(Circular) (Six-base) MAPSORT of: pFN21AE0541.seq Check: 9595 from: 1 to: 5368 pFN21AE0541 5368 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3178 278 Size: 53 83 186 936 1642 2468 Fragments arranged by size: 2468 1642 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2711 1937 Size: 774 4594 Acc65I G'GTAC_C Cuts at: 2689 2689 Size: 5368 AclI AA'CG_TT Cuts at: 1563 3495 3868 1563 Size: 1932 373 3063 Fragments arranged by size: 3063 1932 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2150 3431 4815 5083 1632 Size: 518 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 518 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5171 AflIII A'CryG_T Cuts at: 1203 4267 5244 1203 Size: 3064 977 1327 Fragments arranged by size: 3064 1327 977 AgeI A'CCGG_T Cuts at: 2096 4199 2096 Size: 2103 3265 AhdI GACnn_n'nnGTC Cuts at: 4097 4097 Size: 5368 AleI CACnn'nnGTG Cuts at: 1166 2327 2507 1166 Size: 1161 180 4027 Fragments arranged by size: 4027 1161 180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2363 1184 Size: 1179 4189 AlwNI CAG_nnn'CTG Cuts at: 3111 4683 3111 Size: 1572 3796 ApaLI G'TGCA_C Cuts at: 3425 4581 3425 Size: 1156 4212 ApoI r'AATT_y Cuts at: 1493 2677 2903 3055 5164 1493 Size: 1184 226 152 2109 1697 Fragments arranged by size: 2109 1697 1184 226 152 AseI AT'TA_AT Cuts at: 160 1032 3922 160 Size: 872 2890 1606 Fragments arranged by size: 2890 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 AvaI C'yCGr_G Cuts at: 1870 1943 2693 1870 Size: 73 750 4545 Fragments arranged by size: 4545 750 73 BamHI G'GATC_C Cuts at: 2186 2253 2698 3065 2186 Size: 67 445 367 4489 Fragments arranged by size: 4489 445 367 67 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2277 2501 2689 4144 Size: 325 217 627 297 193 224 188 1455 Cuts at: 4144 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 297 224 217 193 188 BanII G_rGCy'C Cuts at: 1391 2109 2646 2687 1391 Size: 718 537 41 4072 Fragments arranged by size: 4072 718 537 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 928 Size: 971 516 3881 Fragments arranged by size: 3881 971 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3559 3593 1903 Size: 34 1622 34 3678 Fragments arranged by size: 3678 1622 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3260 4470 3260 Size: 1210 4158 BclI T'GATC_A Cuts at: 1548 1548 Size: 5368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3979 136 Size: 107 122 71 631 605 2307 1525 Fragments arranged by size: 2307 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5363 5363 Size: 5368 BlpI GC'TnA_GC Cuts at: 2785 2785 Size: 5368 Bme1580I G_kGCm'C Cuts at: 1790 2282 2504 3429 4585 1790 Size: 492 222 925 1156 2573 Fragments arranged by size: 2573 1156 925 492 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5368 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2473 2574 3099 Size: 416 53 83 399 24 1056 101 525 Cuts at: 3099 4057 442 Size: 958 1753 Fragments arranged by size: 1753 1056 958 525 416 399 101 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4028 1239 Size: 2789 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3493 4358 4656 3493 Size: 865 298 4205 Fragments arranged by size: 4205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4031 882 Size: 617 2532 2219 Fragments arranged by size: 2532 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2601 3159 493 Size: 711 1397 558 2702 Fragments arranged by size: 2702 1397 711 558 BsaBI GATnn'nnATC Cuts at: 5362 5362 Size: 5368 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2225 2235 3175 3557 Size: 53 83 186 936 692 10 940 382 Cuts at: 3557 4192 275 Size: 635 1451 Fragments arranged by size: 1451 940 936 692 635 382 186 83 53 10 BsaWI w'CCGG_w Cuts at: 2096 3801 4199 4473 4620 5031 2096 Size: 1705 398 274 147 411 2433 Fragments arranged by size: 2433 1705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 1178 Size: 30 165 30 858 30 4255 Fragments arranged by size: 4255 858 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5368 BseYI C'CCAG_C Cuts at: 4571 5067 4571 Size: 496 4872 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2333 1163 Size: 573 411 186 4198 Fragments arranged by size: 4198 573 411 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2818 3579 4607 1540 Size: 275 186 817 761 1028 2301 Fragments arranged by size: 2301 1028 817 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 3429 3514 4585 2109 Size: 1320 85 1071 2892 Fragments arranged by size: 2892 1320 1071 85 BsmI GAATG_Cn' Cuts at: 2879 2972 2879 Size: 93 5275 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2282 2504 2646 2687 3429 3514 Size: 399 319 173 222 142 41 742 85 Cuts at: 3514 4585 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 222 173 142 85 41 BspEI T'CCGG_A Cuts at: 5031 5031 Size: 5368 BspHI T'CATG_A Cuts at: 3257 4987 3257 Size: 1730 3638 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2123 2355 2642 2723 844 Size: 1279 232 287 81 3489 Fragments arranged by size: 3489 1279 287 232 81 BsrBI CCG'CTC Cuts at: 1394 1823 3255 1394 Size: 429 1432 3507 Fragments arranged by size: 3507 1432 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3863 4037 65 Size: 1183 2615 174 1396 Fragments arranged by size: 2615 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2096 2213 4012 4199 1700 Size: 110 228 58 117 1799 187 2869 Fragments arranged by size: 2869 1799 228 187 117 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5368 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5368 BssSI C'ACGA_G Cuts at: 2108 3428 4440 2108 Size: 1320 1012 3036 Fragments arranged by size: 3036 1320 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5368 BstBI TT'CG_AA Cuts at: 4172 4172 Size: 5368 BstEII G'GTnAC_C Cuts at: 1184 2209 1184 Size: 1025 4343 BstYI r'GATC_y Cuts at: 1972 2186 2253 2698 3065 3450 3467 4919 5005 Size: 214 67 445 367 385 17 1452 86 Cuts at: 5005 5017 5363 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 445 385 367 346 214 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2020 2257 2551 2569 2610 513 Size: 552 955 237 294 18 41 3271 Fragments arranged by size: 3271 955 552 294 237 41 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4629 Fragments arranged by size: 4629 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3678 3698 2884 Size: 794 20 4554 Fragments arranged by size: 4554 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5333 DraI TTT'AAA Cuts at: 2672 2672 Size: 5368 DraIII CAC_nnn'GTG Cuts at: 2155 2155 Size: 5368 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2119 4375 809 Size: 507 803 2256 1802 Fragments arranged by size: 2256 1802 803 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2197 2815 Size: 54 1425 50 275 18 206 161 618 Cuts at: 2815 3704 5333 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 618 275 206 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2815 1537 Size: 275 1003 4090 Fragments arranged by size: 4090 1003 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3298 1353 Size: 1945 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2266 3955 4329 4475 387 Size: 1064 138 677 1689 374 146 1280 Fragments arranged by size: 1689 1280 1064 677 374 146 138 EcoICRI GAG'CTC Cuts at: 2107 2107 Size: 5368 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 3431 4028 4815 5083 1239 Size: 393 518 1281 597 787 268 1524 Fragments arranged by size: 1524 1281 787 597 518 393 268 EcoO109I rG'GnC_Cy Cuts at: 2289 2289 Size: 5368 EcoRI G'AATT_C Cuts at: 2677 3055 2677 Size: 378 4990 FspI TGC'GCA Cuts at: 3874 3874 Size: 5368 HaeII r_GCGC'y Cuts at: 1678 2066 4515 1678 Size: 388 2449 2531 Fragments arranged by size: 2531 2449 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4009 4041 4083 4115 1373 Size: 32 135 32 2437 32 42 32 2626 Fragments arranged by size: 2626 2437 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2712 2958 669 Size: 988 281 774 246 3079 Fragments arranged by size: 3079 988 774 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5368 HpaI GTT'AAC Cuts at: 2958 2958 Size: 5368 KpnI G_GTAC'C Cuts at: 2693 2693 Size: 5368 MfeI C'AATT_G Cuts at: 2967 2967 Size: 5368 MluI A'CGCG_T Cuts at: 5244 5244 Size: 5368 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2403 4482 4666 1305 Size: 903 195 2079 184 2007 Fragments arranged by size: 2079 2007 903 195 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3546 Fragments arranged by size: 3546 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2507 3326 3685 3844 518 Size: 648 1161 180 819 359 159 2042 Fragments arranged by size: 2042 1161 819 648 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2022 2196 2259 2374 2784 3461 4609 4854 Size: 416 174 63 115 410 677 1148 245 Cuts at: 4854 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 416 410 245 174 115 63 NcoI C'CATG_G Cuts at: 513 1065 2610 513 Size: 552 1545 3271 Fragments arranged by size: 3271 1545 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5368 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5368 NotI GC'GGCC_GC Cuts at: 2815 2815 Size: 5368 NspI r_CATG'y Cuts at: 1132 1229 2330 2498 2578 2726 4271 1132 Size: 97 1101 168 80 148 1545 2229 Fragments arranged by size: 2229 1545 1101 168 148 97 80 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5368 PciI A'CATG_T Cuts at: 4267 4267 Size: 5368 PflMI CCAn_nnn'nTGG Cuts at: 1108 2497 1108 Size: 1389 3979 PmeI GTTT'AAAC Cuts at: 2672 2672 Size: 5368 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3437 3469 4950 4982 3437 Size: 32 1481 32 3823 Fragments arranged by size: 3823 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2289 2289 Size: 5368 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5368 PsiI TTA'TAA Cuts at: 2938 2938 Size: 5368 PstI C_TGCA'G Cuts at: 830 2653 2720 830 Size: 1823 67 3478 Fragments arranged by size: 3478 1823 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5368 SacI G_AGCT'C Cuts at: 2109 2109 Size: 5368 SacII CC_GC'GG Cuts at: 2023 2260 2023 Size: 237 5131 SalI G'TCGA_C Cuts at: 1936 2710 1936 Size: 774 4594 SanDI GG'GwC_CC Cuts at: 2289 2289 Size: 5368 SbfI CC_TGCA'GG Cuts at: 2720 2720 Size: 5368 ScaI AGT'ACT Cuts at: 3616 5328 3616 Size: 1712 3656 SfcI C'TryA_G Cuts at: 826 1045 2649 2716 3851 4532 4723 826 Size: 219 1604 67 1135 681 191 1471 Fragments arranged by size: 1604 1471 1135 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5368 SmaI CCC'GGG Cuts at: 1872 2695 1872 Size: 823 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3472 4373 4635 820 Size: 197 926 1529 901 262 1553 Fragments arranged by size: 1553 1529 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3159 493 Size: 2666 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5368 SphI G_CATG'C Cuts at: 1132 2578 2726 1132 Size: 1446 148 3774 Fragments arranged by size: 3774 1446 148 SspI AAT'ATT Cuts at: 5 52 3292 5 Size: 47 3240 2081 Fragments arranged by size: 3240 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5368 StyI C'CwwG_G Cuts at: 513 1065 2610 2807 513 Size: 552 1545 197 3074 Fragments arranged by size: 3074 1545 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3395 3412 3565 3750 4195 325 Size: 1255 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3614 5326 96 Size: 274 80 33 51 1443 1637 1712 138 Fragments arranged by size: 1712 1637 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2261 2293 5328 5360 2261 Size: 32 3035 32 2269 Fragments arranged by size: 3035 2269 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5368 XbaI T'CTAG_A Cuts at: 2704 2704 Size: 5368 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5368 XmaI C'CCGG_G Cuts at: 1870 2693 1870 Size: 823 4545 XmnI GAAnn'nnTTC Cuts at: 3497 3497 Size: 5368 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3176 276 Size: 53 83 186 936 1642 2468 Fragments arranged by size: 2468 1642 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI