(Circular) (Six-base) MAPSORT of: pFN21AE0159.seq Check: 8748 from: 1 to: 5437 pFN21AE0159 5437 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2584 3500 4884 5152 1632 Size: 952 916 1384 268 1917 Fragments arranged by size: 1917 1384 952 916 268 AfeI AGC'GCT Cuts at: 2152 2152 Size: 5437 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2272 1184 Size: 1088 4349 AlwNI CAG_nnn'CTG Cuts at: 2494 2607 3180 4752 2494 Size: 113 573 1572 3179 Fragments arranged by size: 3179 1572 573 113 ApaI G_GGCC'C Cuts at: 2101 2101 Size: 5437 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2621 2746 2972 3124 5233 1493 Size: 1128 125 226 152 2109 1697 Fragments arranged by size: 2109 1697 1128 226 152 125 AscI GG'CGCG_CC Cuts at: 2033 2033 Size: 5437 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2522 2762 1870 Size: 73 579 240 4545 Fragments arranged by size: 4545 579 240 73 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2758 4213 618 Size: 325 217 627 971 1455 1842 Fragments arranged by size: 1842 1455 971 627 325 217 BanII G_rGCy'C Cuts at: 1391 2101 2675 2756 1391 Size: 710 574 81 4072 Fragments arranged by size: 4072 710 574 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BbvCI CC'TCA_GC Cuts at: 2211 2211 Size: 5437 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2075 2109 3628 3662 1903 Size: 34 138 34 1519 34 3678 Fragments arranged by size: 3678 1519 138 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2554 3329 4539 2554 Size: 775 1210 3452 Fragments arranged by size: 3452 1210 775 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4048 136 Size: 107 122 71 631 605 337 2039 1525 Fragments arranged by size: 2039 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 2101 3498 4654 1790 Size: 311 1397 1156 2573 Fragments arranged by size: 2573 1397 1156 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4126 442 Size: 416 53 83 399 24 1751 958 1753 Fragments arranged by size: 1753 1751 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2157 1056 Size: 1101 4336 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 2341 4097 1239 Size: 1047 55 1756 2579 Fragments arranged by size: 2579 1756 1047 55 Bpu10I CC'TnA_GC Cuts at: 2211 2674 2211 Size: 463 4974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 3562 4427 4725 2366 Size: 1196 865 298 3078 Fragments arranged by size: 3078 1196 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3244 3626 4261 275 Size: 53 83 186 936 1711 382 635 1451 Fragments arranged by size: 1711 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2588 2618 1178 Size: 30 165 30 1185 30 3997 Fragments arranged by size: 3997 1185 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5437 BseYI C'CCAG_C Cuts at: 2498 2725 4640 5136 2498 Size: 227 1915 496 2799 Fragments arranged by size: 2799 1915 496 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 1163 Size: 573 369 4495 Fragments arranged by size: 4495 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2669 2887 3648 4676 1540 Size: 275 186 668 218 761 1028 2301 Fragments arranged by size: 2301 1028 761 668 275 218 186 BsiHKAI G_wGCw'C Cuts at: 2547 2675 3498 3583 4654 2547 Size: 128 823 85 1071 3330 Fragments arranged by size: 3330 1071 823 128 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2547 2675 2756 3498 3583 4654 Size: 399 311 446 128 81 742 85 1071 Cuts at: 4654 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 446 399 311 128 85 81 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2792 844 Size: 1948 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3324 1394 Size: 429 1501 3507 Fragments arranged by size: 3507 1501 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 3932 4106 65 Size: 1183 1042 1642 174 1396 Fragments arranged by size: 1642 1396 1183 1042 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4081 4268 1700 Size: 110 2271 187 2869 Fragments arranged by size: 2869 2271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 2033 1925 Size: 108 5329 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 2420 794 Size: 1626 3811 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 2551 2695 1184 Size: 1367 144 3926 Fragments arranged by size: 3926 1367 144 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432 Size: 795 367 385 17 1452 86 12 346 Cuts at: 5432 1972 Size: 1977 Fragments arranged by size: 1977 1452 795 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2589 2589 Size: 5437 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2521 487 Size: 683 56 1295 3403 Fragments arranged by size: 3403 1295 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2460 2884 3773 Size: 54 1425 50 275 18 630 424 889 Cuts at: 3773 5402 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 630 424 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 1537 Size: 275 1072 4090 Fragments arranged by size: 4090 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2229 3367 1353 Size: 783 93 1138 3423 Fragments arranged by size: 3423 1138 783 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2673 2673 Size: 5437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2341 2584 3500 4097 4884 5152 Size: 393 654 55 243 916 597 787 268 Cuts at: 5152 1239 Size: 1524 Fragments arranged by size: 1524 916 787 654 597 393 268 243 55 EcoNI CCTnn'n_nnAGG Cuts at: 2687 2687 Size: 5437 EcoRI G'AATT_C Cuts at: 2621 2746 3124 2621 Size: 125 378 4934 Fragments arranged by size: 4934 378 125 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2320 2320 Size: 5437 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 2067 2154 4584 1678 Size: 389 87 2430 2531 Fragments arranged by size: 2531 2430 389 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 4078 4110 4152 Size: 32 135 32 1016 32 1458 32 42 Cuts at: 4152 4184 1373 Size: 32 2626 Fragments arranged by size: 2626 1458 1016 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2197 4551 4735 1305 Size: 892 2354 184 2007 Fragments arranged by size: 2354 2007 892 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 10 Size: 54 1425 343 630 2985 Fragments arranged by size: 2985 1425 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 3395 3754 3913 518 Size: 648 1374 855 359 159 2042 Fragments arranged by size: 2042 1374 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2511 2853 3530 4678 4923 1606 Size: 905 342 677 1148 245 2120 Fragments arranged by size: 2120 1148 905 677 342 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 2339 387 Size: 1952 3485 NheI G'CTAG_C Cuts at: 1052 2153 1052 Size: 1101 4336 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2518 2795 4340 1132 Size: 97 1289 277 1545 2229 Fragments arranged by size: 2229 1545 1289 277 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 2494 1108 Size: 1386 4051 PfoI T'CCnGG_A Cuts at: 2606 2606 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspOMI G'GGCC_C Cuts at: 2097 2097 Size: 5437 PstI C_TGCA'G Cuts at: 830 2026 2789 830 Size: 1196 763 3478 Fragments arranged by size: 3478 1196 763 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SacI G_AGCT'C Cuts at: 2675 2675 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SexAI A'CCwGG_T Cuts at: 2199 2199 Size: 5437 SfcI C'TryA_G Cuts at: 826 1045 2022 2785 3920 4601 4792 826 Size: 219 977 763 1135 681 191 1471 Fragments arranged by size: 1471 1135 977 763 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2524 2764 1872 Size: 652 240 4545 Fragments arranged by size: 4545 652 240 SmlI C'TyrA_G Cuts at: 820 1017 1943 2381 3541 4442 4704 820 Size: 197 926 438 1160 901 262 1553 Fragments arranged by size: 1553 1160 926 901 438 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2518 2795 1132 Size: 1386 277 3774 Fragments arranged by size: 3774 1386 277 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 2394 1517 Size: 877 4560 StyI C'CwwG_G Cuts at: 513 1065 2004 2403 2463 2876 513 Size: 552 939 399 60 413 3074 Fragments arranged by size: 3074 939 552 413 399 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 2683 3464 3481 3634 3819 4264 Size: 1255 639 464 781 17 153 185 445 Cuts at: 4264 325 Size: 1498 Fragments arranged by size: 1498 1255 781 639 464 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2495 2125 Size: 370 5067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2522 2762 1870 Size: 652 240 4545 Fragments arranged by size: 4545 652 240 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI