Restriction map for
pFN21AE0363
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI BstBI
BstEII BstPI BstXI ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
FauI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MluI MluNI MroI MscI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 2013 GTATAC GT^[AC][GT]AC
AccII 66 CGCG CG^CG
AflIII 2100 ACATGT A^C[GA][CT]GT
AgeI 280 ACCGGT A^CCGGT
AlwNI 203 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 25 GAGCCC G[GA]GC[CT]^C
BclI 2073 TGATCA T^GATCA
BcnI 1117 CCCGG CC^[GC]GG
BlpI 2189 GCTTAGC GC^T[ACGT]AGC
BmyI 25 GAGCCC G[AGT]GC[ACT]^C
BpmI 1815 CTGGAG CTGGAG(16/14)
Bpu1102I 2189 GCTTAGC GC^T[ACGT]AGC
BsaBI 944 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 280 ACCGGT [AT]^CCGG[AT]
BsmI 872 GAATGC GAATGC(1/-1)
Bsp1286I 25 GAGCCC G[AGT]GC[ACT]^C
BspHI 138 TCATGA T^CATGA
BspLU11I 2100 ACATGT A^CATGT
BspMI 1044 ACCTGC ACCTGC(4/8)
BsrFI 280 ACCGGT [GA]^CCGG[CT]
Bst1107I 2013 GTATAC GTA^TAC
BstNI 29 CCTGG CC^[AT]GG
BstUI 66 CGCG CG^CG
BstYI 204 AGATCC [GA]^GATC[CT]
BstZ17I 2013 GTATAC GTA^TAC
CelII 2189 GCTTAGC GC^T[ACGT]AGC
Cfr10I 280 ACCGGT [GA]^CCGG[CT]
DraI 333 TTTAAA TTT^AAA
EcoRII 29 CCTGG ^CC[AT]GG
EcoRV 1810 GATATC GAT^ATC
EcoT22I 1326 ATGCAT ATGCA^T
FbaI 2073 TGATCA T^GATCA
HincII 1591 GTTGAC GT[CT]^[GA]AC
HindII 1591 GTTGAC GT[CT]^[GA]AC
HindIII 2187 AAGCTT A^AGCTT
HphI 1267 GGTGA GGTGA(8/7)
MamI 944 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1232 CAATTG C^AATTG
MflI 204 AGATCC [GA]^GATC[CT]
MslI 843 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1232 CAATTG C^AATTG
MvaI 29 CCTGG CC^[AT]GG
MvnI 66 CGCG CG^CG
NciI 1117 CCCGG CC^[GC]GG
NsiI 1326 ATGCAT ATGCA^T
PinAI 280 ACCGGT A^CCGGT
RcaI 138 TCATGA T^CATGA
SapI 1149 GCTCTTC GCTCTTC(1/4)
SfcI 2174 CTGTAG C^T[GA][CT]AG
SmlI 226 CTCAAG C^T[CT][GA]AG
Tsp45I 2097 GTCAC ^GT[GC]AC
XhoII 204 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 16 CCTCAGG CC^T[ACGT]AGG
1771 CCTGAGG
BbsI 276 GAAGAC GAAGAC(2/6)
905 GTCTTC
BpuAI 276 GAAGAC GAAGAC(2/6)
905 GTCTTC
BsaJI 28 CCCTGG C^C[ACGT][ACGT]GG
29 CCTGGG
BsmFI 32 GGGAC GGGAC(10/14)
1946 GGGAC
BssKI 29 CCTGG ^CC[ACGT]GG
1117 CCCGG
Bsu36I 16 CCTCAGG CC^T[ACGT]AGG
1771 CCTGAGG
CfoI 65 GCGC GCG^C
1416 GCGC
Eco81I 16 CCTCAGG CC^T[ACGT]AGG
1771 CCTGAGG
HapII 281 CCGG C^CGG
1118 CCGG
HhaI 65 GCGC GCG^C
1416 GCGC
HinP1I 65 GCGC G^CGC
1416 GCGC
HpaII 281 CCGG C^CGG
1118 CCGG
MspI 281 CCGG C^CGG
1118 CCGG
NspI 1332 ACATGC [GA]CATG^[CT]
2100 ACATGT
ScrFI 29 CCTGG CC^[ACGT]GG
1117 CCCGG
TaqI 342 TCGA T^CGA
1716 TCGA
TthHB8I 342 TCGA T^CGA
1716 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsaI
BseRI BsgI BsiYI BslI BsmAI
BsrDI BsrI BstAPI BstF5I Cac8I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MwoI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I SfaNI SspI TfiI Tru9I
TseI Tsp509I TspRI