 150 49 BseYI C'CCAG_C Cuts at: 2480 2534 3207 4075 4573 6890 7386 2480 Size: 54 673 868 498 2317 496 2781 Fragments arranged by size: 2781 2317 868 673 498 496 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2286 3359 3802 1163 Size: 573 550 1073 443 5048 Fragments arranged by size: 5048 1073 573 550 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 3847 5137 5898 6926 1540 Size: 275 186 1282 564 1290 761 1028 2301 Fragments arranged by size: 2301 1290 1282 1028 761 564 275 186 BsiHKAI G_wGCw'C Cuts at: 3531 4194 4341 5748 5833 6904 3531 Size: 663 147 1407 85 1071 4314 Fragments arranged by size: 4314 1407 1071 663 147 85 BsmI GAATG_Cn' Cuts at: 5198 5291 5198 Size: 93 7594 BsmBI CGTCTCn'nnnn_ Cuts at: 3295 4968 3295 Size: 1673 6014 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2481 2496 2765 3142 3234 3254 3531 Size: 399 691 15 269 377 92 20 277 Cuts at: 3531 3609 3710 3840 3999 4119 4172 4194 4341 Size: 78 101 130 159 120 53 22 147 Cuts at: 4341 4486 4534 4549 4606 4656 5006 5748 5833 Size: 145 48 15 57 50 350 742 85 Cuts at: 5833 6904 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 377 350 277 269 159 147 145 130 120 101 92 85 78 57 53 50 48 22 20 15 15 BspEI T'CCGG_A Cuts at: 2428 4135 7350 2428 Size: 1707 3215 2765 Fragments arranged by size: 3215 2765 1707 BspHI T'CATG_A Cuts at: 5576 7306 5576 Size: 1730 5957 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2308 3381 3489 4296 4448 4756 5042 844 Size: 1464 1073 108 807 152 308 286 3489 Fragments arranged by size: 3489 1464 1073 807 308 286 152 108 BsrBI CCG'CTC Cuts at: 1394 1823 5574 1394 Size: 429 3751 3507 Fragments arranged by size: 3751 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3274 3433 3595 4129 4779 6182 6356 Size: 1183 2026 159 162 534 650 1403 174 Cuts at: 6356 65 Size: 1396 Fragments arranged by size: 2026 1403 1396 1183 650 534 174 162 159 BsrFI r'CCGG_y Cuts at: 1700 1810 3022 3278 6331 6518 1700 Size: 110 1212 256 3053 187 2869 Fragments arranged by size: 3053 2869 1212 256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7687 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7687 BssSI C'ACGA_G Cuts at: 4597 5747 6759 4597 Size: 1150 1012 5525 Fragments arranged by size: 5525 1150 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4751 794 Size: 3957 3730 BstBI TT'CG_AA Cuts at: 2452 6491 2452 Size: 4039 3648 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7687 BstXI CCAn_nnnn'nTGG Cuts at: 4325 4765 4325 Size: 440 7247 BstYI r'GATC_y Cuts at: 1972 2625 4149 4241 4955 5017 5384 5769 5786 Size: 653 1524 92 714 62 367 385 17 Cuts at: 5786 7238 7324 7336 7682 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1524 1452 714 653 385 367 346 92 86 62 17 12 BstZ17I GTA'TAC Cuts at: 4837 4837 Size: 7687 Bsu36I CC'TnA_GG Cuts at: 2999 3354 2999 Size: 355 7332 BtgI C'CryG_G Cuts at: 513 1065 2082 2379 2585 4026 4200 4374 4519 Size: 552 1017 297 206 1441 174 174 145 Cuts at: 4519 513 Size: 3681 Fragments arranged by size: 3681 1441 1017 552 297 206 174 174 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4158 4826 487 Size: 683 56 2932 668 3348 Fragments arranged by size: 3348 2932 683 668 56 BtsI GCAGTG_nn' Cuts at: 2276 2496 2927 5203 5997 6017 2276 Size: 220 431 2276 794 20 3946 Fragments arranged by size: 3946 2276 794 431 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7687 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2785 2820 529 Size: 35 2221 35 5396 Fragments arranged by size: 5396 2221 35 35 DraI TTT'AAA Cuts at: 4991 4991 Size: 7687 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2424 6694 809 Size: 507 1108 4270 1802 Fragments arranged by size: 4270 1802 1108 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2937 3280 Size: 54 1425 50 275 18 504 603 343 Cuts at: 3280 3830 5134 6023 7652 8 Size: 550 1304 889 1629 43 Fragments arranged by size: 1629 1425 1304 889 603 550 504 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 5134 1537 Size: 275 1468 1854 4090 Fragments arranged by size: 4090 1854 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3876 4160 5617 1353 Size: 2523 284 1457 3423 Fragments arranged by size: 3423 2523 1457 284 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6274 6648 6794 387 Size: 1064 138 4685 374 146 1280 Fragments arranged by size: 4685 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3529 3529 Size: 7687 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2358 2443 2457 2589 2640 3191 3279 Size: 393 726 85 14 132 51 551 88 Cuts at: 3279 3613 3719 4347 4640 4741 4834 4841 5750 Size: 334 106 628 293 101 93 7 909 Cuts at: 5750 6347 7134 7402 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 909 787 726 628 597 551 393 334 293 268 132 106 101 93 88 85 51 14 7 EcoNI CCTnn'n_nnAGG Cuts at: 2237 3398 3701 2237 Size: 1161 303 6223 Fragments arranged by size: 6223 1161 303 EcoO109I rG'GnC_Cy Cuts at: 2074 2417 2477 2564 2729 2918 3445 3493 3926 Size: 343 60 87 165 189 527 48 433 Cuts at: 3926 3995 3996 4065 4267 4285 4483 2074 Size: 69 1 69 202 18 198 5278 Fragments arranged by size: 5278 527 433 343 202 198 189 165 87 69 69 60 48 18 1 EcoRI G'AATT_C Cuts at: 2228 4996 5374 2228 Size: 2768 378 4541 Fragments arranged by size: 4541 2768 378 FspI TGC'GCA Cuts at: 3920 6193 3920 Size: 2273 5414 HaeII r_GCGC'y Cuts at: 1678 2048 3180 6834 1678 Size: 370 1132 3654 2531 Fragments arranged by size: 3654 2531 1132 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 2588 2620 2774 Size: 32 135 32 851 32 133 32 154 Cuts at: 2774 2806 4233 4265 4277 4309 4940 4942 4972 Size: 32 1427 32 12 32 631 2 30 Cuts at: 4972 4974 6328 6360 6402 6434 1373 Size: 2 1354 32 42 32 2626 Fragments arranged by size: 2626 1427 1354 851 631 154 135 133 42 32 32 32 32 32 32 32 32 32 30 12 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3730 5031 5277 669 Size: 988 281 1792 1301 246 3079 Fragments arranged by size: 3079 1792 1301 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7687 HpaI GTT'AAC Cuts at: 5277 5277 Size: 7687 KasI G'GCGC_C Cuts at: 3176 3176 Size: 7687 KpnI G_GTAC'C Cuts at: 2393 3663 5012 2393 Size: 1270 1349 5068 Fragments arranged by size: 5068 1349 1270 MfeI C'AATT_G Cuts at: 5286 5286 Size: 7687 MluI A'CGCG_T Cuts at: 7563 7563 Size: 7687 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4035 4964 6801 6985 1305 Size: 2730 929 1837 184 2007 Fragments arranged by size: 2730 2007 1837 929 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2336 2939 3832 10 Size: 54 1425 343 504 603 893 3865 Fragments arranged by size: 3865 1425 893 603 504 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 3600 3911 4668 5645 6004 6163 Size: 648 1323 1111 311 757 977 359 159 Cuts at: 6163 518 Size: 2042 Fragments arranged by size: 2042 1323 1111 977 757 648 359 311 159 MspA1I CmG'CkG Cuts at: 1606 2218 3169 3364 3546 3554 3678 3786 4787 Size: 612 951 195 182 8 124 108 1001 Cuts at: 4787 4879 5103 5780 6928 7173 1606 Size: 92 224 677 1148 245 2120 Fragments arranged by size: 2120 1148 1001 951 677 612 245 224 195 182 124 108 92 8 NarI GG'CG_CC Cuts at: 3177 3177 Size: 7687 NcoI C'CATG_G Cuts at: 513 1065 2379 4026 4519 513 Size: 552 1314 1647 493 3681 Fragments arranged by size: 3681 1647 1314 552 493 NdeI CA'TA_TG Cuts at: 387 387 Size: 7687 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7687 NotI GC'GGCC_GC Cuts at: 5134 5134 Size: 7687 NspI r_CATG'y Cuts at: 1132 1229 3878 4134 5045 6590 1132 Size: 97 2649 256 911 1545 2229 Fragments arranged by size: 2649 2229 1545 911 256 97 PasI CC'CwG_GG Cuts at: 1348 2507 2608 3128 3254 1348 Size: 1159 101 520 126 5781 Fragments arranged by size: 5781 1159 520 126 101 PciI A'CATG_T Cuts at: 4130 6586 4130 Size: 2456 5231 PflMI CCAn_nnn'nTGG Cuts at: 1108 3026 3098 4161 4237 4520 1108 Size: 1918 72 1063 76 283 4275 Fragments arranged by size: 4275 1918 1063 283 76 72 PfoI T'CCnGG_A Cuts at: 2566 2880 4324 4497 2566 Size: 314 1444 173 5756 Fragments arranged by size: 5756 1444 314 173 PmeI GTTT'AAAC Cuts at: 4991 4991 Size: 7687 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5756 5788 7269 7301 5756 Size: 32 1481 32 6142 Fragments arranged by size: 6142 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2417 2564 4065 4267 4285 2417 Size: 147 1501 202 18 5819 Fragments arranged by size: 5819 1501 202 147 18 PshAI GACnn'nnGTC Cuts at: 1613 4131 1613 Size: 2518 5169 PsiI TTA'TAA Cuts at: 5257 5257 Size: 7687 PspOMI G'GGCC_C Cuts at: 2477 3995 4482 2477 Size: 1518 487 5682 Fragments arranged by size: 5682 1518 487 PstI C_TGCA'G Cuts at: 830 3559 4878 5039 830 Size: 2729 1319 161 3478 Fragments arranged by size: 3478 2729 1319 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7687 PvuII CAG'CTG Cuts at: 3169 3554 3678 4787 4879 3169 Size: 385 124 1109 92 5977 Fragments arranged by size: 5977 1109 385 124 92 SacI G_AGCT'C Cuts at: 3531 3531 Size: 7687 SalI G'TCGA_C Cuts at: 1936 5029 1936 Size: 3093 4594 SanDI GG'GwC_CC Cuts at: 2417 2417 Size: 7687 SapI GCTCTTCn'nnn_ Cuts at: 4160 4160 Size: 7687 SbfI CC_TGCA'GG Cuts at: 5039 5039 Size: 7687 ScaI AGT'ACT Cuts at: 3535 3989 5935 7647 3535 Size: 454 1946 1712 3575 Fragments arranged by size: 3575 1946 1712 454 SexAI A'CCwGG_T Cuts at: 4230 4230 Size: 7687 SfcI C'TryA_G Cuts at: 826 1045 2778 3313 3555 4687 4874 5035 6170 Size: 219 1733 535 242 1132 187 161 1135 Cuts at: 6170 6851 7042 826 Size: 681 191 1471 Fragments arranged by size: 1733 1471 1135 1132 681 535 242 219 191 187 161 SfoI GGC'GCC Cuts at: 3178 3178 Size: 7687 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7687 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7687 SmaI CCC'GGG Cuts at: 1872 3306 3974 4063 5014 1872 Size: 1434 668 89 951 4545 Fragments arranged by size: 4545 1434 951 668 89 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 3344 4508 5791 6692 6954 Size: 197 926 279 1122 1164 1283 901 262 Cuts at: 6954 820 Size: 1553 Fragments arranged by size: 1553 1283 1164 1122 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 5478 493 Size: 4985 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7687 SphI G_CATG'C Cuts at: 1132 5045 1132 Size: 3913 3774 SspI AAT'ATT Cuts at: 5 52 5611 5 Size: 47 5559 2081 Fragments arranged by size: 5559 2081 47 StuI AGG'CCT Cuts at: 1517 2910 3581 1517 Size: 1393 671 5623 Fragments arranged by size: 5623 1393 671 StyI C'CwwG_G Cuts at: 513 1065 2379 2409 2500 4026 4519 4740 5126 Size: 552 1314 30 91 1526 493 221 386 Cuts at: 5126 513 Size: 3074 Fragments arranged by size: 3074 1526 1314 552 493 386 221 91 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2999 3141 3221 3658 4587 4696 5714 Size: 1255 1419 142 80 437 929 109 1018 Cuts at: 5714 5731 5884 6069 6514 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1419 1255 1018 929 445 437 185 153 142 109 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3533 3987 5933 Size: 274 80 33 51 1443 1556 454 1946 Cuts at: 5933 7645 96 Size: 1712 138 Fragments arranged by size: 1946 1712 1556 1443 454 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2268 2300 2302 2334 7647 7679 2268 Size: 32 2 32 5313 32 2276 Fragments arranged by size: 5313 2276 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7687 XbaI T'CTAG_A Cuts at: 5023 5023 Size: 7687 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2564 3155 3652 4220 4372 2377 Size: 187 591 497 568 152 5692 Fragments arranged by size: 5692 591 568 497 187 152 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7687 XmaI C'CCGG_G Cuts at: 1870 3304 3972 4061 5012 1870 Size: 1434 668 89 951 4545 Fragments arranged by size: 4545 1434 951 668 89 XmnI GAAnn'nnTTC Cuts at: 5816 5816 Size: 7687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5495 276 Size: 53 83 186 936 3961 2468 Fragments arranged by size: 3961 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BsiWI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI