Restriction map for
pFN21AB8446
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FokI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MaeIII
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflII 148 CTTAAG C^TTAAG
AlwI 645 GATCC GGATC(4/5)
AlwNI 391 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 586 GGGCCC GGGCC^C
AspHI 803 GAGCTC G[AT]GC[AT]^C
BalI 830 TGGCCA TGG^CCA
BcnI 167 CCCGG CC^[GC]GG
BfrI 148 CTTAAG C^TTAAG
BglII 156 AGATCT A^GATCT
BsaI 710 GAGACC GGTCTC(1/5)
BsaWI 38 ACCGGA [AT]^CCGG[AT]
BsiHKAI 803 GAGCTC G[AT]GC[AT]^C
BsmI 206 GAATGC GAATGC(1/-1)
BsrBI 766 GAGCGG CCGCTC(-3/-3)
BsrFI 725 ACCGGC [GA]^CCGG[CT]
BsrI 719 ACTGG ACTGG(1/-1)
BstXI 575 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 725 ACCGGC [GA]^CCGG[CT]
DraI 231 TTTAAA TTT^AAA
DraIII 592 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 887 CCACGG C^C[GA][CT]GG
EaeI 830 TGGCCA [CT]^GGCC[GA]
EcoNI 828 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 198 GATATC GAT^ATC
FauI 561 CCCGC CCCGC(4/6)
HaeII 701 AGCGCC [GA]GCGC^[CT]
HphI 524 TCACC GGTGA(8/7)
MluNI 830 TGGCCA TGG^CCA
MscI 830 TGGCCA TGG^CCA
NciI 167 CCCGG CC^[GC]GG
PleI 463 GAGTC GAGTC(4/5)
PpuMI 542 GGGACCT [GA]G^G[AT]CC[CT]
PstI 361 CTGCAG CTGCA^G
PvuII 634 CAGCTG CAG^CTG
SacI 803 GAGCTC GAGCT^C
SfaNI 427 GCATC GCATC(5/9)
SmlI 148 CTTAAG C^T[CT][GA]AG
TfiI 735 GATTC G^A[AT]TC
XcmI 458 CCATCGAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 249 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
435 GAAAGACTTC
AviII 331 TGCGCA TGC^GCA
797 TGCGCA
BbvCI 822 CCTCAGC CCTCAGC(-5/-2)
681 GCTGAGG
BfaI 142 CTAG C^TAG
652 CTAG
BspMI 545 ACCTGC ACCTGC(4/8)
794 ACCTGC
BstAPI 418 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
840 GCAGCATTTGC
BstYI 156 AGATCT [GA]^GATC[CT]
644 AGATCC
EcoT14I 581 CCTTGG C^C[AT][AT]GG
833 CCAAGG
FspI 331 TGCGCA TGC^GCA
797 TGCGCA
HinfI 463 GAGTC G^A[ACGT]TC
735 GATTC
MaeI 142 CTAG C^TAG
652 CTAG
MflI 156 AGATCT [GA]^GATC[CT]
644 AGATCC
MspA1I 609 CCGCTG C[AC]G^C[GT]G
634 CAGCTG
PflMI 390 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCACCGCGTGG
SfcI 66 CTGTAG C^T[GA][CT]AG
361 CTGCAG
StyI 581 CCTTGG C^C[AT][AT]GG
833 CCAAGG
TaqI 461 TCGA T^CGA
924 TCGA
TspRI 467 CAGTG CA[GC]TG[ACGT][ACGT]^
775 CAGTG
TthHB8I 461 TCGA T^CGA
924 TCGA
Van91I 390 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCACCGCGTGG
XhoII 156 AGATCT [GA]^GATC[CT]
644 AGATCC
XmnI 249 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
435 GAAAGACTTC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AvaII BanII BbvI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MseI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I