(Circular) (Six-base) MAPSORT of: pFN21AB7855.seq Check: 5241 from: 1 to: 6358 pFN21AB7855 6358 bp With 182 enzymes: SgfI * May 13, 2009 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3283 3283 Size: 6358 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4168 278 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 3701 1937 Size: 1474 290 4594 Fragments arranged by size: 4594 1474 290 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 4485 4858 1563 Size: 2922 373 3063 Fragments arranged by size: 3063 2922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4421 5805 6073 1632 Size: 2789 1384 268 1917 Fragments arranged by size: 2789 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6161 AflIII A'CryG_T Cuts at: 1203 5257 6234 1203 Size: 4054 977 1327 Fragments arranged by size: 4054 1327 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 3101 1166 Size: 1935 4423 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3017 1184 Size: 1833 4525 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 6326 AlwNI CAG_nnn'CTG Cuts at: 2350 2869 3023 3182 3230 3433 3436 3548 4101 Size: 519 154 159 48 203 3 112 553 Cuts at: 4101 5673 2350 Size: 1572 3035 Fragments arranged by size: 3035 1572 553 519 203 159 154 112 48 3 ApaI G_GGCC'C Cuts at: 3526 3565 3526 Size: 39 6319 ApaLI G'TGCA_C Cuts at: 2735 4415 5571 2735 Size: 1680 1156 3522 Fragments arranged by size: 3522 1680 1156 ApoI r'AATT_y Cuts at: 1493 2858 3667 3893 4045 6154 1493 Size: 1365 809 226 152 2109 1697 Fragments arranged by size: 2109 1697 1365 809 226 152 AseI AT'TA_AT Cuts at: 160 1032 4912 160 Size: 872 3880 1606 Fragments arranged by size: 3880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 2709 3565 3683 1870 Size: 73 766 856 118 4545 Fragments arranged by size: 4545 856 766 118 73 BamHI G'GATC_C Cuts at: 3688 4055 3688 Size: 367 5991 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3679 5134 618 Size: 325 217 627 1892 1455 1842 Fragments arranged by size: 1892 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2316 2586 2844 2853 3477 3526 3565 3573 Size: 925 270 258 9 624 49 39 8 Cuts at: 3573 3677 1391 Size: 104 4072 Fragments arranged by size: 4072 925 624 270 258 104 49 39 9 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3354 3606 928 Size: 971 1455 252 3680 Fragments arranged by size: 3680 1455 971 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2450 2484 2605 2639 4549 4583 1903 Size: 34 513 34 121 34 1910 34 3678 Fragments arranged by size: 3678 1910 513 121 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 4969 136 Size: 107 122 71 631 605 623 2674 1525 Fragments arranged by size: 2674 1525 631 623 605 122 107 71 BglII A'GATC_T Cuts at: 2589 2637 6353 2589 Size: 48 3716 2594 Fragments arranged by size: 3716 2594 48 BlpI GC'TnA_GC Cuts at: 2310 3016 3775 2310 Size: 706 759 4893 Fragments arranged by size: 4893 759 706 Bme1580I G_kGCm'C Cuts at: 1790 2569 2739 3526 3565 4419 5575 1790 Size: 779 170 787 39 854 1156 2573 Fragments arranged by size: 2573 1156 854 787 779 170 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3230 3566 4089 Size: 416 53 83 399 24 1813 336 523 Cuts at: 4089 5047 442 Size: 958 1753 Fragments arranged by size: 1813 1753 958 523 416 399 336 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 3159 3177 5018 1239 Size: 1163 757 18 1841 2579 Fragments arranged by size: 2579 1841 1163 757 18 Bpu10I CC'TnA_GC Cuts at: 3068 3068 Size: 6358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 4483 5348 5646 2570 Size: 1913 865 298 3282 Fragments arranged by size: 3282 1913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2918 5021 882 Size: 617 1419 2103 2219 Fragments arranged by size: 2219 2103 1419 617 BsaAI yAC'GTr Cuts at: 493 1204 4149 493 Size: 711 2945 2702 Fragments arranged by size: 2945 2702 711 BsaBI GATnn'nnATC Cuts at: 6352 6352 Size: 6358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4165 4547 5182 275 Size: 53 83 186 936 2632 382 635 1451 Fragments arranged by size: 2632 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2760 2892 4791 5189 5463 5610 6021 2760 Size: 132 1899 398 274 147 411 3097 Fragments arranged by size: 3097 1899 411 398 274 147 132 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3593 3623 1178 Size: 30 165 30 2190 30 3913 Fragments arranged by size: 3913 2190 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2167 2238 2915 3246 3598 1186 Size: 981 71 677 331 352 3946 Fragments arranged by size: 3946 981 677 352 331 71 BseYI C'CCAG_C Cuts at: 2700 2783 3042 3223 3296 3382 3541 5561 6057 Size: 83 259 181 73 86 159 2020 496 Cuts at: 6057 2700 Size: 3001 Fragments arranged by size: 3001 2020 496 259 181 159 86 83 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2681 1163 Size: 573 945 4840 Fragments arranged by size: 4840 945 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2836 2887 3808 4569 5597 1540 Size: 275 186 835 51 921 761 1028 2301 Fragments arranged by size: 2301 1028 921 835 761 275 186 51 BsiHKAI G_wGCw'C Cuts at: 2159 2316 2586 2739 2844 3074 4419 4504 5575 Size: 157 270 153 105 230 1345 85 1071 Cuts at: 5575 2159 Size: 2942 Fragments arranged by size: 2942 1345 1071 270 230 157 153 105 85 BsmI GAATG_Cn' Cuts at: 2473 3869 3962 2473 Size: 1396 93 4869 Fragments arranged by size: 4869 1396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2316 2569 2586 2739 2844 2853 Size: 399 369 157 253 17 153 105 9 Cuts at: 2853 3074 3477 3526 3565 3573 3677 4419 4504 Size: 221 403 49 39 8 104 742 85 Cuts at: 4504 5575 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 369 253 221 157 153 105 104 85 49 39 17 9 8 BspEI T'CCGG_A Cuts at: 2760 6021 2760 Size: 3261 3097 BspHI T'CATG_A Cuts at: 2940 4247 5977 2940 Size: 1307 1730 3321 Fragments arranged by size: 3321 1730 1307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2877 3283 3713 844 Size: 2033 406 430 3489 Fragments arranged by size: 3489 2033 430 406 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2994 3612 4853 5027 65 Size: 1183 1746 618 1241 174 1396 Fragments arranged by size: 1746 1396 1241 1183 618 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2253 5002 5189 1700 Size: 110 443 2749 187 2869 Fragments arranged by size: 2869 2749 443 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 2267 4418 5430 2267 Size: 2151 1012 3195 Fragments arranged by size: 3195 2151 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6358 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 2233 1184 Size: 1049 5309 BstXI CCAn_nnnn'nTGG Cuts at: 2054 3484 2054 Size: 1430 4928 BstYI r'GATC_y Cuts at: 1972 2589 2637 3688 4055 4440 4457 5909 5995 Size: 617 48 1051 367 385 17 1452 86 Cuts at: 5995 6007 6353 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1051 617 385 367 346 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3258 3495 3258 Size: 237 6121 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 2853 3099 3198 513 Size: 552 939 126 723 246 99 3673 Fragments arranged by size: 3673 939 723 552 246 126 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5619 Fragments arranged by size: 5619 683 56 BtsI GCAGTG_nn' Cuts at: 2574 3874 4668 4688 2574 Size: 1300 794 20 4244 Fragments arranged by size: 4244 1300 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 3662 3662 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2748 3805 4694 Size: 54 1425 50 275 18 918 1057 889 Cuts at: 4694 6323 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1057 918 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 2942 4288 1353 Size: 1083 506 1346 3423 Fragments arranged by size: 3423 1346 1083 506 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2513 4945 5319 5465 387 Size: 1064 138 924 2432 374 146 1280 Fragments arranged by size: 2432 1280 1064 924 374 146 138 EcoICRI GAG'CTC Cuts at: 2314 2584 2842 2314 Size: 270 258 5830 Fragments arranged by size: 5830 270 258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 3159 3177 4421 5018 5805 6073 Size: 393 770 757 18 1244 597 787 268 Cuts at: 6073 1239 Size: 1524 Fragments arranged by size: 1524 1244 787 770 757 597 393 268 18 EcoNI CCTnn'n_nnAGG Cuts at: 2384 3167 2384 Size: 783 5575 EcoO109I rG'GnC_Cy Cuts at: 2242 2377 2503 2517 3220 3251 3319 3357 3522 Size: 135 126 14 703 31 68 38 165 Cuts at: 3522 3531 3561 3562 2242 Size: 9 30 1 5038 Fragments arranged by size: 5038 703 165 135 126 68 38 31 30 14 9 1 EcoRI G'AATT_C Cuts at: 2858 3667 4045 2858 Size: 809 378 5171 Fragments arranged by size: 5171 809 378 FspI TGC'GCA Cuts at: 2222 4864 2222 Size: 2642 3716 FspAI rTGC'GCAy Cuts at: 2222 2222 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 2128 5505 1678 Size: 450 3377 2531 Fragments arranged by size: 3377 2531 450 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3389 3421 3593 3625 4999 Size: 32 135 32 1817 32 172 32 1374 Cuts at: 4999 5031 5073 5105 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1817 1374 172 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3412 3702 3948 669 Size: 988 281 1474 290 246 3079 Fragments arranged by size: 3079 1474 988 290 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6358 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2856 5472 5656 1305 Size: 1551 2616 184 2007 Fragments arranged by size: 2616 2007 1551 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 10 Size: 54 1425 343 918 3618 Fragments arranged by size: 3618 1425 918 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 2999 3101 3452 3482 4316 4675 Size: 648 1512 321 102 351 30 834 359 Cuts at: 4675 4834 518 Size: 159 2042 Fragments arranged by size: 2042 1512 834 648 359 351 321 159 102 30 MspA1I CmG'CkG Cuts at: 1606 2092 2132 2328 2619 2818 2961 3331 3386 Size: 486 40 196 291 199 143 370 55 Cuts at: 3386 3433 3774 4451 5599 5844 1606 Size: 47 341 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 486 370 341 291 245 199 196 143 55 47 40 NcoI C'CATG_G Cuts at: 513 1065 2004 2853 3099 3198 513 Size: 552 939 849 246 99 3673 Fragments arranged by size: 3673 939 849 552 246 99 NdeI CA'TA_TG Cuts at: 387 2920 387 Size: 2533 3825 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 2101 3716 5261 1132 Size: 97 872 1615 1545 2229 Fragments arranged by size: 2229 1615 1545 872 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 5257 5257 Size: 6358 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 1108 Size: 1242 5116 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2594 2626 4427 4459 5940 5972 2594 Size: 32 1801 32 1481 32 2980 Fragments arranged by size: 2980 1801 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2377 2503 2242 Size: 135 126 6097 Fragments arranged by size: 6097 135 126 PshAI GACnn'nnGTC Cuts at: 1613 2886 1613 Size: 1273 5085 PsiI TTA'TAA Cuts at: 3928 3928 Size: 6358 PspOMI G'GGCC_C Cuts at: 3522 3561 3522 Size: 39 6319 PstI C_TGCA'G Cuts at: 830 2793 3330 3336 3710 830 Size: 1963 537 6 374 3478 Fragments arranged by size: 3478 1963 537 374 6 PvuI CG_AT'CG Cuts at: 2001 2887 2001 Size: 886 5472 PvuII CAG'CTG Cuts at: 2619 2818 3331 3386 3433 2619 Size: 199 513 55 47 5544 Fragments arranged by size: 5544 513 199 55 47 RsrII CG'GwC_CG Cuts at: 2323 2323 Size: 6358 SacI G_AGCT'C Cuts at: 2316 2586 2844 2316 Size: 270 258 5830 Fragments arranged by size: 5830 270 258 SacII CC_GC'GG Cuts at: 2133 2133 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3410 3700 1936 Size: 1474 290 4594 Fragments arranged by size: 4594 1474 290 SanDI GG'GwC_CC Cuts at: 2503 2503 Size: 6358 SapI GCTCTTCn'nnn_ Cuts at: 2436 2436 Size: 6358 SbfI CC_TGCA'GG Cuts at: 2793 3710 2793 Size: 917 5441 ScaI AGT'ACT Cuts at: 4606 6318 4606 Size: 1712 4646 SfcI C'TryA_G Cuts at: 826 1045 2436 2789 3326 3332 3706 4841 5522 Size: 219 1391 353 537 6 374 1135 681 Cuts at: 5522 5713 826 Size: 191 1471 Fragments arranged by size: 1471 1391 1135 681 537 374 353 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3567 3685 1872 Size: 1695 118 4545 Fragments arranged by size: 4545 1695 118 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 4462 5363 5625 820 Size: 197 926 642 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 642 262 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 2101 3716 1132 Size: 969 1615 3774 Fragments arranged by size: 3774 1615 969 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 2051 3131 1517 Size: 534 1080 4744 Fragments arranged by size: 4744 1080 534 StyI C'CwwG_G Cuts at: 513 1065 2004 2238 2853 3099 3138 3198 3797 Size: 552 939 234 615 246 39 60 599 Cuts at: 3797 513 Size: 3074 Fragments arranged by size: 3074 939 615 599 552 246 234 60 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3305 4385 4402 4555 4740 5185 325 Size: 1255 1725 1080 17 153 185 445 1498 Fragments arranged by size: 1725 1498 1255 1080 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4604 6316 96 Size: 274 80 33 51 1443 2627 1712 138 Fragments arranged by size: 2627 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6318 6350 6318 Size: 32 6326 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3619 3619 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3565 3683 1870 Size: 1695 118 4545 Fragments arranged by size: 4545 1695 118 XmnI GAAnn'nnTTC Cuts at: 4487 4487 Size: 6358 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4166 276 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SexAI SfiI SfoI SrfI SwaI