(Circular) (Six-base) MAPSORT of: pFN21AB6395.seq Check: 1878 from: 1 to: 5533 pFN21AB6395 5533 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 1937 Size: 939 4594 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 2196 3660 4033 1563 Size: 633 1464 373 3063 Fragments arranged by size: 3063 1464 633 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 3596 4980 5248 1632 Size: 1056 908 1384 268 1917 Fragments arranged by size: 1917 1384 1056 908 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 4432 5409 1203 Size: 3229 977 1327 Fragments arranged by size: 3229 1327 977 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 2173 3276 4848 2173 Size: 1103 1572 2858 Fragments arranged by size: 2858 1572 1103 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2159 2842 3068 3220 5329 1493 Size: 666 683 226 152 2109 1697 Fragments arranged by size: 2109 1697 683 666 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2139 2274 2858 1870 Size: 73 91 105 135 584 4545 Fragments arranged by size: 4545 584 135 105 91 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2219 2348 2854 4309 618 Size: 325 217 627 432 129 506 1455 1842 Fragments arranged by size: 1842 1455 627 506 432 325 217 129 BanII G_rGCy'C Cuts at: 1391 2032 2130 2852 1391 Size: 641 98 722 4072 Fragments arranged by size: 4072 722 641 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4562 BbvCI CC'TCA_GC Cuts at: 2281 2281 Size: 5533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4144 136 Size: 107 122 71 631 605 2472 1525 Fragments arranged by size: 2472 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2950 2950 Size: 5533 Bme1580I G_kGCm'C Cuts at: 1790 3594 4750 1790 Size: 1804 1156 2573 Fragments arranged by size: 2573 1804 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 3264 4222 Size: 416 53 83 399 24 975 872 958 Cuts at: 4222 442 Size: 1753 Fragments arranged by size: 1753 975 958 872 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2106 2751 4193 1239 Size: 867 645 1442 2579 Fragments arranged by size: 2579 1442 867 645 Bpu10I CC'TnA_GC Cuts at: 2281 2281 Size: 5533 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4196 882 Size: 617 2697 2219 Fragments arranged by size: 2697 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 3324 493 Size: 711 1356 764 2702 Fragments arranged by size: 2702 1356 764 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2080 3340 3722 4357 Size: 53 83 186 936 547 1260 382 635 Cuts at: 4357 275 Size: 1451 Fragments arranged by size: 1451 1260 936 635 547 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2724 2754 1178 Size: 30 165 30 1321 30 3957 Fragments arranged by size: 3957 1321 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5533 BseYI C'CCAG_C Cuts at: 2285 2529 4736 5232 2285 Size: 244 2207 496 2586 Fragments arranged by size: 2586 2207 496 244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2385 2792 1163 Size: 573 649 407 3904 Fragments arranged by size: 3904 649 573 407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2983 3744 4772 1540 Size: 275 186 47 935 761 1028 2301 Fragments arranged by size: 2301 1028 935 761 275 186 47 BsiHKAI G_wGCw'C Cuts at: 2130 3594 3679 4750 2130 Size: 1464 85 1071 2913 Fragments arranged by size: 2913 1464 1071 85 BsmI GAATG_Cn' Cuts at: 2151 2411 3044 3137 2151 Size: 260 633 93 4547 Fragments arranged by size: 4547 633 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2130 2852 3594 3679 4750 1391 Size: 399 242 98 722 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 722 399 242 98 85 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 3422 5152 3422 Size: 1730 3803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2349 2811 2888 844 Size: 1505 462 77 3489 Fragments arranged by size: 3489 1505 462 77 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528 Size: 891 367 385 17 1452 86 12 346 Cuts at: 5528 1972 Size: 1977 Fragments arranged by size: 1977 1452 891 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2215 2601 513 Size: 552 939 211 386 3445 Fragments arranged by size: 3445 939 552 386 211 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4794 Fragments arranged by size: 4794 683 56 BtsI GCAGTG_nn' Cuts at: 2271 3049 3843 3863 2271 Size: 778 794 20 3941 Fragments arranged by size: 3941 794 778 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2397 2686 Size: 54 1425 50 275 18 215 352 289 Cuts at: 2686 2980 3869 5498 8 Size: 294 889 1629 43 Fragments arranged by size: 1629 1425 889 352 294 289 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 2980 1537 Size: 275 233 935 4090 Fragments arranged by size: 4090 935 275 233 EarI CTCTTCn'nnn_ Cuts at: 1353 2664 3463 1353 Size: 1311 799 3423 Fragments arranged by size: 3423 1311 799 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4120 4494 4640 387 Size: 1064 138 2531 374 146 1280 Fragments arranged by size: 2531 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2128 2128 Size: 5533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2688 2751 3596 4193 4980 5248 Size: 393 474 582 63 845 597 787 268 Cuts at: 5248 1239 Size: 1524 Fragments arranged by size: 1524 845 787 597 582 474 393 268 63 EcoO109I rG'GnC_Cy Cuts at: 2289 2543 2289 Size: 254 5279 EcoRI G'AATT_C Cuts at: 2159 2842 3220 2159 Size: 683 378 4472 Fragments arranged by size: 4472 683 378 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2012 2392 4680 1678 Size: 334 380 2288 2531 Fragments arranged by size: 2531 2288 380 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2724 2756 4174 4206 4248 Size: 32 135 32 1152 32 1418 32 42 Cuts at: 4248 4280 1373 Size: 32 2626 Fragments arranged by size: 2626 1418 1152 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 4647 4831 1305 Size: 1191 2151 184 2007 Fragments arranged by size: 2151 2007 1191 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 10 Size: 54 1425 343 567 3144 Fragments arranged by size: 3144 1425 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3491 3850 4009 518 Size: 648 2325 359 159 2042 Fragments arranged by size: 2325 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2285 2949 3626 4774 5019 1606 Size: 438 241 664 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 438 245 241 NcoI C'CATG_G Cuts at: 513 1065 2004 2215 513 Size: 552 939 211 3831 Fragments arranged by size: 3831 939 552 211 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2891 4436 1132 Size: 97 1662 1545 2229 Fragments arranged by size: 2229 1662 1545 97 PasI CC'CwG_GG Cuts at: 1348 2173 1348 Size: 825 4708 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 2173 1108 Size: 1065 4468 PfoI T'CCnGG_A Cuts at: 2545 2545 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2289 2543 2289 Size: 254 5279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PstI C_TGCA'G Cuts at: 830 2885 830 Size: 2055 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2285 2285 Size: 5533 SacI G_AGCT'C Cuts at: 2130 2130 Size: 5533 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 2381 3781 5493 2381 Size: 1400 1712 2421 Fragments arranged by size: 2421 1712 1400 SfcI C'TryA_G Cuts at: 826 1045 2101 2881 4016 4697 4888 826 Size: 219 1056 780 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 780 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2141 2860 1872 Size: 269 719 4545 Fragments arranged by size: 4545 719 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 4538 4800 820 Size: 197 926 1694 901 262 1553 Fragments arranged by size: 1694 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 2675 1517 Size: 1158 4375 StyI C'CwwG_G Cuts at: 513 1065 2004 2215 2400 2683 2972 513 Size: 552 939 211 185 283 289 3074 Fragments arranged by size: 3074 939 552 289 283 211 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 2761 3560 3577 3730 3915 4360 Size: 1255 585 596 799 17 153 185 445 Cuts at: 4360 325 Size: 1498 Fragments arranged by size: 1498 1255 799 596 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 2415 2786 Size: 274 80 33 51 1443 402 36 371 Cuts at: 2786 3779 5491 96 Size: 993 1712 138 Fragments arranged by size: 1712 1443 993 402 371 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2174 2174 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2139 2858 1870 Size: 269 719 4545 Fragments arranged by size: 4545 719 269 XmnI GAAnn'nnTTC Cuts at: 3662 3662 Size: 5533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI