(Circular) (Six-base) MAPSORT of: pFN21AB7680.seq Check: 2604 from: 1 to: 5923 pFN21AB7680 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 3986 5370 5638 1632 Size: 557 1797 1384 268 1917 Fragments arranged by size: 1917 1797 1384 557 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 2208 2597 3666 5238 2208 Size: 389 1069 1572 2893 Fragments arranged by size: 2893 1572 1069 389 ApaI G_GGCC'C Cuts at: 2464 2741 2761 2464 Size: 277 20 5626 Fragments arranged by size: 5626 277 20 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 3164 3232 3458 3610 5719 1493 Size: 1671 68 226 152 2109 1697 Fragments arranged by size: 2109 1697 1671 226 152 68 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2531 2918 3248 1870 Size: 73 588 387 330 4545 Fragments arranged by size: 4545 588 387 330 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2474 2507 2474 Size: 33 5890 BamHI G'GATC_C Cuts at: 3214 3253 3620 3214 Size: 39 367 5517 Fragments arranged by size: 5517 367 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2681 2969 3017 3244 4699 Size: 325 217 627 894 288 48 227 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 894 627 325 288 227 217 48 BanII G_rGCy'C Cuts at: 1391 2420 2464 2532 2567 2741 2761 2796 3242 Size: 1029 44 68 35 174 20 35 446 Cuts at: 3242 1391 Size: 4072 Fragments arranged by size: 4072 1029 446 174 68 44 35 35 20 BbeI G_GCGC'C Cuts at: 2973 2973 Size: 5923 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2700 2864 928 Size: 971 801 164 3987 Fragments arranged by size: 3987 971 801 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2899 2933 4114 4148 1903 Size: 34 962 34 1181 34 3678 Fragments arranged by size: 3678 1181 962 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2956 3066 4534 Size: 107 122 71 631 605 1284 110 1468 Cuts at: 4534 136 Size: 1525 Fragments arranged by size: 1525 1468 1284 631 605 122 110 107 71 BglII A'GATC_T Cuts at: 2331 5918 2331 Size: 3587 2336 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2150 2464 2741 2761 3984 5140 1790 Size: 360 314 277 20 1223 1156 2573 Fragments arranged by size: 2573 1223 1156 360 314 277 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 3654 4612 Size: 416 53 83 399 24 1457 780 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1457 958 780 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2404 2982 3083 3126 3167 3209 4583 1239 Size: 1165 578 101 43 41 42 1374 2579 Fragments arranged by size: 2579 1374 1165 578 101 43 42 41 Bpu10I CC'TnA_GC Cuts at: 2387 2387 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2970 3069 3730 Size: 53 83 186 936 479 958 99 661 Cuts at: 3730 4112 4747 275 Size: 382 635 1451 Fragments arranged by size: 1451 958 936 661 635 479 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 2070 2981 4356 4754 5028 5175 5586 2070 Size: 911 1375 398 274 147 411 2407 Fragments arranged by size: 2407 1375 911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2544 2574 2873 2903 1178 Size: 30 165 30 1141 30 299 30 4198 Fragments arranged by size: 4198 1141 299 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 3039 3056 1186 Size: 1258 595 17 4053 Fragments arranged by size: 4053 1258 595 17 BseYI C'CCAG_C Cuts at: 2463 2734 2762 3056 5126 5622 2463 Size: 271 28 294 2070 496 2764 Fragments arranged by size: 2764 2070 496 294 271 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2381 1163 Size: 573 315 330 4705 Fragments arranged by size: 4705 573 330 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2480 3373 4134 5162 1540 Size: 275 186 479 893 761 1028 2301 Fragments arranged by size: 2301 1028 893 761 479 275 186 BsiHKAI G_wGCw'C Cuts at: 2420 2567 2796 3984 4069 5140 2420 Size: 147 229 1188 85 1071 3203 Fragments arranged by size: 3203 1188 1071 229 147 85 BsmI GAATG_Cn' Cuts at: 2026 2035 2131 2875 3434 3527 2026 Size: 9 96 744 559 93 4422 Fragments arranged by size: 4422 744 559 96 93 9 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2420 2464 2532 2567 2741 2761 Size: 399 360 270 44 68 35 174 20 Cuts at: 2761 2796 3242 3984 4069 5140 1391 Size: 35 446 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 360 270 174 85 68 44 35 35 20 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2270 3278 844 Size: 1426 1008 3489 Fragments arranged by size: 3489 1426 1008 BsrBI CCG'CTC Cuts at: 1394 1823 2241 2923 3810 1394 Size: 429 418 682 887 3507 Fragments arranged by size: 3507 887 682 429 418 BsrDI GCAATG_nn' Cuts at: 65 1248 2910 4418 4592 65 Size: 1183 1662 1508 174 1396 Fragments arranged by size: 1662 1508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2684 4567 4754 1700 Size: 110 204 670 1883 187 2869 Fragments arranged by size: 2869 1883 670 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 2795 2819 3983 4995 2795 Size: 24 1164 1012 3723 Fragments arranged by size: 3723 1164 1012 24 BstAPI GCAn_nnn'nTGC Cuts at: 794 2452 794 Size: 1658 4265 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 2702 1184 Size: 1518 4405 BstXI CCAn_nnnn'nTGG Cuts at: 2899 2899 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2331 3214 3253 3620 4005 4022 5474 5560 Size: 359 883 39 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 883 385 367 359 346 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3039 3039 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2614 513 Size: 552 1549 3822 Fragments arranged by size: 3822 1549 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3082 487 Size: 683 56 1856 3328 Fragments arranged by size: 3328 1856 683 56 BtsI GCAGTG_nn' Cuts at: 2126 2644 3439 4233 4253 2126 Size: 518 795 794 20 3796 Fragments arranged by size: 3796 795 794 518 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2222 4930 809 Size: 507 906 2708 1802 Fragments arranged by size: 2708 1802 906 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2297 2477 3182 Size: 54 1425 50 275 18 467 180 705 Cuts at: 3182 3370 4259 5888 8 Size: 188 889 1629 43 Fragments arranged by size: 1629 1425 889 705 467 275 188 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2477 3370 1537 Size: 275 665 893 4090 Fragments arranged by size: 4090 893 665 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2372 2563 4510 4884 5030 387 Size: 1064 138 783 191 1947 374 146 1280 Fragments arranged by size: 1947 1280 1064 783 374 191 146 138 EcoICRI GAG'CTC Cuts at: 2418 2565 2794 2418 Size: 147 229 5547 Fragments arranged by size: 5547 229 147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2404 2982 3083 3126 3167 3209 Size: 393 557 215 578 101 43 41 42 Cuts at: 3209 3986 4583 5370 5638 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 597 578 557 393 268 215 101 43 42 41 EcoO109I rG'GnC_Cy Cuts at: 2460 2594 2738 2757 2758 2957 2987 2460 Size: 134 144 19 1 199 30 5396 Fragments arranged by size: 5396 199 144 134 30 19 1 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 2909 4429 2909 Size: 1520 4403 HaeII r_GCGC'y Cuts at: 1678 2973 5070 1678 Size: 1295 2097 2531 Fragments arranged by size: 2531 2097 1295 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2364 2396 2549 2581 2873 Size: 32 135 32 792 32 153 32 292 Cuts at: 2873 2905 4564 4596 4638 4670 1373 Size: 32 1659 32 42 32 2626 Fragments arranged by size: 2626 1659 792 292 153 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2969 2969 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 3881 4240 4399 518 Size: 648 1341 1374 359 159 2042 Fragments arranged by size: 2042 1374 1341 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3339 4016 5164 5409 1606 Size: 1733 677 1148 245 2120 Fragments arranged by size: 2120 1733 1148 677 245 NaeI GCC'GGC Cuts at: 2016 2016 Size: 5923 NarI GG'CG_CC Cuts at: 2970 2970 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2014 2014 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2167 2446 3281 4826 1132 Size: 97 938 279 835 1545 2229 Fragments arranged by size: 2229 1545 938 835 279 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2783 2815 3992 4024 5505 5537 2783 Size: 32 1177 32 1481 32 3169 Fragments arranged by size: 3169 1481 1177 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2987 2987 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2460 2737 2757 2460 Size: 277 20 5626 Fragments arranged by size: 5626 277 20 PspXI vC'TCGA_Gb Cuts at: 2918 2918 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SacI G_AGCT'C Cuts at: 2420 2567 2796 2420 Size: 147 229 5547 Fragments arranged by size: 5547 229 147 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 2897 4171 5883 2897 Size: 1274 1712 2937 Fragments arranged by size: 2937 1712 1274 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2971 2971 Size: 5923 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 2014 1700 Size: 314 5609 SmaI CCC'GGG Cuts at: 1872 2533 3250 1872 Size: 661 717 4545 Fragments arranged by size: 4545 717 661 SmlI C'TyrA_G Cuts at: 820 1017 1943 2918 4027 4928 5190 820 Size: 197 926 975 1109 901 262 1553 Fragments arranged by size: 1553 1109 975 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 2446 3281 1132 Size: 1314 835 3774 Fragments arranged by size: 3774 1314 835 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2187 3210 3362 513 Size: 552 1122 1023 152 3074 Fragments arranged by size: 3074 1122 1023 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 2385 2663 2985 3950 3967 4120 Size: 1255 532 273 278 322 965 17 153 Cuts at: 4120 4305 4750 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 965 532 445 322 278 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 3084 4169 Size: 274 80 33 51 1443 918 189 1085 Cuts at: 4169 5881 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1085 918 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2544 2576 3199 3231 5883 5915 2544 Size: 32 623 32 2652 32 2552 Fragments arranged by size: 2652 2552 623 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 2918 1943 Size: 975 4948 XmaI C'CCGG_G Cuts at: 1870 2531 3248 1870 Size: 661 717 4545 Fragments arranged by size: 4545 717 661 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI