Restriction map for
pFN21AE0426
(Linear) (Six-base) MAPSORT of: KIEE0426.seq Check: 1022 from: 1 to: 1149
KIEE0426.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 1149
Size: 288 861
AatII G_ACGT'C
Cuts at: 0 890 1149
Size: 890 259
AccI GT'mk_AC
Cuts at: 0 130 1149
Size: 130 1019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 614 1149
Size: 344 270 535
Fragments arranged by size:
535 344 270
AhdI GACnn_n'nnGTC
Cuts at: 0 354 1149
Size: 354 795
AleI CACnn'nnGTG
Cuts at: 0 490 682 1149
Size: 490 192 467
Fragments arranged by size:
490 467 192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 700 944 1149
Size: 700 244 205
Fragments arranged by size:
700 244 205
ApaI G_GGCC'C
Cuts at: 0 339 1149
Size: 339 810
ApoI r'AATT_y
Cuts at: 0 652 775 827 1149
Size: 652 123 52 322
Fragments arranged by size:
652 322 123 52
AvaI C'yCGr_G
Cuts at: 0 640 1149
Size: 640 509
BamHI G'GATC_C
Cuts at: 0 662 1149
Size: 662 487
BanI G'GyrC_C
Cuts at: 0 484 1025 1149
Size: 484 541 124
Fragments arranged by size:
541 484 124
BanII G_rGCy'C
Cuts at: 0 20 67 339 799 1149
Size: 20 47 272 460 350
Fragments arranged by size:
460 350 272 47 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 1149
Size: 350 799
BbvCI CC'TCA_GC
Cuts at: 0 316 1149
Size: 316 833
BlpI GC'TnA_GC
Cuts at: 0 806 1149
Size: 806 343
Bme1580I G_kGCm'C
Cuts at: 0 78 339 689 1028 1149
Size: 78 261 350 339 121
Fragments arranged by size:
350 339 261 121 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 195 1149
Size: 120 75 954
Fragments arranged by size:
954 120 75
Bpu10I CC'TnA_GC
Cuts at: 0 316 1149
Size: 316 833
BsaI GGTCTCn'nnnn_
Cuts at: 0 892 1149
Size: 892 257
BsaHI Gr'CG_yC
Cuts at: 0 887 1149
Size: 887 262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 1076 1149
Size: 10 1066 73
Fragments arranged by size:
1066 73 10
BseYI C'CCAG_C
Cuts at: 0 932 1149
Size: 932 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1149
Size: 462 687
BsmBI CGTCTCn'nnnn_
Cuts at: 0 286 1149
Size: 286 863
Bsp1286I G_dGCh'C
Cuts at: 0 20 67 78 339 689 799 1028 1149
Size: 20 47 11 261 350 110 229 121
Fragments arranged by size:
350 261 229 121 110 47 20 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 395 1149
Size: 288 107 754
Fragments arranged by size:
754 288 107
BsrDI GCAATG_nn'
Cuts at: 0 714 928 1149
Size: 714 214 221
Fragments arranged by size:
714 221 214
BsrFI r'CCGG_y
Cuts at: 0 481 1149
Size: 481 668
BstYI r'GATC_y
Cuts at: 0 662 1149
Size: 662 487
BtgI C'CryG_G
Cuts at: 0 470 1149
Size: 470 679
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 941 1149
Size: 941 208
BtsI GCAGTG_nn'
Cuts at: 0 220 522 1149
Size: 220 302 627
Fragments arranged by size:
627 302 220
DraI TTT'AAA
Cuts at: 0 597 1149
Size: 597 552
DrdI GACnn_nn'nnGTC
Cuts at: 0 203 1149
Size: 203 946
EarI CTCTTCn'nnn_
Cuts at: 0 28 37 230 970 1149
Size: 28 9 193 740 179
Fragments arranged by size:
740 193 179 28 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 614 1149
Size: 344 270 535
Fragments arranged by size:
535 344 270
EcoO109I rG'GnC_Cy
Cuts at: 0 335 1149
Size: 335 814
EcoRI G'AATT_C
Cuts at: 0 827 1149
Size: 827 322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 1149
Size: 171 978
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 1149
Size: 340 32 777
Fragments arranged by size:
777 340 32
HindIII A'AGCT_T
Cuts at: 0 565 1149
Size: 565 584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1149
Size: 676 473
MslI CAynn'nnrTG
Cuts at: 0 490 682 724 1149
Size: 490 192 42 425
Fragments arranged by size:
490 425 192 42
MspA1I CmG'CkG
Cuts at: 0 517 813 947 1149
Size: 517 296 134 202
Fragments arranged by size:
517 296 202 134
NspI r_CATG'y
Cuts at: 0 508 1149
Size: 508 641
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 695 1035 1149
Size: 157 538 340 114
Fragments arranged by size:
538 340 157 114
PfoI T'CCnGG_A
Cuts at: 0 746 1149
Size: 746 403
PspOMI G'GGCC_C
Cuts at: 0 335 1149
Size: 335 814
PvuII CAG'CTG
Cuts at: 0 517 813 1149
Size: 517 296 336
Fragments arranged by size:
517 336 296
StyI C'CwwG_G
Cuts at: 0 689 1149
Size: 689 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 869 1023 1149
Size: 694 175 154 126
Fragments arranged by size:
694 175 154 126
XmnI GAAnn'nnTTC
Cuts at: 0 427 1149
Size: 427 722
ZraI GAC'GTC
Cuts at: 0 888 1149
Size: 888 261
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI ApaI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BlpI
Bme1580I BmrI Bpu10I BsaI BsaHI BseRI BseYI BsgI
BsmBI Bsp1286I BspMI BsrDI BsrFI BstYI BtgI BtgZI
BtsI DraI DrdI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I HindIII MmeI MslI MspA1I NspI PflMI PfoI
PspOMI PvuII StyI TaqII XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AloI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
BpmI BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EaeI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI