(Circular) (Six-base) MAPSORT of: pFN21AB5985.seq Check: 6451 from: 1 to: 6268 pFN21AB5985 6268 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3611 1937 Size: 1674 4594 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3263 3501 4331 5715 5983 1632 Size: 1631 238 830 1384 268 1917 Fragments arranged by size: 1917 1631 1384 830 268 238 AfeI AGC'GCT Cuts at: 3215 3215 Size: 6268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 3227 5167 6144 1203 Size: 2024 1940 977 1327 Fragments arranged by size: 2024 1940 1327 977 AgeI A'CCGG_T Cuts at: 3387 5099 3387 Size: 1712 4556 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3494 3526 3494 Size: 32 6236 AlwNI CAG_nnn'CTG Cuts at: 2174 2577 2752 4011 5583 2174 Size: 403 175 1259 1572 2859 Fragments arranged by size: 2859 1572 1259 403 175 ApaI G_GGCC'C Cuts at: 2899 3082 2899 Size: 183 6085 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 3577 3803 3955 6064 1493 Size: 2084 226 152 2109 1697 Fragments arranged by size: 2109 2084 1697 226 152 AscI GG'CGCG_CC Cuts at: 3374 3374 Size: 6268 AseI AT'TA_AT Cuts at: 160 1032 4822 160 Size: 872 3790 1606 Fragments arranged by size: 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2039 2899 3068 3318 3593 1870 Size: 73 80 16 860 169 250 275 4545 Fragments arranged by size: 4545 860 275 250 169 80 73 16 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 3589 5044 618 Size: 325 217 627 579 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 579 325 217 BanII G_rGCy'C Cuts at: 1391 2048 2261 2899 3018 3082 3587 1391 Size: 657 213 638 119 64 505 4072 Fragments arranged by size: 4072 657 638 505 213 119 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4459 4493 1903 Size: 34 2522 34 3678 Fragments arranged by size: 3678 2522 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2894 4879 136 Size: 107 122 71 631 605 1222 1985 1525 Fragments arranged by size: 1985 1525 1222 631 605 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 2403 3685 2403 Size: 1282 4986 Bme1580I G_kGCm'C Cuts at: 1790 2899 3082 3319 4329 5485 1790 Size: 1109 183 237 1010 1156 2573 Fragments arranged by size: 2573 1156 1109 1010 237 183 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2565 2902 3999 Size: 416 53 83 399 24 1148 337 1097 Cuts at: 3999 4957 442 Size: 958 1753 Fragments arranged by size: 1753 1148 1097 958 416 399 337 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2128 2599 3386 3521 4928 1239 Size: 889 471 787 135 1407 2579 Fragments arranged by size: 2579 1407 889 787 471 135 Bpu10I CC'TnA_GC Cuts at: 3049 3086 3049 Size: 37 6231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2136 2975 3134 4393 5258 5556 2136 Size: 839 159 1259 865 298 2848 Fragments arranged by size: 2848 1259 865 839 298 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 2321 4931 882 Size: 617 672 150 2610 2219 Fragments arranged by size: 2610 2219 672 617 150 BsaAI yAC'GTr Cuts at: 493 1204 3184 3228 4059 493 Size: 711 1980 44 831 2702 Fragments arranged by size: 2702 1980 831 711 44 BsaBI GATnn'nnATC Cuts at: 2666 6262 2666 Size: 3596 2672 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2881 3285 3330 4075 Size: 53 83 186 936 1348 404 45 745 Cuts at: 4075 4457 5092 275 Size: 382 635 1451 Fragments arranged by size: 1451 1348 936 745 635 404 382 186 83 53 45 BsaWI w'CCGG_w Cuts at: 2133 2778 3387 4701 5099 5373 5520 5931 2133 Size: 645 609 1314 398 274 147 411 2470 Fragments arranged by size: 2470 1314 645 609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3494 3524 1178 Size: 30 165 30 2091 30 3922 Fragments arranged by size: 3922 2091 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2708 2993 3400 1186 Size: 1522 285 407 4054 Fragments arranged by size: 4054 1522 407 285 BseYI C'CCAG_C Cuts at: 2203 2319 2346 2442 2480 2731 3019 3535 5471 Size: 116 27 96 38 251 288 516 1936 Cuts at: 5471 5967 2203 Size: 496 2504 Fragments arranged by size: 2504 1936 516 496 288 251 116 96 38 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5695 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3171 3718 4479 5507 1540 Size: 275 186 934 236 547 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 547 275 236 186 BsiHKAI G_wGCw'C Cuts at: 2144 3018 4329 4414 5485 2144 Size: 874 1311 85 1071 2927 Fragments arranged by size: 2927 1311 1071 874 85 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 BsmBI CGTCTCn'nnnn_ Cuts at: 2862 2862 Size: 6268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2144 2261 2899 3018 3082 3319 Size: 399 258 96 117 638 119 64 237 Cuts at: 3319 3587 4329 4414 5485 1391 Size: 268 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 638 399 268 258 237 119 117 96 85 64 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 4157 5887 4157 Size: 1730 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3623 844 Size: 2779 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3048 4155 1394 Size: 429 1225 1107 3507 Fragments arranged by size: 3507 1225 1107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4763 4937 65 Size: 1183 3515 174 1396 Fragments arranged by size: 3515 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2785 3005 3387 4912 5099 1700 Size: 110 975 220 382 1525 187 2869 Fragments arranged by size: 2869 1525 975 382 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 3374 1925 Size: 1449 4819 BssSI C'ACGA_G Cuts at: 3161 4328 5340 3161 Size: 1167 1012 4089 Fragments arranged by size: 4089 1167 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6268 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstYI r'GATC_y Cuts at: 1972 2170 2583 3504 3598 3965 4350 4367 5819 Size: 198 413 921 94 367 385 17 1452 Cuts at: 5819 5905 5917 6263 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 921 413 385 367 346 198 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2976 2976 Size: 6268 BtgI C'CryG_G Cuts at: 513 1065 2004 3324 513 Size: 552 939 1320 3457 Fragments arranged by size: 3457 1320 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 2199 3120 3784 4578 4598 2199 Size: 921 664 794 20 3869 Fragments arranged by size: 3869 921 794 664 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DraIII CAC_nnn'GTG Cuts at: 3163 3163 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2757 2886 2996 Size: 54 1425 50 275 18 927 129 110 Cuts at: 2996 3168 3715 4604 6233 8 Size: 172 547 889 1629 43 Fragments arranged by size: 1629 1425 927 889 547 275 172 129 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3168 3715 1537 Size: 275 1356 547 4090 Fragments arranged by size: 4090 1356 547 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2387 3356 4198 1353 Size: 966 68 969 842 3423 Fragments arranged by size: 3423 969 966 842 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2349 2920 4855 5229 5375 387 Size: 1064 138 760 571 1935 374 146 1280 Fragments arranged by size: 1935 1280 1064 760 571 374 146 138 EcoICRI GAG'CTC Cuts at: 3016 3016 Size: 6268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2599 3263 3386 3501 3521 4331 Size: 393 496 471 664 123 115 20 810 Cuts at: 4331 4928 5715 5983 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 810 787 664 597 496 471 393 268 123 115 20 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2120 Size: 6268 EcoO109I rG'GnC_Cy Cuts at: 2020 2111 2229 2737 2896 3053 3078 3079 3466 Size: 91 118 508 159 157 25 1 387 Cuts at: 3466 2020 Size: 4822 Fragments arranged by size: 4822 508 387 159 157 118 91 25 1 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2935 3160 2935 Size: 225 6043 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 2063 3093 3217 5415 1678 Size: 385 1030 124 2198 2531 Fragments arranged by size: 2531 2198 1030 385 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4909 4941 4983 5015 1373 Size: 32 135 32 3337 32 42 32 2626 Fragments arranged by size: 3337 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2212 3612 3858 669 Size: 988 281 274 1400 246 3079 Fragments arranged by size: 3079 1400 988 281 274 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6268 HpaI GTT'AAC Cuts at: 2212 3858 2212 Size: 1646 4622 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 3867 3867 Size: 6268 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 5382 5566 1305 Size: 927 3150 184 2007 Fragments arranged by size: 3150 2007 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2759 2998 10 Size: 54 1425 343 927 239 3280 Fragments arranged by size: 3280 1425 927 343 239 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 3437 4226 4585 4744 518 Size: 648 1695 576 789 359 159 2042 Fragments arranged by size: 2042 1695 789 648 576 359 159 MspA1I CmG'CkG Cuts at: 1606 2203 2480 3326 3342 3684 4361 5509 5754 Size: 597 277 846 16 342 677 1148 245 Cuts at: 5754 1606 Size: 2120 Fragments arranged by size: 2120 1148 846 677 597 342 277 245 16 NaeI GCC'GGC Cuts at: 3007 3007 Size: 6268 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NgoMIV G'CCGG_C Cuts at: 3005 3005 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 2800 3626 5171 1132 Size: 97 1571 826 1545 2229 Fragments arranged by size: 2229 1571 1545 826 97 PasI CC'CwG_GG Cuts at: 1348 2051 2494 3125 1348 Size: 703 443 631 4491 Fragments arranged by size: 4491 703 631 443 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 2577 2752 1108 Size: 1469 175 4624 Fragments arranged by size: 4624 1469 175 PfoI T'CCnGG_A Cuts at: 3498 3498 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PmlI CAC'GTG Cuts at: 3184 3228 3184 Size: 44 6224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 4337 4369 5850 5882 3494 Size: 32 811 32 1481 32 3880 Fragments arranged by size: 3880 1481 811 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 2229 2737 2111 Size: 118 508 5642 Fragments arranged by size: 5642 508 118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PspOMI G'GGCC_C Cuts at: 2895 3078 2895 Size: 183 6085 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3539 3571 3539 Size: 32 6236 PstI C_TGCA'G Cuts at: 830 2814 3620 830 Size: 1984 806 3478 Fragments arranged by size: 3478 1984 806 PvuI CG_AT'CG Cuts at: 2001 2935 2001 Size: 934 5334 PvuII CAG'CTG Cuts at: 2203 2480 2203 Size: 277 5991 RsrII CG'GwC_CG Cuts at: 2131 2131 Size: 6268 SacI G_AGCT'C Cuts at: 3018 3018 Size: 6268 SacII CC_GC'GG Cuts at: 3327 3327 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SanDI GG'GwC_CC Cuts at: 2111 2229 2737 2111 Size: 118 508 5642 Fragments arranged by size: 5642 508 118 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2810 3448 3616 4751 5432 5623 826 Size: 219 1765 638 168 1135 681 191 1471 Fragments arranged by size: 1765 1471 1135 681 638 219 191 168 SfiI GGCCn_nnn'nGGCC Cuts at: 2894 2894 Size: 6268 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 2025 2901 3595 1872 Size: 153 876 694 4545 Fragments arranged by size: 4545 876 694 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 2039 2151 2990 3149 4372 5273 Size: 197 926 96 112 839 159 1223 901 Cuts at: 5273 5535 820 Size: 262 1553 Fragments arranged by size: 1553 1223 926 901 839 262 197 159 112 96 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3626 1132 Size: 2494 3774 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2004 2706 2953 3707 513 Size: 552 939 702 247 754 3074 Fragments arranged by size: 3074 939 754 702 552 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2319 3511 4295 4312 4465 4650 5095 Size: 1255 739 1192 784 17 153 185 445 Cuts at: 5095 325 Size: 1498 Fragments arranged by size: 1498 1255 1192 784 739 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3546 4514 6226 Size: 274 80 33 51 1443 1569 968 1712 Cuts at: 6226 96 Size: 138 Fragments arranged by size: 1712 1569 1443 968 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 6228 6260 2123 Size: 32 4073 32 2131 Fragments arranged by size: 4073 2131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3434 1316 Size: 2118 4150 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2152 3100 2011 Size: 141 948 5179 Fragments arranged by size: 5179 948 141 XhoI C'TCGA_G Cuts at: 1943 2039 1943 Size: 96 6172 XmaI C'CCGG_G Cuts at: 1870 2023 2899 3593 1870 Size: 153 876 694 4545 Fragments arranged by size: 4545 876 694 153 XmnI GAAnn'nnTTC Cuts at: 2918 3458 4397 2918 Size: 540 939 4789 Fragments arranged by size: 4789 939 540 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI SapI SexAI SfoI SrfI SwaI