(Circular) (Six-base) MAPSORT of: pFN21AB6489.seq Check: 1459 from: 1 to: 5692 pFN21AB6489 5692 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2033 2600 2033 Size: 567 5125 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 2017 3013 2017 Size: 996 4696 AclI AA'CG_TT Cuts at: 1563 2983 3819 4192 1563 Size: 1420 836 373 3063 Fragments arranged by size: 3063 1420 836 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2566 3755 5139 5407 1632 Size: 731 203 1189 1384 268 1917 Fragments arranged by size: 1917 1384 1189 731 268 203 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 2371 2408 4591 5568 1203 Size: 1168 37 2183 977 1327 Fragments arranged by size: 2183 1327 1168 977 37 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2030 2780 2870 3435 5007 2030 Size: 750 90 565 1572 2715 Fragments arranged by size: 2715 1572 750 565 90 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 3017 1870 Size: 73 1074 4545 Fragments arranged by size: 4545 1074 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2173 2206 2173 Size: 33 5659 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 3013 4468 618 Size: 325 217 627 230 996 1455 1842 Fragments arranged by size: 1842 1455 996 627 325 230 217 BanII G_rGCy'C Cuts at: 1391 3011 1391 Size: 1620 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2484 928 Size: 971 585 4136 Fragments arranged by size: 4136 971 585 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2174 2246 3584 4794 2174 Size: 72 1338 1210 3072 Fragments arranged by size: 3072 1338 1210 72 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2969 5687 2969 Size: 2718 2974 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 3753 4909 1790 Size: 1963 1156 2573 Fragments arranged by size: 2573 1963 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2196 3423 4381 Size: 416 53 83 399 24 779 1227 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1227 958 779 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 4352 1239 Size: 1412 1701 2579 Fragments arranged by size: 2579 1701 1412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2569 4355 882 Size: 617 1070 1786 2219 Fragments arranged by size: 2219 1786 1070 617 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 2832 4125 4523 4797 4944 5355 2247 Size: 585 1293 398 274 147 411 2584 Fragments arranged by size: 2584 1293 585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 1178 Size: 30 165 30 1221 30 4216 Fragments arranged by size: 4216 1221 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 1186 Size: 896 4796 BseYI C'CCAG_C Cuts at: 4895 5391 4895 Size: 496 5196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 2710 1163 Size: 573 921 53 4145 Fragments arranged by size: 4145 921 573 53 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3753 3838 4909 3753 Size: 85 1071 4536 Fragments arranged by size: 4536 1071 85 BsmI GAATG_Cn' Cuts at: 2506 3203 3296 2506 Size: 697 93 4902 Fragments arranged by size: 4902 697 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3011 3753 3838 4909 1391 Size: 399 1221 742 85 1071 2174 Fragments arranged by size: 2174 1221 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2832 5355 2832 Size: 2523 3169 BspHI T'CATG_A Cuts at: 2562 3581 5311 2562 Size: 1019 1730 2943 Fragments arranged by size: 2943 1730 1019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 2600 3047 844 Size: 1189 567 447 3489 Fragments arranged by size: 3489 1189 567 447 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2865 4187 4361 65 Size: 1183 1617 1322 174 1396 Fragments arranged by size: 1617 1396 1322 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4336 4523 1700 Size: 110 2526 187 2869 Fragments arranged by size: 2869 2526 187 110 BsrGI T'GTAC_A Cuts at: 96 2700 96 Size: 2604 3088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2228 2298 2228 Size: 70 5622 BstYI r'GATC_y Cuts at: 1972 2074 2969 3022 3389 3774 3791 5243 5329 Size: 102 895 53 367 385 17 1452 86 Cuts at: 5329 5341 5687 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 895 385 367 346 102 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 2645 3208 4002 4022 2645 Size: 563 794 20 4315 Fragments arranged by size: 4315 794 563 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657 Size: 54 1425 50 275 18 1309 889 1629 Cuts at: 5657 8 Size: 43 Fragments arranged by size: 1629 1425 1309 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3622 1353 Size: 2269 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4279 4653 4799 387 Size: 1064 138 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2566 2651 3755 4352 5139 5407 Size: 393 731 203 85 1104 597 787 268 Cuts at: 5407 1239 Size: 1524 Fragments arranged by size: 1524 1104 787 731 597 393 268 203 85 EcoO109I rG'GnC_Cy Cuts at: 2123 2520 2123 Size: 397 5295 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2780 2795 2812 2827 4333 Size: 32 135 32 1208 15 17 15 1506 Cuts at: 4333 4365 4407 4439 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1506 1208 135 42 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 2021 3017 2021 Size: 996 4696 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 2838 4806 4990 1305 Size: 1035 498 1968 184 2007 Fragments arranged by size: 2007 1968 1035 498 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 3650 4009 4168 518 Size: 648 1401 1083 359 159 2042 Fragments arranged by size: 2042 1401 1083 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2138 3108 3785 4933 5178 1606 Size: 532 970 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 677 532 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5140 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 2375 2412 3050 4595 1132 Size: 97 1146 37 638 1545 2229 Fragments arranged by size: 2229 1545 1146 638 97 37 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 2371 2408 4591 2371 Size: 37 2183 3472 Fragments arranged by size: 3472 2183 37 PflMI CCAn_nnn'nTGG Cuts at: 1108 2153 2231 1108 Size: 1045 78 4569 Fragments arranged by size: 4569 1045 78 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2123 2123 Size: 5692 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PstI C_TGCA'G Cuts at: 830 2044 3044 830 Size: 1214 1000 3478 Fragments arranged by size: 3478 1214 1000 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2138 2138 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 2044 3044 2044 Size: 1000 4692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2040 2185 3040 4175 4856 5047 826 Size: 219 995 145 855 1135 681 191 1471 Fragments arranged by size: 1471 1135 995 855 681 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 2083 1517 Size: 566 5126 StyI C'CwwG_G Cuts at: 513 1065 2078 3131 513 Size: 552 1013 1053 3074 Fragments arranged by size: 3074 1053 1013 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3719 3736 3889 4074 4519 325 Size: 1255 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 3938 5650 Size: 274 80 33 51 1443 723 1238 1712 Cuts at: 5650 96 Size: 138 Fragments arranged by size: 1712 1443 1238 723 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2624 2656 5652 5684 2624 Size: 32 2996 32 2632 Fragments arranged by size: 2996 2632 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2228 2626 2011 Size: 217 398 5077 Fragments arranged by size: 5077 398 217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI