(Circular) (Six-base) MAPSORT of: pFN21AE0426.seq Check: 4737 from: 1 to: 5854 pFN21AE0426 5854 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2293 2293 Size: 5854 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2895 3664 278 Size: 53 83 186 936 1359 769 2468 Fragments arranged by size: 2468 1359 936 769 186 83 53 AccI GT'mk_AC Cuts at: 1937 2135 3197 1937 Size: 198 1062 4594 Fragments arranged by size: 4594 1062 198 Acc65I G'GTAC_C Cuts at: 3175 3175 Size: 5854 AclI AA'CG_TT Cuts at: 1563 3981 4354 1563 Size: 2418 373 3063 Fragments arranged by size: 3063 2418 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2349 2619 3917 5301 5569 1632 Size: 717 270 1298 1384 268 1917 Fragments arranged by size: 1917 1384 1298 717 270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5657 AflIII A'CryG_T Cuts at: 1203 4753 5730 1203 Size: 3550 977 1327 Fragments arranged by size: 3550 1327 977 AgeI A'CCGG_T Cuts at: 4685 4685 Size: 5854 AhdI GACnn_n'nnGTC Cuts at: 2359 4583 2359 Size: 2224 3630 AleI CACnn'nnGTG Cuts at: 1166 2495 2687 1166 Size: 1329 192 4333 Fragments arranged by size: 4333 1329 192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2705 2949 1184 Size: 1521 244 4089 Fragments arranged by size: 4089 1521 244 AlwNI CAG_nnn'CTG Cuts at: 3597 5169 3597 Size: 1572 4282 ApaI G_GGCC'C Cuts at: 2344 2344 Size: 5854 ApaLI G'TGCA_C Cuts at: 3911 5067 3911 Size: 1156 4698 ApoI r'AATT_y Cuts at: 1493 2657 2780 2832 3163 3389 3541 5650 1493 Size: 1164 123 52 331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 331 226 152 123 52 AseI AT'TA_AT Cuts at: 160 1032 4408 160 Size: 872 3376 1606 Fragments arranged by size: 3376 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 AvaI C'yCGr_G Cuts at: 1870 1943 2645 3179 1870 Size: 73 702 534 4545 Fragments arranged by size: 4545 702 534 73 BamHI G'GATC_C Cuts at: 2667 3184 3551 2667 Size: 517 367 4970 Fragments arranged by size: 4970 517 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2489 3030 3175 4630 618 Size: 325 217 627 702 541 145 1455 1842 Fragments arranged by size: 1842 1455 702 627 541 325 217 145 BanII G_rGCy'C Cuts at: 1391 2025 2072 2344 2804 3173 1391 Size: 634 47 272 460 369 4072 Fragments arranged by size: 4072 634 460 369 272 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 928 Size: 971 456 4427 Fragments arranged by size: 4427 971 456 BbvCI CC'TCA_GC Cuts at: 2321 2321 Size: 5854 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4045 4079 1903 Size: 34 2108 34 3678 Fragments arranged by size: 3678 2108 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3746 4956 3746 Size: 1210 4644 BclI T'GATC_A Cuts at: 1548 1548 Size: 5854 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4465 136 Size: 107 122 71 631 605 2793 1525 Fragments arranged by size: 2793 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5849 5849 Size: 5854 BlpI GC'TnA_GC Cuts at: 2811 3271 2811 Size: 460 5394 Bme1580I G_kGCm'C Cuts at: 1790 2083 2344 2694 3033 3915 5071 1790 Size: 293 261 350 339 882 1156 2573 Fragments arranged by size: 2573 1156 882 350 339 293 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 2200 3585 Size: 416 53 83 399 24 708 75 1385 Cuts at: 3585 4543 442 Size: 958 1753 Fragments arranged by size: 1753 1385 958 708 416 399 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4514 1239 Size: 3275 2579 Bpu10I CC'TnA_GC Cuts at: 2321 2321 Size: 5854 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3979 4844 5142 3979 Size: 865 298 4691 Fragments arranged by size: 4691 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2897 4517 882 Size: 617 1398 1620 2219 Fragments arranged by size: 2219 1620 1398 617 BsaAI yAC'GTr Cuts at: 493 1204 3645 493 Size: 711 2441 2702 Fragments arranged by size: 2702 2441 711 BsaBI GATnn'nnATC Cuts at: 5848 5848 Size: 5854 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2892 3661 4043 4678 Size: 53 83 186 936 1359 769 382 635 Cuts at: 4678 275 Size: 1451 Fragments arranged by size: 1451 1359 936 769 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4287 4685 4959 5106 5517 4287 Size: 398 274 147 411 4624 Fragments arranged by size: 4624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5629 Fragments arranged by size: 5629 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 3081 1186 Size: 829 1066 3959 Fragments arranged by size: 3959 1066 829 BseYI C'CCAG_C Cuts at: 2937 5057 5553 2937 Size: 2120 496 3238 Fragments arranged by size: 3238 2120 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2467 1163 Size: 573 731 4550 Fragments arranged by size: 4550 731 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3304 4065 5093 1540 Size: 275 186 1303 761 1028 2301 Fragments arranged by size: 2301 1303 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3915 4000 5071 3915 Size: 85 1071 4698 Fragments arranged by size: 4698 1071 85 BsmI GAATG_Cn' Cuts at: 3365 3458 3365 Size: 93 5761 BsmBI CGTCTCn'nnnn_ Cuts at: 2291 2291 Size: 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2072 2083 2344 2694 2804 3033 Size: 399 235 47 11 261 350 110 229 Cuts at: 3033 3173 3915 4000 5071 1391 Size: 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 261 235 229 140 110 85 47 11 BspEI T'CCGG_A Cuts at: 5517 5517 Size: 5854 BspHI T'CATG_A Cuts at: 3743 5473 3743 Size: 1730 4124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2293 2400 3209 844 Size: 1449 107 809 3489 Fragments arranged by size: 3489 1449 809 107 BsrBI CCG'CTC Cuts at: 1394 1823 3741 1394 Size: 429 1918 3507 Fragments arranged by size: 3507 1918 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2719 2933 4349 4523 65 Size: 1183 1471 214 1416 174 1396 Fragments arranged by size: 1471 1416 1396 1183 214 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2486 4498 4685 1700 Size: 110 676 2012 187 2869 Fragments arranged by size: 2869 2012 676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5854 BssSI C'ACGA_G Cuts at: 3914 4926 3914 Size: 1012 4842 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5854 BstBI TT'CG_AA Cuts at: 4658 4658 Size: 5854 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5854 BstYI r'GATC_y Cuts at: 1972 2667 3184 3551 3936 3953 5405 5491 5503 Size: 695 517 367 385 17 1452 86 12 Cuts at: 5503 5849 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 695 517 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2475 513 Size: 552 939 471 3892 Fragments arranged by size: 3892 939 552 471 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2946 487 Size: 683 56 1720 3395 Fragments arranged by size: 3395 1720 683 56 BtsI GCAGTG_nn' Cuts at: 2225 2527 3370 4164 4184 2225 Size: 302 843 794 20 3895 Fragments arranged by size: 3895 843 794 302 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5854 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5819 DraI TTT'AAA Cuts at: 2602 3158 2602 Size: 556 5298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2208 4861 809 Size: 507 892 2653 1802 Fragments arranged by size: 2653 1802 892 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3301 4190 5819 Size: 54 1425 50 275 18 1471 889 1629 Cuts at: 5819 8 Size: 43 Fragments arranged by size: 1629 1471 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3301 1537 Size: 275 1489 4090 Fragments arranged by size: 4090 1489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2042 2235 2975 3784 1353 Size: 680 9 193 740 809 3423 Fragments arranged by size: 3423 809 740 680 193 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4441 4815 4961 387 Size: 1064 138 2852 374 146 1280 Fragments arranged by size: 2852 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 2619 3917 4514 5301 5569 1239 Size: 393 717 270 1298 597 787 268 1524 Fragments arranged by size: 1524 1298 787 717 597 393 270 268 EcoO109I rG'GnC_Cy Cuts at: 2340 2340 Size: 5854 EcoRI G'AATT_C Cuts at: 2832 3163 3541 2832 Size: 331 378 5145 Fragments arranged by size: 5145 378 331 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5854 FspI TGC'GCA Cuts at: 4360 4360 Size: 5854 HaeII r_GCGC'y Cuts at: 1678 5001 1678 Size: 3323 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 4495 4527 4569 Size: 32 135 32 773 32 2118 32 42 Cuts at: 4569 4601 1373 Size: 32 2626 Fragments arranged by size: 2626 2118 773 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3198 3444 669 Size: 988 281 1260 246 3079 Fragments arranged by size: 3079 1260 988 281 246 HindIII A'AGCT_T Cuts at: 748 2570 748 Size: 1822 4032 HpaI GTT'AAC Cuts at: 3444 3444 Size: 5854 KpnI G_GTAC'C Cuts at: 3179 3179 Size: 5854 MfeI C'AATT_G Cuts at: 3453 3453 Size: 5854 MluI A'CGCG_T Cuts at: 5730 5730 Size: 5854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2681 4968 5152 1305 Size: 1376 2287 184 2007 Fragments arranged by size: 2287 2007 1376 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4032 Fragments arranged by size: 4032 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2687 2729 3812 4171 4330 518 Size: 648 1329 192 42 1083 359 159 2042 Fragments arranged by size: 2042 1329 1083 648 359 192 159 42 MspA1I CmG'CkG Cuts at: 1606 2522 2818 2952 3270 3947 5095 5340 1606 Size: 916 296 134 318 677 1148 245 2120 Fragments arranged by size: 2120 1148 916 677 318 296 245 134 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4363 Fragments arranged by size: 4363 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5854 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5854 NotI GC'GGCC_GC Cuts at: 3301 3301 Size: 5854 NspI r_CATG'y Cuts at: 1132 1229 2513 3212 4757 1132 Size: 97 1284 699 1545 2229 Fragments arranged by size: 2229 1545 1284 699 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5854 PciI A'CATG_T Cuts at: 4753 4753 Size: 5854 PflMI CCAn_nnn'nTGG Cuts at: 1108 2162 2700 3040 1108 Size: 1054 538 340 3922 Fragments arranged by size: 3922 1054 538 340 PfoI T'CCnGG_A Cuts at: 2751 2751 Size: 5854 PmeI GTTT'AAAC Cuts at: 3158 3158 Size: 5854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3923 3955 5436 5468 3923 Size: 32 1481 32 4309 Fragments arranged by size: 4309 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5854 PsiI TTA'TAA Cuts at: 3424 3424 Size: 5854 PspOMI G'GGCC_C Cuts at: 2340 2340 Size: 5854 PstI C_TGCA'G Cuts at: 830 3206 830 Size: 2376 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5854 PvuII CAG'CTG Cuts at: 2522 2818 2522 Size: 296 5558 SalI G'TCGA_C Cuts at: 1936 3196 1936 Size: 1260 4594 SbfI CC_TGCA'GG Cuts at: 3206 3206 Size: 5854 ScaI AGT'ACT Cuts at: 4102 5814 4102 Size: 1712 4142 SfcI C'TryA_G Cuts at: 826 1045 3202 4337 5018 5209 826 Size: 219 2157 1135 681 191 1471 Fragments arranged by size: 2157 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5854 SmaI CCC'GGG Cuts at: 1872 3181 1872 Size: 1309 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3958 4859 5121 820 Size: 197 926 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3645 493 Size: 3152 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5854 SphI G_CATG'C Cuts at: 1132 3212 1132 Size: 2080 3774 SspI AAT'ATT Cuts at: 5 52 3778 5 Size: 47 3726 2081 Fragments arranged by size: 3726 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5854 StyI C'CwwG_G Cuts at: 513 1065 2004 2694 3293 513 Size: 552 939 690 599 3074 Fragments arranged by size: 3074 939 690 599 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2699 2874 3028 3881 3898 4051 4236 Size: 1255 1119 175 154 853 17 153 185 Cuts at: 4236 4681 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1119 853 445 185 175 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4100 5812 96 Size: 274 80 33 51 1443 2123 1712 138 Fragments arranged by size: 2123 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5814 5846 5814 Size: 32 5822 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5854 XbaI T'CTAG_A Cuts at: 3190 3190 Size: 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5854 XmaI C'CCGG_G Cuts at: 1870 3179 1870 Size: 1309 4545 XmnI GAAnn'nnTTC Cuts at: 2432 3983 2432 Size: 1551 4303 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2893 3662 276 Size: 53 83 186 936 1359 769 2468 Fragments arranged by size: 2468 1359 936 769 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI