(Circular) (Six-base) MAPSORT of: pFN21AB6532.seq Check: 8092 from: 1 to: 5872 pFN21AB6532 5872 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2257 2616 3215 1937 Size: 320 359 599 4594 Fragments arranged by size: 4594 599 359 320 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 2771 3999 4372 1563 Size: 1208 1228 373 3063 Fragments arranged by size: 3063 1228 1208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2015 2165 2220 2300 2730 3069 3935 5319 Size: 383 150 55 80 430 339 866 1384 Cuts at: 5319 5587 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 866 430 383 339 268 150 80 55 AflII C'TTAA_G Cuts at: 820 1017 2081 820 Size: 197 1064 4611 Fragments arranged by size: 4611 1064 197 AflIII A'CryG_T Cuts at: 1203 2367 4771 5748 1203 Size: 1164 2404 977 1327 Fragments arranged by size: 2404 1327 1164 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2066 1184 Size: 882 4990 AlwNI CAG_nnn'CTG Cuts at: 3615 5187 3615 Size: 1572 4300 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 2207 3181 3407 3559 5668 1493 Size: 714 974 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 714 226 152 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 3197 1870 Size: 73 1254 4545 Fragments arranged by size: 4545 1254 73 BamHI G'GATC_C Cuts at: 2350 2860 3202 3569 2350 Size: 510 342 367 4653 Fragments arranged by size: 4653 510 367 342 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3193 4648 618 Size: 325 217 627 1406 1455 1842 Fragments arranged by size: 1842 1455 1406 627 325 217 BanII G_rGCy'C Cuts at: 1391 3191 1391 Size: 1800 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3105 928 Size: 971 1206 3695 Fragments arranged by size: 3695 1206 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 2646 2829 3076 1548 Size: 1098 183 247 4344 Fragments arranged by size: 4344 1098 247 183 BfrBI ATG'CAT Cuts at: 2688 2688 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3028 5867 3028 Size: 2839 3033 BlpI GC'TnA_GC Cuts at: 3289 3289 Size: 5872 Bme1580I G_kGCm'C Cuts at: 1790 3933 5089 1790 Size: 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3603 4561 442 Size: 416 53 83 399 24 2186 958 1753 Fragments arranged by size: 2186 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2571 1056 Size: 1515 4357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3164 4532 1239 Size: 1925 1368 2579 Fragments arranged by size: 2579 1925 1368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3997 4862 5160 3997 Size: 865 298 4709 Fragments arranged by size: 4709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5647 Fragments arranged by size: 5647 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5872 BseYI C'CCAG_C Cuts at: 5075 5571 5075 Size: 496 5376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 3322 4083 5111 1540 Size: 275 186 76 1245 761 1028 2301 Fragments arranged by size: 2301 1245 1028 761 275 186 76 BsiHKAI G_wGCw'C Cuts at: 2641 3933 4018 5089 2641 Size: 1292 85 1071 3424 Fragments arranged by size: 3424 1292 1071 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2641 3191 3933 4018 5089 1391 Size: 399 851 550 742 85 1071 2174 Fragments arranged by size: 2174 1071 851 742 550 399 85 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 2325 2374 3079 3105 3761 5491 2325 Size: 49 705 26 656 1730 2706 Fragments arranged by size: 2706 1730 705 656 49 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 3044 3227 844 Size: 2101 99 183 3489 Fragments arranged by size: 3489 2101 183 99 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4367 4541 65 Size: 1183 3119 174 1396 Fragments arranged by size: 3119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 2556 3932 4944 2556 Size: 1376 1012 3484 Fragments arranged by size: 3484 1376 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3071 794 Size: 2277 3595 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstYI r'GATC_y Cuts at: 1972 2350 2427 2860 3028 3202 3569 3954 3971 Size: 378 77 433 168 174 367 385 17 Cuts at: 3971 5423 5509 5521 5867 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 433 385 378 367 346 174 168 86 77 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4381 Fragments arranged by size: 4381 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5133 Fragments arranged by size: 5133 683 56 BtsI GCAGTG_nn' Cuts at: 3388 4182 4202 3388 Size: 794 20 5058 Fragments arranged by size: 5058 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 3319 4208 Size: 54 1425 50 275 18 244 1245 889 Cuts at: 4208 5837 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1245 889 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2074 3319 1537 Size: 275 262 1245 4090 Fragments arranged by size: 4090 1245 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 3802 1353 Size: 2449 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4459 4833 4979 387 Size: 1064 138 435 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2165 2220 2300 2730 3069 3164 Size: 393 383 150 55 80 430 339 95 Cuts at: 3164 3935 4532 5319 5587 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 430 393 383 339 268 150 95 80 55 EcoNI CCTnn'n_nnAGG Cuts at: 2505 2505 Size: 5872 EcoO109I rG'GnC_Cy Cuts at: 2149 2380 2957 2149 Size: 231 577 5064 Fragments arranged by size: 5064 577 231 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2316 2316 Size: 5872 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2030 5019 1678 Size: 352 2989 2531 Fragments arranged by size: 2989 2531 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373 Size: 32 135 32 2941 32 42 32 2626 Fragments arranged by size: 2941 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2196 2258 3216 3462 669 Size: 988 281 258 62 958 246 3079 Fragments arranged by size: 3079 988 958 281 258 246 62 HindIII A'AGCT_T Cuts at: 748 3002 748 Size: 2254 3618 HpaI GTT'AAC Cuts at: 2196 3462 2196 Size: 1266 4606 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 2994 3471 2994 Size: 477 5395 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4986 5170 1305 Size: 3681 184 2007 Fragments arranged by size: 3681 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 3830 4189 4348 518 Size: 648 1530 1134 359 159 2042 Fragments arranged by size: 2042 1530 1134 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2575 2750 3288 3965 5113 5358 1606 Size: 969 175 538 677 1148 245 2120 Fragments arranged by size: 2120 1148 969 677 538 245 175 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4381 Fragments arranged by size: 4381 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 2567 1052 Size: 1515 4357 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NsiI A_TGCA'T Cuts at: 2690 2690 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 2371 3230 4775 1132 Size: 97 1142 859 1545 2229 Fragments arranged by size: 2229 1545 1142 859 97 PasI CC'CwG_GG Cuts at: 1348 2384 1348 Size: 1036 4836 PciI A'CATG_T Cuts at: 2367 4771 2367 Size: 2404 3468 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5872 PfoI T'CCnGG_A Cuts at: 2430 2878 2430 Size: 448 5424 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2957 2380 Size: 577 5295 PshAI GACnn'nnGTC Cuts at: 1613 2076 1613 Size: 463 5409 PsiI TTA'TAA Cuts at: 2364 3442 2364 Size: 1078 4794 PstI C_TGCA'G Cuts at: 830 3224 830 Size: 2394 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2575 2750 2575 Size: 175 5697 SalI G'TCGA_C Cuts at: 1936 2256 3214 1936 Size: 320 958 4594 Fragments arranged by size: 4594 958 320 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SexAI A'CCwGG_T Cuts at: 2530 2530 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 2153 3220 4355 5036 5227 826 Size: 219 1108 1067 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 1067 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 3976 4877 5139 820 Size: 197 926 138 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 138 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2004 3311 513 Size: 552 939 1307 3074 Fragments arranged by size: 3074 1307 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 3899 3916 4069 4254 4699 325 Size: 1255 585 1734 17 153 185 445 1498 Fragments arranged by size: 1734 1498 1255 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2622 3044 3091 Size: 274 80 33 51 1443 645 422 47 Cuts at: 3091 4118 5830 96 Size: 1027 1712 138 Fragments arranged by size: 1712 1443 1027 645 422 274 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI