Restriction map for
pFN21AE0159
(Linear) (Six-base) MAPSORT of: KIEE0159.seq Check: 8095 from: 1 to: 732
KIEE0159.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 732
Size: 579 153
AfeI AGC'GCT
Cuts at: 0 147 732
Size: 147 585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 267 732
Size: 267 465
AlwNI CAG_nnn'CTG
Cuts at: 0 489 602 732
Size: 489 113 130
Fragments arranged by size:
489 130 113
ApaI G_GGCC'C
Cuts at: 0 96 732
Size: 96 636
ApoI r'AATT_y
Cuts at: 0 616 732
Size: 616 116
AscI GG'CGCG_CC
Cuts at: 0 28 732
Size: 28 704
AvaI C'yCGr_G
Cuts at: 0 517 732
Size: 517 215
BanII G_rGCy'C
Cuts at: 0 96 670 732
Size: 96 574 62
Fragments arranged by size:
574 96 62
BbvCI CC'TCA_GC
Cuts at: 0 206 732
Size: 206 526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 104 732
Size: 70 34 628
Fragments arranged by size:
628 70 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 549 732
Size: 549 183
Bme1580I G_kGCm'C
Cuts at: 0 96 732
Size: 96 636
BmtI G_CTAG'C
Cuts at: 0 152 732
Size: 152 580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 336 732
Size: 281 55 396
Fragments arranged by size:
396 281 55
Bpu10I CC'TnA_GC
Cuts at: 0 206 669 732
Size: 206 463 63
Fragments arranged by size:
463 206 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 732
Size: 361 371
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 732
Size: 583 30 119
Fragments arranged by size:
583 119 30
BseYI C'CCAG_C
Cuts at: 0 493 720 732
Size: 493 227 12
Fragments arranged by size:
493 227 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 732
Size: 100 632
BsiEI CG_ry'CG
Cuts at: 0 664 732
Size: 664 68
BsiHKAI G_wGCw'C
Cuts at: 0 542 670 732
Size: 542 128 62
Fragments arranged by size:
542 128 62
Bsp1286I G_dGCh'C
Cuts at: 0 96 542 670 732
Size: 96 446 128 62
Fragments arranged by size:
446 128 96 62
BsrDI GCAATG_nn'
Cuts at: 0 285 732
Size: 285 447
BssHII G'CGCG_C
Cuts at: 0 28 732
Size: 28 704
BstAPI GCAn_nnn'nTGC
Cuts at: 0 415 732
Size: 415 317
BstEII G'GTnAC_C
Cuts at: 0 546 690 732
Size: 546 144 42
Fragments arranged by size:
546 144 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 732
Size: 360 372
Bsu36I CC'TnA_GG
Cuts at: 0 584 732
Size: 584 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 516 732
Size: 516 216
EaeI y'GGCC_r
Cuts at: 0 455 732
Size: 455 277
EarI CTCTTCn'nnn_
Cuts at: 0 131 224 732
Size: 131 93 508
Fragments arranged by size:
508 131 93
EcoICRI GAG'CTC
Cuts at: 0 668 732
Size: 668 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 336 579 732
Size: 281 55 243 153
Fragments arranged by size:
281 243 153 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 682 732
Size: 682 50
EcoRI G'AATT_C
Cuts at: 0 616 732
Size: 616 116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 315 732
Size: 315 417
HaeII r_GCGC'y
Cuts at: 0 62 149 732
Size: 62 87 583
Fragments arranged by size:
583 87 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 732
Size: 583 32 117
Fragments arranged by size:
583 117 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 732
Size: 192 540
MscI TGG'CCA
Cuts at: 0 457 732
Size: 457 275
MslI CAynn'nnrTG
Cuts at: 0 535 732
Size: 535 197
MspA1I CmG'CkG
Cuts at: 0 506 732
Size: 506 226
NdeI CA'TA_TG
Cuts at: 0 334 732
Size: 334 398
NheI G'CTAG_C
Cuts at: 0 148 732
Size: 148 584
NspI r_CATG'y
Cuts at: 0 513 732
Size: 513 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 489 732
Size: 489 243
PfoI T'CCnGG_A
Cuts at: 0 601 732
Size: 601 131
PspOMI G'GGCC_C
Cuts at: 0 92 732
Size: 92 640
PstI C_TGCA'G
Cuts at: 0 21 732
Size: 21 711
SacI G_AGCT'C
Cuts at: 0 670 732
Size: 670 62
SexAI A'CCwGG_T
Cuts at: 0 194 732
Size: 194 538
SfcI C'TryA_G
Cuts at: 0 17 732
Size: 17 715
SmaI CCC'GGG
Cuts at: 0 519 732
Size: 519 213
SmlI C'TyrA_G
Cuts at: 0 376 732
Size: 376 356
SphI G_CATG'C
Cuts at: 0 513 732
Size: 513 219
StuI AGG'CCT
Cuts at: 0 389 732
Size: 389 343
StyI C'CwwG_G
Cuts at: 0 398 458 732
Size: 398 60 274
Fragments arranged by size:
398 274 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 678 732
Size: 214 464 54
Fragments arranged by size:
464 214 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 490 732
Size: 120 370 242
Fragments arranged by size:
370 242 120
XmaI C'CCGG_G
Cuts at: 0 517 732
Size: 517 215
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI ApoI AscI AvaI
BanII BbvCI BcgI BciVI Bme1580I BmtI BpmI Bpu10I
BpuEI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI
BssHII BstAPI BstEII BstXI Bsu36I BtgZI EaeI EarI
EcoICRI Eco57MI EcoNI EcoRI FalI HaeII Hin4I MmeI
MscI MslI MspA1I NdeI NheI NspI PflMI PfoI
PspOMI PstI SacI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstBI BstYI BstZ17I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoO109I EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI