Restriction map for
pFN21AB5985
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I ApaLI
ApoI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI DraI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KasI
KpnI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I RcaI SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 1222 ACGTGT A^C[GA][CT]GT
AgeI 1382 ACCGGT A^CCGGT
AocI 970 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1208 AGCGCT AGC^GCT
AscI 1368 GGCGCGCC GG^CGCGCC
AspI 1426 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 361 GGCACC G^G[CT][GA]CC
BglI 883 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 397 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 397 GCTGAGC GC^T[ACGT]AGC
BsaBI 657 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 863 GAGACG CGTCTC(1/5)
BsrBI 1041 CCGCTC CCGCTC(-3/-3)
BssHII 1369 GCGCGC G^CGCGC
BssSI 1156 CTCGTG CACGAG(-5/-1)
BstF5I 1491 CATCC GGATG(2/0)
Bsu36I 970 CCTGAGG CC^T[ACGT]AGG
CelII 397 GCTGAGC GC^T[ACGT]AGC
CpoI 125 CGGACCG CG^G[AT]CCG
DraIII 1153 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1319 CCGCGG C^C[GA][CT]GG
EagI 1163 CGGCCG C^GGCCG
EclXI 1163 CGGCCG C^GGCCG
Eco47III 1208 AGCGCT AGC^GCT
Eco52I 1163 CGGCCG C^GGCCG
Eco81I 970 CCTGAGG CC^T[ACGT]AGG
EcoNI 111 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1491 CATCC GGATG(9/13)
HincII 205 GTTAAC GT[CT]^[GA]AC
HindII 205 GTTAAC GT[CT]^[GA]AC
HpaI 205 GTTAAC GTT^AAC
KspI 1319 CCGCGG CCGC^GG
MamI 657 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 206 TTAA T^TAA
NaeI 1000 GCCGGC GCC^GGC
NgoMIV 1000 GCCGGC G^CCGGC
NspI 791 GCATGT [GA]CATG^[CT]
PaeR7I 34 CTCGAG C^TCGAG
PflFI 1426 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1382 ACCGGT A^CCGGT
PstI 805 CTGCAG CTGCA^G
PvuI 927 CGATCG CGAT^CG
RsrII 125 CGGACCG CG^G[AT]CCG
SacI 1009 GAGCTC GAGCT^C
SacII 1319 CCGCGG CCGC^GG
SfiI 882 GGCCAACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 206 TTAA T^TAA
Tth111I 1426 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 34 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 890 GGGCCC GGGCC^C
1073 GGGCCC
Asp700I 909 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1449 GAAGCAGTTC
AspHI 135 GTGCTC G[AT]GC[AT]^C
1009 GAGCTC
BalI 752 TGGCCA TGG^CCA
991 TGGCCA
BbrPI 1177 CACGTG CAC^GTG
1221 CACGTG
BfaI 460 CTAG C^TAG
1195 CTAG
BsaAI 1177 CACGTG [CT]AC^GT[GA]
1221 CACGTG
BsaI 172 GAGACC GGTCTC(1/5)
322 GAGACC
BsiEI 927 CGATCG CG[GA][CT]^CG
1163 CGGCCG
BsiHKAI 135 GTGCTC G[AT]GC[AT]^C
1009 GAGCTC
EcoT14I 701 CCAAGG C^C[AT][AT]GG
948 CCTTGG
MaeI 460 CTAG C^TAG
1195 CTAG
MluNI 752 TGGCCA TGG^CCA
991 TGGCCA
MscI 752 TGGCCA TGG^CCA
991 TGGCCA
MslI 852 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1428 CATGGTCATG
PflMI 566 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
741 CCAGGTGCTGG
PleI 1402 GACTC GAGTC(4/5)
1066 GAGTC
PmaCI 1177 CACGTG CAC^GTG
1221 CACGTG
PmlI 1177 CACGTG CAC^GTG
1221 CACGTG
PvuII 196 CAGCTG CAG^CTG
473 CAGCTG
SfcI 805 CTGCAG C^T[GA][CT]AG
1443 CTACAG
SmaI 18 CCCGGG CCC^GGG
894 CCCGGG
StyI 701 CCAAGG C^C[AT][AT]GG
948 CCTTGG
Van91I 566 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
741 CCAGGTGCTGG
XcmI 140 CCAGAACTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1088 CCAGCCCGGAGGTGG
XmaCI 18 CCCGGG C^CCGGG
894 CCCGGG
XmaI 18 CCCGGG C^CCGGG
894 CCCGGG
XmnI 909 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1449 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII