(Circular) (Six-base) MAPSORT of: pFN21AE0481.seq Check: 5249 from: 1 to: 5962 pFN21AE0481 5962 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2256 2633 3305 1937 Size: 319 377 672 4594 Fragments arranged by size: 4594 672 377 319 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3206 3705 5277 3206 Size: 499 1572 3891 Fragments arranged by size: 3891 1572 499 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3012 3271 3497 3649 5758 1493 Size: 1519 259 226 152 2109 1697 Fragments arranged by size: 2109 1697 1519 259 226 152 AseI AT'TA_AT Cuts at: 160 1032 3108 4516 160 Size: 872 2076 1408 1606 Fragments arranged by size: 2076 1606 1408 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2343 3287 1870 Size: 73 400 944 4545 Fragments arranged by size: 4545 944 400 73 BamHI G'GATC_C Cuts at: 2357 2883 3292 3659 2357 Size: 526 409 367 4660 Fragments arranged by size: 4660 526 409 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2058 3177 3281 1391 Size: 667 1119 104 4072 Fragments arranged by size: 4072 1119 667 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4991 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BfrBI ATG'CAT Cuts at: 2318 2318 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2663 2687 2892 Size: 416 53 83 399 24 1246 24 205 Cuts at: 2892 2966 3693 4651 442 Size: 74 727 958 1753 Fragments arranged by size: 1753 1246 958 727 416 399 205 83 74 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3068 3068 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2443 2456 2508 4622 1239 Size: 1204 13 52 2114 2579 Fragments arranged by size: 2579 2114 1204 52 13 Bpu10I CC'TnA_GC Cuts at: 2445 2445 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 4625 882 Size: 617 978 2148 2219 Fragments arranged by size: 2219 2148 978 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2415 2699 1186 Size: 1229 284 4449 Fragments arranged by size: 4449 1229 284 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2014 1163 Size: 573 278 5111 Fragments arranged by size: 5111 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 3177 3281 4023 4108 5179 1391 Size: 399 268 1119 104 742 85 1071 2174 Fragments arranged by size: 2174 1119 1071 742 399 268 104 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 3317 844 Size: 1256 1217 3489 Fragments arranged by size: 3489 1256 1217 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 3085 4606 4793 1700 Size: 110 382 893 1521 187 2869 Fragments arranged by size: 2869 1521 893 382 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 2905 4022 5034 2905 Size: 1117 1012 3833 Fragments arranged by size: 3833 1117 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2357 2883 3292 3659 4044 4061 5513 5599 Size: 385 526 409 367 385 17 1452 86 Cuts at: 5599 5611 5957 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 526 409 385 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2634 2634 Size: 5962 BtgI C'CryG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 3988 Fragments arranged by size: 3988 939 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2692 2971 3478 4272 4292 2100 Size: 592 279 507 794 20 3770 Fragments arranged by size: 3770 794 592 507 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 2079 3266 2079 Size: 1187 4775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927 Size: 54 1425 50 275 18 1579 889 1629 Cuts at: 5927 8 Size: 43 Fragments arranged by size: 1629 1579 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 1353 Size: 2539 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3137 4549 4923 5069 387 Size: 1064 138 1548 1412 374 146 1280 Fragments arranged by size: 1548 1412 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2443 2456 2508 4025 4622 5409 5677 Size: 393 811 13 52 1517 597 787 268 Cuts at: 5677 1239 Size: 1524 Fragments arranged by size: 1524 1517 811 787 597 393 268 52 13 EcoO109I rG'GnC_Cy Cuts at: 2224 2604 2642 2921 2224 Size: 380 38 279 5265 Fragments arranged by size: 5265 380 279 38 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2791 2791 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2539 2571 3123 Size: 32 135 32 770 32 165 32 552 Cuts at: 3123 3155 4603 4635 4677 4709 1373 Size: 32 1448 32 42 32 2626 Fragments arranged by size: 2626 1448 770 552 165 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3920 4279 4438 518 Size: 648 2754 359 159 2042 Fragments arranged by size: 2754 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3378 4055 5203 5448 1606 Size: 1772 677 1148 245 2120 Fragments arranged by size: 2120 1772 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 3988 Fragments arranged by size: 3988 939 552 483 NdeI CA'TA_TG Cuts at: 387 2248 387 Size: 1861 4101 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NsiI A_TGCA'T Cuts at: 2320 2320 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 3320 4865 1132 Size: 97 2091 1545 2229 Fragments arranged by size: 2229 2091 1545 97 PasI CC'CwG_GG Cuts at: 1348 2478 2757 2856 1348 Size: 1130 279 99 4454 Fragments arranged by size: 4454 1130 279 99 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2604 2642 2921 2224 Size: 380 38 279 5265 Fragments arranged by size: 5265 380 279 38 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2798 2830 2798 Size: 32 5930 PstI C_TGCA'G Cuts at: 830 2641 3314 830 Size: 1811 673 3478 Fragments arranged by size: 3478 1811 673 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2637 3310 4445 5126 5317 826 Size: 219 1592 673 1135 681 191 1471 Fragments arranged by size: 1592 1471 1135 681 673 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2345 3289 1872 Size: 473 944 4545 Fragments arranged by size: 4545 944 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 3401 513 Size: 552 939 483 914 3074 Fragments arranged by size: 3074 939 914 552 483 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2609 2961 3989 4006 4159 4344 4789 Size: 1255 1029 352 1028 17 153 185 445 Cuts at: 4789 325 Size: 1498 Fragments arranged by size: 1498 1255 1029 1028 445 352 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 2693 2972 1316 Size: 1377 279 4306 Fragments arranged by size: 4306 1377 279 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2485 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2343 3287 1870 Size: 473 944 4545 Fragments arranged by size: 4545 944 473 XmnI GAAnn'nnTTC Cuts at: 3117 4091 3117 Size: 974 4988 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI