################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 211 AAATTT [GA]^AATT[CT] ApaI 203 GGGCCC GGGCC^C ApoI 211 AAATTT [GA]^AATT[CT] AscI 164 GGCGCGCC GG^CGCGCC BanII 203 GGGCCC G[GA]GC[CT]^C BlpI 267 GCTGAGC GC^T[ACGT]AGC BmyI 203 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 267 GCTGAGC GC^T[ACGT]AGC BsaI 186 GGTCTC GGTCTC(1/5) BseRI 262 GAGGAG GAGGAG(10/8) BsiWI 155 CGTACG C^GTACG BsmAI 187 GTCTC GTCTC(1/5) Bsp1286I 203 GGGCCC G[AGT]GC[ACT]^C BsrBI 160 GAGCGG CCGCTC(-3/-3) BsrI 279 ACTGG ACTGG(1/-1) BssHII 165 GCGCGC G^CGCGC BssKI 71 CCAGG ^CC[ACGT]GG BstF5I 240 CATCC GGATG(2/0) BstNI 71 CCAGG CC^[AT]GG CelII 267 GCTGAGC GC^T[ACGT]AGC DdeI 268 CTGAG C^T[ACGT]AG DraII 203 GGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 43 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 30 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 203 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 71 CCAGG ^CC[AT]GG FokI 240 CATCC GGATG(9/13) HinfI 129 GATTC G^A[ACGT]TC KspI 87 CCGCGG CCGC^GG MspA1I 87 CCGCGG C[AC]G^C[GT]G MvaI 71 CCAGG CC^[AT]GG NcoI 170 CCATGG C^CATGG NlaIV 203 GGGCCC GG[ACGT]^[ACGT]CC SacII 87 CCGCGG CCGC^GG ScrFI 71 CCAGG CC^[ACGT]GG TfiI 129 GATTC G^A[AT]TC Tsp45I 49 GTGAC ^GT[GC]AC Tsp509I 212 AATT ^AATT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 83 GACGCC G[GA]^CG[CT]C 92 GGCGTC AfaI 156 GTAC GT^AC 277 GTAC AluI 266 AGCT AG^CT 297 AGCT BbvI 298 GCTGC GCAGC(8/12) 301 GCTGC BsaHI 83 GACGCC G[GA]^CG[CT]C 92 GGCGTC BsiYI 30 CCTCAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 284 CCGCCATGAGG BslI 30 CCTCAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 284 CCGCCATGAGG BsmFI 122 GGGAC GGGAC(10/14) 232 GGGAC EaeI 20 TGGCCG [CT]^GGCC[GA] 281 TGGCCG EcoT14I 170 CCATGG C^C[AT][AT]GG 191 CCAAGG Hin1I 83 GACGCC G[GA]^CG[CT]C 92 GGCGTC MaeII 59 ACGT A^CGT 275 ACGT MaeIII 49 GTGAC ^GT[ACGT]AC 177 GTTAC MwoI 80 GCCGACGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 258 GCGGGAGGAGC RsaI 156 GTAC GT^AC 277 GTAC StyI 170 CCATGG C^C[AT][AT]GG 191 CCAAGG TseI 298 GCTGC G^C[AT]GC 301 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AccII AciI BsaJI BstUI Cac8I CfoI Cfr13I DsaI FauI Fnu4HI HaeIII HgaI HhaI HinP1I ItaI MnlI MvnI NlaIII Sau96I