(Circular) (Six-base) MAPSORT of: pFN21AB5186.seq Check: 9985 from: 1 to: 5548 pFN21AB5186 5548 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 2891 1937 Size: 280 674 4594 Fragments arranged by size: 4594 674 280 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 4995 5263 1632 Size: 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 268 AfeI AGC'GCT Cuts at: 2513 2834 2513 Size: 321 5227 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 2238 4447 5424 1203 Size: 1035 2209 977 1327 Fragments arranged by size: 2209 1327 1035 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 2377 4277 2377 Size: 1900 3648 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 2549 Size: 32 5516 AlwNI CAG_nnn'CTG Cuts at: 3291 4863 3291 Size: 1572 3976 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2857 3083 3235 5344 1493 Size: 1364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2719 2873 1870 Size: 73 95 681 154 4545 Fragments arranged by size: 4545 681 154 95 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2271 2743 2869 4324 618 Size: 325 217 627 484 472 126 1455 1842 Fragments arranged by size: 1842 1455 627 484 472 325 217 126 BanII G_rGCy'C Cuts at: 1391 2612 2867 1391 Size: 1221 255 4072 Fragments arranged by size: 4072 1221 255 BbeI G_GCGC'C Cuts at: 2275 2275 Size: 5548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2069 3440 4650 2069 Size: 1371 1210 2967 Fragments arranged by size: 2967 1371 1210 BclI T'GATC_A Cuts at: 1548 2022 1548 Size: 474 5074 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4159 136 Size: 107 122 71 631 605 2487 1525 Fragments arranged by size: 2487 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2529 5543 2529 Size: 3014 2534 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 2039 2421 3609 4765 1790 Size: 249 382 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 382 249 BmgBI CAC'GTC Cuts at: 1318 2241 2416 2650 1318 Size: 923 175 234 4216 Fragments arranged by size: 4216 923 234 175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4208 1239 Size: 2969 2579 Bpu10I CC'TnA_GC Cuts at: 2354 2354 Size: 5548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2372 3673 4538 4836 2372 Size: 1301 865 298 3084 Fragments arranged by size: 3084 1301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2635 4211 882 Size: 617 1136 1576 2219 Fragments arranged by size: 2219 1576 1136 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 2272 2727 3355 Size: 53 83 186 936 624 115 455 628 Cuts at: 3355 3737 4372 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 628 624 455 382 186 115 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 1178 Size: 30 165 30 912 30 4381 Fragments arranged by size: 4381 912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 1186 Size: 1168 4380 BseYI C'CCAG_C Cuts at: 2508 2597 2762 4751 5247 2508 Size: 89 165 1989 496 2809 Fragments arranged by size: 2809 1989 496 165 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2603 2639 2657 1163 Size: 573 630 237 36 18 4054 Fragments arranged by size: 4054 630 573 237 36 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2146 2998 3759 4787 1540 Size: 275 186 145 852 761 1028 2301 Fragments arranged by size: 2301 1028 852 761 275 186 145 BsiHKAI G_wGCw'C Cuts at: 3609 3694 4765 3609 Size: 85 1071 4392 Fragments arranged by size: 4392 1071 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 BsmBI CGTCTCn'nnnn_ Cuts at: 2286 2286 Size: 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2421 2612 2867 3609 3694 4765 Size: 399 249 382 191 255 742 85 1071 Cuts at: 4765 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 382 255 249 191 85 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 2903 844 Size: 1781 278 3489 Fragments arranged by size: 3489 1781 278 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 2693 2757 2807 4192 4379 1700 Size: 110 331 552 64 50 1385 187 2869 Fragments arranged by size: 2869 1385 552 331 187 110 64 50 BsrGI T'GTAC_A Cuts at: 96 2784 96 Size: 2688 2860 BssHII G'CGCG_C Cuts at: 1925 2571 2753 1925 Size: 646 182 4720 Fragments arranged by size: 4720 646 182 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 2444 1184 Size: 1260 4288 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2364 2529 2878 3245 3630 3647 5099 5185 Size: 392 165 349 367 385 17 1452 86 Cuts at: 5185 5197 5543 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 392 385 367 349 346 165 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2206 2583 513 Size: 552 1141 377 3478 Fragments arranged by size: 3478 1141 552 377 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2033 487 Size: 683 56 807 4002 Fragments arranged by size: 4002 807 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3858 3878 3064 Size: 794 20 4734 Fragments arranged by size: 4734 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2064 2118 Size: 54 1425 50 275 18 183 51 54 Cuts at: 2118 2143 2535 2586 2995 3884 5513 8 Size: 25 392 51 409 889 1629 43 Fragments arranged by size: 1629 1425 889 409 392 275 183 54 54 51 51 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2143 2995 1537 Size: 275 331 852 4090 Fragments arranged by size: 4090 852 331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2582 3478 1353 Size: 1229 896 3423 Fragments arranged by size: 3423 1229 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3611 4208 4995 5263 1239 Size: 393 1979 597 787 268 1524 Fragments arranged by size: 1979 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 2275 2515 2669 2808 2836 4695 1678 Size: 597 240 154 139 28 1859 2531 Fragments arranged by size: 2531 1859 597 240 154 139 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 4189 4221 4263 Size: 32 135 32 791 32 1794 32 42 Cuts at: 4263 4295 1373 Size: 32 2626 Fragments arranged by size: 2626 1794 791 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KasI G'GCGC_C Cuts at: 2271 2271 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2588 10 Size: 54 1425 343 756 2970 Fragments arranged by size: 2970 1425 756 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 3506 3865 4024 518 Size: 648 1140 1200 359 159 2042 Fragments arranged by size: 2042 1200 1140 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2208 2350 2491 2700 2964 3641 4789 5034 Size: 602 142 141 209 264 677 1148 245 Cuts at: 5034 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 602 264 245 209 142 141 NaeI GCC'GGC Cuts at: 2695 2759 2809 2695 Size: 64 50 5434 Fragments arranged by size: 5434 64 50 NarI GG'CG_CC Cuts at: 2272 2272 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 2583 513 Size: 552 1518 3478 Fragments arranged by size: 3478 1518 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NgoMIV G'CCGG_C Cuts at: 2693 2757 2807 2693 Size: 64 50 5434 Fragments arranged by size: 5434 64 50 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PstI C_TGCA'G Cuts at: 830 2900 830 Size: 2070 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2491 2491 Size: 5548 SacII CC_GC'GG Cuts at: 2209 2209 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SapI GCTCTTCn'nnn_ Cuts at: 2582 2582 Size: 5548 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 2642 3796 5508 2642 Size: 1154 1712 2682 Fragments arranged by size: 2682 1712 1154 SfcI C'TryA_G Cuts at: 826 1045 2896 4031 4712 4903 826 Size: 219 1851 1135 681 191 1471 Fragments arranged by size: 1851 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2273 2273 Size: 5548 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2721 2875 1872 Size: 849 154 4545 Fragments arranged by size: 4545 849 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 2387 3652 4553 4815 820 Size: 197 926 444 1265 901 262 1553 Fragments arranged by size: 1553 1265 926 901 444 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2103 2400 2583 2820 2987 513 Size: 552 1038 297 183 237 167 3074 Fragments arranged by size: 3074 1038 552 297 237 183 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3575 3592 3745 3930 4375 325 Size: 1255 1995 17 153 185 445 1498 Fragments arranged by size: 1995 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2640 2784 3794 Size: 274 80 33 51 1443 663 144 1010 Cuts at: 3794 5506 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1010 663 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2407 2407 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2719 2873 1870 Size: 849 154 4545 Fragments arranged by size: 4545 849 154 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI