(Circular) (Six-base) MAPSORT of: pFN21AE0019.seq Check: 5505 from: 1 to: 6481 pFN21AE0019 6481 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3824 1937 Size: 1887 4594 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2940 4544 5928 6196 1632 Size: 1308 1604 1384 268 1917 Fragments arranged by size: 1917 1604 1384 1308 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3044 1184 Size: 1860 4621 AlwNI CAG_nnn'CTG Cuts at: 4224 5796 4224 Size: 1572 4909 ApaI G_GGCC'C Cuts at: 2281 2281 Size: 6481 ApaLI G'TGCA_C Cuts at: 3633 4538 5694 3633 Size: 905 1156 4420 Fragments arranged by size: 4420 1156 905 ApoI r'AATT_y Cuts at: 1493 2930 3790 4016 4168 6277 1493 Size: 1437 860 226 152 2109 1697 Fragments arranged by size: 2109 1697 1437 860 226 152 AseI AT'TA_AT Cuts at: 160 1032 3108 5035 160 Size: 872 2076 1927 1606 Fragments arranged by size: 2076 1927 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 3806 1870 Size: 73 1863 4545 Fragments arranged by size: 4545 1863 73 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3802 5257 618 Size: 325 217 627 2015 1455 1842 Fragments arranged by size: 2015 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2281 3369 3800 1391 Size: 890 1088 431 4072 Fragments arranged by size: 4072 1088 890 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3267 928 Size: 971 1368 4142 Fragments arranged by size: 4142 1368 971 BbvCI CC'TCA_GC Cuts at: 3013 3013 Size: 6481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4672 4706 1903 Size: 34 2735 34 3678 Fragments arranged by size: 3678 2735 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5583 4373 Size: 1210 5271 BclI T'GATC_A Cuts at: 1548 3702 1548 Size: 2154 4327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6476 6476 Size: 6481 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 2281 3637 4542 5698 1790 Size: 491 1356 905 1156 2573 Fragments arranged by size: 2573 1356 1156 905 491 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2233 3736 4212 Size: 416 53 83 399 24 816 1503 476 Cuts at: 4212 5170 442 Size: 958 1753 Fragments arranged by size: 1753 1503 958 816 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3076 3076 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 3588 5141 1239 Size: 1337 1012 1553 2579 Fragments arranged by size: 2579 1553 1337 1012 Bpu10I CC'TnA_GC Cuts at: 3013 3013 Size: 6481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 4606 5471 5769 2141 Size: 2465 865 298 2853 Fragments arranged by size: 2853 2465 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2392 2900 5144 882 Size: 617 893 508 2244 2219 Fragments arranged by size: 2244 2219 893 617 508 BsaAI yAC'GTr Cuts at: 493 1204 4272 493 Size: 711 3068 2702 Fragments arranged by size: 3068 2702 711 BsaBI GATnn'nnATC Cuts at: 6475 6475 Size: 6481 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4914 5312 5586 5733 6144 4914 Size: 398 274 147 411 5251 Fragments arranged by size: 5251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6256 Fragments arranged by size: 6256 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2937 2940 1186 Size: 1751 3 4727 Fragments arranged by size: 4727 1751 3 BseYI C'CCAG_C Cuts at: 2640 5684 6180 2640 Size: 3044 496 2941 Fragments arranged by size: 3044 2941 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3062 3321 3638 1163 Size: 573 1326 259 317 4006 Fragments arranged by size: 4006 1326 573 317 259 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3057 3637 4542 4627 5698 3057 Size: 580 905 85 1071 3840 Fragments arranged by size: 3840 1071 905 580 85 BsmI GAATG_Cn' Cuts at: 3992 4085 3992 Size: 93 6388 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2281 3057 3369 3637 3800 4542 4627 Size: 399 491 776 312 268 163 742 85 Cuts at: 4627 5698 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 776 742 491 399 312 268 163 85 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3015 3660 3836 844 Size: 2171 645 176 3489 Fragments arranged by size: 3489 2171 645 176 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4976 5150 65 Size: 1183 3728 174 1396 Fragments arranged by size: 3728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5125 5312 1700 Size: 110 3315 187 2869 Fragments arranged by size: 3315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2341 96 Size: 2245 4236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 3411 794 Size: 2617 3864 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 2698 3811 4178 4563 4580 6032 6118 6130 Size: 726 1113 367 385 17 1452 86 12 Cuts at: 6130 6476 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1113 726 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3673 513 Size: 552 2608 3321 Fragments arranged by size: 3321 2608 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5742 Fragments arranged by size: 5742 683 56 BtsI GCAGTG_nn' Cuts at: 3309 3997 4791 4811 3309 Size: 688 794 20 4979 Fragments arranged by size: 4979 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 2502 3785 2502 Size: 1283 5198 DraIII CAC_nnn'GTG Cuts at: 3632 3632 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2326 3384 3928 Size: 54 1425 50 275 18 496 1058 544 Cuts at: 3928 4817 6446 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1058 889 544 496 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3045 4411 1353 Size: 1692 1366 3423 Fragments arranged by size: 3423 1692 1366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 2940 3588 4544 5141 5928 6196 Size: 393 944 364 648 956 597 787 268 Cuts at: 6196 1239 Size: 1524 Fragments arranged by size: 1524 956 944 787 648 597 393 364 268 EcoO109I rG'GnC_Cy Cuts at: 2277 2277 Size: 6481 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 5628 1678 Size: 3950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2353 2385 2996 3028 5122 Size: 32 135 32 781 32 611 32 2094 Cuts at: 5122 5154 5196 5228 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2094 781 611 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3825 4071 669 Size: 988 281 1887 246 3079 Fragments arranged by size: 3079 1887 988 281 246 HindIII A'AGCT_T Cuts at: 748 3514 748 Size: 2766 3715 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2051 2230 3404 3552 5595 5779 1305 Size: 746 179 1174 148 2043 184 2007 Fragments arranged by size: 2043 2007 1174 746 184 179 148 MscI TGG'CCA Cuts at: 10 64 1489 1832 2328 3386 10 Size: 54 1425 343 496 1058 3105 Fragments arranged by size: 3105 1425 1058 496 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2190 3695 4439 4798 4957 518 Size: 648 1024 1505 744 359 159 2042 Fragments arranged by size: 2042 1505 1024 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2339 3017 3897 4574 5722 5967 1606 Size: 733 678 880 677 1148 245 2120 Fragments arranged by size: 2120 1148 880 733 678 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5929 NdeI CA'TA_TG Cuts at: 387 3228 387 Size: 2841 3640 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 3031 3599 3839 5384 1132 Size: 97 1802 568 240 1545 2229 Fragments arranged by size: 2229 1802 1545 568 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6481 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 4051 4051 Size: 6481 PspOMI G'GGCC_C Cuts at: 2277 2277 Size: 6481 PstI C_TGCA'G Cuts at: 830 3560 3833 830 Size: 2730 273 3478 Fragments arranged by size: 3478 2730 273 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2339 3017 2339 Size: 678 5803 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SapI GCTCTTCn'nnn_ Cuts at: 3045 3045 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 3185 3556 3829 4964 5645 5836 826 Size: 219 2140 371 273 1135 681 191 1471 Fragments arranged by size: 2140 1471 1135 681 371 273 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 4585 5486 5748 820 Size: 197 926 177 2465 901 262 1553 Fragments arranged by size: 2465 1553 926 901 262 197 177 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3031 3839 1132 Size: 1899 808 3774 Fragments arranged by size: 3774 1899 808 SspI AAT'ATT Cuts at: 5 52 2170 3497 4405 5 Size: 47 2118 1327 908 2081 Fragments arranged by size: 2118 2081 1327 908 47 StuI AGG'CCT Cuts at: 1517 3038 1517 Size: 1521 4960 StyI C'CwwG_G Cuts at: 513 1065 3920 513 Size: 552 2855 3074 Fragments arranged by size: 3074 2855 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4508 4525 4678 4863 5308 325 Size: 1255 2928 17 153 185 445 1498 Fragments arranged by size: 2928 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2341 3251 4727 Size: 274 80 33 51 1443 364 910 1476 Cuts at: 4727 6439 96 Size: 1712 138 Fragments arranged by size: 1712 1476 1443 910 364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 2944 3437 3817 2944 Size: 493 380 5608 Fragments arranged by size: 5608 493 380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2246 2648 3094 2246 Size: 402 446 5633 Fragments arranged by size: 5633 446 402 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 2202 4610 2202 Size: 2408 4073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI