(Circular) (Six-base) MAPSORT of: pFN21AE0535.seq Check: 8980 from: 1 to: 5932 pFN21AE0535 5932 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2590 2590 Size: 5932 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2015 3742 278 Size: 53 83 186 936 479 1727 2468 Fragments arranged by size: 2468 1727 936 479 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 3275 1937 Size: 484 854 4594 Fragments arranged by size: 4594 854 484 Acc65I G'GTAC_C Cuts at: 2241 3253 2241 Size: 1012 4920 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2543 3043 3995 5379 5647 1632 Size: 911 500 952 1384 268 1917 Fragments arranged by size: 1917 1384 952 911 500 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 4831 5808 1203 Size: 3628 977 1327 Fragments arranged by size: 3628 1327 977 AgeI A'CCGG_T Cuts at: 4763 4763 Size: 5932 AhdI GACnn_n'nnGTC Cuts at: 4661 4661 Size: 5932 AleI CACnn'nnGTG Cuts at: 1166 2978 3143 1166 Size: 1812 165 3955 Fragments arranged by size: 3955 1812 165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5932 AlwNI CAG_nnn'CTG Cuts at: 3675 5247 3675 Size: 1572 4360 ApaI G_GGCC'C Cuts at: 2666 2666 Size: 5932 ApaLI G'TGCA_C Cuts at: 2674 3989 5145 2674 Size: 1315 1156 3461 Fragments arranged by size: 3461 1315 1156 ApoI r'AATT_y Cuts at: 1493 3241 3467 3619 5728 1493 Size: 1748 226 152 2109 1697 Fragments arranged by size: 2109 1748 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4486 160 Size: 872 3454 1606 Fragments arranged by size: 3454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2739 3257 1870 Size: 73 796 518 4545 Fragments arranged by size: 4545 796 518 73 BamHI G'GATC_C Cuts at: 2583 3262 3629 2583 Size: 679 367 4886 Fragments arranged by size: 4886 679 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 2241 2835 3253 4708 Size: 325 217 627 235 219 594 418 1455 Cuts at: 4708 618 Size: 1842 Fragments arranged by size: 1842 1455 627 594 418 325 235 219 217 BanII G_rGCy'C Cuts at: 1391 2666 2757 2898 2987 3047 3137 3227 3251 Size: 1275 91 141 89 60 90 90 24 Cuts at: 3251 1391 Size: 4072 Fragments arranged by size: 4072 1275 141 91 90 90 89 60 24 BbeI G_GCGC'C Cuts at: 2839 2839 Size: 5932 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4961 BbvCI CC'TCA_GC Cuts at: 2498 2498 Size: 5932 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4123 4157 1903 Size: 34 2186 34 3678 Fragments arranged by size: 3678 2186 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3824 5034 3824 Size: 1210 4722 BclI T'GATC_A Cuts at: 1548 1548 Size: 5932 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2404 4543 136 Size: 107 122 71 631 605 732 2139 1525 Fragments arranged by size: 2139 1525 732 631 605 122 107 71 BglII A'GATC_T Cuts at: 5927 5927 Size: 5932 BlpI GC'TnA_GC Cuts at: 3349 3349 Size: 5932 Bme1580I G_kGCm'C Cuts at: 1790 2025 2666 2678 3993 5149 1790 Size: 235 641 12 1315 1156 2573 Fragments arranged by size: 2573 1315 1156 641 235 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5932 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2329 3663 4621 Size: 416 53 83 399 24 912 1334 958 Cuts at: 4621 442 Size: 1753 Fragments arranged by size: 1753 1334 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2669 4592 1239 Size: 1058 372 1923 2579 Fragments arranged by size: 2579 1923 1058 372 Bpu10I CC'TnA_GC Cuts at: 2096 2498 2096 Size: 402 5530 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4057 4922 5220 4057 Size: 865 298 4769 Fragments arranged by size: 4769 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4595 882 Size: 617 3096 2219 Fragments arranged by size: 3096 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3723 493 Size: 711 2519 2702 Fragments arranged by size: 2702 2519 711 BsaBI GATnn'nnATC Cuts at: 5926 5926 Size: 5932 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2836 3090 3739 Size: 53 83 186 936 479 824 254 649 Cuts at: 3739 4121 4756 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 824 649 635 479 382 254 186 83 53 BsaWI w'CCGG_w Cuts at: 4365 4763 5037 5184 5595 4365 Size: 398 274 147 411 4702 Fragments arranged by size: 4702 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2969 2999 3119 3149 1178 Size: 30 165 30 1566 30 120 30 3961 Fragments arranged by size: 3961 1566 165 120 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5932 BseYI C'CCAG_C Cuts at: 2284 5135 5631 2284 Size: 2851 496 2585 Fragments arranged by size: 2851 2585 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 2537 2999 1163 Size: 573 291 510 462 4096 Fragments arranged by size: 4096 573 510 462 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2678 2757 2898 3114 3150 3993 4078 5149 2678 Size: 79 141 216 36 843 85 1071 3461 Fragments arranged by size: 3461 1071 843 216 141 85 79 36 BsmI GAATG_Cn' Cuts at: 3443 3536 3443 Size: 93 5839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2666 2678 2757 2898 2987 3047 Size: 399 235 641 12 79 141 89 60 Cuts at: 3047 3114 3137 3150 3227 3251 3993 4078 5149 Size: 67 23 13 77 24 742 85 1071 Cuts at: 5149 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 641 399 235 141 89 85 79 77 67 60 24 23 13 12 BspEI T'CCGG_A Cuts at: 5595 5595 Size: 5932 BspHI T'CATG_A Cuts at: 3821 5551 3821 Size: 1730 4202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 2590 2614 2694 3021 3287 844 Size: 1643 103 24 80 327 266 3489 Fragments arranged by size: 3489 1643 327 266 103 80 24 BsrBI CCG'CTC Cuts at: 1394 1823 2914 3819 1394 Size: 429 1091 905 3507 Fragments arranged by size: 3507 1091 905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4427 4601 65 Size: 1183 3179 174 1396 Fragments arranged by size: 3179 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2244 2832 4576 4763 1700 Size: 110 434 588 1744 187 2869 Fragments arranged by size: 2869 1744 588 434 187 110 BsrGI T'GTAC_A Cuts at: 96 2334 2430 96 Size: 2238 96 3598 Fragments arranged by size: 3598 2238 96 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 3992 5004 3992 Size: 1012 4920 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5932 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 2635 2734 1184 Size: 1451 99 4382 Fragments arranged by size: 4382 1451 99 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2980 2215 Size: 765 5167 BstYI r'GATC_y Cuts at: 1972 2583 3262 3629 4014 4031 5483 5569 5581 Size: 611 679 367 385 17 1452 86 12 Cuts at: 5581 5927 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 679 611 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2057 513 Size: 552 939 12 41 4388 Fragments arranged by size: 4388 939 552 41 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5193 Fragments arranged by size: 5193 683 56 BtsI GCAGTG_nn' Cuts at: 2088 2166 2794 3448 4242 4262 2088 Size: 78 628 654 794 20 3758 Fragments arranged by size: 3758 794 654 628 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5932 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DraIII CAC_nnn'GTG Cuts at: 2560 2560 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4939 809 Size: 507 3623 1802 Fragments arranged by size: 3623 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897 Size: 54 1425 50 275 18 1549 889 1629 Cuts at: 5897 8 Size: 43 Fragments arranged by size: 1629 1549 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2903 3862 1353 Size: 1550 959 3423 Fragments arranged by size: 3423 1550 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2077 4519 4893 5039 387 Size: 1064 138 488 2442 374 146 1280 Fragments arranged by size: 2442 1280 1064 488 374 146 138 EcoICRI GAG'CTC Cuts at: 2755 2896 2755 Size: 141 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2543 2669 3043 3995 4592 5379 Size: 393 665 246 126 374 952 597 787 Cuts at: 5379 5647 1239 Size: 268 1524 Fragments arranged by size: 1524 952 787 665 597 393 374 268 246 126 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2384 Size: 5932 EcoO109I rG'GnC_Cy Cuts at: 2476 2683 3010 3160 2476 Size: 207 327 150 5248 Fragments arranged by size: 5248 327 207 150 EcoRI G'AATT_C Cuts at: 3241 3619 3241 Size: 378 5554 FspI TGC'GCA Cuts at: 2411 4438 2411 Size: 2027 3905 HaeII r_GCGC'y Cuts at: 1678 2839 5079 1678 Size: 1161 2240 2531 Fragments arranged by size: 2531 2240 1161 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3075 3107 3186 3218 4573 Size: 32 135 32 1503 32 79 32 1355 Cuts at: 4573 4605 4647 4679 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1503 1355 135 79 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3276 3522 669 Size: 988 281 1338 246 3079 Fragments arranged by size: 3079 1338 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5932 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KasI G'GCGC_C Cuts at: 2835 2835 Size: 5932 KpnI G_GTAC'C Cuts at: 2245 3257 2245 Size: 1012 4920 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5046 5230 1305 Size: 3741 184 2007 Fragments arranged by size: 3741 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4110 Fragments arranged by size: 4110 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2978 3143 3890 4249 4408 518 Size: 648 913 899 165 747 359 159 2042 Fragments arranged by size: 2042 913 899 747 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2468 2731 2963 3184 3348 4025 5173 5418 Size: 862 263 232 221 164 677 1148 245 Cuts at: 5418 1606 Size: 2120 Fragments arranged by size: 2120 1148 862 677 263 245 232 221 164 NarI GG'CG_CC Cuts at: 2836 2836 Size: 5932 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4441 Fragments arranged by size: 4441 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 3290 4835 1132 Size: 97 2061 1545 2229 Fragments arranged by size: 2229 2061 1545 97 PasI CC'CwG_GG Cuts at: 1348 2783 3220 1348 Size: 1435 437 4060 Fragments arranged by size: 4060 1435 437 PciI A'CATG_T Cuts at: 4831 4831 Size: 5932 PflMI CCAn_nnn'nTGG Cuts at: 1108 2576 2817 1108 Size: 1468 241 4223 Fragments arranged by size: 4223 1468 241 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2683 3010 2476 Size: 207 327 5398 Fragments arranged by size: 5398 327 207 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5932 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PspOMI G'GGCC_C Cuts at: 2662 2662 Size: 5932 PstI C_TGCA'G Cuts at: 830 3284 830 Size: 2454 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 PvuII CAG'CTG Cuts at: 2731 2963 3184 2731 Size: 232 221 5479 Fragments arranged by size: 5479 232 221 SacI G_AGCT'C Cuts at: 2757 2898 2757 Size: 141 5791 SalI G'TCGA_C Cuts at: 1936 3274 1936 Size: 1338 4594 SapI GCTCTTCn'nnn_ Cuts at: 2903 2903 Size: 5932 SbfI CC_TGCA'GG Cuts at: 3284 3284 Size: 5932 ScaI AGT'ACT Cuts at: 4180 5892 4180 Size: 1712 4220 SfcI C'TryA_G Cuts at: 826 1045 2108 2422 3280 4415 5096 5287 826 Size: 219 1063 314 858 1135 681 191 1471 Fragments arranged by size: 1471 1135 1063 858 681 314 219 191 SfoI GGC'GCC Cuts at: 2837 2837 Size: 5932 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 2832 1700 Size: 1132 4800 SmaI CCC'GGG Cuts at: 1872 2741 3259 1872 Size: 869 518 4545 Fragments arranged by size: 4545 869 518 SmlI C'TyrA_G Cuts at: 820 1017 1943 4036 4937 5199 820 Size: 197 926 2093 901 262 1553 Fragments arranged by size: 2093 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 3290 1132 Size: 2158 3774 SspI AAT'ATT Cuts at: 5 52 3856 5 Size: 47 3804 2081 Fragments arranged by size: 3804 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5932 StyI C'CwwG_G Cuts at: 513 1065 2004 3029 3165 3371 513 Size: 552 939 1025 136 206 3074 Fragments arranged by size: 3074 1025 939 552 206 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2317 3959 3976 4129 4314 4759 325 Size: 1255 737 1642 17 153 185 445 1498 Fragments arranged by size: 1642 1498 1255 737 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 2334 2430 Size: 274 80 33 51 1443 282 75 96 Cuts at: 2430 4178 5890 96 Size: 1748 1712 138 Fragments arranged by size: 1748 1712 1443 282 274 138 96 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5892 5924 5892 Size: 32 5900 Tth111I GACn'n_nGTC Cuts at: 1316 2824 1316 Size: 1508 4424 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5932 XmaI C'CCGG_G Cuts at: 1870 2739 3257 1870 Size: 869 518 4545 Fragments arranged by size: 4545 869 518 XmnI GAAnn'nnTTC Cuts at: 4061 4061 Size: 5932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2013 3740 276 Size: 53 83 186 936 479 1727 2468 Fragments arranged by size: 2468 1727 936 479 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI