(Circular) (Six-base) MAPSORT of: pFN21AB6356.seq Check: 9724 from: 1 to: 5590 pFN21AB6356 5590 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2073 2128 2911 2073 Size: 55 783 4752 Fragments arranged by size: 4752 783 55 AclI AA'CG_TT Cuts at: 1563 2291 3717 4090 1563 Size: 728 1426 373 3063 Fragments arranged by size: 3063 1426 728 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 2841 4489 5466 1203 Size: 1638 1648 977 1327 Fragments arranged by size: 1648 1638 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 2350 2490 3333 4905 2350 Size: 140 843 1572 3035 Fragments arranged by size: 3035 1572 843 140 ApaI G_GGCC'C Cuts at: 2103 2634 2874 2103 Size: 531 240 4819 Fragments arranged by size: 4819 531 240 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2234 2899 3125 3277 5386 1493 Size: 741 665 226 152 2109 1697 Fragments arranged by size: 2109 1697 741 665 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2435 2682 2915 1870 Size: 73 159 333 247 233 4545 Fragments arranged by size: 4545 333 247 233 159 73 AvrII C'CTAG_G Cuts at: 2296 2296 Size: 5590 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2110 2128 2218 2399 Size: 325 217 627 286 37 18 90 181 Cuts at: 2399 2534 2911 4366 618 Size: 135 377 1455 1842 Fragments arranged by size: 1842 1455 627 377 325 286 217 181 135 90 37 18 BanII G_rGCy'C Cuts at: 1391 2103 2634 2874 2909 1391 Size: 712 531 240 35 4072 Fragments arranged by size: 4072 712 531 240 35 BbeI G_GCGC'C Cuts at: 2114 2403 2114 Size: 289 5301 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2684 928 Size: 971 785 3834 Fragments arranged by size: 3834 971 785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 2251 1548 Size: 703 4887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2499 4201 136 Size: 107 122 71 631 605 827 1702 1525 Fragments arranged by size: 1702 1525 827 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 2277 3007 2277 Size: 730 4860 Bme1580I G_kGCm'C Cuts at: 1790 2103 2121 2634 2874 3651 4807 1790 Size: 313 18 513 240 777 1156 2573 Fragments arranged by size: 2573 1156 777 513 313 240 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 4279 442 Size: 416 53 83 399 24 1904 958 1753 Fragments arranged by size: 1904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2762 2762 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 4250 1239 Size: 1250 1761 2579 Fragments arranged by size: 2579 1761 1250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3715 4580 4878 3715 Size: 865 298 4427 Fragments arranged by size: 4427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2300 4253 882 Size: 617 801 1953 2219 Fragments arranged by size: 2219 1953 801 617 BsaAI yAC'GTr Cuts at: 493 1204 2551 3381 493 Size: 711 1347 830 2702 Fragments arranged by size: 2702 1347 830 711 BsaBI GATnn'nnATC Cuts at: 2601 5584 2601 Size: 2983 2607 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2400 3397 3779 Size: 53 83 186 936 578 289 997 382 Cuts at: 3779 4414 275 Size: 635 1451 Fragments arranged by size: 1451 997 936 635 578 382 289 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2381 2874 1186 Size: 1195 493 3902 Fragments arranged by size: 3902 1195 493 BseYI C'CCAG_C Cuts at: 2137 2301 2815 2867 4793 5289 2137 Size: 164 514 52 1926 496 2438 Fragments arranged by size: 2438 1926 514 496 164 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 2456 1163 Size: 573 528 192 4297 Fragments arranged by size: 4297 573 528 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2364 2411 2605 3040 3801 4829 Size: 275 186 363 47 194 435 761 1028 Cuts at: 4829 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 435 363 275 194 186 47 BsiHKAI G_wGCw'C Cuts at: 3651 3736 4807 3651 Size: 85 1071 4434 Fragments arranged by size: 4434 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2121 2634 2874 2909 3651 3736 Size: 399 313 18 513 240 35 742 85 Cuts at: 3736 4807 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 513 399 313 240 85 35 18 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 2945 844 Size: 1296 805 3489 Fragments arranged by size: 3489 1296 805 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4085 4259 65 Size: 1183 2837 174 1396 Fragments arranged by size: 2837 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 2406 4234 4421 1700 Size: 110 273 323 1828 187 2869 Fragments arranged by size: 2869 1828 323 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 2379 2490 794 Size: 1585 111 3894 Fragments arranged by size: 3894 1585 111 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 513 Size: 552 939 171 3928 Fragments arranged by size: 3928 939 552 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 2207 2704 2726 3106 3900 3920 2207 Size: 497 22 380 794 20 3877 Fragments arranged by size: 3877 794 497 380 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2408 2427 Size: 54 1425 50 275 18 531 47 19 Cuts at: 2427 2545 3037 3926 5555 8 Size: 118 492 889 1629 43 Fragments arranged by size: 1629 1425 889 531 492 275 118 54 50 47 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2361 2408 3037 1537 Size: 275 549 47 629 4090 Fragments arranged by size: 4090 629 549 275 47 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 3520 1353 Size: 809 1358 3423 Fragments arranged by size: 3423 1358 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2411 2453 4177 4551 4697 387 Size: 1064 138 822 42 1724 374 146 1280 Fragments arranged by size: 1724 1280 1064 822 374 146 138 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 3653 4250 5037 5305 1239 Size: 393 857 1164 597 787 268 1524 Fragments arranged by size: 1524 1164 857 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 5590 EcoO109I rG'GnC_Cy Cuts at: 2630 2631 2728 2871 2630 Size: 1 97 143 5349 Fragments arranged by size: 5349 143 97 1 EcoRI G'AATT_C Cuts at: 2234 2899 3277 2234 Size: 665 378 4547 Fragments arranged by size: 4547 665 378 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2090 2114 2403 2868 4737 1678 Size: 412 24 289 465 1869 2531 Fragments arranged by size: 2531 1869 465 412 289 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2299 2331 2551 2583 4231 Size: 32 135 32 727 32 220 32 1648 Cuts at: 4231 4263 4305 4337 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1648 727 220 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KasI G'GCGC_C Cuts at: 2110 2399 2110 Size: 289 5301 KpnI G_GTAC'C Cuts at: 2077 2132 2915 2077 Size: 55 783 4752 Fragments arranged by size: 4752 783 55 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2547 10 Size: 54 1425 343 715 3053 Fragments arranged by size: 3053 1425 715 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3548 3907 4066 518 Size: 648 2382 359 159 2042 Fragments arranged by size: 2382 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2413 2470 2487 2782 3006 3683 4831 5076 Size: 807 57 17 295 224 677 1148 245 Cuts at: 5076 1606 Size: 2120 Fragments arranged by size: 2120 1148 807 677 295 245 224 57 17 NaeI GCC'GGC Cuts at: 2085 2408 2085 Size: 323 5267 NarI GG'CG_CC Cuts at: 2111 2400 2111 Size: 289 5301 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NgoMIV G'CCGG_C Cuts at: 2083 2406 2083 Size: 323 5267 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2845 2948 4493 1132 Size: 97 1616 103 1545 2229 Fragments arranged by size: 2229 1616 1545 103 97 PasI CC'CwG_GG Cuts at: 1348 2069 2350 1348 Size: 721 281 4588 Fragments arranged by size: 4588 721 281 PciI A'CATG_T Cuts at: 2841 4489 2841 Size: 1648 3942 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 1108 Size: 1404 4186 PfoI T'CCnGG_A Cuts at: 2505 2592 2505 Size: 87 5503 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PmlI CAC'GTG Cuts at: 2551 2551 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2099 2630 2870 2099 Size: 531 240 4819 Fragments arranged by size: 4819 531 240 PstI C_TGCA'G Cuts at: 830 2486 2942 830 Size: 1656 456 3478 Fragments arranged by size: 3478 1656 456 PvuI CG_AT'CG Cuts at: 2001 2605 2001 Size: 604 4986 PvuII CAG'CTG Cuts at: 2487 2487 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2482 2555 2771 2938 4073 4754 4945 Size: 219 1437 73 216 167 1135 681 191 Cuts at: 4945 826 Size: 1471 Fragments arranged by size: 1471 1437 1135 681 219 216 191 167 73 SfoI GGC'GCC Cuts at: 2112 2401 2112 Size: 289 5301 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2104 2437 2917 1872 Size: 232 333 480 4545 Fragments arranged by size: 4545 480 333 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 3694 4595 4857 820 Size: 197 926 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SrfI GCCC'GGGC Cuts at: 2104 2104 Size: 5590 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2296 3029 513 Size: 552 939 177 115 733 3074 Fragments arranged by size: 3074 939 733 552 177 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 3634 3787 3972 4417 325 Size: 1255 2037 17 153 185 445 1498 Fragments arranged by size: 2037 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2518 3836 5548 Size: 274 80 33 51 1443 541 1318 1712 Cuts at: 5548 96 Size: 138 Fragments arranged by size: 1712 1443 1318 541 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2102 2435 2915 1870 Size: 232 333 480 4545 Fragments arranged by size: 4545 480 333 232 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI XcmI