Restriction map for pFN21AB7519
 (Circular) (Six-base) MAPSORT of: pFN21AB7519.seq  Check: 4968  from: 1  to: 5131



pFN21AB7519  5131 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:45  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2941     278 
   Size:         53      83     186     936    1405    2468

  Fragments arranged by size: 

                2468    1405     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2474    1937 
   Size:        537    4594
 
Acc65I G'GTAC_C

Cuts at:   2452    2452 
   Size:       5131
 
AclI AA'CG_TT

Cuts at:   1563    3258    3631    1563 
   Size:       1695     373    3063

  Fragments arranged by size: 

                3063    1695     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3194    4578    4846    1632 
   Size:       1562    1384     268    1917

  Fragments arranged by size: 

                1917    1562    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4934
 
AflIII A'CryG_T

Cuts at:   1203    4030    5007    1203 
   Size:       2827     977    1327

  Fragments arranged by size: 

                2827    1327     977
 
AgeI A'CCGG_T

Cuts at:   3962    3962 
   Size:       5131
 
AhdI GACnn_n'nnGTC

Cuts at:   3860    3860 
   Size:       5131
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5131
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5131
 
AlwNI CAG_nnn'CTG

Cuts at:   2324    2874    4446    2324 
   Size:        550    1572    3009

  Fragments arranged by size: 

                3009    1572     550
 
ApaLI G'TGCA_C

Cuts at:   3188    4344    3188 
   Size:       1156    3975
 
ApoI r'AATT_y

Cuts at:   1493    2104    2440    2666    2818    4927    1493 
   Size:        611     336     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     611     336     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3685     160 
   Size:        872    2653    1606

  Fragments arranged by size: 

                2653    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5131
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2456    1870 
   Size:         73     513    4545

  Fragments arranged by size: 

                4545     513      73
 
BamHI G'GATC_C

Cuts at:   2461    2828    2461 
   Size:        367    4764
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2452    3907     618 
   Size:        325     217     627     665    1455    1842

  Fragments arranged by size: 

                1842    1455     665     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2175    2450    1391 
   Size:        784     275    4072

  Fragments arranged by size: 

                4072     784     275
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2176     928 
   Size:        971     277    3883

  Fragments arranged by size: 

                3883     971     277
 
BbvCI CC'TCA_GC

Cuts at:   2328    2328 
   Size:       5131
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3322    3356    1903 
   Size:         34    1385      34    3678

  Fragments arranged by size: 

                3678    1385      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3023    4233    3023 
   Size:       1210    3921
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5131
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2303    3742 
   Size:        107     122      71     631     605     337     294    1439

Cuts at:   3742     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1439     631     605     337     294     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5126    5126 
   Size:       5131
 
BlpI GC'TnA_GC

Cuts at:   2548    2548 
   Size:       5131
 
Bme1580I G_kGCm'C

Cuts at:   1790    3192    4348    1790 
   Size:       1402    1156    2573

  Fragments arranged by size: 

                2573    1402    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5131
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2862    3820     442 
   Size:        416      53      83     399      24    1445     958    1753

  Fragments arranged by size: 

                1753    1445     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5131
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2071    2071 
   Size:       5131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2252    3791    1239 
   Size:       1013    1539    2579

  Fragments arranged by size: 

                2579    1539    1013
 
Bpu10I CC'TnA_GC

Cuts at:   2328    2328 
   Size:       5131
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3256    4121    4419    3256 
   Size:        865     298    3968

  Fragments arranged by size: 

                3968     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2231    3794     882 
   Size:        617     732    1563    2219

  Fragments arranged by size: 

                2219    1563     732     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2922     493 
   Size:        711    1718    2702

  Fragments arranged by size: 

                2702    1718     711
 
BsaBI GATnn'nnATC

Cuts at:   5125    5125 
   Size:       5131
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2052    2938    3320    3955 
   Size:         53      83     186     936     519     886     382     635

Cuts at:   3955     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     886     635     519     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3564    3962    4236    4383    4794    3564 
   Size:        398     274     147     411    3901

  Fragments arranged by size: 

                3901     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2351    2381    1178 
   Size:         30     165      30     948      30    3928

  Fragments arranged by size: 

                3928     948     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2060    2345    2393    1186 
   Size:        874     285      48    3924

  Fragments arranged by size: 

                3924     874     285      48
 
BseYI C'CCAG_C

Cuts at:   4334    4830    4334 
   Size:        496    4635
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2175    1163 
   Size:        573     439    4119

  Fragments arranged by size: 

                4119     573     439
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2581    3342    4370    1540 
   Size:        275     186     580     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     580     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2175    3192    3277    4348    2175 
   Size:       1017      85    1071    2958

  Fragments arranged by size: 

                2958    1071    1017      85
 
BsmI GAATG_Cn'

Cuts at:   2642    2735    2642 
   Size:         93    5038
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2175    2450    3192    3277    4348    1391 
   Size:        399     385     275     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     385     275      85
 
BspEI T'CCGG_A

Cuts at:   4794    4794 
   Size:       5131
 
BspHI T'CATG_A

Cuts at:   2187    3020    4750    2187 
   Size:        833    1730    2568

  Fragments arranged by size: 

                2568    1730     833
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2486     844 
   Size:       1642    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3018    1394 
   Size:        429    1195    3507

  Fragments arranged by size: 

                3507    1195     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2241    3626    3800      65 
   Size:       1183     993    1385     174    1396

  Fragments arranged by size: 

                1396    1385    1183     993     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3775    3962    1700 
   Size:        110    1965     187    2869

  Fragments arranged by size: 

                2869    1965     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5131
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5131
 
BssSI C'ACGA_G

Cuts at:   3191    4203    3191 
   Size:       1012    4119
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5131
 
BstBI TT'CG_AA

Cuts at:   3935    3935 
   Size:       5131
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5131
 
BstYI r'GATC_y

Cuts at:   1972    2461    2828    3213    3230    4682    4768    4780    5126 
   Size:        489     367     385      17    1452      86      12     346

Cuts at:   5126    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     489     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2027     513 
   Size:        552     939      23    3617

  Fragments arranged by size: 

                3617     939     552      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4392

  Fragments arranged by size: 

                4392     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2647    3441    3461    2647 
   Size:        794      20    4317

  Fragments arranged by size: 

                4317     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5131
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5096
 
DraI TTT'AAA

Cuts at:   2435    2435 
   Size:       5131
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4138     809 
   Size:        507    2822    1802

  Fragments arranged by size: 

                2822    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2304    2578    3467 
   Size:         54    1425      50     275      18     474     274     889

Cuts at:   3467    5096       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     474     275     274      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2578    1537 
   Size:        275     766    4090

  Fragments arranged by size: 

                4090     766     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3061    1353 
   Size:       1708    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3718    4092    4238     387 
   Size:       1064     138    2129     374     146    1280

  Fragments arranged by size: 

                2129    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2173    2173 
   Size:       5131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2252    3194    3791    4578    4846    1239 
   Size:        393     620     942     597     787     268    1524

  Fragments arranged by size: 

                1524     942     787     620     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2440    2818    2440 
   Size:        378    4753
 
EcoRV GAT'ATC

Cuts at:   2404    2404 
   Size:       5131
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2437    2437 
   Size:       5131
 
FspI TGC'GCA

Cuts at:   3637    3637 
   Size:       5131
 
HaeII r_GCGC'y

Cuts at:   1678    4278    1678 
   Size:       2600    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3772    3804    3846    3878    1373 
   Size:         32     135      32    2200      32      42      32    2626

  Fragments arranged by size: 

                2626    2200     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2475    2721     669 
   Size:        988     281     537     246    3079

  Fragments arranged by size: 

                3079     988     537     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2276    2353     748 
   Size:       1528      77    3526

  Fragments arranged by size: 

                3526    1528      77
 
HpaI GTT'AAC

Cuts at:   2721    2721 
   Size:       5131
 
KpnI G_GTAC'C

Cuts at:   2456    2456 
   Size:       5131
 
MfeI C'AATT_G

Cuts at:   2730    2730 
   Size:       5131
 
MluI A'CGCG_T

Cuts at:   5007    5007 
   Size:       5131
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4245    4429    1305 
   Size:       2940     184    2007

  Fragments arranged by size: 

                2940    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2306      10 
   Size:         54    1425     343     474    2835

  Fragments arranged by size: 

                2835    1425     474     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3089    3448    3607     518 
   Size:        648    1923     359     159    2042

  Fragments arranged by size: 

                2042    1923     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2029    2090    2547    3224    4372    4617    1606 
   Size:        423      61     457     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     457     423     245      61
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3640

  Fragments arranged by size: 

                3640     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2118     387 
   Size:       1731    3400
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5131
 
NotI GC'GGCC_GC

Cuts at:   2578    2578 
   Size:       5131
 
NspI r_CATG'y

Cuts at:   1132    1229    2489    4034    1132 
   Size:         97    1260    1545    2229

  Fragments arranged by size: 

                2229    1545    1260      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5131
 
PciI A'CATG_T

Cuts at:   4030    4030 
   Size:       5131
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5131
 
PmeI GTTT'AAAC

Cuts at:   2435    2435 
   Size:       5131
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2157    2189    3200    3232    4713    4745    2157 
   Size:         32    1011      32    1481      32    2543

  Fragments arranged by size: 

                2543    1481    1011      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5131
 
PsiI TTA'TAA

Cuts at:   2701    2701 
   Size:       5131
 
PstI C_TGCA'G

Cuts at:    830    2388    2483     830 
   Size:       1558      95    3478

  Fragments arranged by size: 

                3478    1558      95
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5131
 
PvuII CAG'CTG

Cuts at:   2090    2090 
   Size:       5131
 
SacI G_AGCT'C

Cuts at:   2175    2175 
   Size:       5131
 
SacII CC_GC'GG

Cuts at:   2030    2030 
   Size:       5131
 
SalI G'TCGA_C

Cuts at:   1936    2473    1936 
   Size:        537    4594
 
SbfI CC_TGCA'GG

Cuts at:   2483    2483 
   Size:       5131
 
ScaI AGT'ACT

Cuts at:   3379    5091    3379 
   Size:       1712    3419
 
SfcI C'TryA_G

Cuts at:    826    1045    2384    2479    3614    4295    4486     826 
   Size:        219    1339      95    1135     681     191    1471

  Fragments arranged by size: 

                1471    1339    1135     681     219     191      95
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2303    2303 
   Size:       5131
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5131
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5131
 
SmaI CCC'GGG

Cuts at:   1872    2458    1872 
   Size:        586    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3235    4136    4398     820 
   Size:        197     926    1292     901     262    1553

  Fragments arranged by size: 

                1553    1292     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2922     493 
   Size:       2429    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5131
 
SphI G_CATG'C

Cuts at:   1132    2489    1132 
   Size:       1357    3774
 
SspI AAT'ATT

Cuts at:      5      52    3055       5 
   Size:         47    3003    2081

  Fragments arranged by size: 

                3003    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5131
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2337    2570     513 
   Size:        552     939     333     233    3074

  Fragments arranged by size: 

                3074     939     552     333     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3158    3175    3328    3513    3958     325 
   Size:       1255    1578      17     153     185     445    1498

  Fragments arranged by size: 

                1578    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3377    5089      96 
   Size:        274      80      33      51    1443    1400    1712     138

  Fragments arranged by size: 

                1712    1443    1400     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5091    5123    5091 
   Size:         32    5099
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5131
 
XbaI T'CTAG_A

Cuts at:   2467    2467 
   Size:       5131
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5131
 
XmaI C'CCGG_G

Cuts at:   1870    2456    1870 
   Size:        586    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3260    3260 
   Size:       5131
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2939     276 
   Size:         53      83     186     936    1405    2468

  Fragments arranged by size: 

                2468    1405     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII     SacI    SacII     SalI     SbfI     ScaI     SfcI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI
 EcoO109I     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    RsrII    SanDI     SapI    SexAI     SfoI     SrfI     SwaI     XcmI
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