(Circular) (Six-base) MAPSORT of: pFN21AE0363.seq Check: 46 from: 1 to: 6940 pFN21AE0363 6940 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4750 278 Size: 53 83 186 936 3214 2468 Fragments arranged by size: 3214 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4019 4283 1937 Size: 2082 264 4594 Fragments arranged by size: 4594 2082 264 Acc65I G'GTAC_C Cuts at: 4261 4261 Size: 6940 AclI AA'CG_TT Cuts at: 1563 5067 5440 1563 Size: 3504 373 3063 Fragments arranged by size: 3504 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2457 2621 2751 2919 3381 3915 5003 6387 Size: 825 164 130 168 462 534 1088 1384 Cuts at: 6387 6655 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1088 825 534 462 268 168 164 130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6743 AflIII A'CryG_T Cuts at: 1203 4105 5839 6816 1203 Size: 2902 1734 977 1327 Fragments arranged by size: 2902 1734 1327 977 AgeI A'CCGG_T Cuts at: 2285 5771 2285 Size: 3486 3454 AhdI GACnn_n'nnGTC Cuts at: 5669 5669 Size: 6940 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2325 1184 Size: 1141 5799 AlwNI CAG_nnn'CTG Cuts at: 2213 4683 6255 2213 Size: 2470 1572 2898 Fragments arranged by size: 2898 2470 1572 ApaLI G'TGCA_C Cuts at: 4997 6153 4997 Size: 1156 5784 ApoI r'AATT_y Cuts at: 1493 2531 3092 3356 3926 4249 4475 4627 6736 Size: 1038 561 264 570 323 226 152 2109 Cuts at: 6736 1493 Size: 1697 Fragments arranged by size: 2109 1697 1038 570 561 323 264 226 152 AseI AT'TA_AT Cuts at: 160 1032 5494 160 Size: 872 4462 1606 Fragments arranged by size: 4462 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 AvaI C'yCGr_G Cuts at: 1870 1943 4265 1870 Size: 73 2322 4545 Fragments arranged by size: 4545 2322 73 BamHI G'GATC_C Cuts at: 4270 4637 4270 Size: 367 6573 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4261 5716 618 Size: 325 217 627 2474 1455 1842 Fragments arranged by size: 2474 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 4259 1391 Size: 643 2225 4072 Fragments arranged by size: 4072 2225 643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 2903 928 Size: 971 389 615 4965 Fragments arranged by size: 4965 971 615 389 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5131 5165 1903 Size: 34 50 34 3110 34 3678 Fragments arranged by size: 3678 3110 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4832 6042 4832 Size: 1210 5730 BclI T'GATC_A Cuts at: 1548 4078 1548 Size: 2530 4410 BfrBI ATG'CAT Cuts at: 3333 3333 Size: 6940 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5551 136 Size: 107 122 71 631 605 3879 1525 Fragments arranged by size: 3879 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6935 6935 Size: 6940 BlpI GC'TnA_GC Cuts at: 4195 4357 4195 Size: 162 6778 Bme1580I G_kGCm'C Cuts at: 1790 5001 6157 1790 Size: 3211 1156 2573 Fragments arranged by size: 3211 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6940 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3016 3958 4671 Size: 416 53 83 399 24 1599 942 713 Cuts at: 4671 5629 442 Size: 958 1753 Fragments arranged by size: 1753 1599 958 942 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6940 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3841 5600 1239 Size: 2602 1759 2579 Fragments arranged by size: 2602 2579 1759 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2216 5065 5930 6228 2216 Size: 2849 865 298 2928 Fragments arranged by size: 2928 2849 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2589 2837 3384 4056 5603 882 Size: 617 1090 248 547 672 1547 2219 Fragments arranged by size: 2219 1547 1090 672 617 547 248 BsaAI yAC'GTr Cuts at: 493 1204 4731 493 Size: 711 3527 2702 Fragments arranged by size: 3527 2702 711 BsaBI GATnn'nnATC Cuts at: 2953 6934 2953 Size: 3981 2959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4747 5129 5764 275 Size: 53 83 186 936 3214 382 635 1451 Fragments arranged by size: 3214 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2285 5373 5771 6045 6192 6603 2285 Size: 3088 398 274 147 411 2622 Fragments arranged by size: 3088 2622 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2466 2496 4110 4140 1178 Size: 30 165 30 1063 30 1614 30 3978 Fragments arranged by size: 3978 1614 1063 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2010 2490 3525 3738 1186 Size: 824 480 1035 213 4388 Fragments arranged by size: 4388 1035 824 480 213 BseYI C'CCAG_C Cuts at: 2297 4200 6143 6639 2297 Size: 1903 1943 496 2598 Fragments arranged by size: 2598 1943 1903 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2199 2555 3246 3696 1163 Size: 573 463 356 691 450 4407 Fragments arranged by size: 4407 691 573 463 450 356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4390 5151 6179 1540 Size: 275 186 2389 761 1028 2301 Fragments arranged by size: 2389 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5001 5086 6157 5001 Size: 85 1071 5784 Fragments arranged by size: 5784 1071 85 BsmI GAATG_Cn' Cuts at: 2883 4451 4544 2883 Size: 1568 93 5279 Fragments arranged by size: 5279 1568 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 4259 5001 5086 6157 1391 Size: 399 244 2225 742 85 1071 2174 Fragments arranged by size: 2225 2174 1071 742 399 244 85 BspEI T'CCGG_A Cuts at: 6603 6603 Size: 6940 BspHI T'CATG_A Cuts at: 2143 4829 6559 2143 Size: 2686 1730 2524 Fragments arranged by size: 2686 2524 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3058 4295 844 Size: 2214 1237 3489 Fragments arranged by size: 3489 2214 1237 BsrBI CCG'CTC Cuts at: 1394 1823 4827 1394 Size: 429 3004 3507 Fragments arranged by size: 3507 3004 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 3925 4128 5435 5609 65 Size: 1183 1071 1606 203 1307 174 1396 Fragments arranged by size: 1606 1396 1307 1183 1071 203 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2285 5584 5771 1700 Size: 110 475 3299 187 2869 Fragments arranged by size: 3299 2869 475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6940 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6940 BssSI C'ACGA_G Cuts at: 5000 6012 5000 Size: 1012 5928 BstAPI GCAn_nnn'nTGC Cuts at: 794 2213 3330 4127 794 Size: 1419 1117 797 3607 Fragments arranged by size: 3607 1419 1117 797 BstBI TT'CG_AA Cuts at: 5744 5744 Size: 6940 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6940 BstYI r'GATC_y Cuts at: 1972 2209 4270 4637 5022 5039 6491 6577 6589 Size: 237 2061 367 385 17 1452 86 12 Cuts at: 6589 6935 1972 Size: 346 1977 Fragments arranged by size: 2061 1977 1452 385 367 346 237 86 17 12 BstZ17I GTA'TAC Cuts at: 4020 4020 Size: 6940 Bsu36I CC'TnA_GG Cuts at: 2022 3777 2022 Size: 1755 5185 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6388 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3343 487 Size: 683 56 2117 4084 Fragments arranged by size: 4084 2117 683 56 BtsI GCAGTG_nn' Cuts at: 3369 3756 4456 5250 5270 3369 Size: 387 700 794 20 5039 Fragments arranged by size: 5039 794 700 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6940 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6905 DraI TTT'AAA Cuts at: 2340 4244 2340 Size: 1904 5036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5947 809 Size: 507 4631 1802 Fragments arranged by size: 4631 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4387 5276 6905 Size: 54 1425 50 275 18 2557 889 1629 Cuts at: 6905 8 Size: 43 Fragments arranged by size: 2557 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4387 1537 Size: 275 2575 4090 Fragments arranged by size: 4090 2575 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2115 3161 3396 3891 4870 1353 Size: 762 1046 235 495 979 3423 Fragments arranged by size: 3423 1046 979 762 495 235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5527 5901 6047 387 Size: 1064 138 3938 374 146 1280 Fragments arranged by size: 3938 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2457 2621 2751 2919 3381 3841 3915 Size: 393 825 164 130 168 462 460 74 Cuts at: 3915 5003 5600 6387 6655 1239 Size: 1088 597 787 268 1524 Fragments arranged by size: 1524 1088 825 787 597 462 460 393 268 168 164 130 74 EcoRI G'AATT_C Cuts at: 4249 4627 4249 Size: 378 6562 EcoRV GAT'ATC Cuts at: 3817 3817 Size: 6940 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2888 3478 2888 Size: 590 6350 FspI TGC'GCA Cuts at: 5446 5446 Size: 6940 HaeII r_GCGC'y Cuts at: 1678 6087 1678 Size: 4409 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 5581 5613 5655 Size: 32 135 32 1187 32 2790 32 42 Cuts at: 5655 5687 1373 Size: 32 2626 Fragments arranged by size: 2790 2626 1187 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3598 4284 4530 669 Size: 988 281 1660 686 246 3079 Fragments arranged by size: 3079 1660 988 686 281 246 HindIII A'AGCT_T Cuts at: 748 4192 748 Size: 3444 3496 HpaI GTT'AAC Cuts at: 4530 4530 Size: 6940 KpnI G_GTAC'C Cuts at: 4265 4265 Size: 6940 MfeI C'AATT_G Cuts at: 3237 4539 3237 Size: 1302 5638 MluI A'CGCG_T Cuts at: 6816 6816 Size: 6940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6054 6238 1305 Size: 4749 184 2007 Fragments arranged by size: 4749 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5118 Fragments arranged by size: 5118 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2852 4898 5257 5416 518 Size: 648 1686 2046 359 159 2042 Fragments arranged by size: 2046 2042 1686 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2079 4015 4356 5033 6181 6426 1606 Size: 455 18 1936 341 677 1148 245 2120 Fragments arranged by size: 2120 1936 1148 677 455 341 245 18 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6388 NdeI CA'TA_TG Cuts at: 387 2779 3990 3999 387 Size: 2392 1211 9 3328 Fragments arranged by size: 3328 2392 1211 9 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6940 NotI GC'GGCC_GC Cuts at: 4387 4387 Size: 6940 NsiI A_TGCA'T Cuts at: 3335 3335 Size: 6940 NspI r_CATG'y Cuts at: 1132 1229 3341 4109 4298 5843 1132 Size: 97 2112 768 189 1545 2229 Fragments arranged by size: 2229 2112 1545 768 189 97 PasI CC'CwG_GG Cuts at: 1348 2034 1348 Size: 686 6254 PciI A'CATG_T Cuts at: 4105 5839 4105 Size: 1734 5206 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6940 PmeI GTTT'AAAC Cuts at: 4244 4244 Size: 6940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2340 2372 5009 5041 6522 6554 2340 Size: 32 2637 32 1481 32 2726 Fragments arranged by size: 2726 2637 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6940 PsiI TTA'TAA Cuts at: 4510 4510 Size: 6940 PstI C_TGCA'G Cuts at: 830 4292 830 Size: 3462 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6940 SalI G'TCGA_C Cuts at: 1936 4282 1936 Size: 2346 4594 SapI GCTCTTCn'nnn_ Cuts at: 3161 3161 Size: 6940 SbfI CC_TGCA'GG Cuts at: 4292 4292 Size: 6940 ScaI AGT'ACT Cuts at: 5188 6900 5188 Size: 1712 5228 SfcI C'TryA_G Cuts at: 826 1045 4179 4288 5423 6104 6295 826 Size: 219 3134 109 1135 681 191 1471 Fragments arranged by size: 3134 1471 1135 681 219 191 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6940 SmaI CCC'GGG Cuts at: 1872 4267 1872 Size: 2395 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2231 5044 5945 6207 820 Size: 197 926 288 2813 901 262 1553 Fragments arranged by size: 2813 1553 926 901 288 262 197 SnaBI TAC'GTA Cuts at: 493 4731 493 Size: 4238 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6940 SphI G_CATG'C Cuts at: 1132 4298 1132 Size: 3166 3774 SspI AAT'ATT Cuts at: 5 52 3001 3414 3649 3958 4035 4864 5 Size: 47 2949 413 235 309 77 829 2081 Fragments arranged by size: 2949 2081 829 413 309 235 77 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6940 StyI C'CwwG_G Cuts at: 513 1065 4379 513 Size: 552 3314 3074 Fragments arranged by size: 3314 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2055 4967 4984 5137 5322 5767 325 Size: 1255 475 2912 17 153 185 445 1498 Fragments arranged by size: 2912 1498 1255 475 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 5186 6898 Size: 274 80 33 51 1443 471 2738 1712 Cuts at: 6898 96 Size: 138 Fragments arranged by size: 2738 1712 1443 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6900 6932 6900 Size: 32 6908 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6940 XbaI T'CTAG_A Cuts at: 4276 4276 Size: 6940 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6940 XmaI C'CCGG_G Cuts at: 1870 4265 1870 Size: 2395 4545 XmnI GAAnn'nnTTC Cuts at: 5069 5069 Size: 6940 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4748 276 Size: 53 83 186 936 3214 2468 Fragments arranged by size: 3214 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI