################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AlwNI 316 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 62 GGATCC G^GATCC BbsI 222 GAAGAC GAAGAC(2/6) BcnI 414 CCGGG CC^[GC]GG BfaI 148 CTAG C^TAG BpuAI 222 GAAGAC GAAGAC(2/6) BsaBI 45 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 234 GTGCAG GTGCAG(16/14) BstYI 62 GGATCC [GA]^GATC[CT] CfoI 360 GCGC GCG^C DpnII 63 GATC ^GATC DsaI 176 CCACGG C^C[GA][CT]GG FauI 59 GCGGG CCCGC(4/6) HapII 414 CCGG C^CGG HhaI 360 GCGC GCG^C HinP1I 360 GCGC G^CGC HinfI 45 GATTC G^A[ACGT]TC HpaII 414 CCGG C^CGG HphI 30 GGTGA GGTGA(8/7) MaeI 148 CTAG C^TAG MamI 45 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 63 GATC ^GATC MboII 222 GAAGA GAAGA(8/7) MflI 62 GGATCC [GA]^GATC[CT] MslI 11 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 319 CAGCTG C[AC]G^C[GT]G MspI 414 CCGG C^CGG NciI 414 CCGGG CC^[GC]GG NdeII 63 GATC ^GATC NlaIII 199 CATG CATG^ PflMI 176 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 319 CAGCTG CAG^CTG Sau3AI 63 GATC ^GATC TfiI 45 GATTC G^A[AT]TC Van91I 176 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 62 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AluI 304 AGCT AG^CT 320 AGCT AlwI 63 GATCC GGATC(4/5) 62 GGATC BstF5I 111 CATCC GGATG(2/0) 282 CATCC Cfr13I 125 GGCCC G^G[ACGT]CC 404 GGCCC FokI 111 CATCC GGATG(9/13) 282 CATCC NlaIV 62 GGATCC GG[ACGT]^[ACGT]CC 404 GGCCCC Sau96I 125 GGCCC G^G[ACGT]CC 404 GGCCC SfaNI 172 GATGC GCATC(5/9) 239 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BsrI BssKI BstNI EcoRII Fnu4HI HaeIII ItaI MaeIII MnlI MvaI MwoI ScrFI TseI Tsp45I TspRI