Restriction map for
pFN21AE0426
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# NO CUT ENZYMES ( 149 enzymes) #
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Acc65I AccII AccIII AclI AfaI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI ClaI CpoI DraIII EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 886 GACGTC GACGT^C
AccI 129 GTCTAC GT^[AC][GT]AC
AcyI 886 GACGTC G[GA]^CG[CT]C
AhdI 349 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 335 GGGCCC GGGCC^C
Asp700I 423 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 349 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 640 CCCGAG C^[CT]CG[GA]G
AvaII 196 GGACC G^G[AT]CC
BamHI 662 GGATCC G^GATCC
BbsI 357 GTCTTC GAAGAC(2/6)
BbvCI 315 CCTCAGC CCTCAGC(-5/-2)
BcnI 666 CCGGG CC^[GC]GG
BlpI 805 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 805 GCTAAGC GC^T[ACGT]AGC
BpuAI 357 GTCTTC GAAGAC(2/6)
BsaHI 886 GACGTC G[GA]^CG[CT]C
BsaI 898 GAGACC GGTCTC(1/5)
BsgI 477 CTGCAC GTGCAG(16/14)
BsmBI 292 GAGACG CGTCTC(1/5)
BsoBI 640 CCCGAG C^[CT]CG[GA]G
BsrFI 481 ACCGGC [GA]^CCGG[CT]
BstYI 662 GGATCC [GA]^GATC[CT]
CelII 805 GCTAAGC GC^T[ACGT]AGC
Cfr10I 481 ACCGGC [GA]^CCGG[CT]
DraI 595 TTTAAA TTT^AAA
DraII 334 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 197 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 470 CCACGG C^C[GA][CT]GG
Eam1105I 349 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 334 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 827 GAATTC G^AATTC
EcoT14I 689 CCAAGG C^C[AT][AT]GG
FauI 947 GCGGG CCCGC(4/6)
HgaI 294 GACGC GACGC(5/10)
Hin1I 886 GACGTC G[GA]^CG[CT]C
HindIII 565 AAGCTT A^AGCTT
MaeIII 884 GTGAC ^GT[ACGT]AC
MflI 662 GGATCC [GA]^GATC[CT]
NciI 666 CCGGG CC^[GC]GG
NspI 504 GCATGT [GA]CATG^[CT]
StyI 689 CCAAGG C^C[AT][AT]GG
Tsp45I 884 GTGAC ^GT[GC]AC
XhoII 662 GGATCC [GA]^GATC[CT]
XmnI 423 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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BanI 484 GGCACC G^G[CT][GA]CC
1025 GGCACC
BseRI 19 CTCCTC GAGGAG(10/8)
1085 CTCCTC
BspMI 297 GCAGGT ACCTGC(4/8)
404 GCAGGT
BsrDI 707 GCAATG GCAATG(2/0)
921 GCAATG
BstF5I 312 CATCC GGATG(2/0)
968 GGATG
Cac8I 96 GCCTGC GC[ACGT]^[ACGT]GC
943 GCCAGC
DpnII 663 GATC ^GATC
699 GATC
FokI 312 CATCC GGATG(9/13)
968 GGATG
HapII 482 CCGG C^CGG
666 CCGG
HpaII 482 CCGG C^CGG
666 CCGG
HphI 669 GGTGA GGTGA(8/7)
792 GGTGA
MaeII 887 ACGT A^CGT
1067 ACGT
MboI 663 GATC ^GATC
699 GATC
MseI 596 TTAA T^TAA
1118 TTAA
MspI 482 CCGG C^CGG
666 CCGG
NdeII 663 GATC ^GATC
699 GATC
PleI 349 GACTC GAGTC(4/5)
622 GACTC
PvuII 515 CAGCTG CAG^CTG
811 CAGCTG
Sau3AI 663 GATC ^GATC
699 GATC
SfaNI 928 GATGC GCATC(5/9)
305 GCATC
TfiI 9 GATTC G^A[AT]TC
1079 GAATC
Tru9I 596 TTAA T^TAA
1118 TTAA
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MboII MnlI MslI MspA1I MvaI
MwoI NlaIII NlaIV PflMI Sau96I
ScrFI TaqI TseI Tsp509I TspRI
TthHB8I Van91I