Restriction map for
pFN21AB6375
(Linear) (Six-base) MAPSORT of: KIBB6375.seq Check: 2752 from: 1 to: 810
KIBB6375.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 197 810
Size: 197 613
AflIII A'CryG_T
Cuts at: 0 402 810
Size: 402 408
ApaI G_GGCC'C
Cuts at: 0 523 810
Size: 523 287
ApoI r'AATT_y
Cuts at: 0 484 810
Size: 484 326
BanII G_rGCy'C
Cuts at: 0 140 240 523 810
Size: 140 100 283 287
Fragments arranged by size:
287 283 140 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 320 479 810
Size: 320 159 331
Fragments arranged by size:
331 320 159
BbvCI CC'TCA_GC
Cuts at: 0 80 810
Size: 80 730
BglII A'GATC_T
Cuts at: 0 179 351 810
Size: 179 172 459
Fragments arranged by size:
459 179 172
BlpI GC'TnA_GC
Cuts at: 0 448 810
Size: 448 362
Bme1580I G_kGCm'C
Cuts at: 0 523 810
Size: 523 287
BmrI ACTGGGnnnn_n'
Cuts at: 0 778 810
Size: 778 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 810
Size: 403 407
Bpu10I CC'TnA_GC
Cuts at: 0 80 810
Size: 80 730
BsaAI yAC'GTr
Cuts at: 0 405 810
Size: 405 405
BsaBI GATnn'nnATC
Cuts at: 0 50 810
Size: 50 760
BsaHI Gr'CG_yC
Cuts at: 0 654 810
Size: 654 156
BseYI C'CCAG_C
Cuts at: 0 477 810
Size: 477 333
BsiHKAI G_wGCw'C
Cuts at: 0 449 810
Size: 449 361
BsmI GAATG_Cn'
Cuts at: 0 224 270 810
Size: 224 46 540
Fragments arranged by size:
540 224 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 739 810
Size: 739 71
Bsp1286I G_dGCh'C
Cuts at: 0 140 240 449 523 810
Size: 140 100 209 74 287
Fragments arranged by size:
287 209 140 100 74
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 810
Size: 197 613
BsrDI GCAATG_nn'
Cuts at: 0 425 810
Size: 425 385
BssSI C'ACGA_G
Cuts at: 0 250 810
Size: 250 560
BstBI TT'CG_AA
Cuts at: 0 216 729 810
Size: 216 513 81
Fragments arranged by size:
513 216 81
BstEII G'GTnAC_C
Cuts at: 0 585 810
Size: 585 225
BstYI r'GATC_y
Cuts at: 0 179 351 810
Size: 179 172 459
Fragments arranged by size:
459 179 172
Bsu36I CC'TnA_GG
Cuts at: 0 389 810
Size: 389 421
BtgI C'CryG_G
Cuts at: 0 716 810
Size: 716 94
DraI TTT'AAA
Cuts at: 0 489 810
Size: 489 321
EarI CTCTTCn'nnn_
Cuts at: 0 737 745 810
Size: 737 8 65
Fragments arranged by size:
737 65 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 810
Size: 403 407
EcoO109I rG'GnC_Cy
Cuts at: 0 123 511 520 609 780 810
Size: 123 388 9 89 171 30
Fragments arranged by size:
388 171 123 89 30 9
HincII GTy'rAC
Cuts at: 0 708 810
Size: 708 102
MfeI C'AATT_G
Cuts at: 0 525 810
Size: 525 285
MslI CAynn'nnrTG
Cuts at: 0 567 810
Size: 567 243
MspA1I CmG'CkG
Cuts at: 0 66 681 759 810
Size: 66 615 78 51
Fragments arranged by size:
615 78 66 51
NcoI C'CATG_G
Cuts at: 0 716 810
Size: 716 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 810
Size: 355 455
PmlI CAC'GTG
Cuts at: 0 405 810
Size: 405 405
PpuMI rG'GwC_Cy
Cuts at: 0 123 511 780 810
Size: 123 388 269 30
Fragments arranged by size:
388 269 123 30
PspOMI G'GGCC_C
Cuts at: 0 519 810
Size: 519 291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 276 308 810
Size: 276 32 502
Fragments arranged by size:
502 276 32
PstI C_TGCA'G
Cuts at: 0 194 283 617 810
Size: 194 89 334 193
Fragments arranged by size:
334 194 193 89
PvuII CAG'CTG
Cuts at: 0 66 681 759 810
Size: 66 615 78 51
Fragments arranged by size:
615 78 66 51
SfcI C'TryA_G
Cuts at: 0 41 190 279 613 810
Size: 41 149 89 334 197
Fragments arranged by size:
334 197 149 89 41
SmlI C'TyrA_G
Cuts at: 0 10 810
Size: 10 800
StyI C'CwwG_G
Cuts at: 0 515 716 810
Size: 515 201 94
Fragments arranged by size:
515 201 94
SwaI ATTT'AAAT
Cuts at: 0 489 810
Size: 489 321
TatI w'GTAC_w
Cuts at: 0 167 302 810
Size: 167 135 508
Fragments arranged by size:
508 167 135
XmnI GAAnn'nnTTC
Cuts at: 0 317 810
Size: 317 493
Enzymes that do cut:
AarI AflIII ApaI ApoI BanII BbsI BbvCI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaHI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BssSI
BstBI BstEII BstYI Bsu36I BtgI DraI EarI Eco57MI
EcoO109I HincII MfeI MslI MspA1I NcoI PflMI PmlI
PpuMI PspOMI PsrI PstI PvuII SfcI SmlI StyI
SwaI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI BmgBI BmtI BplI BpuEI
BsaI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HindIII HpaI KasI KpnI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI