(Circular) (Six-base) MAPSORT of: pFN21AB8913.seq Check: 4319 from: 1 to: 5464 pFN21AB8913 5464 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2598 2807 1937 Size: 661 209 4594 Fragments arranged by size: 4594 661 209 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 3527 4911 5179 1632 Size: 1058 837 1384 268 1917 Fragments arranged by size: 1917 1384 1058 837 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AlwNI CAG_nnn'CTG Cuts at: 2366 3207 4779 2366 Size: 841 1572 3051 Fragments arranged by size: 3051 1572 841 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2259 2789 1870 Size: 73 136 180 530 4545 Fragments arranged by size: 4545 530 180 136 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2208 2785 4240 618 Size: 325 217 627 342 79 577 1455 1842 Fragments arranged by size: 1842 1455 627 577 342 325 217 79 BanII G_rGCy'C Cuts at: 1391 2049 2783 1391 Size: 658 734 4072 Fragments arranged by size: 4072 734 658 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2478 928 Size: 971 579 3914 Fragments arranged by size: 3914 971 579 BbvCI CC'TCA_GC Cuts at: 2061 2061 Size: 5464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 2178 1548 Size: 630 4834 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2057 4075 Size: 107 122 71 631 605 337 48 2018 Cuts at: 4075 136 Size: 1525 Fragments arranged by size: 2018 1525 631 605 337 122 107 71 48 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 3525 4681 1790 Size: 1735 1156 2573 Fragments arranged by size: 2573 1735 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2294 3195 4153 Size: 416 53 83 399 24 877 901 958 Cuts at: 4153 442 Size: 1753 Fragments arranged by size: 1753 958 901 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2618 2618 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2240 2633 4124 1239 Size: 893 108 393 1491 2579 Fragments arranged by size: 2579 1491 893 393 108 Bpu10I CC'TnA_GC Cuts at: 2061 2061 Size: 5464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2122 3589 4454 4752 2122 Size: 1467 865 298 2834 Fragments arranged by size: 2834 1467 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2423 4127 882 Size: 617 924 1704 2219 Fragments arranged by size: 2219 1704 924 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2669 2699 1178 Size: 30 165 30 1266 30 3943 Fragments arranged by size: 3943 1266 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2258 2677 1186 Size: 871 201 419 3973 Fragments arranged by size: 3973 871 419 201 BseYI C'CCAG_C Cuts at: 4667 5163 4667 Size: 496 4968 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 1163 Size: 573 534 4357 Fragments arranged by size: 4357 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2914 3675 4703 1540 Size: 275 186 33 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2547 3525 3610 4681 2547 Size: 978 85 1071 3330 Fragments arranged by size: 3330 1071 978 85 BsmI GAATG_Cn' Cuts at: 2466 2975 3068 2466 Size: 509 93 4862 Fragments arranged by size: 4862 509 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2547 2783 3525 3610 4681 1391 Size: 399 259 498 236 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 259 236 85 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2437 2819 844 Size: 1593 382 3489 Fragments arranged by size: 3489 1593 382 BsrBI CCG'CTC Cuts at: 1394 1823 2086 2106 3351 1394 Size: 429 263 20 1245 3507 Fragments arranged by size: 3507 1245 429 263 20 BsrDI GCAATG_nn' Cuts at: 65 1248 3959 4133 65 Size: 1183 2711 174 1396 Fragments arranged by size: 2711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2205 4108 4295 1700 Size: 110 219 176 1903 187 2869 Fragments arranged by size: 2869 1903 219 187 176 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 2138 1925 Size: 213 5251 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459 Size: 822 367 385 17 1452 86 12 346 Cuts at: 5459 1972 Size: 1977 Fragments arranged by size: 1977 1452 822 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2149 2754 513 Size: 552 939 145 605 3223 Fragments arranged by size: 3223 939 605 552 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2980 3774 3794 2980 Size: 794 20 4650 Fragments arranged by size: 4650 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2606 4471 809 Size: 507 1290 1865 1802 Fragments arranged by size: 1865 1802 1290 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2031 2070 Size: 54 1425 50 275 18 183 18 39 Cuts at: 2070 2152 2911 3800 5429 8 Size: 82 759 889 1629 43 Fragments arranged by size: 1629 1425 889 759 275 183 82 54 50 43 39 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 2911 1537 Size: 275 219 880 4090 Fragments arranged by size: 4090 880 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 3394 1353 Size: 1313 728 3423 Fragments arranged by size: 3423 1313 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 4051 4425 4571 387 Size: 1064 138 668 1794 374 146 1280 Fragments arranged by size: 1794 1280 1064 668 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2240 2633 2690 3527 4124 4911 Size: 393 500 108 393 57 837 597 787 Cuts at: 4911 5179 1239 Size: 268 1524 Fragments arranged by size: 1524 837 787 597 500 393 393 268 108 57 EcoNI CCTnn'n_nnAGG Cuts at: 2615 2615 Size: 5464 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 5464 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2219 2267 4611 1678 Size: 541 48 2344 2531 Fragments arranged by size: 2531 2344 541 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373 Size: 32 135 32 2533 32 42 32 2626 Fragments arranged by size: 2626 2533 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 2439 3063 2439 Size: 624 4840 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2352 2448 2526 4578 4762 1305 Size: 1047 96 78 2052 184 2007 Fragments arranged by size: 2052 2007 1047 184 96 78 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3642 Fragments arranged by size: 3642 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3422 3781 3940 518 Size: 648 2256 359 159 2042 Fragments arranged by size: 2256 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2274 2533 2880 3557 4705 4950 1606 Size: 668 259 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 347 259 245 NaeI GCC'GGC Cuts at: 2031 2031 Size: 5464 NcoI C'CATG_G Cuts at: 513 1065 2004 2754 513 Size: 552 939 750 3223 Fragments arranged by size: 3223 939 750 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NgoMIV G'CCGG_C Cuts at: 2029 2029 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2509 2822 4367 1132 Size: 97 1280 313 1545 2229 Fragments arranged by size: 2229 1545 1280 313 97 PasI CC'CwG_GG Cuts at: 1348 2413 1348 Size: 1065 4399 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PfoI T'CCnGG_A Cuts at: 2610 2610 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PstI C_TGCA'G Cuts at: 830 2816 830 Size: 1986 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 PvuII CAG'CTG Cuts at: 2533 2533 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SapI GCTCTTCn'nnn_ Cuts at: 2666 2666 Size: 5464 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2812 3947 4628 4819 826 Size: 219 1767 1135 681 191 1471 Fragments arranged by size: 1767 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2081 2261 2791 1872 Size: 209 180 530 4545 Fragments arranged by size: 4545 530 209 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 2101 3568 4469 4731 820 Size: 197 926 158 1467 901 262 1553 Fragments arranged by size: 1553 1467 926 901 262 197 158 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SrfI GCCC'GGGC Cuts at: 2261 2261 Size: 5464 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 2621 1517 Size: 1104 4360 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 2754 2903 513 Size: 552 939 192 558 149 3074 Fragments arranged by size: 3074 939 558 552 192 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3491 3508 3661 3846 4291 325 Size: 1255 1911 17 153 185 445 1498 Fragments arranged by size: 1911 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3710 5422 96 Size: 274 80 33 51 1443 1733 1712 138 Fragments arranged by size: 1733 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2079 2259 2789 1870 Size: 209 180 530 4545 Fragments arranged by size: 4545 530 209 180 XmnI GAAnn'nnTTC Cuts at: 2621 2664 3593 2621 Size: 43 929 4492 Fragments arranged by size: 4492 929 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI