(Linear) (Six-base) MAPSORT of: KIEE3515.seq Check: 2568 from: 1 to: 2904 KIEE3515.orf With 182 enzymes: SgfI * July 3, 2012 18:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2863 2904 Size: 2863 41 AccI GT'mk_AC Cuts at: 0 1040 2904 Size: 1040 1864 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 923 1860 2075 2644 2904 Size: 923 937 215 569 260 Fragments arranged by size: 937 923 569 260 215 AleI CACnn'nnGTG Cuts at: 0 781 2794 2904 Size: 781 2013 110 Fragments arranged by size: 2013 781 110 AlwNI CAG_nnn'CTG Cuts at: 0 1614 2904 Size: 1614 1290 ApaI G_GGCC'C Cuts at: 0 484 1079 2904 Size: 484 595 1825 Fragments arranged by size: 1825 595 484 ApoI r'AATT_y Cuts at: 0 1330 1481 1723 2904 Size: 1330 151 242 1181 Fragments arranged by size: 1330 1181 242 151 AvaI C'yCGr_G Cuts at: 0 34 75 126 357 447 554 2904 Size: 34 41 51 231 90 107 2350 Fragments arranged by size: 2350 231 107 90 51 41 34 BamHI G'GATC_C Cuts at: 0 985 2904 Size: 985 1919 BanI G'GyrC_C Cuts at: 0 109 353 461 2904 Size: 109 244 108 2443 Fragments arranged by size: 2443 244 109 108 BanII G_rGCy'C Cuts at: 0 397 484 730 1068 1079 2904 Size: 397 87 246 338 11 1825 Fragments arranged by size: 1825 397 338 246 87 11 BbeI G_GCGC'C Cuts at: 0 113 357 465 2904 Size: 113 244 108 2439 Fragments arranged by size: 2439 244 113 108 BbsI GAAGACnn'nnnn_ Cuts at: 0 812 836 2904 Size: 812 24 2068 Fragments arranged by size: 2068 812 24 BbvCI CC'TCA_GC Cuts at: 0 26 2904 Size: 26 2878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1848 1882 2904 Size: 1848 34 1022 Fragments arranged by size: 1848 1022 34 BglI GCCn_nnn'nGGC Cuts at: 0 385 503 2904 Size: 385 118 2401 Fragments arranged by size: 2401 385 118 BglII A'GATC_T Cuts at: 0 2103 2229 2904 Size: 2103 126 675 Fragments arranged by size: 2103 675 126 Bme1580I G_kGCm'C Cuts at: 0 484 493 1079 2904 Size: 484 9 586 1825 Fragments arranged by size: 1825 586 484 9 BmgBI CAC'GTC Cuts at: 0 2392 2904 Size: 2392 512 BmrI ACTGGGnnnn_n' Cuts at: 0 493 1146 2904 Size: 493 653 1758 Fragments arranged by size: 1758 653 493 BmtI G_CTAG'C Cuts at: 0 1223 2904 Size: 1223 1681 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 2454 2904 Size: 175 2279 450 Fragments arranged by size: 2279 450 175 Bpu10I CC'TnA_GC Cuts at: 0 26 2904 Size: 26 2878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1029 1210 2904 Size: 1029 181 1694 Fragments arranged by size: 1694 1029 181 BsaI GGTCTCn'nnnn_ Cuts at: 0 601 2291 2904 Size: 601 1690 613 Fragments arranged by size: 1690 613 601 BsaAI yAC'GTr Cuts at: 0 2728 2901 2904 Size: 2728 173 3 Fragments arranged by size: 2728 173 3 BsaBI GATnn'nnATC Cuts at: 0 2372 2904 Size: 2372 532 BsaHI Gr'CG_yC Cuts at: 0 41 110 354 462 2582 2904 Size: 41 69 244 108 2120 322 Fragments arranged by size: 2120 322 244 108 69 41 BsaWI w'CCGG_w Cuts at: 0 2540 2904 Size: 2540 364 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 953 983 2904 Size: 953 30 1921 Fragments arranged by size: 1921 953 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 218 304 307 310 313 316 372 2210 Size: 218 86 3 3 3 3 56 1838 Cuts at: 2210 2452 2643 2904 Size: 242 191 261 Fragments arranged by size: 1838 261 242 218 191 86 56 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 493 889 1335 2640 2679 2744 2904 Size: 493 396 446 1305 39 65 160 Fragments arranged by size: 1305 493 446 396 160 65 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 1193 2891 2904 Size: 40 1153 1698 13 Fragments arranged by size: 1698 1153 40 13 BsiEI CG_ry'CG Cuts at: 0 52 104 188 206 377 389 2904 Size: 52 52 84 18 171 12 2515 Fragments arranged by size: 2515 171 84 52 52 18 12 BsiHKAI G_wGCw'C Cuts at: 0 247 397 730 2904 Size: 247 150 333 2174 Fragments arranged by size: 2174 333 247 150 BsmI GAATG_Cn' Cuts at: 0 1487 2550 2904 Size: 1487 1063 354 Fragments arranged by size: 1487 1063 354 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2330 2523 2904 Size: 2330 193 381 Fragments arranged by size: 2330 381 193 Bsp1286I G_dGCh'C Cuts at: 0 247 397 484 493 730 1068 1079 2904 Size: 247 150 87 9 237 338 11 1825 Fragments arranged by size: 1825 338 247 237 150 87 11 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 947 2863 2904 Size: 947 1916 41 Fragments arranged by size: 1916 947 41 BsrBI CCG'CTC Cuts at: 0 234 452 2904 Size: 234 218 2452 Fragments arranged by size: 2452 234 218 BsrDI GCAATG_nn' Cuts at: 0 1145 2904 Size: 1145 1759 BsrFI r'CCGG_y Cuts at: 0 80 464 2904 Size: 80 384 2440 Fragments arranged by size: 2440 384 80 BsrGI T'GTAC_A Cuts at: 0 1283 2904 Size: 1283 1621 BssHII G'CGCG_C Cuts at: 0 65 404 2904 Size: 65 339 2500 Fragments arranged by size: 2500 339 65 BstAPI GCAn_nnn'nTGC Cuts at: 0 1216 2904 Size: 1216 1688 BstYI r'GATC_y Cuts at: 0 985 1097 2103 2229 2904 Size: 985 112 1006 126 675 Fragments arranged by size: 1006 985 675 126 112 BtgI C'CryG_G Cuts at: 0 104 212 398 432 824 2162 2904 Size: 104 108 186 34 392 1338 742 Fragments arranged by size: 1338 742 392 186 108 104 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 508 2904 Size: 508 2396 DraI TTT'AAA Cuts at: 0 810 1379 2904 Size: 810 569 1525 Fragments arranged by size: 1525 810 569 DrdI GACnn_nn'nnGTC Cuts at: 0 1767 2807 2904 Size: 1767 1040 97 Fragments arranged by size: 1767 1040 97 EaeI y'GGCC_r Cuts at: 0 49 101 185 203 374 386 2663 2904 Size: 49 52 84 18 171 12 2277 241 Fragments arranged by size: 2277 241 171 84 52 49 18 12 EagI C'GGCC_G Cuts at: 0 49 101 185 203 374 386 2904 Size: 49 52 84 18 171 12 2518 Fragments arranged by size: 2518 171 84 52 49 18 12 EarI CTCTTCn'nnn_ Cuts at: 0 772 1463 1723 2504 2620 2726 2904 Size: 772 691 260 781 116 106 178 Fragments arranged by size: 781 772 691 260 178 116 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 338 583 2904 Size: 338 245 2321 Fragments arranged by size: 2321 338 245 EcoICRI GAG'CTC Cuts at: 0 395 728 2904 Size: 395 333 2176 Fragments arranged by size: 2176 395 333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 923 1860 2075 2454 2644 2904 Size: 175 748 937 215 379 190 260 Fragments arranged by size: 937 748 379 260 215 190 175 EcoO109I rG'GnC_Cy Cuts at: 0 150 195 480 513 1076 2361 2904 Size: 150 45 285 33 563 1285 543 Fragments arranged by size: 1285 563 543 285 150 45 33 EcoRI G'AATT_C Cuts at: 0 1330 2904 Size: 1330 1574 EcoRV GAT'ATC Cuts at: 0 1929 2904 Size: 1929 975 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2226 2904 Size: 2226 678 HaeII r_GCGC'y Cuts at: 0 113 357 465 2904 Size: 113 244 108 2439 Fragments arranged by size: 2439 244 113 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1027 1059 2035 2067 2216 2248 2904 Size: 1027 32 976 32 149 32 656 Fragments arranged by size: 1027 976 656 149 32 32 32 HincII GTy'rAC Cuts at: 0 2812 2904 Size: 2812 92 HindIII A'AGCT_T Cuts at: 0 2129 2904 Size: 2129 775 KasI G'GCGC_C Cuts at: 0 109 353 461 2904 Size: 109 244 108 2443 Fragments arranged by size: 2443 244 109 108 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2633 2717 2904 Size: 2633 84 187 Fragments arranged by size: 2633 187 84 MslI CAynn'nnrTG Cuts at: 0 751 781 2252 2794 2904 Size: 751 30 1471 542 110 Fragments arranged by size: 1471 751 542 110 30 MspA1I CmG'CkG Cuts at: 0 147 214 519 1207 2904 Size: 147 67 305 688 1697 Fragments arranged by size: 1697 688 305 147 67 NaeI GCC'GGC Cuts at: 0 82 466 2904 Size: 82 384 2438 Fragments arranged by size: 2438 384 82 NarI GG'CG_CC Cuts at: 0 110 354 462 2904 Size: 110 244 108 2442 Fragments arranged by size: 2442 244 110 108 NcoI C'CATG_G Cuts at: 0 824 2904 Size: 824 2080 NgoMIV G'CCGG_C Cuts at: 0 80 464 2904 Size: 80 384 2440 Fragments arranged by size: 2440 384 80 NheI G'CTAG_C Cuts at: 0 1219 2904 Size: 1219 1685 NotI GC'GGCC_GC Cuts at: 0 49 185 2904 Size: 49 136 2719 Fragments arranged by size: 2719 136 49 PpuMI rG'GwC_Cy Cuts at: 0 195 513 2361 2904 Size: 195 318 1848 543 Fragments arranged by size: 1848 543 318 195 PspOMI G'GGCC_C Cuts at: 0 480 1075 2904 Size: 480 595 1829 Fragments arranged by size: 1829 595 480 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2708 2740 2904 Size: 2708 32 164 Fragments arranged by size: 2708 164 32 PvuII CAG'CTG Cuts at: 0 1207 2904 Size: 1207 1697 SacI G_AGCT'C Cuts at: 0 397 730 2904 Size: 397 333 2174 Fragments arranged by size: 2174 397 333 SacII CC_GC'GG Cuts at: 0 215 2904 Size: 215 2689 SanDI GG'GwC_CC Cuts at: 0 195 513 2904 Size: 195 318 2391 Fragments arranged by size: 2391 318 195 SapI GCTCTTCn'nnn_ Cuts at: 0 772 1723 2904 Size: 772 951 1181 Fragments arranged by size: 1181 951 772 ScaI AGT'ACT Cuts at: 0 1881 2904 Size: 1881 1023 SfcI C'TryA_G Cuts at: 0 1562 1606 2852 2904 Size: 1562 44 1246 52 Fragments arranged by size: 1562 1246 52 44 SfoI GGC'GCC Cuts at: 0 111 355 463 2904 Size: 111 244 108 2441 Fragments arranged by size: 2441 244 111 108 SgrAI Cr'CCGG_yG Cuts at: 0 464 2904 Size: 464 2440 SmaI CCC'GGG Cuts at: 0 36 77 128 359 2904 Size: 36 41 51 231 2545 Fragments arranged by size: 2545 231 51 41 36 SmlI C'TyrA_G Cuts at: 0 1008 1225 2904 Size: 1008 217 1679 Fragments arranged by size: 1679 1008 217 SrfI GCCC'GGGC Cuts at: 0 359 2904 Size: 359 2545 SspI AAT'ATT Cuts at: 0 2273 2904 Size: 2273 631 StyI C'CwwG_G Cuts at: 0 824 2904 Size: 824 2080 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1262 2326 2904 Size: 1262 1064 578 Fragments arranged by size: 1262 1064 578 TatI w'GTAC_w Cuts at: 0 737 872 1283 1879 2904 Size: 737 135 411 596 1025 Fragments arranged by size: 1025 737 596 411 135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 317 334 349 366 752 784 2904 Size: 317 17 15 17 386 32 2120 Fragments arranged by size: 2120 386 317 32 17 17 15 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2776 2904 Size: 2776 128 XmaI C'CCGG_G Cuts at: 0 34 75 126 357 2904 Size: 34 41 51 231 2547 Fragments arranged by size: 2547 231 51 41 34 XmnI GAAnn'nnTTC Cuts at: 0 2487 2513 2904 Size: 2487 26 391 Fragments arranged by size: 2487 391 26 Enzymes that do cut: AarI AccI AcuI AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstYI BtgI BtgZI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI PpuMI PspOMI PsrI PvuII SacI SacII SanDI SapI ScaI SfcI SfoI SgrAI SmaI SmlI SrfI SspI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BlpI BplI BsiWI BspEI BspHI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraIII EcoNI FseI FspI FspAI HpaI KpnI MfeI MluI MscI NdeI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI RsrII SalI SbfI SexAI SfiI SgfI SnaBI SpeI SphI StuI SwaI Tth111I XbaI XhoI ZraI