Restriction map for
pFN21AB7084
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eam1105I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HinfI
HpaI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 106 CGCG CG^CG
AcyI 203 GGCGCC G[GA]^CG[CT]C
AlwI 271 GATCC GGATC(4/5)
Aor51HI 86 AGCGCT AGC^GCT
ApaI 344 GGGCCC GGGCC^C
AvaI 358 CTCGGG C^[CT]CG[GA]G
AvrII 297 CCTAGG C^CTAGG
BanI 203 GGCGCC G^G[CT][GA]CC
BbeI 203 GGCGCC GGCGC^C
BfaI 298 CTAG C^TAG
BlnI 297 CCTAGG C^CTAGG
BlpI 156 GCTCAGC GC^T[ACGT]AGC
BpmI 43 CTGGAG CTGGAG(16/14)
Bpu1102I 156 GCTCAGC GC^T[ACGT]AGC
BsaHI 203 GGCGCC G[GA]^CG[CT]C
BsiEI 141 CGGCCG CG[GA][CT]^CG
BsoBI 358 CTCGGG C^[CT]CG[GA]G
BsrFI 143 GCCGGC [GA]^CCGG[CT]
BssSI 373 CTCGTG CACGAG(-5/-1)
BstEII 225 GGTGACC G^GT[ACGT]ACC
BstF5I 330 CATCC GGATG(2/0)
BstPI 225 GGTGACC G^GT[ACGT]ACC
BstUI 106 CGCG CG^CG
BstYI 270 AGATCC [GA]^GATC[CT]
CelII 156 GCTCAGC GC^T[ACGT]AGC
Cfr10I 143 GCCGGC [GA]^CCGG[CT]
DdeI 157 CTCAG C^T[ACGT]AG
DraII 367 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 369 GACCCTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 179 CCGTGG C^C[GA][CT]GG
EaeI 141 CGGCCG [CT]^GGCC[GA]
EagI 141 CGGCCG C^GGCCG
EarI 118 CTCTTC CTCTTC(1/4)
EclXI 141 CGGCCG C^GGCCG
Eco47III 86 AGCGCT AGC^GCT
Eco52I 141 CGGCCG C^GGCCG
EcoO109I 367 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 225 GGTGACC G^GT[ACGT]ACC
FokI 330 CATCC GGATG(9/13)
FseI 142 GGCCGGCC GGCCGG^CC
HgaI 92 GCGTC GACGC(5/10)
Hin1I 203 GGCGCC G[GA]^CG[CT]C
KasI 203 GGCGCC G^GCGCC
Ksp632I 118 CTCTTC CTCTTC(1/4)
MaeI 298 CTAG C^TAG
MaeIII 226 GTGAC ^GT[ACGT]AC
MflI 270 AGATCC [GA]^GATC[CT]
MslI 219 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 106 CGCG CG^CG
NaeI 143 GCCGGC GCC^GGC
NarI 203 GGCGCC GG^CGCC
NgoMIV 143 GCCGGC G^CCGGC
PpuMI 367 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 70 CAGCTG CAG^CTG
SacI 154 GAGCTC GAGCT^C
SapI 117 GCTCTTC GCTCTTC(1/4)
SfaNI 170 GCATC GCATC(5/9)
SfoI 203 GGCGCC GGC^GCC
SmlI 28 CTCAAG C^T[CT][GA]AG
StuI 300 AGGCCT AGG^CCT
Tsp45I 226 GTGAC ^GT[GC]AC
TspRI 338 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 270 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 362 GGACC G^G[AT]CC
368 GGACC
BanII 154 GAGCTC G[GA]GC[CT]^C
344 GGGCCC
BsmFI 361 GGGAC GGGAC(10/14)
367 GGGAC
BstNI 264 CCTGG CC^[AT]GG
303 CCTGG
EcoRII 264 CCTGG ^CC[AT]GG
303 CCTGG
HaeII 86 AGCGCT [GA]GCGC^[CT]
203 GGCGCC
MboII 268 GAAGA GAAGA(8/7)
119 TCTTC
MvaI 264 CCTGG CC^[AT]GG
303 CCTGG
PstI 67 CTGCAG CTGCA^G
112 CTGCAG
SfcI 67 CTGCAG C^T[GA][CT]AG
112 CTGCAG
TaqI 122 TCGA T^CGA
248 TCGA
TthHB8I 122 TCGA T^CGA
248 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AspHI BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BssKI Cac8I CfoI Cfr13I
DpnII EcoT14I FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MnlI MspA1I MspI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI StyI TseI