Restriction map for
pFN21AB7084
(Linear) (Six-base) MAPSORT of: KIBB7084.seq Check: 2472 from: 1 to: 381
KIBB7084.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AfeI AGC'GCT
Cuts at: 0 88 381
Size: 88 293
AleI CACnn'nnGTG
Cuts at: 0 223 381
Size: 223 158
ApaI G_GGCC'C
Cuts at: 0 348 381
Size: 348 33
AvaI C'yCGr_G
Cuts at: 0 358 381
Size: 358 23
AvrII C'CTAG_G
Cuts at: 0 297 381
Size: 297 84
BanI G'GyrC_C
Cuts at: 0 203 381
Size: 203 178
BanII G_rGCy'C
Cuts at: 0 158 348 381
Size: 158 190 33
Fragments arranged by size:
190 158 33
BbeI G_GCGC'C
Cuts at: 0 207 381
Size: 207 174
BlpI GC'TnA_GC
Cuts at: 0 157 381
Size: 157 224
Bme1580I G_kGCm'C
Cuts at: 0 136 348 381
Size: 136 212 33
Fragments arranged by size:
212 136 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 381
Size: 64 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 381
Size: 13 368
BsaHI Gr'CG_yC
Cuts at: 0 204 381
Size: 204 177
BsiEI CG_ry'CG
Cuts at: 0 144 381
Size: 144 237
BsiHKAI G_wGCw'C
Cuts at: 0 29 158 199 381
Size: 29 129 41 182
Fragments arranged by size:
182 129 41 29
Bsp1286I G_dGCh'C
Cuts at: 0 29 136 158 199 348 381
Size: 29 107 22 41 149 33
Fragments arranged by size:
149 107 41 33 29 22
BsrFI r'CCGG_y
Cuts at: 0 143 381
Size: 143 238
BssSI C'ACGA_G
Cuts at: 0 373 381
Size: 373 8
BstEII G'GTnAC_C
Cuts at: 0 225 381
Size: 225 156
BstYI r'GATC_y
Cuts at: 0 270 381
Size: 270 111
BtgI C'CryG_G
Cuts at: 0 179 381
Size: 179 202
DrdI GACnn_nn'nnGTC
Cuts at: 0 375 381
Size: 375 6
EaeI y'GGCC_r
Cuts at: 0 141 381
Size: 141 240
EagI C'GGCC_G
Cuts at: 0 141 381
Size: 141 240
EarI CTCTTCn'nnn_
Cuts at: 0 124 381
Size: 124 257
EcoICRI GAG'CTC
Cuts at: 0 156 381
Size: 156 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 381
Size: 64 317
EcoO109I rG'GnC_Cy
Cuts at: 0 368 381
Size: 368 13
FseI GG_CCGG'CC
Cuts at: 0 147 381
Size: 147 234
HaeII r_GCGC'y
Cuts at: 0 90 207 381
Size: 90 117 174
Fragments arranged by size:
174 117 90
KasI G'GCGC_C
Cuts at: 0 203 381
Size: 203 178
MslI CAynn'nnrTG
Cuts at: 0 223 381
Size: 223 158
MspA1I CmG'CkG
Cuts at: 0 56 72 257 381
Size: 56 16 185 124
Fragments arranged by size:
185 124 56 16
NaeI GCC'GGC
Cuts at: 0 145 381
Size: 145 236
NarI GG'CG_CC
Cuts at: 0 204 381
Size: 204 177
NgoMIV G'CCGG_C
Cuts at: 0 143 381
Size: 143 238
PpuMI rG'GwC_Cy
Cuts at: 0 368 381
Size: 368 13
PspOMI G'GGCC_C
Cuts at: 0 344 381
Size: 344 37
PstI C_TGCA'G
Cuts at: 0 71 116 381
Size: 71 45 265
Fragments arranged by size:
265 71 45
PvuII CAG'CTG
Cuts at: 0 72 381
Size: 72 309
SacI G_AGCT'C
Cuts at: 0 158 381
Size: 158 223
SanDI GG'GwC_CC
Cuts at: 0 368 381
Size: 368 13
SapI GCTCTTCn'nnn_
Cuts at: 0 124 381
Size: 124 257
SfcI C'TryA_G
Cuts at: 0 67 112 381
Size: 67 45 269
Fragments arranged by size:
269 67 45
SfoI GGC'GCC
Cuts at: 0 205 381
Size: 205 176
SmlI C'TyrA_G
Cuts at: 0 28 381
Size: 28 353
StuI AGG'CCT
Cuts at: 0 302 381
Size: 302 79
StyI C'CwwG_G
Cuts at: 0 128 149 297 381
Size: 128 21 148 84
Fragments arranged by size:
148 128 84 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 381
Size: 244 137
Enzymes that do cut:
AfeI AleI ApaI AvaI AvrII BanI BanII BbeI
BlpI Bme1580I BpmI BpuEI BsaHI BsiEI BsiHKAI Bsp1286I
BsrFI BssSI BstEII BstYI BtgI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I FseI HaeII KasI MslI
MspA1I NaeI NarI NgoMIV PpuMI PspOMI PstI PvuII
SacI SanDI SapI SfcI SfoI SmlI StuI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoNI
EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI