(Circular) (Six-base) MAPSORT of: pFN21AB8995.seq Check: 50 from: 1 to: 5941 pFN21AB8995 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2243 3284 1937 Size: 306 1041 4594 Fragments arranged by size: 4594 1041 306 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 3054 4840 5817 1203 Size: 1851 1786 977 1327 Fragments arranged by size: 1851 1786 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 3020 1166 Size: 1854 4087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2072 3038 1184 Size: 888 966 4087 Fragments arranged by size: 4087 966 888 AlwNI CAG_nnn'CTG Cuts at: 2965 3684 5256 2965 Size: 719 1572 3650 Fragments arranged by size: 3650 1572 719 ApaI G_GGCC'C Cuts at: 2045 2938 3132 2045 Size: 893 194 4854 Fragments arranged by size: 4854 893 194 ApaLI G'TGCA_C Cuts at: 3013 3998 5154 3013 Size: 985 1156 3800 Fragments arranged by size: 3800 1156 985 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2115 3172 3266 1870 Size: 73 172 1057 94 4545 Fragments arranged by size: 4545 1057 172 94 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3262 4717 618 Size: 325 217 627 1475 1455 1842 Fragments arranged by size: 1842 1475 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2938 3132 3173 3192 3260 1391 Size: 654 893 194 41 19 68 4072 Fragments arranged by size: 4072 893 654 194 68 41 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BbvCI CC'TCA_GC Cuts at: 2192 2192 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4132 4166 1903 Size: 34 50 34 2111 34 3678 Fragments arranged by size: 3678 2111 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2393 2453 2483 Size: 107 122 71 631 605 721 60 30 Cuts at: 2483 2513 2603 2663 2693 2723 2753 2783 2813 Size: 30 90 60 30 30 30 30 30 Cuts at: 2813 4552 136 Size: 1739 1525 Fragments arranged by size: 1739 1525 721 631 605 122 107 90 71 60 60 30 30 30 30 30 30 30 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2045 2418 2938 3017 3132 4002 5158 1790 Size: 255 373 520 79 115 870 1156 2573 Fragments arranged by size: 2573 1156 870 520 373 255 115 79 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2048 2232 2796 Size: 416 53 83 399 24 631 184 564 Cuts at: 2796 2921 3672 4630 442 Size: 125 751 958 1753 Fragments arranged by size: 1753 958 751 631 564 416 399 184 125 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 2591 4601 1239 Size: 927 425 2010 2579 Fragments arranged by size: 2579 2010 927 425 Bpu10I CC'TnA_GC Cuts at: 2192 2192 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3182 3748 4130 4765 Size: 53 83 186 936 1649 566 382 635 Cuts at: 4765 275 Size: 1451 Fragments arranged by size: 1649 1451 936 635 566 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2121 4374 4772 5046 5193 5604 2121 Size: 2253 398 274 147 411 2458 Fragments arranged by size: 2458 2253 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2164 2194 2283 2313 2774 Size: 30 165 30 761 30 89 30 461 Cuts at: 2774 2804 1178 Size: 30 4315 Fragments arranged by size: 4315 761 461 165 89 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 2205 1186 Size: 823 196 4922 Fragments arranged by size: 4922 823 196 BseYI C'CCAG_C Cuts at: 5144 5640 5144 Size: 496 5445 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2571 2601 2691 3209 1163 Size: 573 835 30 90 518 3895 Fragments arranged by size: 3895 835 573 518 90 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3017 4002 4087 5158 3017 Size: 985 85 1071 3800 Fragments arranged by size: 3800 1071 985 85 BsmI GAATG_Cn' Cuts at: 2889 3452 3545 2889 Size: 563 93 5285 Fragments arranged by size: 5285 563 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2418 2938 3017 3132 3173 3192 Size: 399 255 373 520 79 115 41 19 Cuts at: 3192 3260 4002 4087 5158 1391 Size: 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 399 373 255 115 85 79 68 41 19 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 2965 794 Size: 2171 3770 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2440 2500 2569 2689 2770 3088 2320 Size: 120 60 69 120 81 318 5173 Fragments arranged by size: 5173 318 120 120 81 69 60 BstYI r'GATC_y Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936 Size: 1299 367 385 17 1452 86 12 346 Cuts at: 5936 1972 Size: 1977 Fragments arranged by size: 1977 1452 1299 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2313 2343 2388 2418 2478 2538 2598 Size: 552 1248 30 45 30 60 60 60 Cuts at: 2598 2628 2658 2688 2718 2748 2778 2823 2895 Size: 30 30 30 30 30 30 45 72 Cuts at: 2895 513 Size: 3559 Fragments arranged by size: 3559 1248 552 72 60 60 60 45 45 30 30 30 30 30 30 30 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2583 2613 2703 2966 3457 4251 4271 2583 Size: 30 90 263 491 794 20 4253 Fragments arranged by size: 4253 794 491 263 90 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2475 2970 Size: 54 1425 50 275 18 492 153 495 Cuts at: 2970 2994 3388 4277 5906 8 Size: 24 394 889 1629 43 Fragments arranged by size: 1629 1425 889 495 492 394 275 153 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3871 1353 Size: 2518 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3023 4528 4902 5048 387 Size: 1064 138 1434 1505 374 146 1280 Fragments arranged by size: 1505 1434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2591 4004 4601 5388 5656 1239 Size: 393 534 425 1413 597 787 268 1524 Fragments arranged by size: 1524 1413 787 597 534 425 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3158 3158 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2042 2102 2785 2815 2934 2935 3128 3129 3151 Size: 60 683 30 119 1 193 1 22 Cuts at: 3151 2042 Size: 4832 Fragments arranged by size: 4832 683 193 119 60 30 22 1 1 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2990 5088 1678 Size: 1312 2098 2531 Fragments arranged by size: 2531 2098 1312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 2774 2804 2806 Size: 32 135 32 711 32 459 30 2 Cuts at: 2806 2836 2933 2965 4582 4614 4656 4688 1373 Size: 30 97 32 1617 32 42 32 2626 Fragments arranged by size: 2626 1617 711 459 135 97 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2920 3285 3531 669 Size: 988 281 982 365 246 3079 Fragments arranged by size: 3079 988 982 365 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 2972 2996 10 Size: 54 1425 343 492 648 24 2955 Fragments arranged by size: 2955 1425 648 492 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2687 2894 2951 3020 3086 3899 4258 Size: 648 1521 207 57 69 66 813 359 Cuts at: 4258 4417 518 Size: 159 2042 Fragments arranged by size: 2042 1521 813 648 359 207 159 69 66 57 MspA1I CmG'CkG Cuts at: 1606 2062 3357 4034 5182 5427 1606 Size: 456 1295 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 456 245 NcoI C'CATG_G Cuts at: 513 1065 2598 2628 2658 2688 2778 2823 2895 Size: 552 1533 30 30 30 90 45 72 Cuts at: 2895 513 Size: 3559 Fragments arranged by size: 3559 1533 552 90 72 45 30 30 30 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3058 3299 4844 1132 Size: 97 1829 241 1545 2229 Fragments arranged by size: 2229 1829 1545 241 97 PasI CC'CwG_GG Cuts at: 1348 3192 1348 Size: 1844 4097 PciI A'CATG_T Cuts at: 3054 4840 3054 Size: 1786 4155 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2102 2102 Size: 5941 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2041 2934 3128 2041 Size: 893 194 4854 Fragments arranged by size: 4854 893 194 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2062 2062 Size: 5941 RsrII CG'GwC_CG Cuts at: 3031 3031 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2102 2102 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 3289 4424 5105 5296 826 Size: 219 2244 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2117 3174 3268 1872 Size: 245 1057 94 4545 Fragments arranged by size: 4545 1057 245 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SrfI GCCC'GGGC Cuts at: 3174 3174 Size: 5941 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2568 1517 Size: 1051 4890 StyI C'CwwG_G Cuts at: 513 1065 2097 2598 2628 2658 2688 2778 2823 Size: 552 1032 501 30 30 30 90 45 Cuts at: 2823 2895 3159 3380 513 Size: 72 264 221 3074 Fragments arranged by size: 3074 1032 552 501 264 221 90 72 45 30 30 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4187 5899 96 Size: 274 80 33 51 1443 2210 1712 138 Fragments arranged by size: 2210 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 2980 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2115 3172 3266 1870 Size: 245 1057 94 4545 Fragments arranged by size: 4545 1057 245 94 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SapI SexAI SfiI SfoI SwaI