(Circular) (Six-base) MAPSORT of: pFN21AE0474.seq Check: 9307 from: 1 to: 5206 pFN21AE0474 5206 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3016 278 Size: 53 83 186 936 1480 2468 Fragments arranged by size: 2468 1480 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2549 1937 Size: 612 4594 Acc65I G'GTAC_C Cuts at: 2527 2527 Size: 5206 AclI AA'CG_TT Cuts at: 1563 3333 3706 1563 Size: 1770 373 3063 Fragments arranged by size: 3063 1770 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2458 3269 4653 4921 1632 Size: 826 811 1384 268 1917 Fragments arranged by size: 1917 1384 826 811 268 AflII C'TTAA_G Cuts at: 820 1017 2105 820 Size: 197 1088 3921 Fragments arranged by size: 3921 1088 197 AflIII A'CryG_T Cuts at: 1203 4105 5082 1203 Size: 2902 977 1327 Fragments arranged by size: 2902 1327 977 AgeI A'CCGG_T Cuts at: 4037 4037 Size: 5206 AhdI GACnn_n'nnGTC Cuts at: 3935 3935 Size: 5206 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2325 1184 Size: 1141 4065 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2172 2204 2172 Size: 32 5174 AlwNI CAG_nnn'CTG Cuts at: 2021 2949 4521 2021 Size: 928 1572 2706 Fragments arranged by size: 2706 1572 928 ApaLI G'TGCA_C Cuts at: 3263 4419 3263 Size: 1156 4050 ApoI r'AATT_y Cuts at: 1493 2267 2515 2741 2893 5002 1493 Size: 774 248 226 152 2109 1697 Fragments arranged by size: 2109 1697 774 248 226 152 AseI AT'TA_AT Cuts at: 160 1032 3760 160 Size: 872 2728 1606 Fragments arranged by size: 2728 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5206 AvaI C'yCGr_G Cuts at: 1870 1943 2531 1870 Size: 73 588 4545 Fragments arranged by size: 4545 588 73 BamHI G'GATC_C Cuts at: 2536 2903 2536 Size: 367 4839 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2527 3982 618 Size: 325 217 627 539 201 1455 1842 Fragments arranged by size: 1842 1455 627 539 325 217 201 BanII G_rGCy'C Cuts at: 1391 2218 2525 1391 Size: 827 307 4072 Fragments arranged by size: 4072 827 307 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3397 3431 1903 Size: 34 1460 34 3678 Fragments arranged by size: 3678 1460 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3098 4308 3098 Size: 1210 3996 BclI T'GATC_A Cuts at: 1548 1548 Size: 5206 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3817 136 Size: 107 122 71 631 605 2145 1525 Fragments arranged by size: 2145 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2028 5201 2028 Size: 3173 2033 BlpI GC'TnA_GC Cuts at: 2623 2623 Size: 5206 Bme1580I G_kGCm'C Cuts at: 1790 2329 3267 4423 1790 Size: 539 938 1156 2573 Fragments arranged by size: 2573 1156 938 539 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5206 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2937 3895 442 Size: 416 53 83 399 24 1520 958 1753 Fragments arranged by size: 1753 1520 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2148 3866 1239 Size: 909 1718 2579 Fragments arranged by size: 2579 1718 909 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3331 4196 4494 3331 Size: 865 298 4043 Fragments arranged by size: 4043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3869 882 Size: 617 2370 2219 Fragments arranged by size: 2370 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2997 493 Size: 711 1793 2702 Fragments arranged by size: 2702 1793 711 BsaBI GATnn'nnATC Cuts at: 5200 5200 Size: 5206 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3013 3395 4030 275 Size: 53 83 186 936 1480 382 635 1451 Fragments arranged by size: 1480 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3639 4037 4311 4458 4869 3639 Size: 398 274 147 411 3976 Fragments arranged by size: 3976 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4981 Fragments arranged by size: 4981 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5206 BseYI C'CCAG_C Cuts at: 4409 4905 4409 Size: 496 4710 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4633 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2656 3417 4445 1540 Size: 275 186 655 761 1028 2301 Fragments arranged by size: 2301 1028 761 655 275 186 BsiHKAI G_wGCw'C Cuts at: 2218 3267 3352 4423 2218 Size: 1049 85 1071 3001 Fragments arranged by size: 3001 1071 1049 85 BsmI GAATG_Cn' Cuts at: 2717 2810 2717 Size: 93 5113 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 2329 2525 3267 3352 4423 1391 Size: 399 428 111 196 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 428 399 196 111 85 BspEI T'CCGG_A Cuts at: 4869 4869 Size: 5206 BspHI T'CATG_A Cuts at: 3095 4825 3095 Size: 1730 3476 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 844 Size: 1717 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3093 1394 Size: 429 1270 3507 Fragments arranged by size: 3507 1270 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2491 3701 3875 65 Size: 1183 1243 1210 174 1396 Fragments arranged by size: 1396 1243 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3850 4037 1700 Size: 110 2040 187 2869 Fragments arranged by size: 2869 2040 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5206 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5206 BssSI C'ACGA_G Cuts at: 3266 4278 3266 Size: 1012 4194 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5206 BstBI TT'CG_AA Cuts at: 4010 4010 Size: 5206 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5206 BstYI r'GATC_y Cuts at: 1972 2028 2536 2903 3288 3305 4757 4843 4855 Size: 56 508 367 385 17 1452 86 12 Cuts at: 4855 5201 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 508 385 367 346 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4654 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4467 Fragments arranged by size: 4467 683 56 BtsI GCAGTG_nn' Cuts at: 2304 2722 3516 3536 2304 Size: 418 794 20 3974 Fragments arranged by size: 3974 794 418 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5206 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5171 DraI TTT'AAA Cuts at: 2510 2510 Size: 5206 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4213 809 Size: 507 2897 1802 Fragments arranged by size: 2897 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2198 2653 3542 Size: 54 1425 50 275 18 368 455 889 Cuts at: 3542 5171 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 455 368 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2653 1537 Size: 275 841 4090 Fragments arranged by size: 4090 841 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 3136 1353 Size: 918 865 3423 Fragments arranged by size: 3423 918 865 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3793 4167 4313 387 Size: 1064 138 2204 374 146 1280 Fragments arranged by size: 2204 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2216 2216 Size: 5206 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2458 3269 3866 4653 4921 1239 Size: 393 516 310 811 597 787 268 1524 Fragments arranged by size: 1524 811 787 597 516 393 310 268 EcoRI G'AATT_C Cuts at: 2515 2893 2515 Size: 378 4828 FspI TGC'GCA Cuts at: 3712 3712 Size: 5206 HaeII r_GCGC'y Cuts at: 1678 4353 1678 Size: 2675 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3847 3879 3921 3953 1373 Size: 32 135 32 2275 32 42 32 2626 Fragments arranged by size: 2626 2275 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2550 2796 669 Size: 988 281 612 246 3079 Fragments arranged by size: 3079 988 612 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5206 HpaI GTT'AAC Cuts at: 2796 2796 Size: 5206 KpnI G_GTAC'C Cuts at: 2531 2531 Size: 5206 MfeI C'AATT_G Cuts at: 2805 2805 Size: 5206 MluI A'CGCG_T Cuts at: 5082 5082 Size: 5206 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4320 4504 1305 Size: 3015 184 2007 Fragments arranged by size: 3015 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2200 10 Size: 54 1425 343 368 3016 Fragments arranged by size: 3016 1425 368 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2496 3164 3523 3682 518 Size: 648 1330 668 359 159 2042 Fragments arranged by size: 2042 1330 668 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2431 2622 3299 4447 4692 1606 Size: 825 191 677 1148 245 2120 Fragments arranged by size: 2120 1148 825 677 245 191 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4654 NdeI CA'TA_TG Cuts at: 387 2152 387 Size: 1765 3441 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5206 NotI GC'GGCC_GC Cuts at: 2653 2653 Size: 5206 NspI r_CATG'y Cuts at: 1132 1229 2047 2564 4109 1132 Size: 97 818 517 1545 2229 Fragments arranged by size: 2229 1545 818 517 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5206 PciI A'CATG_T Cuts at: 4105 4105 Size: 5206 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5206 PmeI GTTT'AAAC Cuts at: 2510 2510 Size: 5206 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3275 3307 4788 4820 3275 Size: 32 1481 32 3661 Fragments arranged by size: 3661 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5206 PsiI TTA'TAA Cuts at: 2393 2776 2393 Size: 383 4823 PstI C_TGCA'G Cuts at: 830 2558 830 Size: 1728 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5206 PvuII CAG'CTG Cuts at: 2431 2431 Size: 5206 SacI G_AGCT'C Cuts at: 2218 2218 Size: 5206 SalI G'TCGA_C Cuts at: 1936 2548 1936 Size: 612 4594 SapI GCTCTTCn'nnn_ Cuts at: 2271 2271 Size: 5206 SbfI CC_TGCA'GG Cuts at: 2558 2558 Size: 5206 ScaI AGT'ACT Cuts at: 3454 5166 3454 Size: 1712 3494 SfcI C'TryA_G Cuts at: 826 1045 2554 3689 4370 4561 826 Size: 219 1509 1135 681 191 1471 Fragments arranged by size: 1509 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5206 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5206 SmaI CCC'GGG Cuts at: 1872 2533 1872 Size: 661 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 3310 4211 4473 820 Size: 197 926 162 1205 901 262 1553 Fragments arranged by size: 1553 1205 926 901 262 197 162 SnaBI TAC'GTA Cuts at: 493 2997 493 Size: 2504 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5206 SphI G_CATG'C Cuts at: 1132 2564 1132 Size: 1432 3774 SspI AAT'ATT Cuts at: 5 52 3130 5 Size: 47 3078 2081 Fragments arranged by size: 3078 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5206 StyI C'CwwG_G Cuts at: 513 1065 2645 513 Size: 552 1580 3074 Fragments arranged by size: 3074 1580 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3233 3250 3403 3588 4033 325 Size: 1255 1653 17 153 185 445 1498 Fragments arranged by size: 1653 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3452 5164 96 Size: 274 80 33 51 1443 1475 1712 138 Fragments arranged by size: 1712 1475 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5166 5198 5166 Size: 32 5174 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5206 XbaI T'CTAG_A Cuts at: 2191 2542 2191 Size: 351 4855 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5206 XmaI C'CCGG_G Cuts at: 1870 2531 1870 Size: 661 4545 XmnI GAAnn'nnTTC Cuts at: 3335 3335 Size: 5206 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3014 276 Size: 53 83 186 936 1480 2468 Fragments arranged by size: 2468 1480 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI