Restriction map for
pFN21AE0539
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# NO CUT ENZYMES ( 103 enzymes) #
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AclI AcsI AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI AspEI AvrII
BbeI BbrPI BbsI BbvCI BfaI
BglI BglII BlnI BpuAI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI HaeII HgaI HindIII HpaI
KasI KspI MaeI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AatII 652 GACGTC GACGT^C
Acc65I 612 GGTACC G^GTACC
AccI 178 GTCGAC GT^[AC][GT]AC
AccIII 296 TCCGGA T^CCGGA
AcyI 652 GACGTC G[GA]^CG[CT]C
AflII 1093 CTTAAG C^TTAAG
AocI 97 CCTAAGG CC^T[ACGT]AGG
ApaI 587 GGGCCC GGGCC^C
Asp718I 612 GGTACC G^GTACC
AspI 985 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 287 CTCGAG C^[CT]CG[GA]G
AviII 743 TGCGCA TGC^GCA
BamHI 190 GGATCC G^GATCC
BanI 612 GGTACC G^G[CT][GA]CC
BcgI 771 GCATCCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 142 GGATAC GTATCC(6/5)
BclI 893 TGATCA T^GATCA
BcnI 187 CCGGG CC^[GC]GG
BfrI 1093 CTTAAG C^TTAAG
BlpI 319 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 319 GCTGAGC GC^T[ACGT]AGC
BsaAI 274 TACGTG [CT]AC^GT[GA]
BsaBI 540 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 652 GACGTC G[GA]^CG[CT]C
BsaWI 296 TCCGGA [AT]^CCGG[AT]
BseAI 296 TCCGGA T^CCGGA
BseRI 1147 GAGGAG GAGGAG(10/8)
BsgI 1057 GTGCAG GTGCAG(16/14)
BsoBI 287 CTCGAG C^[CT]CG[GA]G
BspEI 296 TCCGGA T^CCGGA
BspMI 609 GCAGGT ACCTGC(4/8)
BsrBI 20 CCGCTC CCGCTC(-3/-3)
BsrDI 486 CATTGC GCAATG(2/0)
BsrFI 117 GCCGGT [GA]^CCGG[CT]
BssSI 779 CTCGTG CACGAG(-5/-1)
BstXI 624 CCAAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 97 CCTAAGG CC^T[ACGT]AGG
CelII 319 GCTGAGC GC^T[ACGT]AGC
Cfr10I 117 GCCGGT [GA]^CCGG[CT]
DraII 34 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 163 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 87 GAAGAG CTCTTC(1/4)
Eco81I 97 CCTAAGG CC^T[ACGT]AGG
EcoO109I 34 GGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 893 TGATCA T^GATCA
FspI 743 TGCGCA TGC^GCA
Hin1I 652 GACGTC G[GA]^CG[CT]C
HincII 178 GTCGAC GT[CT]^[GA]AC
HindII 178 GTCGAC GT[CT]^[GA]AC
HinfI 159 GACTC G^A[ACGT]TC
KpnI 612 GGTACC GGTAC^C
Ksp632I 87 GAAGAG CTCTTC(1/4)
MamI 540 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 296 TCCGGA T^CCGGA
MseI 1094 TTAA T^TAA
MspA1I 433 CAGCTG C[AC]G^C[GT]G
NciI 187 CCGGG CC^[GC]GG
PaeR7I 287 CTCGAG C^TCGAG
PflFI 985 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 159 GACTC GAGTC(4/5)
PpuMI 34 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 29 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 433 CAGCTG CAG^CTG
SalI 178 GTCGAC G^TCGAC
SapI 87 GAAGAGC GCTCTTC(1/4)
Tru9I 1094 TTAA T^TAA
Tth111I 985 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 248 CCATGGTGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 287 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 592 CGCG CG^CG
802 CGCG
AspHI 322 GAGCAC G[AT]GC[AT]^C
1030 GTGCTC
BalI 861 TGGCCA TGG^CCA
1122 TGGCCA
BanII 587 GGGCCC G[GA]GC[CT]^C
1073 GGGCTC
BpmI 436 CTGGAG CTGGAG(16/14)
1078 CTGGAG
BsiHKAI 322 GAGCAC G[AT]GC[AT]^C
1030 GTGCTC
BsmAI 238 GAGAC GTCTC(1/5)
3 GTCTC
BsmFI 1167 GGGAC GGGAC(10/14)
36 GTCCC
BsmI 337 GAATGC GAATGC(1/-1)
1159 GCATTC
BsrI 584 ACTGG ACTGG(1/-1)
695 CCAGT
BstEII 663 GGTGACC G^GT[ACGT]ACC
690 GGTCACC
BstPI 663 GGTGACC G^GT[ACGT]ACC
690 GGTCACC
BstUI 592 CGCG CG^CG
802 CGCG
BstYI 190 GGATCC [GA]^GATC[CT]
545 AGATCC
EcoNI 741 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
873 CCTTATCAAGG
EcoO65I 663 GGTGACC G^GT[ACGT]ACC
690 GGTCACC
MaeII 275 ACGT A^CGT
653 ACGT
MflI 190 GGATCC [GA]^GATC[CT]
545 AGATCC
MluNI 861 TGGCCA TGG^CCA
1122 TGGCCA
MscI 861 TGGCCA TGG^CCA
1122 TGGCCA
MvnI 592 CGCG CG^CG
802 CGCG
ScaI 395 AGTACT AGT^ACT
464 AGTACT
Tsp509I 361 AATT ^AATT
1188 AATT
XhoII 190 GGATCC [GA]^GATC[CT]
545 AGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AvaII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XmnI