(Circular) (Six-base) MAPSORT of: pFN21AB7782.seq Check: 9796 from: 1 to: 6235 pFN21AB7782 6235 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3045 3045 Size: 6235 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3578 1937 Size: 1641 4594 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2126 2462 2465 2888 4298 5682 5950 Size: 416 78 336 3 423 1410 1384 268 Cuts at: 5950 1632 Size: 1917 Fragments arranged by size: 1917 1410 1384 423 416 336 268 78 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 2957 5066 2957 Size: 2109 4126 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 2960 1166 Size: 1794 4441 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3043 1184 Size: 1859 4376 AlwNI CAG_nnn'CTG Cuts at: 2664 2866 3978 5550 2664 Size: 202 1112 1572 3349 Fragments arranged by size: 3349 1572 1112 202 ApaI G_GGCC'C Cuts at: 2131 3281 3486 2131 Size: 1150 205 4880 Fragments arranged by size: 4880 1150 205 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 3544 3770 3922 6031 1493 Size: 2051 226 152 2109 1697 Fragments arranged by size: 2109 2051 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2674 2699 3560 1870 Size: 73 731 25 861 4545 Fragments arranged by size: 4545 861 731 73 25 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3216 3260 3329 3389 3523 Size: 325 217 627 1429 44 69 60 134 Cuts at: 3523 3556 5011 618 Size: 33 1455 1842 Fragments arranged by size: 1842 1455 1429 627 325 217 134 69 60 44 33 BanII G_rGCy'C Cuts at: 1391 2118 2131 2492 2712 3150 3281 3300 3486 Size: 727 13 361 220 438 131 19 186 Cuts at: 3486 3554 1391 Size: 68 4072 Fragments arranged by size: 4072 727 438 361 220 186 131 68 19 13 BbeI G_GCGC'C Cuts at: 3264 3393 3264 Size: 129 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2876 3049 3471 928 Size: 971 977 173 422 3692 Fragments arranged by size: 3692 977 971 422 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 1548 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2239 2716 Size: 107 122 71 631 605 337 230 477 Cuts at: 2716 3258 3363 4846 136 Size: 542 105 1483 1525 Fragments arranged by size: 1525 1483 631 605 542 477 337 230 122 107 105 71 BglII A'GATC_T Cuts at: 6230 6230 Size: 6235 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2131 2295 3012 3101 3221 3281 3334 3486 Size: 341 164 717 89 120 60 53 152 Cuts at: 3486 4296 5452 1790 Size: 810 1156 2573 Fragments arranged by size: 2573 1156 810 717 341 164 152 120 89 60 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 3966 4924 Size: 416 53 83 399 24 952 1597 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1597 958 952 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2524 4895 1239 Size: 878 407 2371 2579 Fragments arranged by size: 2579 2371 878 407 Bpu10I CC'TnA_GC Cuts at: 2160 3164 2160 Size: 1004 5231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 4360 5225 5523 2369 Size: 1991 865 298 3081 Fragments arranged by size: 3081 1991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2186 3017 4898 882 Size: 617 687 831 1881 2219 Fragments arranged by size: 2219 1881 831 687 617 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2510 3051 3180 3261 Size: 53 83 186 936 977 541 129 81 Cuts at: 3261 3288 3390 4042 4424 5059 275 Size: 27 102 652 382 635 1451 Fragments arranged by size: 1451 977 936 652 635 541 382 186 129 102 83 81 53 27 BsaWI w'CCGG_w Cuts at: 2532 2957 4668 5066 5340 5487 5898 2532 Size: 425 1711 398 274 147 411 2869 Fragments arranged by size: 2869 1711 425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2083 2113 2477 2507 2609 Size: 30 165 30 680 30 364 30 102 Cuts at: 2609 2639 2672 2702 1178 Size: 30 33 30 4711 Fragments arranged by size: 4711 680 364 165 102 33 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3140 3455 3458 3461 3470 3473 1186 Size: 1954 315 3 3 9 3 3948 Fragments arranged by size: 3948 1954 315 9 3 3 3 BseYI C'CCAG_C Cuts at: 3274 3311 5438 5934 3274 Size: 37 2127 496 3575 Fragments arranged by size: 3575 2127 496 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2591 1163 Size: 573 426 429 4807 Fragments arranged by size: 4807 573 429 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2278 3685 4446 5474 1540 Size: 275 186 277 1407 761 1028 2301 Fragments arranged by size: 2301 1407 1028 761 277 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2337 4296 4381 5452 2118 Size: 219 1959 85 1071 2901 Fragments arranged by size: 2901 1959 1071 219 85 BsmI GAATG_Cn' Cuts at: 3746 3839 3746 Size: 93 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2131 2295 2337 2492 2712 3012 Size: 399 328 13 164 42 155 220 300 Cuts at: 3012 3101 3150 3221 3281 3300 3334 3486 3554 Size: 89 49 71 60 19 34 152 68 Cuts at: 3554 4296 4381 5452 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 300 220 164 155 152 89 85 71 68 60 49 42 34 19 13 BspEI T'CCGG_A Cuts at: 2532 5898 2532 Size: 3366 2869 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2563 3035 3045 3072 3590 844 Size: 1719 472 10 27 518 3489 Fragments arranged by size: 3489 1719 518 472 27 10 BsrBI CCG'CTC Cuts at: 1394 1823 3327 4122 1394 Size: 429 1504 795 3507 Fragments arranged by size: 3507 1504 795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4730 4904 65 Size: 1183 3482 174 1396 Fragments arranged by size: 3482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2238 2282 2957 3526 4879 5066 1700 Size: 110 428 44 675 569 1353 187 2869 Fragments arranged by size: 2869 1353 675 569 428 187 110 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 2111 3211 3269 4295 5307 2111 Size: 1100 58 1026 1012 3039 Fragments arranged by size: 3039 1100 1026 1012 58 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6235 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 6235 BstYI r'GATC_y Cuts at: 1972 3159 3565 3932 4317 4334 5786 5872 5884 Size: 1187 406 367 385 17 1452 86 12 Cuts at: 5884 6230 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1187 406 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2613 2613 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4744 Fragments arranged by size: 4744 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2623 487 Size: 683 56 1397 4099 Fragments arranged by size: 4099 1397 683 56 BtsI GCAGTG_nn' Cuts at: 3047 3751 4545 4565 3047 Size: 704 794 20 4717 Fragments arranged by size: 4717 794 704 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2502 2537 529 Size: 35 1938 35 4227 Fragments arranged by size: 4227 1938 35 35 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 3682 4571 Size: 54 1425 50 275 18 933 919 889 Cuts at: 4571 6200 8 Size: 1629 43 Fragments arranged by size: 1629 1425 933 919 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3447 3471 4165 1353 Size: 2094 24 694 3423 Fragments arranged by size: 3423 2094 694 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 2387 4822 5196 5342 387 Size: 1064 138 438 360 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 438 374 360 146 138 EcoICRI GAG'CTC Cuts at: 2116 2116 Size: 6235 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2117 2126 2462 2465 2524 2888 Size: 393 416 69 9 336 3 59 364 Cuts at: 2888 4298 4895 5682 5950 1239 Size: 1410 597 787 268 1524 Fragments arranged by size: 1524 1410 787 597 416 393 364 336 268 69 59 9 3 EcoO109I rG'GnC_Cy Cuts at: 2127 2128 2312 3278 3482 3483 3517 2127 Size: 1 184 966 204 1 34 4845 Fragments arranged by size: 4845 966 204 184 34 1 1 EcoRI G'AATT_C Cuts at: 3544 3922 3544 Size: 378 5857 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 2289 3264 3393 3499 5382 1678 Size: 611 975 129 106 1883 2531 Fragments arranged by size: 2531 1883 975 611 129 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3407 3439 4876 4908 4950 Size: 32 135 32 1835 32 1437 32 42 Cuts at: 4950 4982 1373 Size: 32 2626 Fragments arranged by size: 2626 1835 1437 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2653 3319 3579 3825 669 Size: 988 281 715 666 260 246 3079 Fragments arranged by size: 3079 988 715 666 281 260 246 HindIII A'AGCT_T Cuts at: 748 2477 748 Size: 1729 4506 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KasI G'GCGC_C Cuts at: 3260 3389 3260 Size: 129 6106 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 5349 5533 1305 Size: 777 3267 184 2007 Fragments arranged by size: 3267 2007 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2642 2960 3092 3269 4193 4552 Size: 648 873 603 318 132 177 924 359 Cuts at: 4552 4711 518 Size: 159 2042 Fragments arranged by size: 2042 924 873 648 603 359 318 177 159 132 MspA1I CmG'CkG Cuts at: 1606 2137 2305 2607 2839 2986 3246 3501 3651 Size: 531 168 302 232 147 260 255 150 Cuts at: 3651 4328 5476 5721 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 302 260 255 245 232 168 150 147 NaeI GCC'GGC Cuts at: 2284 3528 2284 Size: 1244 4991 NarI GG'CG_CC Cuts at: 3261 3390 3261 Size: 129 6106 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4744 Fragments arranged by size: 4744 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NgoMIV G'CCGG_C Cuts at: 2282 3526 2282 Size: 1244 4991 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3593 5138 1132 Size: 97 2364 1545 2229 Fragments arranged by size: 2364 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3334 1348 Size: 1986 4249 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6235 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 2127 3277 3482 2127 Size: 1150 205 4880 Fragments arranged by size: 4880 1150 205 PstI C_TGCA'G Cuts at: 830 2553 2862 3042 3069 3251 3587 830 Size: 1723 309 180 27 182 336 3478 Fragments arranged by size: 3478 1723 336 309 182 180 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2137 2607 2839 3246 2137 Size: 470 232 407 5126 Fragments arranged by size: 5126 470 407 232 SacI G_AGCT'C Cuts at: 2118 2118 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 2348 4483 6195 2348 Size: 2135 1712 2388 Fragments arranged by size: 2388 2135 1712 SexAI A'CCwGG_T Cuts at: 2826 3198 2826 Size: 372 5863 SfcI C'TryA_G Cuts at: 826 1045 2152 2549 2858 3038 3065 3247 3583 Size: 219 1107 397 309 180 27 182 336 Cuts at: 3583 4718 5399 5590 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1107 681 397 336 309 219 191 182 180 27 SfoI GGC'GCC Cuts at: 3262 3391 3262 Size: 129 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 2957 1700 Size: 1257 4978 SmaI CCC'GGG Cuts at: 1872 2676 2701 3562 1872 Size: 804 25 861 4545 Fragments arranged by size: 4545 861 804 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 4339 5240 5502 820 Size: 197 926 441 1955 901 262 1553 Fragments arranged by size: 1955 1553 926 901 441 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 2320 4159 5 Size: 47 2268 1839 2081 Fragments arranged by size: 2268 2081 1839 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2004 3674 513 Size: 552 939 1670 3074 Fragments arranged by size: 3074 1670 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2756 4262 4279 4432 4617 5062 325 Size: 1255 1176 1506 17 153 185 445 1498 Fragments arranged by size: 1506 1498 1255 1176 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 4481 6193 Size: 274 80 33 51 1443 369 2135 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 2135 1712 1443 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2083 2115 2477 2509 3197 3229 6195 6227 2083 Size: 32 362 32 688 32 2966 32 2091 Fragments arranged by size: 2966 2091 688 362 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3053 1316 Size: 1737 4498 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 3271 2302 Size: 969 5266 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 2674 2699 3560 1870 Size: 804 25 861 4545 Fragments arranged by size: 4545 861 804 25 XmnI GAAnn'nnTTC Cuts at: 4364 4364 Size: 6235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI