Restriction map for
pFN21AB6135
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AgeI Alw44I AlwNI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BanI BbeI BbvCI BciVI BclI
BfaI BglI BlnI BlpI Bpu1102I
BsaI BsiWI BsmBI BsmI Bsp1407I
BspHI BsrDI BsrGI BssHII Bst1107I
BstZ17I CelII CpoI DraIII DrdI
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MfeI MluNI
MscI MunI NarI NcoI NheI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshBI PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 583 GTAGAC GT^[AC][GT]AC
AccIII 1424 TCCGGA T^CCGGA
AclI 1141 AACGTT AA^CGTT
AcsI 827 AAATTT [GA]^AATT[CT]
AflII 691 CTTAAG C^TTAAG
AocI 1045 CCTGAGG CC^T[ACGT]AGG
ApaI 1206 GGGCCC GGGCC^C
ApoI 827 AAATTT [GA]^AATT[CT]
AvaI 288 CCCGAG C^[CT]CG[GA]G
BamHI 1427 GGATCC G^GATCC
BbrPI 904 CACGTG CAC^GTG
BbsI 328 GAAGAC GAAGAC(2/6)
BcgI 1686 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 691 CTTAAG C^TTAAG
BglII 1076 AGATCT A^GATCT
BpuAI 328 GAAGAC GAAGAC(2/6)
BsaAI 904 CACGTG [CT]AC^GT[GA]
BsaBI 1077 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1424 TCCGGA [AT]^CCGG[AT]
BseAI 1424 TCCGGA T^CCGGA
BsoBI 288 CCCGAG C^[CT]CG[GA]G
BspDI 703 ATCGAT AT^CGAT
BspEI 1424 TCCGGA T^CCGGA
BspLU11I 137 ACATGT A^CATGT
BspMI 535 ACCTGC ACCTGC(4/8)
BssSI 1102 CACGAG CACGAG(-5/-1)
BstBI 1723 TTCGAA TT^CGAA
BstEII 193 GGTGACC G^GT[ACGT]ACC
BstPI 193 GGTGACC G^GT[ACGT]ACC
BstXI 836 CCATGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1045 CCTGAGG CC^T[ACGT]AGG
ClaI 703 ATCGAT AT^CGAT
DsaI 516 CCACGG C^C[GA][CT]GG
Eco81I 1045 CCTGAGG CC^T[ACGT]AGG
EcoO65I 193 GGTGACC G^GT[ACGT]ACC
EcoT14I 791 CCAAGG C^C[AT][AT]GG
HincII 997 GTCAAC GT[CT]^[GA]AC
HindII 997 GTCAAC GT[CT]^[GA]AC
MamI 1077 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1544 ACGCGT A^CGCGT
MroI 1424 TCCGGA T^CCGGA
NaeI 1508 GCCGGC GCC^GGC
NdeI 547 CATATG CA^TATG
NgoMIV 1508 GCCGGC G^CCGGC
NotI 38 GCGGCCGC GC^GGCCGC
NruI 461 TCGCGA TCG^CGA
NspV 1723 TTCGAA TT^CGAA
PmaCI 904 CACGTG CAC^GTG
PmlI 904 CACGTG CAC^GTG
Psp1406I 1141 AACGTT AA^CGTT
PvuII 1672 CAGCTG CAG^CTG
SacI 115 GAGCTC GAGCT^C
SbfI 536 CCTGCAGG CCTGCA^GG
SfuI 1723 TTCGAA TT^CGAA
Sse8387I 536 CCTGCAGG CCTGCA^GG
StyI 791 CCAAGG C^C[AT][AT]GG
XcmI 791 CCAAGGTTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 94 GACGCC G[GA]^CG[CT]C
601 GACGCC
AhdI 963 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1531 GACATCAAGTC
Aor51HI 407 AGCGCT AGC^GCT
509 AGCGCT
AspEI 963 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1531 GACATCAAGTC
BsaHI 94 GACGCC G[GA]^CG[CT]C
601 GACGCC
BsiEI 39 CGGCCG CG[GA][CT]^CG
1070 CGGCCG
BsmAI 134 GAGAC GTCTC(1/5)
622 GAGAC
BsrBI 163 CCGCTC CCGCTC(-3/-3)
33 GAGCGG
BsrFI 13 ACCGGC [GA]^CCGG[CT]
1508 GCCGGC
BstAPI 482 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1226 GCACTTTCTGC
BstYI 1076 AGATCT [GA]^GATC[CT]
1427 GGATCC
Cfr10I 13 ACCGGC [GA]^CCGG[CT]
1508 GCCGGC
DraI 1464 TTTAAA TTT^AAA
1476 TTTAAA
EagI 39 CGGCCG C^GGCCG
1070 CGGCCG
Eam1105I 963 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1531 GACATCAAGTC
EarI 394 CTCTTC CTCTTC(1/4)
229 GAAGAG
EclXI 39 CGGCCG C^GGCCG
1070 CGGCCG
Eco47III 407 AGCGCT AGC^GCT
509 AGCGCT
Eco52I 39 CGGCCG C^GGCCG
1070 CGGCCG
Hin1I 94 GACGCC G[GA]^CG[CT]C
601 GACGCC
Ksp632I 394 CTCTTC CTCTTC(1/4)
229 GAAGAG
MaeIII 194 GTGAC ^GT[ACGT]AC
1214 GTGAC
MflI 1076 AGATCT [GA]^GATC[CT]
1427 GGATCC
MslI 84 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
837 CATGAAAGTG
MspA1I 529 CCGCTG C[AC]G^C[GT]G
1672 CAGCTG
PflMI 356 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1513 CCACAGACTGG
PshAI 136 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1624 GACTACGGTC
PstI 537 CTGCAG CTGCA^G
745 CTGCAG
StuI 267 AGGCCT AGG^CCT
1200 AGGCCT
TfiI 553 GATTC G^A[AT]TC
719 GAATC
Tsp45I 194 GTGAC ^GT[GC]AC
1214 GTGAC
TspRI 253 CAGTG CA[GC]TG[ACGT][ACGT]^
436 CAGTG
Van91I 356 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1513 CCACAGACTGG
XhoII 1076 AGATCT [GA]^GATC[CT]
1427 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI Tru9I TseI Tsp509I
TthHB8I