Restriction map for
pFN21AB6588
(Linear) (Six-base) MAPSORT of: KIBB6588.seq Check: 6685 from: 1 to: 1218
KIBB6588.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 290 392 1218
Size: 290 102 826
Fragments arranged by size:
826 290 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1091 1218
Size: 293 798 127
Fragments arranged by size:
798 293 127
AflIII A'CryG_T
Cuts at: 0 1193 1218
Size: 1193 25
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1109 1141 1218
Size: 1109 32 77
Fragments arranged by size:
1109 77 32
AlwNI CAG_nnn'CTG
Cuts at: 0 195 580 1069 1096 1218
Size: 195 385 489 27 122
Fragments arranged by size:
489 385 195 122 27
ApaLI G'TGCA_C
Cuts at: 0 1086 1218
Size: 1086 132
ApoI r'AATT_y
Cuts at: 0 280 664 946 1218
Size: 280 384 282 272
Fragments arranged by size:
384 282 280 272
AvaI C'yCGr_G
Cuts at: 0 599 1016 1218
Size: 599 417 202
Fragments arranged by size:
599 417 202
BanII G_rGCy'C
Cuts at: 0 211 521 1049 1218
Size: 211 310 528 169
Fragments arranged by size:
528 310 211 169
BbsI GAAGACnn'nnnn_
Cuts at: 0 592 1218
Size: 592 626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 173 1218
Size: 139 34 1045
Fragments arranged by size:
1045 139 34
BlpI GC'TnA_GC
Cuts at: 0 542 1043 1218
Size: 542 501 175
Fragments arranged by size:
542 501 175
Bme1580I G_kGCm'C
Cuts at: 0 1090 1218
Size: 1090 128
BmgBI CAC'GTC
Cuts at: 0 294 1218
Size: 294 924
BmrI ACTGGGnnnn_n'
Cuts at: 0 541 590 1218
Size: 541 49 628
Fragments arranged by size:
628 541 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 225 1218
Size: 225 993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 76 557 796 809 841 1218
Size: 52 24 481 239 13 32 377
Fragments arranged by size:
481 377 239 52 32 24 13
Bpu10I CC'TnA_GC
Cuts at: 0 515 1218
Size: 515 703
BsaBI GATnn'nnATC
Cuts at: 0 895 1218
Size: 895 323
BsaWI w'CCGG_w
Cuts at: 0 125 1218
Size: 125 1093
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 196 756 979 1218
Size: 49 147 560 223 239
Fragments arranged by size:
560 239 223 147 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 826 1218
Size: 352 474 392
Fragments arranged by size:
474 392 352
BsiHKAI G_wGCw'C
Cuts at: 0 521 1049 1090 1218
Size: 521 528 41 128
Fragments arranged by size:
528 521 128 41
Bsp1286I G_dGCh'C
Cuts at: 0 211 521 1049 1090 1218
Size: 211 310 528 41 128
Fragments arranged by size:
528 310 211 128 41
BspEI T'CCGG_A
Cuts at: 0 125 1218
Size: 125 1093
BsrDI GCAATG_nn'
Cuts at: 0 536 1218
Size: 536 682
BstBI TT'CG_AA
Cuts at: 0 524 1218
Size: 524 694
BstYI r'GATC_y
Cuts at: 0 374 1218
Size: 374 844
BtgI C'CryG_G
Cuts at: 0 236 1218
Size: 236 982
BtsI GCAGTG_nn'
Cuts at: 0 910 1218
Size: 910 308
EarI CTCTTCn'nnn_
Cuts at: 0 526 1218
Size: 526 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 1218
Size: 49 1169
EcoICRI GAG'CTC
Cuts at: 0 519 1047 1218
Size: 519 528 171
Fragments arranged by size:
528 519 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 76 293 557 796 809 841 1091
Size: 52 24 217 264 239 13 32 250
Cuts at: 1091 1218
Size: 127
Fragments arranged by size:
264 250 239 217 127 52 32 24
13
EcoRI G'AATT_C
Cuts at: 0 946 1218
Size: 946 272
EcoRV GAT'ATC
Cuts at: 0 897 1218
Size: 897 321
HaeII r_GCGC'y
Cuts at: 0 7 1218
Size: 7 1211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1013 1115 1147 1218
Size: 981 32 102 32 71
Fragments arranged by size:
981 102 71 32 32
MfeI C'AATT_G
Cuts at: 0 330 1218
Size: 330 888
MslI CAynn'nnrTG
Cuts at: 0 739 1218
Size: 739 479
MspA1I CmG'CkG
Cuts at: 0 53 96 192 1218
Size: 53 43 96 1026
Fragments arranged by size:
1026 96 53 43
NcoI C'CATG_G
Cuts at: 0 236 1218
Size: 236 982
NspI r_CATG'y
Cuts at: 0 724 1104 1197 1218
Size: 724 380 93 21
Fragments arranged by size:
724 380 93 21
PasI CC'CwG_GG
Cuts at: 0 922 1218
Size: 922 296
PciI A'CATG_T
Cuts at: 0 1193 1218
Size: 1193 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 580 1218
Size: 580 638
PfoI T'CCnGG_A
Cuts at: 0 899 1218
Size: 899 319
PstI C_TGCA'G
Cuts at: 0 137 200 1218
Size: 137 63 1018
Fragments arranged by size:
1018 137 63
PvuII CAG'CTG
Cuts at: 0 96 192 1218
Size: 96 96 1026
Fragments arranged by size:
1026 96 96
SacI G_AGCT'C
Cuts at: 0 521 1049 1218
Size: 521 528 169
Fragments arranged by size:
528 521 169
SapI GCTCTTCn'nnn_
Cuts at: 0 526 1218
Size: 526 692
SfcI C'TryA_G
Cuts at: 0 133 187 196 1218
Size: 133 54 9 1022
Fragments arranged by size:
1022 133 54 9
SmaI CCC'GGG
Cuts at: 0 601 1018 1218
Size: 601 417 200
Fragments arranged by size:
601 417 200
SrfI GCCC'GGGC
Cuts at: 0 601 1218
Size: 601 617
SspI AAT'ATT
Cuts at: 0 67 1218
Size: 67 1151
StyI C'CwwG_G
Cuts at: 0 236 548 1163 1218
Size: 236 312 615 55
Fragments arranged by size:
615 312 236 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 342 1218
Size: 342 876
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 1218
Size: 336 882
XmaI C'CCGG_G
Cuts at: 0 599 1016 1218
Size: 599 417 202
Fragments arranged by size:
599 417 202
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApaLI ApoI AvaI
BanII BbsI BcgI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaBI BsaWI BseRI BsgI BsiHKAI Bsp1286I
BspEI BsrDI BstBI BstYI BtgI BtsI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV HaeII Hin4I MfeI MslI
MspA1I NcoI NspI PasI PciI PflMI PfoI PstI
PvuII SacI SapI SfcI SmaI SrfI SspI StyI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BmtI BpuEI BsaI BsaAI BsaHI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EcoNI EcoO109I FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI